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1. 发明申请

US20060068491A1 Microdevice for performing method of separating and purifying nucleic acid 审中-公开
标题翻译：用于分离和纯化核酸的微型设备
公开(公告)号：US20060068491A1
公开(公告)日：2006-03-30
申请号：US11227245
申请日：2005-09-16
申请人： Yoshihiko Makino , Yoshiki Sakaino , Yukio Sudo , Yoshihiko Abe
发明人： Yoshihiko Makino , Yoshiki Sakaino , Yukio Sudo , Yoshihiko Abe
IPC分类号： C12M1/34 , C07H21/04
CPC分类号： B01L3/502753 , B01L3/502707 , B01L3/502715 , B01L2200/0631 , B01L2300/0816 , B01L2300/0867 , B01L2400/0406 , B01L2400/0418 , B01L2400/0487
摘要： A microdevice for performing a method for separating and purifying a nucleic acid, the microdevice comprising: at least one opening; and at least one channel for passing a sample solution, wherein the method comprises: (A) a step of bringing a nucleic acid-containing sample solution into contact with a nucleic acid-adsorbing support having a function of adsorbing a nucleic acid; (B) a step of washing the nucleic acid-adsorbing support with a washing solution in a state of a nucleic acid being adsorbed to the support; and (C) a step of desorbing the nucleic acid from the nucleic acid-adsorbing support by a recovering solution, thereby purifying the nucleic acid; an apparatus for utilizing the microdevice; and a reagent kit for use in the microdevice.
摘要翻译：一种用于进行分离和纯化核酸的方法的微型装置，所述微型装置包括：至少一个开口; 和用于通过样品溶液的至少一个通道，其中所述方法包括：（A）使含核酸样品溶液与具有吸附核酸功能的核酸吸附载体接触的步骤; （B）在被吸附在所述载体上的核酸的状态下用洗涤液洗涤所述核酸吸附载体的工序; 和（C）通过回收溶液从核酸吸附载体解吸核酸的步骤，从而纯化核酸; 一种利用微型装置的装置; 和用于微型装置的试剂盒。

2. 发明申请

US20070178009A1 Analysis element for use in method of testing specimen 审中-公开
标题翻译：分析元素用于测试样品的方法
公开(公告)号：US20070178009A1
公开(公告)日：2007-08-02
申请号：US10592230
申请日：2005-03-15
申请人： Yoshiki Sakaino , Yoshihiko Abe , Yukio Sudo
发明人： Yoshiki Sakaino , Yoshihiko Abe , Yukio Sudo
IPC分类号： G01N21/00
CPC分类号： A61B5/157 , A61B5/14532 , A61B5/14546 , A61B5/1455 , A61B5/1486 , A61B5/150022 , A61B5/150099 , A61B5/150274 , A61B5/150343 , A61B5/150358 , A61B5/150389 , A61B2560/0223 , A61B2562/0295 , G01N21/31 , G01N21/4738 , G01N21/75 , G01N31/22
摘要： An analysis element for use in a blood test method enabling easy and simple operations that are performed quickly up to measurement, an analysis element for use in a blood test method that operations up to the measurement for many components can be quickly performed, and that is safe and has a sufficient measurement accuracy thereof, and an analysis element for use in a test method using body fluids and urines of humans and animals, plain water, seawater, soil extract, agricultural products, marine products, processed-food extracts, and liquid for use in scientific research as specimens, are provided, the present invention relates to a multi-component measurement dry analysis element for use in a method for testing a specimen, the method using an area sensor as a detector to obtain a result of measurement according to information represented by 1000 pixels or more per one component, and to perform simultaneous measurements of plural components.
摘要翻译：一种用于血液检查方法的分析元件，其能够实现快速进行测量的简单且简单的操作，用于血液测试方法的分析元件可以快速执行多达成分测量的操作，也就是说安全且具有足够的测量精度，以及用于使用人体和动物，普通水，海水，土壤提取物，农产品，海产品，加工食品提取物和液体的体液和尿液的测试方法中的分析元件本发明涉及一种用于试样检测方法的多组分测量干燥分析元件，该方法使用区域传感器作为检测器，以获得测量结果对于每个分量由1000个像素或更多个表示的信息，并且执行多个分量的同时测量。

3. 发明申请

US20070178521A1 Assay chip 审中-公开
标题翻译：测定芯片
公开(公告)号：US20070178521A1
公开(公告)日：2007-08-02
申请号：US10592748
申请日：2005-03-15
申请人： Yoshiki Sakaino , Yukio Sudo , Yoshihiko Abe , Bo Yang
发明人： Yoshiki Sakaino , Yukio Sudo , Yoshihiko Abe , Bo Yang
IPC分类号： G01N33/53 , C12M3/00
CPC分类号： B01L3/502753 , B01L3/5023 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0887 , B01L2400/0409
摘要： An integrated assay chip for assaying one or more components of a specimen, which comprises: (1) one or more pretreatment elements for pretreating a specimen; (2) one or more multilayer dry assay elements capable of assaying one or more components of the pretreated specimen; and (3) one or more flow channels connecting the pretreatment element and the multilayer dry assay element is provided.
摘要翻译：一种用于测定样品的一种或多种组分的综合测定芯片，其包括：（1）用于预处理样品的一种或多种预处理元件; （2）能够测定预处理样品的一种或多种组分的一种或多种多层干测定元件; 和（3）提供连接预处理元件和多层干测定元件的一个或多个流动通道。

4. 发明申请

US20050202569A1 Integrated microchemical device 审中-公开
标题翻译：集成微化学装置
公开(公告)号：US20050202569A1
公开(公告)日：2005-09-15
申请号：US11075434
申请日：2005-03-09
申请人： Yoshiki Sakaino , Yukio Sudo , Yoshihiko Abe
发明人： Yoshiki Sakaino , Yukio Sudo , Yoshihiko Abe
IPC分类号： G01N31/20 , G01N33/00 , G01N33/52 , G01N37/00
CPC分类号： G01N33/525
摘要： A microchemical device capable of promoting mixing of a specimen and a reagent particularly in analyzing trace constituents of liquid such as blood or body fluid, and a microchemical device that can rapidly and easily analyze one or more constituents from a small amount of specimen, are provided, which is an integrated microchemical device for analyzing one or more constituents of a specimen, the microchemical device comprising at least the two elements of (a) one or more flow channels through which a specimen passes and (b) a multilayer dry analysis element that can come in contact with at least one of the flow channels and analyze the constituents of the specimen.
摘要翻译：提供能够促进样品和试剂混合的微化学装置，特别是在分析液体，如血液或体液的微量成分的微量化学装置中，以及能够从少量样品快速，容易地分析一种或多种成分的微化学装置，其是用于分析样品的一种或多种成分的集成微量化学装置，所述微化学装置至少包括（a）样品通过的一个或多个流动通道的两个元件和（b）多层干燥分析元件，可以与至少一个流动通道接触并分析试样的组分。

5. 发明申请

US20050002038A1 Optical measuring apparatus and optical measuring method 失效
标题翻译：光学测量仪器和光学测量方法
公开(公告)号：US20050002038A1
公开(公告)日：2005-01-06
申请号：US10849777
申请日：2004-05-21
申请人： Yoshiki Sakaino , Yoshihiko Abe , Kaoru Terashima
发明人： Yoshiki Sakaino , Yoshihiko Abe , Kaoru Terashima
IPC分类号： G01N21/01 , G01N21/27 , G01N21/31 , G01N21/47 , G01N21/59 , G01N21/86
CPC分类号： G01N21/8483 , G01N21/31 , G01N21/4738
摘要： An optical measuring apparatus comprises: an illuminating section that illuminates a first object with a first light; a light changing section that changes an intensity of the first light illuminating the first object; a light receiving device that receives a second light transmitted through or reflected from the first object; and an output section that outputs a measurement result according to i) a state of the light changing section and ii) an amount of the second light received by the light receiving device. In addition, an optical measuring apparatus comprises: the illuminating section; the light receiving device; a light receiving time changing section that changes a light receiving time of the light receiving device; and an output section that outputs a measurement result according to i) the light receiving time and ii) an amount of the second light received by the light receiving device.
摘要翻译：光学测量装置包括：用第一光照亮第一物体的照明部分; 光改变部，其改变照射第一物体的第一光的强度; 光接收装置，其接收透过或者从第一物体反射的第二光; 以及输出部，其根据i）光改变部的状态输出测量结果，以及ii）由光接收装置接收的第二光的量。另外，光学测量装置包括：照明部; 光接收装置; 光接收时间改变部，其改变所述光接收装置的光接收时间; 以及输出部，其根据i）光接收时间输出测量结果，以及ii）由光接收装置接收的第二光的量。

6. 发明授权

US07369243B2 Optical measuring apparatus and optical measuring method 失效
标题翻译：光学测量仪器和光学测量方法
公开(公告)号：US07369243B2
公开(公告)日：2008-05-06
申请号：US10849777
申请日：2004-05-21
申请人： Yoshiki Sakaino , Yoshihiko Abe , Kaoru Terashima
发明人： Yoshiki Sakaino , Yoshihiko Abe , Kaoru Terashima
IPC分类号： G01N21/47
CPC分类号： G01N21/8483 , G01N21/31 , G01N21/4738
摘要： An optical measuring apparatus comprises: an illuminating section that illuminates a first object with a first light; a light changing section that changes an intensity of the first light illuminating the first object; a light receiving device that receives a second light transmitted through or reflected from the first object; and an output section that outputs a measurement result according to i) a state of the light changing section and ii) an amount of the second light received by the light receiving device. In addition, an optical measuring apparatus comprises: the illuminating section; the light receiving device; a light receiving time changing section that changes a light receiving time of the light receiving device; and an output section that outputs a measurement result according to i) the light receiving time and ii) an amount of the second light received by the light receiving device.
摘要翻译：光学测量装置包括：用第一光照亮第一物体的照明部分; 光改变部，其改变照射第一物体的第一光的强度; 光接收装置，其接收透过或者从第一物体反射的第二光; 以及输出部，其根据i）光改变部的状态输出测量结果，以及ii）由光接收装置接收的第二光的量。另外，光学测量装置包括：照明部; 光接收装置; 光接收时间改变部，其改变所述光接收装置的光接收时间; 以及输出部，其根据i）光接收时间输出测量结果，以及ii）由光接收装置接收的第二光的量。

7. 发明申请

US20070219509A1 Blood collecting needle, syringe needle, winged needle, test kit and blood collecting kit 审中-公开
标题翻译：采血针，注射器针，翼针，检测试剂盒和采血试剂盒
公开(公告)号：US20070219509A1
公开(公告)日：2007-09-20
申请号：US11717689
申请日：2007-03-14
申请人： Tomoko Tashiro , Yoshiki Sakaino , Yoshihiko Abe
发明人： Tomoko Tashiro , Yoshiki Sakaino , Yoshihiko Abe
IPC分类号： A61M5/32
CPC分类号： A61B5/1545 , A61B5/15003 , A61B5/150396 , A61B5/150473 , A61B5/150572 , A61B5/150717 , A61B5/150732 , A61B5/15074
摘要： A blood collecting needle, which comprises: a needle tube having a human-body puncturing needle tube and a stopper-body puncturing needle tube, wherein the needle tube has an outer diameter having substantially the same dimension from a tip of the human-body puncturing needle tube to a tip of the stopper-body puncturing needle tube, the needle tube has an inner diameter having substantially the same dimension from the tip of the human-body puncturing needle tube to the tip of the stopper-body puncturing needle tube, and the blood collecting needle satisfies equation (1): 0.35≦D1
摘要翻译：一种采血针，其特征在于，包括：针管，其具有人体穿刺针管和塞体穿刺针管，其中，所述针管的外径与所述人体穿刺的前端具有大致相同的尺寸针管到塞体穿刺针管的尖端，针管具有从人体穿刺针管的尖端到塞体穿刺针管的前端具有大致相同尺寸的内径，以及采血针满足式（1）：<？in-line-formula description =“In-line formula”end =“lead”？> 0.35 <= D <1> <0.70（等式1） <？in-line-formula description =“In-line formula”end =“tail”？>其中D <1>表示针管的外径（mm）。

8. 发明申请

US20070149944A1 Blood collecting needle 审中-公开
标题翻译：采血针
公开(公告)号：US20070149944A1
公开(公告)日：2007-06-28
申请号：US11634839
申请日：2006-12-07
申请人： Tomoko Tashiro , Yoshiki Sakaino , Yoshihiko Abe
发明人： Tomoko Tashiro , Yoshiki Sakaino , Yoshihiko Abe
IPC分类号： A61M5/32
CPC分类号： A61B5/150488 , A61B5/15003 , A61B5/150282 , A61B5/150396 , A61B5/150572 , A61B5/150717 , A61B5/150732 , A61B5/1545 , A61M5/3286 , A61M5/329
摘要： A blood collecting needle, which comprises: a needle hub; a human-body puncturing needle tube provided at one side of the needle hub; and a plug-body puncturing needle tube provided at the other side of the needle hub, wherein an outer diameter of a tip of the human-body puncturing needle tube and an outer diameter of a tip of the plug-body puncturing needle tube are different from each other.
摘要翻译：一种采血针，其特征在于，包括：针座; 设置在针座的一侧的人体穿刺针管; 以及设置在针座的另一侧的插头体穿刺针管，其中，人体穿刺针管的前端的外径和插塞体穿刺针管的前端的外径不同从彼此。

9. 发明授权

US5972635A Sulfonic acid group buffered dry analytical element for quantitative analysis of creatine kinase or its MB isozyme 有权
标题翻译：磺酸基缓冲干燥分析元素用于定量分析肌酸激酶或其MB同工酶
公开(公告)号：US5972635A
公开(公告)日：1999-10-26
申请号：US158485
申请日：1998-09-22
申请人： Hideaki Tanaka , Yoshihiko Abe , Yoshiki Sakaino , Kaoru Terashima
发明人： Hideaki Tanaka , Yoshihiko Abe , Yoshiki Sakaino , Kaoru Terashima
IPC分类号： C12Q1/32 , C12Q1/48 , C12Q1/50 , G01N33/52 , G01N33/70
CPC分类号： G01N33/70 , C12Q1/50
摘要： A dry analytical element for quantitatively analyzing creatine kinase is composed of a support and a creatine kinase-detecting agent layer. The creatine kinase-detecting agent layer contains creatine phosphate and adenosine diphosphate which react with each other to give adenosine triphosphate in the presence of creatine kinase, a buffer compound having a sulfonic acid group which keeps the layer in the range of pH 5.5 to pH 8.5 for the formation of adenosine triphosphate, and an indicator composition which reacts with the formed adenosine triphosphate to give a spectroscopically detectable compound. An analytical element for analysis of creatine kinase MB isozyme further contains in the layer an antibody which specifically inhibits activity of M sub-unit of creatine kinase.
摘要翻译：用于定量分析肌酸激酶的干燥分析元件由载体和肌酸激酶检测剂层组成。肌酸激酶检测剂层含有磷酸肌酸和二磷酸腺苷，其在肌酸激酶存在下相互反应以产生腺苷三磷酸，其是具有磺酸基的缓冲化合物，其将该层保持在pH5.5至pH8.5的范围内用于形成三磷酸腺苷，以及与形成的三磷酸腺苷反应以产生光谱可检测化合物的指示剂组合物。用于分析肌酸激酶MB同功酶的分析元素在该层中进一步含有特异性抑制肌酸激酶M亚单位活性的抗体。

10. 发明授权

US06399305B1 Protection of partial complementary nucleic acid fragment using a electroconductive chip and intercalator 有权
标题翻译：使用导电芯片和嵌入剂保护部分互补核酸片段
公开(公告)号：US06399305B1
公开(公告)日：2002-06-04
申请号：US09588950
申请日：2000-06-07
申请人： Yoshihiko Makino , Yoshihiko Abe , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita , Masashi Ogawa
发明人： Yoshihiko Makino , Yoshihiko Abe , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita , Masashi Ogawa
IPC分类号： C12Q168
CPC分类号： C12Q1/6825 , Y10T436/143333 , C12Q2563/173 , C12Q2563/113
摘要： A method of analyzing a nucleic acid fragment sample to judge whether the nucleic acid fragment sample is uncomplementary, partly complementary or complementary to a DNA fragment in its specific base sequence is conducted by the steps of: bringing an aqueous solution of the nucleic acid fragment sample into contact with a DNA chip having an electroconductive substrate and the DNA fragment fixed onto the substrate in the presence of an electrochemical thread intercalator; measuring an electric current flowing from or to the electroconductive substrate along the DNA fragment under application of a potential to the substrate; and comparing the electric current measured above with a referential electric current which is prepared employing a DNA chip equivalent to the above DNA chip, the intercalator, and an aqueous solution of a nucleic acid fragment which is complementary to the DNA fragment of the DNA chip.
摘要翻译：分析核酸片段样品以判断核酸片段样品是否与其特异性碱基序列中的DNA片段不互补，部分互补或互补的方法通过以下步骤进行：使核酸片段样品的水溶液与具有导电性基体的DNA芯片接触，并且在电化学线性嵌入剂的存在下固定在基材上的DNA片段;测量在施加电位下沿着DNA片段向基片流向或流到导电基底的电流 ; 并使用与上述DNA芯片相当的DNA芯片，嵌入剂和与DNA芯片的DNA片段互补的核酸片段的水溶液制备的参考电流来比较上述电流。

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