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1. 发明申请

US20120123722A1 FLUORESCENT SPECTRUM CORRECTING METHOD AND FLUORESCENT SPECTRUM MEASURING DEVICE 审中-公开
标题翻译：荧光光谱校正方法和荧光光谱测量装置
公开(公告)号：US20120123722A1
公开(公告)日：2012-05-17
申请号：US13287459
申请日：2011-11-02
申请人： Masaya Kakuta , Koji Futamura , Yoshitsugu Sakai , Yasunobu Kato
发明人： Masaya Kakuta , Koji Futamura , Yoshitsugu Sakai , Yasunobu Kato
IPC分类号： G06F19/00
CPC分类号： G01N21/274 , G01N15/1429 , G01N21/6428 , G01N2015/0065
摘要： A fluorescent spectrum correcting method includes comparing fluorescent spectrum obtained from micro-particles labeled with a plurality of fluorescent pigments with reference spectrum to separating the fluorescent spectrum into fluorescent spectrum for each pigment, and previously measured spectrum data is used as the reference spectrum.
摘要翻译：荧光光谱校正方法包括将由多个荧光颜料标记的微粒子获得的荧光光谱与参考光谱进行比较，将荧光光谱分离成每个颜料的荧光光谱，并将先前测量的光谱数据用作参考光谱。

2. 发明授权

US08990024B2 Fluorescence intensity compensation method and fluorescence intensity calculation device 有权
标题翻译：荧光强度补偿方法和荧光强度计算装置
公开(公告)号：US08990024B2
公开(公告)日：2015-03-24
申请号：US13178172
申请日：2011-07-07
申请人： Yasunobu Kato , Yoshitsugu Sakai
发明人： Yasunobu Kato , Yoshitsugu Sakai
IPC分类号： G06F19/16 , G06F19/10 , G01N15/14 , G01N21/53 , G01N21/64 , G01N21/47
CPC分类号： G01N15/1429 , G01N15/1459 , G01N21/53 , G01N21/6428 , G01N21/645 , G01N2021/4707 , G01N2021/4726 , G01N2021/6421 , G01N2021/6439 , G01N2201/1293
摘要： A fluorescence intensity compensation method, includes: receiving, with photodetectors having different input wavelength bands, fluorescence emitted from fluorochromes excited by irradiating light on microparticles multiply-labeled by a plurality of fluorochromes with overlapping fluorescence wavelengths; collecting detected values for the photodetectors; and obtaining a measurement spectrum, by approximating, from the linear sum of single-stain spectrums obtained from microparticles individually labeled with the fluorochromes; wherein approximation of the measurement spectrum by the linear sum of the single-stain spectrums is performed using the restricted least-square method.
摘要翻译：一种荧光强度补偿方法，包括：利用具有不同输入波长带的光电检测器，通过用多个具有重叠荧光波长的荧光色素多标记的微粒上照射光而激发的荧光发射的荧光; 收集光检测器的检测值; 通过从由荧光色素单独标记的微粒获得的单色光谱的线性和近似得到测量光谱; 其中使用限制最小二乘法来执行单色光谱的线性和的测量光谱的近似。

3. 发明申请

US20120010822A1 FLUORESCENCE INTENSITY COMPENSATION METHOD AND FLUORESCENCE INTENSITY CALCULATION DEVICE 有权
标题翻译：荧光强度补偿方法和荧光强度计算装置
公开(公告)号：US20120010822A1
公开(公告)日：2012-01-12
申请号：US13178172
申请日：2011-07-07
申请人： Yasunobu Kato , Yoshitsugu Sakai
发明人： Yasunobu Kato , Yoshitsugu Sakai
IPC分类号： G06F19/16
CPC分类号： G01N15/1429 , G01N15/1459 , G01N21/53 , G01N21/6428 , G01N21/645 , G01N2021/4707 , G01N2021/4726 , G01N2021/6421 , G01N2021/6439 , G01N2201/1293
摘要： A fluorescence intensity compensation method, includes: receiving, with photodetectors having different input wavelength bands, fluorescence emitted from fluorochromes excited by irradiating light on microparticles multiply-labeled by a plurality of fluorochromes with overlapping fluorescence wavelengths; collecting detected values for the photodetectors; and obtaining a measurement spectrum, by approximating, from the linear sum of single-stain spectrums obtained from microparticles individually labeled with the fluorochromes; wherein approximation of the measurement spectrum by the linear sum of the single-stain spectrums is performed using the restricted least-square method.
摘要翻译：一种荧光强度补偿方法，包括：利用具有不同输入波长带的光电检测器，通过用多个具有重叠荧光波长的荧光色素多标记的微粒上照射光而激发的荧光发射的荧光; 收集光检测器的检测值; 通过从由荧光色素单独标记的微粒获得的单色光谱的线性和近似得到测量光谱; 其中使用限制最小二乘法来执行单色光谱的线性和的测量光谱的近似。

4. 发明授权

US08825431B2 Fluorescence intensity correcting method, fluorescence intensity calculating method, and fluorescence intensity calculating apparatus 有权
标题翻译：荧光强度校正方法，荧光强度计算方法和荧光强度计算装置
公开(公告)号：US08825431B2
公开(公告)日：2014-09-02
申请号：US13089961
申请日：2011-04-19
申请人： Yasunobu Kato , Yoshitsugu Sakai
发明人： Yasunobu Kato , Yoshitsugu Sakai
IPC分类号： G06F3/01 , G06F3/03 , G06F3/12 , G06F13/10
CPC分类号： G01N21/6428 , G01N2021/6421 , G01N2021/6439
摘要： A fluorescence intensity calculating apparatus, includes: a measuring section configured to receive fluorescences generated from plural fluorescent dyes excited by radiating a light to a microparticle multiply-labeled with the plural fluorescent dyes having fluorescence wavelength bands overlapping one another by photodetectors which correspond to different received light wavelength bands, respectively, and whose number is larger than the number of fluorescent dyes, and obtain measured spectra by collecting detected values from the photodetectors; and a calculating section configured to approximate the measured spectra based on a linear sum of single-dyeing spectra obtained from the microparticle individually labeled with the fluorescent dyes, thereby calculating intensities of the fluorescences generated from the fluorescent dyes, respectively.
摘要翻译：一种荧光强度计算装置，包括：测量部分，其被配置为接收由多个荧光染料产生的荧光，所述荧光由通过辐射光而被激发的微粒被多个荧光染料多个标记，所述荧光染料具有相互重叠的荧光波长带，光波长带，其数量大于荧光染料的数量，并通过从光电检测器收集检测值获得测量的光谱; 以及计算部，其基于从由荧光染料分别标记的微粒获得的单染色谱的线性和，近似测定的光谱，由此分别计算由荧光染料产生的荧光的强度。

5. 发明申请

US20120016616A1 FLUORESCENCE INTENSITY CORRECTING METHOD, FLUORESCENCE INTENSITY CALCULATING METHOD, AND FLUORESCENCE INTENSITY CALCULATING APPARATUS 有权
标题翻译：荧光强度校正方法，荧光强度计算方法和荧光强度计算装置
公开(公告)号：US20120016616A1
公开(公告)日：2012-01-19
申请号：US13089961
申请日：2011-04-19
申请人： Yasunobu Kato , Yoshitsugu Sakai
发明人： Yasunobu Kato , Yoshitsugu Sakai
IPC分类号： G01D18/00 , G06F19/00
CPC分类号： G01N21/6428 , G01N2021/6421 , G01N2021/6439
摘要： A fluorescence intensity calculating apparatus, includes: a measuring section configured to receive fluorescences generated from plural fluorescent dyes excited by radiating a light to a microparticle multiply-labeled with the plural fluorescent dyes having fluorescence wavelength bands overlapping one another by photodetectors which correspond to different received light wavelength bands, respectively, and whose number is larger than the number of fluorescent dyes, and obtain measured spectra by collecting detected values from the photodetectors; and a calculating section configured to approximate the measured spectra based on a linear sum of single-dyeing spectra obtained from the microparticle individually labeled with the fluorescent dyes, thereby calculating intensities of the fluorescences generated from the fluorescent dyes, respectively.
摘要翻译：一种荧光强度计算装置，包括：测量部分，其被配置为接收由多个荧光染料产生的荧光，所述荧光由通过辐射光而被激发的微粒被多个荧光染料多个标记，所述荧光染料具有相互重叠的荧光波长带，光波长带，其数量大于荧光染料的数量，并通过从光电检测器收集检测值获得测量的光谱; 以及计算部，其基于从由荧光染料分别标记的微粒获得的单染色谱的线性和，近似测定的光谱，由此分别计算由荧光染料产生的荧光的强度。

6. 发明申请

US20120056103A1 FLUORESCENCE INTENSITY COMPENSATION METHOD AND FLUORESCENCE INTENSITY COMPUTING APPARATUS 有权
标题翻译：荧光强度补偿方法和荧光强度计算装置
公开(公告)号：US20120056103A1
公开(公告)日：2012-03-08
申请号：US13212324
申请日：2011-08-18
申请人： Yoshitsugu Sakai
发明人： Yoshitsugu Sakai
IPC分类号： G01N21/64
CPC分类号： G01N21/64 , G01N15/1429 , G01N2021/6421 , G01N2021/6441
摘要： A fluorescence intensity compensation method includes emitting light onto microparticles multiply labeled with a plurality of fluorochromes having fluorescence wavelength ranges that overlap each other in order to excite the fluorochromes and receiving fluorescence generated by the excited fluorochromes using photodetectors having different reception wavelength ranges, and computing fluorescence intensities of the fluorochromes by compensating detection values of the photodetectors under a predetermined constraint condition imposed on the computed fluorescence intensities.
摘要翻译：荧光强度补偿方法包括：使用多个具有彼此重叠的荧光波长范围的荧光染料标记的微粒发光，以便使用具有不同接收波长范围的光检测器激发荧光染料并接收由激发的荧光染料产生的荧光，并计算荧光通过在施加在计算出的荧光强度的预定约束条件下补偿光电探测器的检测值来补偿荧光染料的强度。

7. 发明授权

US07110153B2 Optical apparatus and laser display apparatus having laser beam scanner each 失效
公开(公告)号：US07110153B2
公开(公告)日：2006-09-19
申请号：US11113293
申请日：2005-04-25
申请人： Yoshitsugu Sakai
发明人： Yoshitsugu Sakai
IPC分类号： G02B26/08
CPC分类号： G02B26/0808
摘要： An optical apparatus is disclosed which has a scanner for scanning a predetermined area with a laser beam. The optical apparatus comprises: a laser source; a scanner having a scanning mirror for causing a laser beam generated by the laser source to scan; an angle sensor for sensing angles of the scanning mirror of the scanner; a fault detection block for determining whether the scanning mirror operates safely and normally based at least on angle information coming from the angle sensor; and a projection control block for controlling projection of the laser beam based on fault detection information coming from the fault detection block.

8. 发明申请

US20050190422A1 Optical apparatus and laser display apparatus having laser beam scanner each 失效
公开(公告)号：US20050190422A1
公开(公告)日：2005-09-01
申请号：US11113280
申请日：2005-04-25
申请人： Yoshitsugu Sakai
发明人： Yoshitsugu Sakai
IPC分类号： G02B26/10 , G02B26/08 , H04N3/08 , G06K7/10
CPC分类号： G02B26/0808
摘要： An optical apparatus is disclosed which has a scanner for scanning a predetermined area with a laser beam. The optical apparatus comprises: a laser source; a scanner having a scanning mirror for causing a laser beam generated by the laser source to scan; an angle sensor for sensing angles of the scanning mirror of the scanner; a fault detection block for determining whether the scanning mirror operates safely and normally based at least on angle information coming from the angle sensor; and a projection control block for controlling projection of the laser beam based on fault detection information coming from the fault detection block.

9. 发明授权

US09619907B2 Microparticle analyzing apparatus and data displaying method 有权
公开(公告)号：US09619907B2
公开(公告)日：2017-04-11
申请号：US13089569
申请日：2011-04-19
申请人： Yoshitsugu Sakai
发明人： Yoshitsugu Sakai
IPC分类号： G06T11/20 , G01J1/58 , G01N21/64 , G01N15/14
CPC分类号： G06T11/206 , G01N15/1463 , G01N21/6428 , G01N21/645 , G01N2021/6421 , G01N2021/6423 , G01N2021/6441 , G06T11/001
摘要： Disclosed herein is a microparticle analyzing apparatus including a detecting portion configured to simultaneously detect a fluorescence generated from a microparticle in plural wavelength regions and a displaying portion configured to display thereon detection results in the plural wavelength regions in a form of a spectrum.

10. 发明申请

US20120049086A1 FLUORESCENCE INTENSITY CORRECTION METHOD, FLUORESCENCE INTENSITY CALCULATION METHOD, AND FLUORESCENCE INTENSITY CALCULATION DEVICE AND FLUORESCENCE INTENSITY CORRECTION PROGRAM 有权
标题翻译：荧光强度校正方法，荧光强度计算方法和荧光强度计算装置和荧光强度校正程序
公开(公告)号：US20120049086A1
公开(公告)日：2012-03-01
申请号：US13196102
申请日：2011-08-02
申请人： Yoshitsugu Sakai
发明人： Yoshitsugu Sakai
IPC分类号： G01N21/64
CPC分类号： G01N21/6428 , G01N21/274 , G01N2021/6417
摘要： A fluorescence intensity correction method including: receiving fluorescence emitted from fluorescent pigments, collecting detected values from each light detector, and obtaining a fluorescence spectrum of each fluorescent pigment as one spectrum group; separating the obtained spectrum group into a plurality of small spectrum groups; comparing the separated small spectrum groups with a fluorescence wavelength distribution of each fluorescent pigment obtained in advance and specifying the small spectrum groups as the fluorescence spectrum of any fluorescent pigment; comparing a differential spectrum between small spectrum groups that are not specified and one or more specified small spectrum groups with the fluorescence wavelength distribution of unspecified fluorescent pigments obtained in advance, and specifying the differential spectrum as the fluorescence spectrum of any fluorescent pigment; and calculating the intensity of fluorescence emitted from each fluorescent pigment.
摘要翻译：一种荧光强度校正方法，包括：从荧光颜料发射的荧光，从每个光检测器收集检测值，并获得每个荧光颜料作为一个光谱组的荧光光谱; 将获得的光谱组分离成多个小光谱组; 将分离的小光谱组与预先获得的每种荧光颜料的荧光波长分布进行比较，并指定小光谱组作为任何荧光颜料的荧光光谱; 比较未指定的小光谱组与一个或多个指定的小光谱组与预先获得的未指定的荧光颜料的荧光波长分布之间的差分光谱，并将差示光谱指定为任何荧光颜料的荧光光谱; 并计算从每种荧光颜料发出的荧光强度。

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