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1. 发明申请

US20100000880A1 BIOSENSOR MEASUREMENT SYSTEM AND MEASUREMENT METHOD 有权
标题翻译：生物传感器测量系统和测量方法
公开(公告)号：US20100000880A1
公开(公告)日：2010-01-07
申请号：US12375111
申请日：2007-07-26
申请人： Yoshihiro Itoh , Eriko Yamanishi , Tsuyoshi Takahashi , Hiroyuki Tokunaga
发明人： Yoshihiro Itoh , Eriko Yamanishi , Tsuyoshi Takahashi , Hiroyuki Tokunaga
IPC分类号： C12Q1/25 , G01N27/26
CPC分类号： G01N27/3274
摘要： There is provided a biosensor measurement system which can output a highly-precise measurement result even when an impact such as falling of the sensor occurs or the biosensor is an exposed sensor. An abnormal waveform detection electrode is provided in addition to electrodes for quantitative determination of a target substance. Therefore, when an impact is caused by such as falling of the sensor in a halt period where no voltage is applied in a voltage application algorithm, the abnormal waveform detection electrode can detect the impact. Further, also an exposed sensor can be detected by the abnormal waveform which is detected by the abnormal waveform detection electrode.
摘要翻译：提供了一种生物传感器测量系统，即使当传感器出现下降或生物传感器是暴露的传感器时也能输出高精度的测量结果。除了用于定量目标物质的电极之外，还提供异常波形检测电极。因此，在电压施加算法中，如果在不施加电压的停止期间传感器的下降引起冲击，则异常波形检测电极可以检测出影响。此外，也可以通过由异常波形检测电极检测到的异常波形来检测曝光的传感器。

2. 发明授权

US08945369B2 Biosensor measurement system and measurement method 有权
标题翻译：生物传感器测量系统和测量方法
公开(公告)号：US08945369B2
公开(公告)日：2015-02-03
申请号：US12375111
申请日：2007-07-26
申请人： Yoshihiro Itoh , Eriko Yamanishi , Tsuyoshi Takahashi , Hiroyuki Tokunaga
发明人： Yoshihiro Itoh , Eriko Yamanishi , Tsuyoshi Takahashi , Hiroyuki Tokunaga
IPC分类号： C12Q1/25 , G01N27/26 , G01N27/327
CPC分类号： G01N27/3274
摘要： There is provided a biosensor measurement system which can output a highly-precise measurement result even when an impact such as falling of the sensor occurs or the biosensor is an exposed sensor. An abnormal waveform detection electrode is provided in addition to electrodes for quantitative determination of a target substance. Therefore, when an impact is caused by such as falling of the sensor in a halt period where no voltage is applied in a voltage application algorithm, the abnormal waveform detection electrode can detect the impact. Further, also an exposed sensor can be detected by the abnormal waveform which is detected by the abnormal waveform detection electrode.
摘要翻译：提供了一种生物传感器测量系统，即使当传感器出现下降或生物传感器是暴露的传感器时也能输出高精度的测量结果。除了用于定量目标物质的电极之外，还提供异常波形检测电极。因此，在电压施加算法中，如果在不施加电压的停止期间传感器的下降引起冲击，则异常波形检测电极可以检测出影响。此外，也可以通过由异常波形检测电极检测到的异常波形来检测曝光的传感器。

3. 发明申请

US20120251708A1 BIOSENSOR, THIN FILM ELECTRODE FORMING METHOD, QUANTIFICATION APPARATUS, AND QUANTIFICATION METHOD 有权
标题翻译：生物传感器，薄膜电极形成方法，量化装置和定量方法
公开(公告)号：US20120251708A1
公开(公告)日：2012-10-04
申请号：US13490553
申请日：2012-06-07
申请人： Shoji Miyazaki , Hiroyuki Tokunaga , Masaki Fujiwara , Eriko Yamanishi , Yoshinobu Tokuno
发明人： Shoji Miyazaki , Hiroyuki Tokunaga , Masaki Fujiwara , Eriko Yamanishi , Yoshinobu Tokuno
IPC分类号： H05K13/04 , B05D5/12 , B23K26/38 , C23C14/34
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.
摘要翻译：公开了一种生物传感器，其包括支撑件; 由诸如金或钯等贵金属的导电材料和碳组成的导电层; 平行于和垂直于支撑体侧面的狭缝; 工作，计数器和检测电极; 覆盖支架上的工作电极，反电极和检测电极的间隔件; 在间隔件中形成矩形切口，形成试样供应路径; 样品供应路径的入口; 通过将含有酶的试剂施加到通过所述间隔件中的切口暴露的所述工作，反向和检测电极而形成的试剂层; 和间隔物上的盖子。可以通过简单的方法形成生物传感器，并且不管试剂组成如何，在电极上提供均匀的试剂层。

4. 发明申请

US20110278167A1 Biosensor, thin film electrode forming method, quantification apparatus, and quantification method 有权
标题翻译：生物传感器，薄膜电极形成方法，定量装置和定量方法
公开(公告)号：US20110278167A1
公开(公告)日：2011-11-17
申请号：US13135848
申请日：2011-07-15
申请人： Shoji Miyazaki , Hiroyuki Tokunaga , Masaki Fujiwara , Eriko Yamanishi , Yoshinobu Tokuno
发明人： Shoji Miyazaki , Hiroyuki Tokunaga , Masaki Fujiwara , Eriko Yamanishi , Yoshinobu Tokuno
IPC分类号： G01N27/327
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor according to the present invention comprises a support 1, a conductive layer 2 composed of an electrical conductive material such as noble metal, for example gold, palladium or the like, and carbon, slits 3a and 3b parallel to the side of the support 1, slits 4a and 4b vertical to the side of the support 1, a working electrode 5, a counter electrode 6, a detecting electrode 7, a spacer 8 which covers the working electrode 5, the counter electrode 6 and the detecting electrode 7 on the support 1, a rectangular cutout part 9 forming a specimen supply path, an inlet 9a of the specimen supply path, a reagent layer 12 formed by applying a reagent including an enzyme or the like to the working electrode 5, the counter electrode 6 and the detecting electrode 7, which is exposed from the cutout part 9 of the spacer 8, and a cover 13 which covers the spacer 8, as shown in FIG. 1. The so-constructed biosensor can be formed by a simple method, and a biosensor which is excellent in a measuring accuracy as well as a biosensor in which a reagent layer is placed uniformly on the electrodes regardless of a reagent liquid composition, and which has a uniform performance can be realized.
摘要翻译：根据本发明的生物传感器包括支撑体1，由诸如金，钯等的诸如贵金属的导电材料构成的导电层2和碳，平行于载体侧的狭缝3a和3b 如图1所示，与支撑体1侧垂直的狭缝4a，4b，工作电极5，对电极6，检测电极7，覆盖工作电极5的间隔件8，对置电极6和检测电极7 支撑体1，形成试样供给路径的矩形切口部9，试料供给路径的入口9a，通过将包含酶等的试剂涂布在工作电极5上而形成的试剂层12，对电极6和从间隔件8的切口部9暴露的检测电极7和覆盖间隔件8的盖13，如图1所示。如此构造的生物传感器可以通过简单的方法形成，并且测量精度优异的生物传感器以及试剂层均匀地放置在电极上而不考虑试剂液体组成的生物传感器，并且其中具有统一的性能可以实现。

5. 发明授权

US07998325B2 Biosensor, thin film electrode forming method, quantification apparatus, and quantification method 有权
标题翻译：生物传感器，薄膜电极形成方法，定量装置和定量方法
公开(公告)号：US07998325B2
公开(公告)日：2011-08-16
申请号：US10809217
申请日：2004-03-25
申请人： Shoji Miyazaki , Hiroyuki Tokunaga , Masaki Fujiwara , Eriko Yamanishi , Yoshinobu Tokuno
发明人： Shoji Miyazaki , Hiroyuki Tokunaga , Masaki Fujiwara , Eriko Yamanishi , Yoshinobu Tokuno
IPC分类号： G01N27/327
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.
摘要翻译：公开了一种生物传感器，其包括支撑件; 由诸如金或钯等贵金属的导电材料和碳组成的导电层; 平行于和垂直于支撑体侧面的狭缝; 工作，计数器和检测电极; 覆盖支架上的工作电极，反电极和检测电极的间隔件; 在间隔件中形成矩形切口，形成试样供应路径; 样品供应路径的入口; 通过将含有酶的试剂施加到通过所述间隔件中的切口暴露的所述工作，反向和检测电极而形成的试剂层; 和间隔物上的盖子。可以通过简单的方法形成生物传感器，并且不管试剂组成如何，在电极上提供均匀的试剂层。

6. 发明申请

US20110174613A1 Biosensor, thin film electrode forming method, quantification apparatus, and quantification method 有权
公开(公告)号：US20110174613A1
公开(公告)日：2011-07-21
申请号：US12930627
申请日：2011-01-11
申请人： Shoji Miyazaki , Hiroyuki Tokunaga , Masaki Fujiwara , Eriko Yamanishi , Yoshinobu Tokuno
发明人： Shoji Miyazaki , Hiroyuki Tokunaga , Masaki Fujiwara , Eriko Yamanishi , Yoshinobu Tokuno
IPC分类号： G01N27/403
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.

7. 发明申请

US20100006432A1 Biosensor 审中-公开
标题翻译：生物传感器
公开(公告)号：US20100006432A1
公开(公告)日：2010-01-14
申请号：US12456199
申请日：2009-06-12
申请人： Shoji Miyazaki , Hiroyuki Tokunaga , Masaki Fujiwara , Takahiro Nakaminami , Junko Nakayama , Eriko Yamanishi
发明人： Shoji Miyazaki , Hiroyuki Tokunaga , Masaki Fujiwara , Takahiro Nakaminami , Junko Nakayama , Eriko Yamanishi
IPC分类号： G01N27/30 , G01N27/26
CPC分类号： C12Q1/003
摘要： In a biosensor for measuring a specific substance in a liquid sample, one or a combination of sugar alcohol, metallic salt, organic acid or organic acid salt which has at least one carboxyl group in a molecule, and organic acid or organic acid salt which has at least one carboxyl group and one amino group in a molecule, is included in a reagent layer provided on electrodes, thereby providing a highly-accurate biosensor which is excellent in stability and has high response (sensitivity, linearity) of the sensor to the substrate concentration.
摘要翻译：在用于测量液体样品中的特定物质的生物传感器中，分子中具有至少一个羧基的糖醇，金属盐，有机酸或有机酸盐的一种或组合，以及有机酸或有机酸盐，其具有分子中的至少一个羧基和一个氨基被包含在设置在电极上的试剂层中，从而提供稳定性优异并且具有高传感器对基板的响应（灵敏度，线性）的高精度生物传感器浓度。

8. 发明授权

US06911131B2 Biosensor 有权
标题翻译：生物传感器
公开(公告)号：US06911131B2
公开(公告)日：2005-06-28
申请号：US09979842
申请日：2001-03-28
申请人： Shoji Miyazaki , Hiroyuki Tokunaga , Masaki Fujiwara , Takahiro Nakaminami , Junko Nakayama , Eriko Yamanishi
发明人： Shoji Miyazaki , Hiroyuki Tokunaga , Masaki Fujiwara , Takahiro Nakaminami , Junko Nakayama , Eriko Yamanishi
IPC分类号： C12Q1/00 , G01N27/327 , G01N21/00
CPC分类号： C12Q1/003
摘要： In a biosensor for measuring a specific substance in a liquid sample, one or a combination of sugar alcohol, metallic salt, organic acid or organic acid salt which has at least one carboxyl group in a molecule, and organic acid or organic acid salt which has at least one carboxyl group and one amino group in a molecule, is included in a reagent layer provided on electrodes, thereby providing a highly-accurate biosensor which is excellent in stability and has high response (sensitivity, linearity) of the sensor to the substrate concentration.
摘要翻译：在用于测量液体样品中的特定物质的生物传感器中，分子中具有至少一个羧基的糖醇，金属盐，有机酸或有机酸盐的一种或组合，以及有机酸或有机酸盐，其具有分子中的至少一个羧基和一个氨基被包含在设置在电极上的试剂层中，从而提供稳定性优异并且具有高传感器对基板的响应（灵敏度，线性）的高精度生物传感器浓度。

9. 发明授权

US06875327B1 Biosensor, method of forming thin-film electrode, and method and apparatus for quantitative determination 有权
标题翻译：生物传感器，薄膜电极的形成方法，定量测定方法和装置
公开(公告)号：US06875327B1
公开(公告)日：2005-04-05
申请号：US09889243
申请日：2000-11-14
申请人： Shoji Miyazaki , Hiroyuki Tokunaga , Masaki Fujiwara , Eriko Yamanishi , Yoshinobu Tokuno
发明人： Shoji Miyazaki , Hiroyuki Tokunaga , Masaki Fujiwara , Eriko Yamanishi , Yoshinobu Tokuno
IPC分类号： C12Q1/00 , G01N27/327 , H05H1/00
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： The invention provides a biosensor comprising a support; a conductive layer composed of a electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.
摘要翻译：本发明提供一种生物传感器，其包含支撑体; 由导电材料如贵金属（例如金或钯）和碳组成的导电层; 平行于和垂直于支撑体侧面的狭缝; 工作，计数器和检测电极; 覆盖支架上的工作电极，反电极和检测电极的间隔件; 在间隔件中形成矩形切口，形成试样供应路径; 样品供应路径的入口; 通过将含有酶的试剂施加到通过所述间隔件中的切口暴露的所述工作，反向和检测电极而形成的试剂层; 和间隔物上的盖子。可以通过简单的方法形成生物传感器，并且不管试剂组成如何，在电极上提供均匀的试剂层。

10. 发明申请

US20050036906A1 Biosensor 审中-公开
标题翻译：生物传感器
公开(公告)号：US20050036906A1
公开(公告)日：2005-02-17
申请号：US10751919
申请日：2004-01-07
申请人： Katsuji Nakahara , Kiyohiko Itoh , Shoji Miyazaki , Hiroyuki Tokunaga , Eriko Yamanishi
发明人： Katsuji Nakahara , Kiyohiko Itoh , Shoji Miyazaki , Hiroyuki Tokunaga , Eriko Yamanishi
IPC分类号： G01N33/487 , G01N21/00
CPC分类号： G01N27/3272
摘要： A biosensor for analyzing specific components in an introduced liquid sample by reaction of the liquid sample with a reagent comprises a cavity into which the liquid sample is introduced, an air hole communicating from the cavity to outside, and a water repellent part having a water repellency at 43 mN/m or less in surface free energy and provided to at least a portion around the outlet of the air hole. By the water repellent part around the air hole, the liquid sample can be prevented from flowing out through the air hole, thereby achieving a high-accuracy measurement.
摘要翻译：用于通过液体样品与试剂反应来分析引入的液体样品中的特定成分的生物传感器包括：液体样品被引入的空腔，从腔体到外部连通的空气孔，以及具有防水性的防水部件在表面自由能为43mN / m以下，并且设置在空气孔的出口周围的至少一部分。通过空气孔周围的防水部件，可以防止液体样品通过气孔流出，从而实现高精度测量。

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