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1. 发明申请

US20070178521A1 Assay chip 审中-公开
标题翻译：测定芯片
公开(公告)号：US20070178521A1
公开(公告)日：2007-08-02
申请号：US10592748
申请日：2005-03-15
申请人： Yoshiki Sakaino , Yukio Sudo , Yoshihiko Abe , Bo Yang
发明人： Yoshiki Sakaino , Yukio Sudo , Yoshihiko Abe , Bo Yang
IPC分类号： G01N33/53 , C12M3/00
CPC分类号： B01L3/502753 , B01L3/5023 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0887 , B01L2400/0409
摘要： An integrated assay chip for assaying one or more components of a specimen, which comprises: (1) one or more pretreatment elements for pretreating a specimen; (2) one or more multilayer dry assay elements capable of assaying one or more components of the pretreated specimen; and (3) one or more flow channels connecting the pretreatment element and the multilayer dry assay element is provided.
摘要翻译：一种用于测定样品的一种或多种组分的综合测定芯片，其包括：（1）用于预处理样品的一种或多种预处理元件; （2）能够测定预处理样品的一种或多种组分的一种或多种多层干测定元件; 和（3）提供连接预处理元件和多层干测定元件的一个或多个流动通道。

2. 发明申请

US20070178009A1 Analysis element for use in method of testing specimen 审中-公开
标题翻译：分析元素用于测试样品的方法
公开(公告)号：US20070178009A1
公开(公告)日：2007-08-02
申请号：US10592230
申请日：2005-03-15
申请人： Yoshiki Sakaino , Yoshihiko Abe , Yukio Sudo
发明人： Yoshiki Sakaino , Yoshihiko Abe , Yukio Sudo
IPC分类号： G01N21/00
CPC分类号： A61B5/157 , A61B5/14532 , A61B5/14546 , A61B5/1455 , A61B5/1486 , A61B5/150022 , A61B5/150099 , A61B5/150274 , A61B5/150343 , A61B5/150358 , A61B5/150389 , A61B2560/0223 , A61B2562/0295 , G01N21/31 , G01N21/4738 , G01N21/75 , G01N31/22
摘要： An analysis element for use in a blood test method enabling easy and simple operations that are performed quickly up to measurement, an analysis element for use in a blood test method that operations up to the measurement for many components can be quickly performed, and that is safe and has a sufficient measurement accuracy thereof, and an analysis element for use in a test method using body fluids and urines of humans and animals, plain water, seawater, soil extract, agricultural products, marine products, processed-food extracts, and liquid for use in scientific research as specimens, are provided, the present invention relates to a multi-component measurement dry analysis element for use in a method for testing a specimen, the method using an area sensor as a detector to obtain a result of measurement according to information represented by 1000 pixels or more per one component, and to perform simultaneous measurements of plural components.
摘要翻译：一种用于血液检查方法的分析元件，其能够实现快速进行测量的简单且简单的操作，用于血液测试方法的分析元件可以快速执行多达成分测量的操作，也就是说安全且具有足够的测量精度，以及用于使用人体和动物，普通水，海水，土壤提取物，农产品，海产品，加工食品提取物和液体的体液和尿液的测试方法中的分析元件本发明涉及一种用于试样检测方法的多组分测量干燥分析元件，该方法使用区域传感器作为检测器，以获得测量结果对于每个分量由1000个像素或更多个表示的信息，并且执行多个分量的同时测量。

3. 发明申请

US20060068491A1 Microdevice for performing method of separating and purifying nucleic acid 审中-公开
标题翻译：用于分离和纯化核酸的微型设备
公开(公告)号：US20060068491A1
公开(公告)日：2006-03-30
申请号：US11227245
申请日：2005-09-16
申请人： Yoshihiko Makino , Yoshiki Sakaino , Yukio Sudo , Yoshihiko Abe
发明人： Yoshihiko Makino , Yoshiki Sakaino , Yukio Sudo , Yoshihiko Abe
IPC分类号： C12M1/34 , C07H21/04
CPC分类号： B01L3/502753 , B01L3/502707 , B01L3/502715 , B01L2200/0631 , B01L2300/0816 , B01L2300/0867 , B01L2400/0406 , B01L2400/0418 , B01L2400/0487
摘要： A microdevice for performing a method for separating and purifying a nucleic acid, the microdevice comprising: at least one opening; and at least one channel for passing a sample solution, wherein the method comprises: (A) a step of bringing a nucleic acid-containing sample solution into contact with a nucleic acid-adsorbing support having a function of adsorbing a nucleic acid; (B) a step of washing the nucleic acid-adsorbing support with a washing solution in a state of a nucleic acid being adsorbed to the support; and (C) a step of desorbing the nucleic acid from the nucleic acid-adsorbing support by a recovering solution, thereby purifying the nucleic acid; an apparatus for utilizing the microdevice; and a reagent kit for use in the microdevice.
摘要翻译：一种用于进行分离和纯化核酸的方法的微型装置，所述微型装置包括：至少一个开口; 和用于通过样品溶液的至少一个通道，其中所述方法包括：（A）使含核酸样品溶液与具有吸附核酸功能的核酸吸附载体接触的步骤; （B）在被吸附在所述载体上的核酸的状态下用洗涤液洗涤所述核酸吸附载体的工序; 和（C）通过回收溶液从核酸吸附载体解吸核酸的步骤，从而纯化核酸; 一种利用微型装置的装置; 和用于微型装置的试剂盒。

4. 发明申请

US20050202569A1 Integrated microchemical device 审中-公开
标题翻译：集成微化学装置
公开(公告)号：US20050202569A1
公开(公告)日：2005-09-15
申请号：US11075434
申请日：2005-03-09
申请人： Yoshiki Sakaino , Yukio Sudo , Yoshihiko Abe
发明人： Yoshiki Sakaino , Yukio Sudo , Yoshihiko Abe
IPC分类号： G01N31/20 , G01N33/00 , G01N33/52 , G01N37/00
CPC分类号： G01N33/525
摘要： A microchemical device capable of promoting mixing of a specimen and a reagent particularly in analyzing trace constituents of liquid such as blood or body fluid, and a microchemical device that can rapidly and easily analyze one or more constituents from a small amount of specimen, are provided, which is an integrated microchemical device for analyzing one or more constituents of a specimen, the microchemical device comprising at least the two elements of (a) one or more flow channels through which a specimen passes and (b) a multilayer dry analysis element that can come in contact with at least one of the flow channels and analyze the constituents of the specimen.
摘要翻译：提供能够促进样品和试剂混合的微化学装置，特别是在分析液体，如血液或体液的微量成分的微量化学装置中，以及能够从少量样品快速，容易地分析一种或多种成分的微化学装置，其是用于分析样品的一种或多种成分的集成微量化学装置，所述微化学装置至少包括（a）样品通过的一个或多个流动通道的两个元件和（b）多层干燥分析元件，可以与至少一个流动通道接触并分析试样的组分。

5. 发明授权

US07846716B2 Microchip and analysis method using the same 有权
标题翻译： Microchip及其分析方法使用
公开(公告)号：US07846716B2
公开(公告)日：2010-12-07
申请号：US11413138
申请日：2006-04-28
申请人： Bo Yang , Yukio Sudo , Yoshiki Sakaino , Hideyuki Karaki , Akira Wakabayashi
发明人： Bo Yang , Yukio Sudo , Yoshiki Sakaino , Hideyuki Karaki , Akira Wakabayashi
IPC分类号： C12M1/36
CPC分类号： B01L3/5027 , B01L2200/0605 , B01L2200/0642 , B01L2200/16 , B01L2300/0609 , B01L2300/0636 , B01L2300/0645 , B01L2300/0825 , B01L2300/087 , B01L2300/161 , B01L2400/0406 , G01N33/54366 , G01N33/54386 , G01N2035/00217
摘要： An object of the present invention is to provide a microchip whereby, when chemical analysis is conducted, a specimen liquid can be directly collected and weighed without using any collecting and weighing devices. The present invention provides a microchip for analyzing liquid samples, which has a measuring structure for weighing and collecting a given amount of a specimen liquid within a range of 0.05 to 10 μl from an excessive amount of the specimen liquid which was introduced in the chip, wherein the measuring structure is located at the upstream side of an analysis element for analyzing a target substance in the specimen liquid inside the microchip.
摘要翻译：本发明的目的是提供一种微芯片，其中，当进行化学分析时，可以直接收集和称重样品液体，而不使用任何收集和称重装置。本发明提供了一种用于分析液体样品的微芯片，其具有用于从引入到芯片中的过量的样本液体对0.05〜10μl的范围内的给定量的标本液体进行称重和收集的测量结构，其中所述测量结构位于用于分析所述微芯片内的所述样本液体中的目标物质的分析元件的上游侧。

6. 发明申请

US20060263242A1 Microchip and analysis method using the same 有权
标题翻译： Microchip及其分析方法使用
公开(公告)号：US20060263242A1
公开(公告)日：2006-11-23
申请号：US11413138
申请日：2006-04-28
申请人： Bo Yang , Yukio Sudo , Yoshiki Sakaino , Hideyuki Karaki , Akira Wakabayashi
发明人： Bo Yang , Yukio Sudo , Yoshiki Sakaino , Hideyuki Karaki , Akira Wakabayashi
IPC分类号： G01N31/22
CPC分类号： B01L3/5027 , B01L2200/0605 , B01L2200/0642 , B01L2200/16 , B01L2300/0609 , B01L2300/0636 , B01L2300/0645 , B01L2300/0825 , B01L2300/087 , B01L2300/161 , B01L2400/0406 , G01N33/54366 , G01N33/54386 , G01N2035/00217
摘要： An object of the present invention is to provide a microchip whereby, when chemical analysis is conducted, a specimen liquid can be directly collected and weighed without using any collecting and weighing devices. The present invention provides a microchip for analyzing liquid samples, which has a measuring structure for weighing and collecting a given amount of a specimen liquid within a range of 0.05 to 10 μl from an an excessive amount of the specimen liquid which was introduced in the chip, wherein the measuring structure is located at the upstream side of an analysis element for analyzing a target substance in the specimen liquid inside the microchip.
摘要翻译：本发明的目的是提供一种微芯片，其中，当进行化学分析时，可以直接收集和称重样品液体，而不使用任何收集和称重装置。本发明提供了一种用于分析液体样品的微芯片，该微芯片具有用于从引入到芯片中的过量的样本液体，在0.05至10微米的范围内对给定量的标本液体进行称重和收集的测量结构其中，所述测量结构位于用于分析所述微芯片内的所述样本液体中的目标物质的分析元件的上游侧。

7. 发明授权

US07407578B2 Liquid filtering instrument and dry type analysis device 失效
标题翻译：液体过滤仪和干式分析仪
公开(公告)号：US07407578B2
公开(公告)日：2008-08-05
申请号：US11185822
申请日：2005-07-21
申请人： Yoshiki Sakaino , Yukio Sudo
发明人： Yoshiki Sakaino , Yukio Sudo
IPC分类号： B01D29/00
CPC分类号： B01D39/2017 , B01D2239/0492 , G01N33/491
摘要： A liquid filtering instrument 10 according to the present invention achieves filtered liquid by pressure-reduced filtration, and it comprises a filter member 12 in which a liquid filter 15 is accommodated, and a holder member 14 for stocking filtered liquid passed through the liquid filter 15, wherein the filter member 12 and the holder member 14 are formed in capable of being fitted so as to be kept substantially air-tight and water-tight under pressure reduced state.
摘要翻译：根据本发明的液体过滤装置10通过减压过滤实现过滤液体，并且其包括其中容纳有液体过滤器15的过滤构件12和用于储存通过液体过滤器15的过滤液体的保持构件14 ，其中过滤构件12和保持器构件14形成为能够被装配成在压力降低状态下基本上保持气密性和水密性。

8. 发明申请

US20060018798A1 Liquid filtering instrument and dry type analysis device 失效
标题翻译：液体过滤仪和干式分析仪
公开(公告)号：US20060018798A1
公开(公告)日：2006-01-26
申请号：US11185822
申请日：2005-07-21
申请人： Yoshiki Sakaino , Yukio Sudo
发明人： Yoshiki Sakaino , Yukio Sudo
IPC分类号： B01L11/00
CPC分类号： B01D39/2017 , B01D2239/0492 , G01N33/491
摘要： A liquid filtering instrument 10 according to the present invention achieves filtered liquid by pressure-reduced filtration, and it comprises a filter member 12 in which a liquid filter 15 is accommodated, and a holder member 14 for stocking filtered liquid passed through the liquid filter 15, wherein the filter member 12 and the holder member 14 are formed in capable of being fitted so as to be kept substantially air-tight and water-tight under pressure reduced state.
摘要翻译：根据本发明的液体过滤装置10通过减压过滤实现过滤液体，并且其包括其中容纳有液体过滤器15的过滤构件12和用于储存通过液体过滤器15的过滤液体的保持构件14 ，其中过滤构件12和保持器构件14形成为能够被装配成在压力降低状态下基本上保持气密性和水密性。

9. 发明申请

US20070141616A1 Microreactor 审中-公开
标题翻译：微反应器
公开(公告)号：US20070141616A1
公开(公告)日：2007-06-21
申请号：US11657538
申请日：2007-01-25
申请人： Yukio Sudo
发明人： Yukio Sudo
IPC分类号： C12Q1/68 , G01N33/53 , C12M1/34
CPC分类号： G01N33/558
摘要： The present invention provides a reactive microreactor wherein a group of the following formula (1) which can bind to a member of specifically binding partner, is bound to a part or entirety of a wall surface of a channel: -L-SO2—X1 (1) wherein X1 represents —CR1═CR2R3 or —CHR1—CR2R3Y; R1, R2 and R3 independently represent an atom or a group selected from the group consisting of a hydrogen atom, a C1-6 alkyl group, a C6-20 aryl group, and a C7-20 aralkyl group having a C1-6 alkyl chain; Y represents a group which can be substitutable by a nucleophilic reagent or a group which is released as “HY” by a base; and L represents a linking group. The microreactor according to the present invention has advantages that it requires a less amount of a reagent and achieves a higher S/N ratio.
摘要翻译：本发明提供了一种反应性微反应器，其中可结合特异性结合配偶体成员的下列通式（1）的基团与通道的壁表面的一部分或全部结合：描述=“在线公式”end =“lead”？> - L-SO <2> <1> <1> <？in-line-formula description =“ 在线公式“end =”tail“？>其中X 1表示-CR 1 -CR 2 2 R 3， SUP>或-CHR 1 -CR 2 R 3 3 Y; R 1，R 2和R 3独立地表示选自以下的原子或基团：氢原子，C 1-6 烷基，C6-20芳基和具有C1-6烷基链的C7-20芳烷基; Y表示可以被亲核试剂或被基团释放为“HY”的基团取代的基团; L表示连接基团。根据本发明的微反应器具有需要较少量的试剂并实现更高S / N比的优点。

10. 发明授权

US07109314B2 Fluorescent nucleotides 有权
公开(公告)号：US07109314B2
公开(公告)日：2006-09-19
申请号：US09829467
申请日：2001-04-09
申请人： Hiroshi Shinoki , Hiroko Inomata , Masayoshi Kojima , Yukio Sudo , Osamu Seshimoto
发明人： Hiroshi Shinoki , Hiroko Inomata , Masayoshi Kojima , Yukio Sudo , Osamu Seshimoto
IPC分类号： C07H21/02 , C12Q1/68 , A61N57/00
CPC分类号： C07H21/00
摘要： According to the present invention, there is provided a fluorescent nucleotide represented by the formula: A-B-C,wherein A represents a residue of natural or synthetic nucleotide, oligonucleotide, polynucleotide, or derivative thereof, and binds to B at a base moiety in said residue; B represents a divalent linking group or a single bond; and C represents a monovalent group derived from a fluorescent dye having 0 or 1 sulfonic acid group or phosphoric acid group in a molecule. The present invention provides useful fluorescent nucleotides for labeling nucleic acids, specifically, fluorescent nucleotides of which uptake ratio is high in synthetic reaction of nucleic acids.

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