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    • 7. 发明申请
    • BIOSENSOR
    • 生物传感器
    • US20090065356A1
    • 2009-03-12
    • US12297676
    • 2007-04-19
    • Junko NakayamaYoshifumi TakaharaEriko YamanishiYoshihiro Itoh
    • Junko NakayamaYoshifumi TakaharaEriko YamanishiYoshihiro Itoh
    • G01N27/26
    • C12Q1/006
    • In a biosensor for measuring glucose in a liquid sample, an additive agent which is one of an organic acid or organic acid salt having at least one carboxyl group in a molecule, an organic acid or organic acid salt having at least one amino group or carbonyl group in a molecule, a sugar alcohol, and a solubilized protein, or a combination thereof is added into a reagent layer including glucose dehydrogenase having flavin adenine dinucleotide as a coenzyme, which is provided on an insulating substrate. Thereby, the substrate specificity to glucose and the preservation stability can be enhanced, and further, reactions to sugars other than glucose can be avoided.
    • 在用于测量液体样品中的葡萄糖的生物传感器中,作为分子中具有至少一个羧基的有机酸或有机酸盐之一的添加剂,具有至少一个氨基的有机酸或有机酸盐或羰基 将分子中的一个基团,糖醇和增溶蛋白质或其组合加入到包含设置在绝缘基板上的作为辅酶的具有黄素腺嘌呤二核苷酸的葡萄糖脱氢酶的试剂层中。 因此,可以提高对葡萄糖的底物特异性和保存稳定性,进而可以避免对葡萄糖以外的糖的反应。
    • 8. 发明申请
    • BIOSENSOR
    • 生物传感器
    • US20090134023A1
    • 2009-05-28
    • US12297672
    • 2007-04-19
    • Junko NakayamaYoshifumi TakaharaEriko YamanishiYoshihiro Itoh
    • Junko NakayamaYoshifumi TakaharaEriko YamanishiYoshihiro Itoh
    • G01N27/26G01N33/487C12M1/00
    • C12Q1/006G01N33/5438
    • In a biosensor of the present invention, BSA is contained in a reagent layer including a reagent which reacts specifically with glucose, and the glucose concentration in whole blood as a sample solution is electrochemically measured. When the sensor response characteristics when whole blood is used as the sample solution are measured, the influence by hematocrit to the sensor response characteristics is reduced according to the additive amount of BSA when the hematocrit value is in the range of 25% and below, while no BSA addition effect is recognized in the sensor response characteristics when the hematocrit value is in the range of 25 to 65%. Thereby, the influences by hematocrit and temperature can be reduced, and thus highly precise measurement can be performed.
    • 在本发明的生物传感器中,在包含与葡萄糖特异性反应的试剂的试剂层中含有BSA,作为样品溶液的全血中的葡萄糖浓度进行电化学测定。 当测量全血用作样品溶液时的传感器响应特性,当血细胞比容值在25%以下的范围内时,血细胞比容对传感器响应特性的影响根据BSA的添加量减少,而 当血细胞比容值在25至65%的范围内时,在传感器响应特性中不识别出BSA附加效应。 由此,可以降低血细胞比容和温度的影响,从而可以进行高精度的测量。