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3. 发明申请

US20110300570A1 METHOD AND SYSTEM FOR GENERATING SPATIALLY AND TEMPORALLY CONTROLLABLE CONCENTRATION GRADIENTS 审中-公开
标题翻译：用于产生空间和时间可控浓度梯度的方法和系统
公开(公告)号：US20110300570A1
公开(公告)日：2011-12-08
申请号：US13129190
申请日：2009-11-16
申请人： Ali Khademhosseini , Yanan Du , Matthew Hancock
发明人： Ali Khademhosseini , Yanan Du , Matthew Hancock
IPC分类号： G01N1/00 , C12Q1/02 , B01L3/00 , C12M1/34
CPC分类号： G01N30/34 , B01L3/50273 , B01L3/502776 , B01L2300/0816 , G01N1/405 , G01N2001/4088 , G01N2030/342 , Y10T436/2575 , G01N30/6095
摘要： The ability to rapidly generate concentration gradients of diffusible molecules has important applications in many chemical and biological studies. The present invention is directed to methods and systems for generating spatially and temporally controllable concentration gradients of molecules (i.e. proteins or toxins) in a portable microfluidic device. The formation of the concentration gradients can be initiated by an induced forward flow and further optimized during an induced backward flow. The forward and backward flows can be either passively induced and/or actively pumped. The centimeter-length gradients along the microfluidic channel can be spatially and temporally controlled by the backward flow. The gradient profile was stabilized by stopping the flow. In one example, a stabilized concentration gradient of a cardiac toxin, Alpha-cypermethrin, generated according to the invention was used to test the response of HL-1 cardiac cells in the microfluidic device, which correlated with toxicity data obtained from multi-well plates. The invention can be useful for bio-logical and chemical processes that require rapid generation of concentration gradients in a portable microfluidic device.
摘要翻译：快速产生扩散分子的浓度梯度的能力在许多化学和生物学研究中具有重要的应用。本发明涉及用于在便携式微流体装置中产生分子（即蛋白质或毒素）的空间和时间可控浓度梯度的方法和系统。浓度梯度的形成可以通过诱导的正向流引发并且在诱导的逆流期间进一步优化。前向和后向流可以被动地被诱导和/或被主动泵送。沿着微流体通道的厘米长度梯度可以由反向流动在空间和时间上控制。通过停止流动来稳定梯度分布。在一个实例中，使用根据本发明产生的心脏毒素α-氯氰菊酯的稳定化浓度梯度来测试微流体装置中HL-1心脏细胞的反应，其与从多孔板获得的毒性数据相关。本发明可用于需要在便携式微流体装置中快速产生浓度梯度的生物逻辑和化学过程。

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