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1. 发明授权

US06573102B2 Lytic reagent composition for determination of nucleated blood cells 有权
标题翻译：用于测定有核血细胞的溶血剂试剂组合物
公开(公告)号：US06573102B2
公开(公告)日：2003-06-03
申请号：US09917530
申请日：2001-07-27
申请人： Yi Li , Jing Li
发明人： Yi Li , Jing Li
IPC分类号： G01N3100
CPC分类号： G01N33/80 , G01N15/12 , G01N15/1459 , G01N33/5002 , G01N33/721 , G01N2015/1006 , G01N2015/1493 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： A lytic reagent composition for measuring nucleated blood cells in a blood sample is described. The lytic reagent composition contains a quaternary ammonium surfactant, an ethoxylated phenol, and an ethoxylated alcohol. When mixed with a blood sample, the lytic reagent composition lyses red blood cells and enables a differentiation of nucleated red blood cells from other cell types by DC impedance measurement. The lytic reagent composition can further contain an organic ligand for determining total hemoglobin concentration of a blood sample photometrically. Further disclosed is a lytic reagent system including the lytic reagent composition and a diluent. In addition, a single reagent composition containing salts is also disclosed, which can be used without a separate diluent. The lytic reagent compositions can be used for concurrent measurement of nucleated red blood cells, WBC, and hemoglobin of a blood sample.
摘要翻译：描述了用于测量血液样品中的有核血细胞的溶解试剂组合物。溶解试剂组合物含有季铵表面活性剂，乙氧基化苯酚和乙氧基化醇。当与血液样品混合时，溶解试剂组合物溶解红细胞，并且通过DC阻抗测量使得有核红细胞与其它细胞类型分化。溶解试剂组合物还可以含有用于测定血样的总血红蛋白浓度的有机配体。还公开了包括溶解试剂组合物和稀释剂的裂解试剂系统。此外，还公开了含有盐的单一试剂组合物，其可在没有单独的稀释剂的情况下使用。裂解试剂组合物可用于同时测量血液样品的有核红细胞，WBC和血红蛋白。

2. 发明授权

US06214625B1 Composition and method for differentiation of basophils and eosinophils in blood 失效
标题翻译：血液中嗜碱性粒细胞和嗜酸性粒细胞分化的组成和方法
公开(公告)号：US06214625B1
公开(公告)日：2001-04-10
申请号：US09301426
申请日：1999-04-28
申请人： Yi Li , Jing Li , Carole Jo Young
发明人： Yi Li , Jing Li , Carole Jo Young
IPC分类号： G01N3100
CPC分类号： G01N33/5094 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/25 , Y10T436/25125
摘要： This invention relates to lytic reagents and methods of using the lytic reagents for automatically determining leukocyte subpopulations in blood. More specifically, the new lytic reagents selectively lyse red blood cells and certain leukocyte subpopulations, which enables the differentiation of at least one subpopulation of leukocytes. The lytic reagents contain an ethoxylated long chain amine, a quaternary ammonium salt and an acid. When used in combination with a second lytic reagent system, one is able to obtain at least a five part differential of leukocytes by impedance and light scatter measurements, by impedance and radio frequency measurements, or by radio frequency and light scatter measurements.
摘要翻译：本发明涉及溶解试剂和使用裂解试剂自动测定血液中白细胞亚群的方法。更具体地，新的溶解试剂选择性地裂解红细胞和某些白细胞亚群，其能够分化白细胞的至少一个亚群。裂解试剂含有乙氧基化长链胺，季铵盐和酸。当与第二溶解试剂系统组合使用时，可以通过阻抗和光散射测量，通过阻抗和射频测量，或通过射频和光散射测量获得白细胞的至少五分之一的差异。

3. 发明授权

US5917584A Method for differentiation of nucleated red blood cells 失效
公开(公告)号：US5917584A
公开(公告)日：1999-06-29
申请号：US10886
申请日：1998-01-22
申请人： Yi Li , Jing Li , Carole Young
发明人： Yi Li , Jing Li , Carole Young
IPC分类号： G01N33/49 , G01N15/14 , G01N33/50 , G01N33/80 , G01N33/48
CPC分类号： G01N15/1456 , G01N33/80
摘要： A method is provided for differentiation of nucleated red blood cells. In addition, the method provides for a concurrent differentiation of leukocytes in a blood cell sample by suitable electronic and optical measurements. The method includes exposing a blood cell sample to a reagent system to lyse mature red blood cells and subsequently analyzing nucleated red blood cells in a flow cell by optical analysis. A concurrent differentiation of nucleated blood cells and leukocytes can be performed using electronic and optical analysis. The electronic and optical analysis includes light scatter and impedance measurements. This method eliminates the use of nuclear stain for identification of nucleated red blood cells. The method of the present invention, for the first time, reports differentiation and enumeration of nucleated red blood cells without using fluorescence.

4. 发明授权

US5874310A Method for differentiation of nucleated red blood cells 失效
标题翻译：有核红细胞分化方法
公开(公告)号：US5874310A
公开(公告)日：1999-02-23
申请号：US975846
申请日：1997-11-21
申请人： Yi Li , Jing Li , Carole Young
发明人： Yi Li , Jing Li , Carole Young
IPC分类号： G01N33/50 , G01N15/14 , G01N33/49 , G01N33/80 , G01N31/00
CPC分类号： G01N33/80 , G01N15/1459 , G01N2015/1006 , G01N2015/1402 , Y10T436/101666 , Y10T436/25125
摘要： A method is provided for differentiation of nucleated red blood cells. In addition, the method provides for a concurrent differentiation of leukocytes in a blood cell sample by suitable electronic and optical measurements. The method includes exposing a blood cell sample to a reagent system to lyse mature red blood cells and subsequently analyzing nucleated red blood cells in a flow cell by optical analysis. A concurrent differentiation of nucleated blood cells and leukocytes can be performed using electronic and optical analysis. The electronic and optical analysis includes light scatter and impedance measurements. This method eliminates the use of nuclear stain for identification of nucleated red blood cells. The method of the present invention, for the first time, reports differentiation and enumeration of nucleated red blood cells without using fluorescence.
摘要翻译：提供了一种用于分化有核红细胞的方法。此外，该方法通过合适的电子和光学测量提供血细胞样品中白细胞的同时分化。该方法包括将血液细胞样品暴露于试剂系统以裂解成熟红细胞，随后通过光学分析分析流动池中的有核红细胞。可以使用电子和光学分析进行有核血细胞和白细胞的并发分化。电子和光学分析包括光散射和阻抗测量。该方法消除了使用核染色鉴定有核红细胞。本发明的方法首次报道了不使用荧光的有核红细胞的分化和计数。

5. 发明授权

US07482165B2 Method of preventing white blood cell interferences to red blood cell measurements of a blood sample 有权
标题翻译：防止白细胞干扰血液样本红细胞测量的方法
公开(公告)号：US07482165B2
公开(公告)日：2009-01-27
申请号：US11210346
申请日：2005-08-24
申请人： Min Zheng , Jing Li , Yi Li , Michael J. Switzer
发明人： Min Zheng , Jing Li , Yi Li , Michael J. Switzer
IPC分类号： G01N33/48
CPC分类号： G01N33/491 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/25 , Y10T436/25125
摘要： Methods of prevention and correction of white blood cell interferences to the measurements of mean cell volume (MCV), red blood cell concentration (RBC) and hematocrit (Hct) of a blood sample are disclosed. The interference to MCV can be prevented by identifying a valley between the red blood cell and white blood cell modes on the red blood cell distribution histogram, defining a red blood cell region using the valley and calculating MCV within the defined region. Alternatively, a curve fit of the white blood cell population on the red blood cell distribution histogram can be used to exclude the white blood cells. RBC can be corrected by subtracting the white blood cell concentration obtained from the analysis of a second aliquot sample, when the white blood cell concentration (WBC) exceeds a predetermined criterion. Hct of the blood sample can be calculated using the obtained MCV and RBC.
摘要翻译：公开了对血液样本的平均细胞体积（MCV），红细胞浓度（RBC）和血细胞比容（Hct）的测量的白细胞干扰的预防和校正方法。通过在红细胞分布直方图上识别红细胞和白细胞模式之间的谷，可以防止对MCV的干扰，使用谷定义红细胞区域并计算定义区域内的MCV。或者，可以使用白细胞群体对红细胞分布直方图的曲线拟合来排除白细胞。当白细胞浓度（WBC）超过预定标准时，可通过减去从第二等份样品的分析获得的白细胞浓度来校正RBC。可以使用获得的MCV和RBC计算血液样品的Hct。

6. 发明授权

US07198953B2 Method of using a reference control composition for measurement of nucleated red blood cells 有权
标题翻译：使用参照对照组合物测定有核红细胞的方法
公开(公告)号：US07198953B2
公开(公告)日：2007-04-03
申请号：US10960370
申请日：2004-10-07
申请人： Nery Ortiz , Jing Li , Yi Li , Sandra Socarras
发明人： Nery Ortiz , Jing Li , Yi Li , Sandra Socarras
IPC分类号： G01N33/48 , G01N31/00
CPC分类号： G01N33/96 , G01N15/1459 , G01N2015/1006 , G01N2015/1402 , G01N2496/05 , Y10T436/101666
摘要： Methods of using a reference control composition containing a nucleated red blood cell component for measurement of nucleated red blood cells are disclosed. The nucleated red blood cell component is made of stabilized or processed nucleated blood cells which have impedance and optical properties simulating the impedance and optical properties of human nucleated red blood cells under a blood lysing condition as measured by a specific measurement. The methods include mixing the reference control composition with a lytic reagent, and analyzing the control sample mixture on a flow cytometric instrument by impedance, impedance and optical measurement, or DC impedance and a multi-dimensional measurement of light scatter, radio frequency and a second DC impedance; and reporting the simulated nucleated red blood cells in the reference control composition.
摘要翻译：公开了使用含有核红细胞成分的参照对照组合物测定有核红细胞的方法。有核红细胞成分由稳定或加工的有核血细胞制成，其具有模拟通过特异性测量测量的血液裂解条件下的人核红细胞的阻抗和光学性质的阻抗和光学性质。所述方法包括将参考对照组合物与裂解试剂混合，并通过阻抗，阻抗和光学测量或DC阻抗和光散射，射频和第二次的多维测量在流式细胞仪上分析对照样品混合物直流阻抗; 并报告参考对照组合物中的模拟有核红细胞。

7. 发明申请

US20050079623A1 Method of using a reference control composition for measurement of nucleated red blood cells 有权
标题翻译：使用参照对照组合物测定有核红细胞的方法
公开(公告)号：US20050079623A1
公开(公告)日：2005-04-14
申请号：US10960370
申请日：2004-10-07
申请人： Nery Ortiz , Jing Li , Yi Li , Sandra Socarras
发明人： Nery Ortiz , Jing Li , Yi Li , Sandra Socarras
IPC分类号： G01N33/48 , G01N33/96
CPC分类号： G01N33/96 , G01N15/1459 , G01N2015/1006 , G01N2015/1402 , G01N2496/05 , Y10T436/101666
摘要： Methods of using a reference control composition containing a nucleated red blood cell component for measurement of nucleated red blood cells are disclosed. The nucleated red blood cell component is made of stabilized or processed nucleated blood cells which have impedance and optical properties simulating the impedance and optical properties of human nucleated red blood cells under a blood lysing condition as measured by a specific measurement. The methods include mixing the reference control composition with a lytic reagent, and analyzing the control sample mixture on a flow cytometric instrument by impedance, impedance and optical measurement, or DC impedance and a multi-dimensional measurement of light scatter, radio frequency and a second DC impedance; and reporting the simulated nucleated red blood cells in the reference control composition.
摘要翻译：公开了使用含有核红细胞成分的参照对照组合物测定有核红细胞的方法。有核红细胞成分由稳定或加工的有核血细胞制成，其具有模拟通过特异性测量测量的血液裂解条件下的人核红细胞的阻抗和光学性质的阻抗和光学性质。所述方法包括将参考对照组合物与裂解试剂混合，并通过阻抗，阻抗和光学测量或DC阻抗和光散射，射频和第二次的多维测量在流式细胞仪上分析对照样品混合物直流阻抗; 并报告参考对照组合物中的模拟有核红细胞。

8. 发明授权

US06410330B1 Method for measurement of nucleated red blood cells 有权
标题翻译：有核红细胞测定方法
公开(公告)号：US06410330B1
公开(公告)日：2002-06-25
申请号：US09917533
申请日：2001-07-27
申请人： Yi Li , Jing Li , Ted W. Britton
发明人： Yi Li , Jing Li , Ted W. Britton
IPC分类号： G01N3100
CPC分类号： G01N15/12 , G01N33/80 , G01N2015/0073 , G01N2015/008 , G01N2015/1062 , Y10T436/10 , Y10T436/101666 , Y10T436/107497
摘要： A method for differentiating and enumerating nucleated red blood cells in a blood sample is described. The method includes the steps of lysing red blood cells of a blood sample with a lytic reagent, measuring nucleated blood cells by DC impedance measurement in a non-focused flow aperture, differentiating nucleated red blood cells from other cell types, and reporting nucleated red blood cells in the blood sample. The method further includes subtracting nucleated red blood cells and other interference materials from the count of remaining blood cells, and reporting a corrected white blood cell count of the blood sample. Additionally, the method further includes measuring spectrophotometric absorbance of the sample mixture at a predetermined wavelength of a hemoglobin chromogen formed upon lysing the blood sample, and reporting hemoglobin concentration of the blood sample.
摘要翻译：描述了一种用于分离和计数血液样品中有核红细胞的方法。该方法包括用溶解试剂裂解血液样品的红细胞的步骤，通过非聚焦流动孔中的直流阻抗测量测量有核血细胞，将有核红细胞与其他细胞类型区分开，并报告有核红血血样中的细胞。该方法还包括从剩余血细胞计数中减去有核红细胞和其他干扰物质，并报告血样的校正白细胞计数。此外，该方法还包括在裂解血液样品时形成的血红蛋白色原体的预定波长测量样品混合物的分光光度吸光度，并报告血液样品的血红蛋白浓度。

9. 发明授权

US5874311A Method for differentiation of reticulocytes in blood 失效
标题翻译：血液中网织红细胞分化方法
公开(公告)号：US5874311A
公开(公告)日：1999-02-23
申请号：US976226
申请日：1997-11-21
申请人： Yi Li , Jing Li , Carole Young
发明人： Yi Li , Jing Li , Carole Young
IPC分类号： G01N33/48 , G01N15/14 , G01N33/49 , G01N33/80 , G01N31/00
CPC分类号： G01N33/80 , G01N15/12 , G01N15/1459 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , Y10T436/101666 , Y10T436/25125
摘要： A method is provided for differentiation of reticulocytes. In addition, the method provides for a concurrent differentiation of leukocyte subpopulations in a blood cell sample by suitable electronic and optical measurements. The method includes exposing a blood cell sample to a reagent system to lyse mature red blood cells and subsequently analyzing reticulocytes in a flow cell by optical analysis. A concurrent differentiation of reticulocytes and leukocytes can be performed using electronic and optical analysis. The electronic and optical analysis includes light scatter and impedance measurements. This method does not require the use of nuclear stain for differentiation of reticulocytes.
摘要翻译：提供了一种分化网织红细胞的方法。此外，该方法通过合适的电子和光学测量提供血细胞样品中白细胞亚群的同时分化。该方法包括将血细胞样品暴露于试剂系统以裂解成熟红细胞，并随后通过光学分析分析流动池中的网织红细胞。网织红细胞和白细胞的并发分化可以使用电子和光学分析进行。电子和光学分析包括光散射和阻抗测量。该方法不需要使用核染色来分化网织红细胞。

10. 发明申请

US20070054403A1 Method of preventing white blood cell interferences to red blood cell measurements of a blood sample 有权
标题翻译：防止白细胞干扰血液样本红细胞测量的方法
公开(公告)号：US20070054403A1
公开(公告)日：2007-03-08
申请号：US11210346
申请日：2005-08-24
申请人： Min Zheng , Jing Li , Yi Li , Michael Switzer
发明人： Min Zheng , Jing Li , Yi Li , Michael Switzer
IPC分类号： G01N33/48
CPC分类号： G01N33/491 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/25 , Y10T436/25125
摘要： Methods of prevention and correction of white blood cell interferences to the measurements of mean cell volume (MCV), red blood cell concentration (RBC) and hematocrit (Hct) of a blood sample are disclosed. The interference to MCV can be prevented by identifying a valley between the red blood cell and white blood cell modes on the red blood cell distribution histogram, defining a red blood cell region using the valley and calculating MCV within the defined region. Alternatively, a curve fit of the white blood cell population on the red blood cell distribution histogram can be used to exclude the white blood cells. RBC can be corrected by subtracting the white blood cell concentration obtained from the analysis of a second aliquot sample, when the white blood cell concentration (WBC) exceeds a predetermined criterion. Hct of the blood sample can be calculated using the obtained MCV and RBC.
摘要翻译：公开了对血液样本的平均细胞体积（MCV），红细胞浓度（RBC）和血细胞比容（Hct）的测量的白细胞干扰的预防和校正方法。通过在红细胞分布直方图上识别红细胞和白细胞模式之间的谷，可以防止对MCV的干扰，使用谷定义红细胞区域并计算定义区域内的MCV。或者，可以使用白细胞群体对红细胞分布直方图的曲线拟合来排除白细胞。当白细胞浓度（WBC）超过预定标准时，可通过减去从第二等份样品的分析获得的白细胞浓度来校正RBC。可以使用获得的MCV和RBC计算血液样品的Hct。

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