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1. 发明申请

US20080160617A1 AGENT FOR INDUCING EMBRYONIC STEM CELL TO ECTODERMAL CELL, METHOD FOR OBTAINING THE SAME, AND USE OF THE SAME 审中-公开
标题翻译：将胚胎干细胞诱导至细胞细胞的药剂，其获得方法及其用途
公开(公告)号：US20080160617A1
公开(公告)日：2008-07-03
申请号：US12030314
申请日：2008-02-13
申请人： Yoshiki Sasai , Hiroo Iwata , Yoshinobu Murakami , Mitsuo Sato , Masato Kobori , Keiichi Yano
发明人： Yoshiki Sasai , Hiroo Iwata , Yoshinobu Murakami , Mitsuo Sato , Masato Kobori , Keiichi Yano
IPC分类号： C12N15/87 , C12N5/06
CPC分类号： G01N33/5073 , A61K35/12 , A61K35/48 , A61K38/1709 , C12N5/0603 , C12N5/0619 , C12N2501/415 , C12N2501/90 , C12N2501/91 , C12N2502/13 , C12N2503/02 , C12N2506/02 , G01N33/5008 , G01N33/5023 , G01N33/5058 , G01N33/6896
摘要： A method for obtaining a solution having activity to induce differentiation of an embryonic stem cell into an ectodermal cell or ectoderm-derived cell, which comprises culturing a stromal cell in a culture comprising a polyanionic compound and recovering the culture; a solution having activity to induce differentiation of an embryonic stem cell into an ectodermal cell or ectoderm-derived cell, which is obtainable by the method; and an agent for inducing differentiation of an embryonic stem cell into an ectodermal cell or ectoderm-derived cell.
摘要翻译：一种获得具有诱导胚胎干细胞分化为外胚层细胞或外胚层衍生细胞的活性的溶液的方法，其包括在包含聚阴离子化合物的培养物中培养基质细胞并回收培养物; 具有诱导胚胎干细胞分化为外胚层细胞或外胚层衍生细胞的活性的溶液，其可通过该方法获得; 以及用于诱导胚胎干细胞分化为外胚层细胞或外胚层衍生细胞的药剂。

2. 发明申请

US20060281179A1 Inducer for differentiation of embryo stem cells into ectodermal cells method of obtaining the same and use thereof 审中-公开
标题翻译：用于将胚胎干细胞分化为外胚层细胞的诱导剂获得相同的用途和用途
公开(公告)号：US20060281179A1
公开(公告)日：2006-12-14
申请号：US10495567
申请日：2002-11-14
申请人： Yoshiki Sasai , Hiroo Iwata , Yoshinobu Murakami , Mitsuo Satoh , Masato Kobori , Keiichi Yano
发明人： Yoshiki Sasai , Hiroo Iwata , Yoshinobu Murakami , Mitsuo Satoh , Masato Kobori , Keiichi Yano
IPC分类号： C12N5/08 , C12N15/09 , C07K14/475
CPC分类号： G01N33/5073 , A61K35/12 , A61K35/48 , A61K38/1709 , C12N5/0603 , C12N5/0619 , C12N2501/415 , C12N2501/90 , C12N2501/91 , C12N2502/13 , C12N2503/02 , C12N2506/02 , G01N33/5008 , G01N33/5023 , G01N33/5058 , G01N33/6896
摘要： A method for obtaining a solution having activity to induce differentiation of an embryonic stem cell into an ectodermal cell or ectoderm-derived cell, which comprises culturing a stromal cell in a culture comprising a polyanionic compound and recovering the culture; a solution having activity to induce differentiation of an embryonic stem cell into an ectodermal cell or ectoderm-derived cell, which is obtainable by the method; and an agent for inducing differentiation of an embryonic stem cell into an ectodermal cell or ectoderm-derived cell.
摘要翻译：一种获得具有诱导胚胎干细胞分化为外胚层细胞或外胚层衍生细胞的活性的溶液的方法，其包括在包含聚阴离子化合物的培养物中培养基质细胞并回收培养物; 具有诱导胚胎干细胞分化为外胚层细胞或外胚层衍生细胞的活性的溶液，其可通过该方法获得; 以及用于诱导胚胎干细胞分化为外胚层细胞或外胚层衍生细胞的药剂。

3. 发明申请

US20080305544A1 Method of Producing Nerve Cell 审中-公开
标题翻译：产生神经细胞的方法
公开(公告)号：US20080305544A1
公开(公告)日：2008-12-11
申请号：US11571580
申请日：2005-07-06
申请人： Hiroo Iwata , Yoshiki Sasai , Hironori Yamazoe , Masato Kobori , Mitsuo Satoh , Keiichi Yano
发明人： Hiroo Iwata , Yoshiki Sasai , Hironori Yamazoe , Masato Kobori , Mitsuo Satoh , Keiichi Yano
IPC分类号： C12N5/06
CPC分类号： C12N5/0619 , C12N2500/38 , C12N2506/02
摘要： An Object of the present invention is to provide a process for producing a nerve cell by inducing differentiation of an embryonic stem cell, a method for inducing differentiation of the embryonic stem cell into a nerve cell, a medium to be used in the production process or differentiation induction method, or a method for improving purity of the nerve cell obtained by inducing differentiation of the embryonic stem cell. The present invention provides a process for producing a nerve cell which is applicable to treatment of neurodegenerative disease or the like easily, selectively or inexpensively by inducing differentiation induction of an embryonic stem cell using vitamin B12 or a salt thereof and heparin, a substance having heparin-like activity or a salt.
摘要翻译：本发明的目的是提供一种通过诱导胚胎干细胞的分化来产生神经细胞的方法，用于诱导将胚胎干细胞分化成神经细胞的方法，用于生产过程的培养基或分化诱导方法或通过诱导胚胎干细胞分化而获得的提高神经细胞的纯度的方法。本发明提供了通过使用维生素B12或其盐诱导胚胎干细胞的分化诱导和肝素（含有肝素的物质），可以容易地，选择性地或廉价地治疗神经变性疾病等的神经细胞的制造方法类活动或盐。

4. 发明授权

US07923246B2 Method of culturing embryonic stem cells with the use of amniotic membrane-origin factor 失效
标题翻译：使用羊膜来源因子培养胚胎干细胞的方法
公开(公告)号：US07923246B2
公开(公告)日：2011-04-12
申请号：US11569414
申请日：2005-05-27
申请人： Yoshiki Sasai , Morio Ueno , Shigeru Kinoshita
发明人： Yoshiki Sasai , Morio Ueno , Shigeru Kinoshita
IPC分类号： C12N5/00 , C12N5/02 , C12N5/071
CPC分类号： G01N33/5073 , C12N5/0606 , C12N5/0619 , C12N2506/02 , G01N33/5008 , G01N33/502 , G01N33/5058
摘要： The present invention provides a clinically applicable method of inducing differentiation of the embryonic stem cells. Specifically, the present invention provides a method of culturing embryonic stem cells, which comprises culturing embryonic stem cells in the presence of an amnion-derived factor, a cell culture obtained by the culture method, and a culture agent/culture kit of embryonic stem cells comprising an amnion-derived factor. The present invention also provides a method of screening an amnion-derived factor having an activity useful for culturing embryonic stem cells with the activity as an index, and an amniocyte-derived factor obtained by the screening method. Furthermore, the present invention provides a method of preparing amniocytes useful for culturing embryonic stem cells, amniocytes obtained by the preparation method, and the like.
摘要翻译：本发明提供诱导胚胎干细胞分化的临床适用方法。具体地，本发明提供培养胚胎干细胞的方法，其包括在羊膜来源的因子存在下培养胚胎干细胞，通过培养方法获得的细胞培养物和胚胎干细胞的培养试剂/培养试剂盒包括羊膜衍生因子。本发明还提供一种筛选具有活性作为指标培养胚胎干细胞的活性的羊膜衍生因子和通过筛选方法得到的羊膜细胞衍生因子的方法。此外，本发明提供了制备用于培养胚胎干细胞的羊膜细胞的方法，通过制备方法获得的羊膜细胞等。

5. 发明申请

US20100009442A1 Stem Cell Culture Medium and Method 审中-公开
标题翻译：干细胞培养培养基和方法
公开(公告)号：US20100009442A1
公开(公告)日：2010-01-14
申请号：US12442245
申请日：2007-09-24
申请人： Yoshiki Sasai , Kiichi Watanabe
发明人： Yoshiki Sasai , Kiichi Watanabe
IPC分类号： C12N5/08 , C12N5/06
CPC分类号： C12N5/0606 , C12N5/0603 , C12N2501/70 , C12N2501/999 , C12N2506/00
摘要： Stem cells such as embryonic stem cells (ES cells), including human ES cells, are cultured in a medium comprising a ROCK inhibitor, and a stem cell culture medium, optionally serum free, comprises a ROCK inhibitor.
摘要翻译：包括人ES细胞在内的干细胞例如胚胎干细胞（ES细胞）在包含ROCK抑制剂的培养基中培养，任选不含血清的干细胞培养基包含ROCK抑制剂。

6. 发明授权

US10793830B2 Method for producing retinal tissue in vitro 有权
公开(公告)号：US10793830B2
公开(公告)日：2020-10-06
申请号：US13508303
申请日：2010-11-05
申请人： Yoshiki Sasai , Mototsugu Eiraku
发明人： Yoshiki Sasai , Mototsugu Eiraku
IPC分类号： C12N5/0789 , C12N5/079 , C12N5/0797
摘要： Provided is a method of inducing the differentiation of a stem cell into nerve progenitor cells, comprising the step (1) of forming a homogenous aggregate of stem cells in a serum-free medium (1) and the step (2) of suspension-culturing the homogenous aggregate of stem cells in the presence of a basement membrane reference standard.

7. 发明授权

US08492147B2 Method of inducing the differentiation of embryonic stem cells into nerve by serum-free suspension culture 有权
标题翻译：通过无血清悬浮培养诱导胚胎干细胞分化为神经的方法
公开(公告)号：US08492147B2
公开(公告)日：2013-07-23
申请号：US11570579
申请日：2005-06-16
申请人： Yoshiki Sasai , Kiichi Watanabe
发明人： Yoshiki Sasai , Kiichi Watanabe
IPC分类号： C12N5/00 , C12N5/02
CPC分类号： C12N5/0619 , C12N5/062 , C12N5/0621 , C12N2506/02
摘要： The present invention provides a clinically applicable method of inducing differentiation of embryonic stem cells, particularly a method of inducing differentiation of embryonic stem cells into forebrain neurons. More specifically, the present invention provides a method of inducing differentiation of embryonic stem cells, comprising culturing the embryonic stem cells as a floating aggregate in a serum-free medium, particularly a method of inducing differentiation of the embryonic stem cells into nervous system cells such as forebrain neurons and cerebellar neurons and sensory organ cells; a floating aggregate of embryonic stem cells obtained by culturing the embryonic stem cells as a floating aggregate in a serum-free medium; and cells derived from a floating aggregate of embryonic stem cells, particularly nervous system cells such as forebrain neurons and cerebellar neuron, sensory organ cells such as retinal precursor cells, and the like.
摘要翻译：本发明提供了诱导胚胎干细胞分化的临床适用方法，特别是诱导胚胎干细胞分化为前脑神经元的方法。更具体地，本发明提供了诱导胚胎干细胞分化的方法，包括将胚胎干细胞作为浮动聚集体培养在无血清培养基中，特别是诱导将胚胎干细胞分化为神经系统细胞的方法作为前脑神经元和小脑神经元和感觉器官细胞; 通过将胚胎干细胞作为浮动聚集体培养在无血清培养基中而获得的胚胎干细胞的浮动聚集体; 以及衍生自胚胎干细胞，特别是神经系统细胞如前脑神经元和小脑神经元，感觉器官细胞如视网膜前体细胞等的浮动聚集体的细胞。

8. 发明申请

US20130040330A1 METHOD FOR DIFFERENTIATION INDUCTION IN CULTURED STEM CELLS 审中-公开
标题翻译：培养干细胞分化诱导的方法
公开(公告)号：US20130040330A1
公开(公告)日：2013-02-14
申请号：US13508303
申请日：2010-11-05
申请人： Yoshiki Sasai , Mototsugu Eiraku
发明人： Yoshiki Sasai , Mototsugu Eiraku
IPC分类号： C12N5/0789 , C12Q1/02
摘要： Provided is a method of inducing the differentiation of a stem cell into nerve progenitor cells, comprising the step (1) of forming a homogenous aggregate of stem cells in a serum-free medium (1) and the step (2) of suspension-culturing the homogenous aggregate of stem cells in the presence of a basement membrane reference standard.
摘要翻译：提供了诱导干细胞分化成神经祖细胞的方法，包括在无血清培养基（1）和悬浮培养步骤（2）中形成干细胞的均匀聚集体的步骤（1）在存在基底膜参考标准的情况下干细胞的均匀聚集体。

9. 发明授权

US5679783A DNA encoding a tissue differentiation affecting factor 失效
标题翻译：编码组织分化影响因子的DNA
公开(公告)号：US5679783A
公开(公告)日：1997-10-21
申请号：US343760
申请日：1994-11-22
申请人： Edward M. De Robertis , Yoshiki Sasai
发明人： Edward M. De Robertis , Yoshiki Sasai
IPC分类号： A61K38/00 , C07K14/475 , C12N15/12 , C07K14/46
CPC分类号： C07K14/475 , A61K38/00 , C07K2319/00
摘要： A functional polypeptide designated "chordin" is described that is capable of inducing dorsal (and neural tissue) development in vertebrates, and which appears to be a secreted protein. There are substantial regions of conservation with Xenopus chordin with mouse chordin, and the human gene should also be similar in those regions. A full length Xenopus cDNA for chordin is illustrated by FIGS. 1A-1F, and contains a reading frame encoding a 941 residue, 105 kDa precursor protein.
摘要翻译：描述了能够在脊椎动物中诱导背部（和神经组织）发育，并且其似乎是分泌蛋白质的称为“chordin”的功能性多肽。有非常大的保护区域与爪蟾chordin与小鼠chordin，人类基因也应该是相似的那些地区。用于chordin的全长非洲爪蟾cDNA被图示。 1A-1F，并且包含编码941残基，105kDa前体蛋白的阅读框。

10. 发明申请

US20110091869A1 METHOD FOR CULTURE OF STEM CELL 审中-公开
标题翻译：干细胞培养方法
公开(公告)号：US20110091869A1
公开(公告)日：2011-04-21
申请号：US12996503
申请日：2009-06-05
申请人： Yoshiki Sasai , Takafumi Wataya , Mototsugu Eiraku
发明人： Yoshiki Sasai , Takafumi Wataya , Mototsugu Eiraku
IPC分类号： C12Q1/70 , C12N5/071 , C12N5/079 , C12N5/0797 , C12N5/0793 , C12Q1/68 , C12Q1/02
CPC分类号： C12N5/0623 , C12N2501/33 , C12N2501/415 , C12N2501/42 , C12N2501/70 , C12N2506/02
摘要： The present invention enables efficient suspension culture of stem cells in a serum-free medium by comprising a step for quickly forming a homogenous aggregate of stem cells, and provides a method of selectively inducing the differentiation of nerves from a stem cell, a method of forming a cerebral cortical nerve network in vitro, and a method of producing a steric structure of a brain tissue in vitro, as well as a method of producing hypothalamic neuron progenitor cells, comprising culturing pluripotent stem cells as a suspended aggregate in a serum-free medium that substantially does not contain a Nodal signal promoter, a Wnt signal promoter, an FGF signal promoter, a BMP signal promoter, retinoic acid and an insulin, and isolating hypothalamic neuron progenitor cells from the culture.
摘要翻译：本发明通过包括快速形成干细胞的均匀聚集体的步骤，能够有效地将干细胞悬浮培养在无血清培养基中，并且提供了选择性诱导神经从干细胞分化的方法，形成方法体外的脑皮质神经网络和体外产生脑组织的立体结构的方法，以及下丘脑神经元祖细胞的制造方法，包括将多能干细胞作为悬浮骨架培养于无血清培养基中基本上不含有Nodal信号启动子，Wnt信号启动子，FGF信号启动子，BMP信号启动子，视黄酸和胰岛素，并从培养物中分离下丘脑神经元祖细胞。

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