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    • 3. 发明授权
    • Method and apparatus for analysis by liquid chromatography
    • 用于液相色谱分析的方法和装置
    • US08104330B2
    • 2012-01-31
    • US12278867
    • 2006-02-09
    • Morimasa HayashiYoshihiro Hayakawa
    • Morimasa HayashiYoshihiro Hayakawa
    • G01N30/30
    • G01N30/30G01N30/34G01N2030/3007G01N2030/347
    • In order to obtain a temperature gradient elution method which is rapid and accurate, a mobile phase (1) is supplied through two mobile-phase flow channels (2a) and (2b), mixed together, and introduced into the column (15) while the column (15) is made adiabatic. In this operation, the temperature of the mobile phase in one mobile-phase flow channel, i.e., the channel (2a), is regulated to a constant temperature higher than the upper limit of the target temperature range to be obtained in the column (15), while the temperature of the mobile phase in the other mobile-phase flow channel, i.e., the channel (2b), is regulated to a constant temperature lower than the lower limit of the target temperature range in the column (15). By controlling the flow rates in the two mobile-phase flow channels (2a) and (2b), the proportion in which these mobile-phase portions are mixed is changed with time to thereby change the temperature of the mobile phase in the column (15) with time. The temperature gradient elution method is thus conducted.
    • 为了获得快速准确的温度梯度洗脱方法,将流动相(1)通过两个流动相流动通道(2a)和(2b)供应,混合在一起,并引入塔(15),同时 柱(15)是绝热的。 在该操作中,一个流动相流路(即,通道(2a))中的流动相的温度被调节到高于在列(15)中获得的目标温度范围的上限的恒定温度 ),而另一流动相流路(即,通道(2b))中的流动相的温度被调节到低于列(15)中的目标温度范围的下限的恒定温度。 通过控制两个流动相流动通道(2a)和(2b)中的流速,混合这些流动相部分的比例随时间而改变,从而改变塔(15)中流动相的温度 ) 随着时间的推移。 因此进行温度梯度洗脱法。