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1. 发明授权

US09139617B2 Method for detecting antibody against SITH-1 in biological sample 有权
标题翻译：检测生物样品中SITH-1抗体的方法
公开(公告)号：US09139617B2
公开(公告)日：2015-09-22
申请号：US13257754
申请日：2010-03-31
申请人： Yoshimitsu Takakura , Naomi Oka , Kazuhiro Kondo , Nobuyuki Kobayashi
发明人： Yoshimitsu Takakura , Naomi Oka , Kazuhiro Kondo , Nobuyuki Kobayashi
IPC分类号： C07K14/005 , G01N33/569 , G01N33/68
CPC分类号： C07K14/005 , C07K2319/70 , C12N2710/16522 , G01N33/56994 , G01N33/6854 , G01N2333/03 , G01N2469/20
摘要： The present invention relates to a method for detecting an antibody against a small protein encoded by the intermediate stage transcript of HHV-6 (SITH-1) in a biological sample. The method of the present invention includes the following steps: 1) providing the SITH-1 protein; 2) binding the SITH-1 protein provided in step 1) to a carrier; and 3) contacting the biological sample with the SITH-1 protein-bound carrier prepared in step 2) to detect the SITH-1 protein antibody.
摘要翻译：本发明涉及生物样品中HHV-6（SITH-1）的中间转录物编码的小蛋白质的抗体检测方法。本发明的方法包括以下步骤：1）提供SITH-1蛋白; 2）将步骤1）中提供的SITH-1蛋白质结合到载体上; 和3）使生物样品与步骤2）中制备的SITH-1蛋白结合载体接触以检测SITH-1蛋白抗体。

2. 发明申请

US20120088313A1 METHOD FOR DETECTING SUBSTANCE IN BIOLOGICAL SAMPLE 有权
标题翻译：检测生物样品中物质的方法
公开(公告)号：US20120088313A1
公开(公告)日：2012-04-12
申请号：US13258247
申请日：2010-03-31
申请人： Yoshimitsu Takakura , Naomi Oka , Kazuhiro Kondo
发明人： Yoshimitsu Takakura , Naomi Oka , Kazuhiro Kondo
IPC分类号： G01N33/566 , B01L99/00
CPC分类号： G01N33/54393 , G01N33/54306 , G01N33/54353 , G01N33/56994 , G01N33/6854 , G01N2333/035 , G01N2333/36
摘要： The present invention provides a method for detecting a substance in a biological sample, a carrier for using in the method, and a kit. The method of the present invention includes 1) providing a carrier on which a biotin-binding protein is bound and providing a biotinylated protein by biotinylating a protein that specifically binds to a substance to be detected; 2) binding the biotinylated protein to the carrier provided in step 1) to produce a biotinylated protein-bound carrier; 3) mixing (a) a biological sample, and (b-i) a cell homogenate extract prepared from cells of the same species as that of the host cells used for expressing, for example, the biotin-binding protein in step 1), and a biotin-binding protein, or (b-ii) a cell homogenate extract prepared from cells of the same species as that of the host cells used for expressing, for example, the biotin-binding protein in step 1) and genetically engineered to express a biotin-binding protein, and adding the mixture to the biotinylated protein-bound carrier produced in step 2); and 4) detecting the substance specifically bound to the biotinylated protein.
摘要翻译：本发明提供一种用于检测生物样品中的物质的方法，该方法中使用的载体和试剂盒。本发明的方法包括：1）提供结合有生物素结合蛋白的载体，通过生物素化与待检测物质特异性结合的蛋白质提供生物素化蛋白质; 2）将生物素化蛋白质与步骤1）中提供的载体结合以产生生物素化的蛋白质结合的载体; 3）混合（a）生物样品和（bi）由与用于在步骤1）中表达例如生物素结合蛋白的宿主细胞相同种类的细胞制备的细胞匀浆提取物，和生物素结合蛋白，或（b-ii）从与用于表达例如步骤1中的生物素结合蛋白的宿主细胞相同种类的细胞制备的细胞匀浆提取物），并进行遗传工程化以表达生物素结合蛋白，并将混合物加入到步骤2）中产生的生物素化蛋白质结合的载体上; 和4）检测与生物素化蛋白质特异结合的物质。

3. 发明申请

US20120064543A1 METHOD FOR DETECTING SUBSTANCE IN BIOLOGICAL SAMPLE 审中-公开
标题翻译：检测生物样品中物质的方法
公开(公告)号：US20120064543A1
公开(公告)日：2012-03-15
申请号：US13254128
申请日：2010-03-02
申请人： Yoshimitsu Takakura , Naomi Oka , Kazuhiro Kondo
发明人： Yoshimitsu Takakura , Naomi Oka , Kazuhiro Kondo
IPC分类号： G01N33/82 , C07K19/00
CPC分类号： G01N33/54393
摘要： The present invention provides a method for detecting a substance in a biological sample, a carrier for using in the method, and a kit. The method of the present invention according to an embodiment includes: 1) providing a carrier on which biotin is bound and providing a fusion protein between a protein that specifically binds to a substance to be detected and a biotin-binding protein; 2) binding the fusion protein to the carrier provided in step 1) through binding between biotin and the biotin-binding protein to produce a fusion protein-bound carrier; 3) mixing (a) a biological sample, and (b-i) a cell homogenate extract prepared from cells of the same species as the host cells used for expressing the fusion protein in step 1), and a biotin-binding protein, or (b-ii) a cell homogenate extract prepared from cells of the same species as the host cells used for expressing the fusion protein in step 1), and genetically engineered to express a biotin-binding protein, and adding the mixture to the fusion protein-bound carrier produced in step 2); and 4) detecting a substance that has bound to the protein that specifically binds to a substance to be detected in the fusion protein.
摘要翻译：本发明提供一种用于检测生物样品中的物质的方法，该方法中使用的载体和试剂盒。根据一个实施方案的本发明的方法包括：1）提供结合有生物素的载体，并在特异性结合待检物质与生物素结合蛋白之间提供融合蛋白; 2）通过生物素与生物素结合蛋白之间的结合将融合蛋白与步骤1）中提供的载体结合以产生融合蛋白结合的载体; 3）混合（a）生物样品，和（bi）从与步骤1）中用于表达融合蛋白的宿主细胞相同种类的细胞制备的细胞匀浆提取物和生物素结合蛋白，或（b -ii）从与步骤1）中用于表达融合蛋白的宿主细胞相同物种的细胞制备的细胞匀浆提取物，并进行遗传工程改造以表达生物素结合蛋白，并将所述混合物加入融合蛋白结合载体在步骤2）中生产; 以及4）检测与融合蛋白中特异性结合待检测物质的蛋白质结合的物质。

4. 发明授权

US09034590B2 Method for detecting substance in biological sample 有权
标题翻译：生物样品中物质检测方法
公开(公告)号：US09034590B2
公开(公告)日：2015-05-19
申请号：US13258247
申请日：2010-03-31
申请人： Yoshimitsu Takakura , Naomi Oka , Kazuhiro Kondo
发明人： Yoshimitsu Takakura , Naomi Oka , Kazuhiro Kondo
IPC分类号： G01N33/543 , G01N33/569 , G01N33/68
CPC分类号： G01N33/54393 , G01N33/54306 , G01N33/54353 , G01N33/56994 , G01N33/6854 , G01N2333/035 , G01N2333/36
摘要： The present invention provides a method for detecting a substance in a biological sample, a carrier for using in the method, and a kit. The method of the present invention includes 1) providing a carrier on which a biotin-binding protein is bound and providing a biotinylated protein by biotinylating a protein that specifically binds to a substance to be detected; 2) binding the biotinylated protein to the carrier provided in step 1) to produce a biotinylated protein-bound carrier; 3) mixing (a) a biological sample, and (b-i) a cell homogenate extract prepared from cells of the same species as that of the host cells used for expressing, for example, the biotin-binding protein in step 1), and a biotin-binding protein, or (b-ii) a cell homogenate extract prepared from cells of the same species as that of the host cells used for expressing, for example, the biotin-binding protein in step 1) and genetically engineered to express a biotin-binding protein, and adding the mixture to the biotinylated protein-bound carrier produced in step 2); and 4) detecting the substance specifically bound to the biotinylated protein.
摘要翻译：本发明提供一种用于检测生物样品中的物质的方法，该方法中使用的载体和试剂盒。本发明的方法包括：1）提供结合有生物素结合蛋白的载体，通过生物素化与待检测物质特异性结合的蛋白质提供生物素化蛋白质; 2）将生物素化蛋白质与步骤1）中提供的载体结合以产生生物素化的蛋白质结合的载体; 3）混合（a）生物样品和（bi）由与用于在步骤1）中表达例如生物素结合蛋白的宿主细胞相同种类的细胞制备的细胞匀浆提取物，和生物素结合蛋白，或（b-ii）从与用于表达例如步骤1中的生物素结合蛋白的宿主细胞相同种类的细胞制备的细胞匀浆提取物），并进行遗传工程化以表达生物素结合蛋白，并将混合物加入到步骤2）中产生的生物素化蛋白质结合的载体上; 和4）检测与生物素化蛋白质特异结合的物质。

5. 发明申请

US20120107842A1 METHOD FOR DETECTING ANTIBODY AGAINST SITH-1 IN BIOLOGICAL SAMPLE 有权
标题翻译：检测生物样品中SITH-1抗体的方法
公开(公告)号：US20120107842A1
公开(公告)日：2012-05-03
申请号：US13257754
申请日：2010-03-31
申请人： Yoshimitsu Takakura , Naomi Oka , Kazuhiro Kondo , Nobuyuki Koba Yashi
发明人： Yoshimitsu Takakura , Naomi Oka , Kazuhiro Kondo , Nobuyuki Koba Yashi
IPC分类号： G01N33/566 , C07K17/00 , G01N33/82
CPC分类号： C07K14/005 , C07K2319/70 , C12N2710/16522 , G01N33/56994 , G01N33/6854 , G01N2333/03 , G01N2469/20
摘要： The present invention provides a method for detecting an antibody against a small protein encoded by the intermediate stage transcript of HHV-6 (SITH-1) in a biological sample.The method of the present invention comprises: 1) providing the SITH-1 protein; 2) binding the SITH-1 protein provided in step 1) to a carrier; and 3) contacting the biological sample with the SITH-1 protein-bound carrier prepared in step 2) to detect the SITH-1 protein antibody.
摘要翻译：本发明提供了检测生物样品中由HHV-6（SITH-1）的中间转录物编码的小蛋白质的抗体的方法。本发明的方法包括：1）提供SITH-1蛋白; 2）将步骤1）中提供的SITH-1蛋白质结合到载体上; 和3）使生物样品与步骤2）中制备的SITH-1蛋白结合载体接触以检测SITH-1蛋白抗体。

6. 发明授权

US08343727B2 Method of binding proteins to carriers by making use of tamavidins 失效
标题翻译：通过使用塔非那定将蛋白质与载体结合的方法
公开(公告)号：US08343727B2
公开(公告)日：2013-01-01
申请号：US12675524
申请日：2008-08-28
申请人： Yoshimitsu Takakura , Masako Ichikawa , Satoru Usami , Takeshi Yamamoto , Hiroshi Tsukamoto , Hitomi Kajiwara , Naomi Oka
发明人： Yoshimitsu Takakura , Masako Ichikawa , Satoru Usami , Takeshi Yamamoto , Hiroshi Tsukamoto , Hitomi Kajiwara , Naomi Oka
IPC分类号： G01N33/53
CPC分类号： C07K17/06 , C07K14/375 , C07K2319/20 , G01N2333/36
摘要： The present invention provides a method of binding a protein to a carrier in such a way that the protein is not impaired in its function but can be allowed to act more efficiently than when it is bound directly.The method of the present invention for binding a protein to a carrier comprises: preparing a biotin-bound carrier; preparing a fusion protein having the protein bound to a tamavidin; and binding the protein to the carrier via tamavidin-biotin bonds.
摘要翻译：本发明提供了将蛋白质与载体结合的方法，使得蛋白质在其功能上不受损害，但可以比直接结合时更有效地起作用。将蛋白质与载体结合的本发明的方法包括：制备生物素结合的载体; 制备具有与蛋白糊精结合的蛋白质的融合蛋白; 并通过蛋白酶 - 生物素键将蛋白质与载体结合。

7. 发明申请

US20100311076A1 METHOD OF BINDING PROTEINS TO CARRIERS BY MAKING USE OF TAMAVIDINS 失效
标题翻译：通过使用TAMAVIDINS将蛋白质与载体结合的方法
公开(公告)号：US20100311076A1
公开(公告)日：2010-12-09
申请号：US12675524
申请日：2008-08-28
申请人： Yoshimitsu Takakura , Masako Ichikawa , Satoru Usami , Takeshi Yamamoto , Hiroshi Tsukamoto , Hitomi Kajiwara , Naomi Oka
发明人： Yoshimitsu Takakura , Masako Ichikawa , Satoru Usami , Takeshi Yamamoto , Hiroshi Tsukamoto , Hitomi Kajiwara , Naomi Oka
IPC分类号： G01N33/53 , C12N15/63 , C07K14/00 , C07K16/00 , C12N9/00
CPC分类号： C07K17/06 , C07K14/375 , C07K2319/20 , G01N2333/36
摘要： The present invention provides a method of binding a protein to a carrier in such a way that the protein is not impaired in its function but can be allowed to act more efficiently than when it is bound directly.The method of the present invention for binding a protein to a carrier comprises: preparing a biotin-bound carrier; preparing a fusion protein having the protein bound to a tamavidin; and binding the protein to the carrier via tamavidin-biotin bonds.
摘要翻译：本发明提供了将蛋白质与载体结合的方法，使得蛋白质在其功能上不受损害，但可以比直接结合时更有效地起作用。将蛋白质与载体结合的本发明的方法包括：制备生物素结合的载体; 制备具有与蛋白糊精结合的蛋白质的融合蛋白; 并通过蛋白酶 - 生物素键将蛋白质与载体结合。

8. 发明申请

US20140242620A1 METHOD OF INHIBITING NON-SPECIFIC BINDING IN STEP OF DETECTING SUBSTANCE IN BIOLOGICAL SAMPLE, AND AGENT FOR USE IN THE METHOD 审中-公开
标题翻译：在生物样品中检测物质的步骤中抑制非特异性结合的方法以及用于该方法的试剂
公开(公告)号：US20140242620A1
公开(公告)日：2014-08-28
申请号：US14235982
申请日：2012-08-01
申请人： Yoshimitsu Takakura , Naomi Oka
发明人： Yoshimitsu Takakura , Naomi Oka
IPC分类号： G01N33/543 , C07K14/375 , C07K14/435 , C07K14/36
CPC分类号： G01N33/54393 , C07K14/36 , C07K14/37 , C07K14/375 , C07K14/435 , C07K14/465 , G01N33/5306
摘要： The present invention provides a method of inhibiting non-specific binding in a step of detecting a substance in a biological sample. The invention provides a method of inhibiting non-specific binding in a step of detecting a substance in a biological sample, the method comprising: bringing a biotin-binding protein monomer not substantially having biotin-binding ability into contact with the biological sample, wherein in the step of detecting, a substance capable of specifically binding to the substance to be detected is immobilized to a carrier through binding between biotin and the biotin-binding protein before or after binding of the substance to be detected to the substance capable of specifically binding to the substance to be detected.
摘要翻译：本发明提供了在检测生物样品中的物质的步骤中抑制非特异性结合的方法。本发明提供了在检测生物样品中的物质的步骤中抑制非特异性结合的方法，所述方法包括：将基本上不具有生物素结合能力的生物素结合蛋白单体与生物样品接触，其中在检测能够将待检测物质特异性结合的物质的步骤通过生物素与生物素结合蛋白之间的结合固定在载体上，在待检测物质结合之前或之后，与能够特异性结合的物质待检测物质。

9. 发明授权

US09573982B2 Modified tamavidin 有权
标题翻译：改性的tamavidin
公开(公告)号：US09573982B2
公开(公告)日：2017-02-21
申请号：US13885655
申请日：2011-12-28
申请人： Yoshimitsu Takakura , Naomi Oka
发明人： Yoshimitsu Takakura , Naomi Oka
IPC分类号： C07H21/04 , C07K1/00 , C07K14/00 , A23J1/18 , A23J1/00 , C12N15/00 , C07K14/37 , C07K14/375 , G01N33/53 , C07K14/465
CPC分类号： C07K14/37 , C07K14/375 , C07K14/465 , G01N33/53
摘要： The present invention provides modified tamabidin 2, which is a modified biotin-binding protein comprising an amino acid sequence represented by SEQ ID NO: 2, an amino acid sequence having one or more amino acid mutations in the amino acid sequence of SEQ ID NO: 2, or an amino acid sequence having an identity of not less than 80% to the amino acid sequence of SEQ ID NO: 2 and having biotin-binding activity, wherein an asparagine residue at position 115 of SEQ ID NO: 2 is replaced with cysteine. The modified biotin-binding protein has remarkable heat resistance.
摘要翻译：本发明提供了修饰的脒苷2，其是包含SEQ ID NO：2所示的氨基酸序列的修饰的生物素结合蛋白，在SEQ ID NO：2的氨基酸序列中具有一个或多个氨基酸突变的氨基酸序列。 2或与SEQ ID NO：2的氨基酸序列具有不小于80％同一性且具有生物素结合活性的氨基酸序列，其中SEQ ID NO：2的位置115处的天冬酰胺残基被置换为半胱氨酸。修饰的生物素结合蛋白具有显着的耐热性。

10. 发明申请

US20130309765A1 MODIFIED TAMAVIDIN 有权
标题翻译：改性AM ID啶
公开(公告)号：US20130309765A1
公开(公告)日：2013-11-21
申请号：US13885655
申请日：2011-12-28
申请人： Yoshimitsu Takakura , Naomi Oka
发明人： Yoshimitsu Takakura , Naomi Oka
IPC分类号： C07K14/37
CPC分类号： C07K14/37 , C07K14/375 , C07K14/465 , G01N33/53
摘要： The present invention provides modified tamabidin 2, which is a modified biotin-binding protein comprising an amino acid sequence represented by SEQ ID NO: 2, an amino acid sequence having one or more amino acid mutations in the amino acid sequence of SEQ ID NO: 2, or an amino acid sequence having an identity of not less than 80% to the amino acid sequence of SEQ ID NO: 2 and having biotin-binding activity, wherein an asparagine residue at position 115 of SEQ ID NO: 2 is replaced with cysteine. The modified biotin-binding protein has remarkable heat resistance.
摘要翻译：本发明提供了修饰的脒苷2，其是包含SEQ ID NO：2所示的氨基酸序列的修饰的生物素结合蛋白，在SEQ ID NO：2的氨基酸序列中具有一个或多个氨基酸突变的氨基酸序列。 2或与SEQ ID NO：2的氨基酸序列具有不小于80％同一性且具有生物素结合活性的氨基酸序列，其中SEQ ID NO：2的位置115处的天冬酰胺残基被置换为半胱氨酸。修饰的生物素结合蛋白具有显着的耐热性。

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