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1. 发明申请

US20120058530A1 ENGINEERING THE PATHWAY FOR SUCCINATE PRODUCTION 审中-公开
标题翻译：工程生产的途径
公开(公告)号：US20120058530A1
公开(公告)日：2012-03-08
申请号：US13256460
申请日：2010-04-02
申请人： Xueli Zhang , Kaemwich Jantama , Jonathan C. Moore , Laura R. Jarboe , Keelnatham T. Shanmugam , Lonnie O'Neal Ingram
发明人： Xueli Zhang , Kaemwich Jantama , Jonathan C. Moore , Laura R. Jarboe , Keelnatham T. Shanmugam , Lonnie O'Neal Ingram
IPC分类号： C12P7/46 , C12N1/21
CPC分类号： C12P7/46 , C12N9/88 , C12N9/93 , C12Y401/01 , C12Y604/01001
摘要： This invention relates to the biocatalysts for the efficient production of succinic acid and/or other products from renewable biological feedstocks. The biocatalysts have a very high efficiency for the growth-coupled production of succinic acid and/or other products from carbohydrate feed stocks as a result of both genetic manipulations and metabolic evolution. More specifically, certain biocatalysts of the present invention produce succinic acid at high titers and yield in mineral salts media during simple pH-controlled, batch fermentation without the addition of any exogenous genetic material. The genetic manipulations of the present invention are concerned with the energy-conserving strategies coupled with the elimination of alternative routes for NADH oxidation other than the routes for succinic acid production. The biocatalysts contain glucose-repressed gluconeogenic phosphoenol pyruvate carboxykinase (pck) depressed by genetic modifications and a genetically-inactivated phosphotransferase system. In terms of succinic acid production efficiency, the biocatalysts of the present invention are functionally equivalent to succinate producing rumen bacteria such as Actinobacillus succinogens and Mannheimia succiniproducens with one difference that the biocatalysts are able to achieve this high level of succinic acid production in a minimal salt medium with carbohydrate source as opposed to the requirement for a rich media for succinic acid production by rumen bacteria.
摘要翻译：本发明涉及用于从可再生生物原料有效生产琥珀酸和/或其它产品的生物催化剂。由于遗传操作和代谢进化，生物催化剂对于来自碳水化合物原料的琥珀酸和/或其它产物的生长偶联生产具有非常高的效率。更具体地，本发明的某些生物催化剂在简单的pH控制的间歇式发酵过程中以高滴度产生琥珀酸，并在矿物盐介质中产生，而不添加任何外源遗传物质。本发明的遗传操作涉及除琥珀酸生产途径之外的NADH氧化替代途径的节能策略。生物催化剂含有通过遗传修饰和基因失活的磷酸转移酶系统抑制的葡萄糖抑制的糖原磷酸烯醇丙酮酸羧激酶（pck）。在琥珀酸生产效率方面，本发明的生物催化剂在功能上等同于琥珀酸盐生产的瘤胃细菌如琥珀酸放线杆菌素和人造芽孢杆菌，其生物催化剂能够在最小的盐中达到高水平的琥珀酸生产具有碳水化合物来源的培养基，而不是富含培养基对瘤胃细菌产生琥珀酸的要求。

2. 发明授权

US08691539B2 Materials and methods for efficient succinate and malate production 有权
标题翻译：有效的琥珀酸和苹果酸生产的材料和方法
公开(公告)号：US08691539B2
公开(公告)日：2014-04-08
申请号：US12529826
申请日：2008-03-19
申请人： Kaemwich Jantama , Mark John Haupt , Xueli Zhang , Jonathan C. Moore , Keelnatham T. Shanmugam , Lonnie O'Neal Ingram
发明人： Kaemwich Jantama , Mark John Haupt , Xueli Zhang , Jonathan C. Moore , Keelnatham T. Shanmugam , Lonnie O'Neal Ingram
IPC分类号： C12P7/46 , C12N9/00 , C12N1/22 , C12N15/00 , C07H21/04
CPC分类号： C12P7/46 , C12N15/52
摘要： Genetically engineered microorganisms have been constructed to produce succinate and malate in mineral salt media in pH-controlled batch fermentations without the addition of plasmids or foreign genes. The subject invention also provides methods of producing succinate and malate comprising the culture of genetically modified microorganisms.
摘要翻译：已经构建了基因工程微生物，以在pH调节的分批发酵中的矿物盐介质中产生琥珀酸盐和苹果酸盐，而不添加质粒或外来基因。本发明还提供了生产包含转基因微生物培养物的琥珀酸盐和苹果酸盐的方法。

3. 发明申请

US20100184171A1 Materials and Methods for Efficient Succinate and Malate Production 有权
标题翻译：有效的琥珀酸和苹果酸生产的材料和方法
公开(公告)号：US20100184171A1
公开(公告)日：2010-07-22
申请号：US12529826
申请日：2008-03-19
申请人： Kaemwich Jantama , Mark John Haupt , Xueli Zhang , Jonathan C. Moore , Keelnatham T. Shanmugam , Lonnie O'Neal Ingram
发明人： Kaemwich Jantama , Mark John Haupt , Xueli Zhang , Jonathan C. Moore , Keelnatham T. Shanmugam , Lonnie O'Neal Ingram
IPC分类号： C12P7/46 , C12N1/20 , C12N1/21
CPC分类号： C12P7/46 , C12N15/52
摘要： Genetically engineered microorganisms have been constructed to produce succinate and malate in mineral salt media in pH-controlled batch fermentations without the addition of plasmids or foreign genes. The subject invention also provides methods of producing succinate and malate comprising the culture of genetically modified microorganisms.
摘要翻译：已经构建了基因工程微生物，以在pH调节的分批发酵中的矿物盐介质中产生琥珀酸盐和苹果酸盐，而不添加质粒或外来基因。本发明还提供了生产包含转基因微生物培养物的琥珀酸盐和苹果酸盐的方法。

4. 发明授权

US08426191B2 Materials and methods for efficient lactic acid production 有权
标题翻译：高效乳酸生产的材料和方法
公开(公告)号：US08426191B2
公开(公告)日：2013-04-23
申请号：US12632041
申请日：2009-12-07
申请人： Shengde Zhou , Lonnie O'Neal Ingram , Keelnatham T. Shanmugam , Lorraine Yomano , Tammy B. Grabar , Jonathan C. Moore
发明人： Shengde Zhou , Lonnie O'Neal Ingram , Keelnatham T. Shanmugam , Lorraine Yomano , Tammy B. Grabar , Jonathan C. Moore
IPC分类号： C12N1/21 , C12P7/56
CPC分类号： C12N1/20 , C12P7/56
摘要： The present invention provides derivatives of Escherichia coli constructed for the production of lactic acid. The transformed E. coli of the invention are prepared by deleting the genes that encode competing pathways followed by a growth-based selection for mutants with improved performance. These transformed E. coli are useful for providing an increased supply of lactic acid for use in food and industrial applications.
摘要翻译：本发明提供了用于生产乳酸的大肠杆菌的衍生物。本发明的转化的大肠杆菌通过删除编码竞争性途径的基因，随后是具有改进性能的突变体的基于生长的选择来制备。这些转化的大肠杆菌可用于提供用于食品和工业应用的增加的乳酸供应。

5. 发明授权

US09187772B2 L-malate production by metabolically engineered escherichia coli 有权
标题翻译：通过代谢工程化的大肠杆菌生产L-苹果酸
公开(公告)号：US09187772B2
公开(公告)日：2015-11-17
申请号：US13819773
申请日：2011-09-01
申请人： Xueli Zhang , Xuan Wang , Keelnatham T. Shanmugam , Lonnie O'Neal Ingram
发明人： Xueli Zhang , Xuan Wang , Keelnatham T. Shanmugam , Lonnie O'Neal Ingram
IPC分类号： C12P7/46 , C12N1/20 , C12N9/02 , C12N9/88 , C12P7/42
CPC分类号： C12P7/46 , C12N1/20 , C12N9/001 , C12N9/88 , C12P7/42 , C12Y103/01006 , C12Y401/01031
摘要： A process for the production of malic acid in commercially significant quantities from the carbon compounds by genetically modified bacterial strains (GMBS; also referred to as biocatalysts or genetically modified microorganisms) is disclosed. Microorganisms suitable for the production of malic acid can be cultured in one or two-step processes as disclosed herein.
摘要翻译：公开了通过遗传修饰的细菌菌株（GMBS，也被称为生物催化剂或遗传修饰的微生物）从碳化合物生产苹果酸的商业显着量的方法。适用于生产苹果酸的微生物可以如本文所公开的一步或两步方法培养。

6. 发明授权

US09157102B2 Over-expression of NADH-dependent oxidoreductase (fucO) for increasing furfural or 5-hydroxymethylfurfural tolerance 有权
标题翻译： NADH依赖性氧化还原酶（fucO）的过表达用于增加糠醛或5-羟甲基糠醛耐受性
公开(公告)号：US09157102B2
公开(公告)日：2015-10-13
申请号：US14008015
申请日：2012-03-29
申请人： Elliot N. Miller , Xueli Zhang , Lorraine P. Yomano , Xuan Wang , Keelnatham T. Shanmugam , Lonnie O'Neal Ingram
发明人： Elliot N. Miller , Xueli Zhang , Lorraine P. Yomano , Xuan Wang , Keelnatham T. Shanmugam , Lonnie O'Neal Ingram
IPC分类号： C12P7/56 , C12N9/04 , C12P7/06 , C12P7/10 , C12P7/46
CPC分类号： C12P7/56 , C12N9/0006 , C12P7/06 , C12P7/065 , C12P7/10 , C12P7/46 , C12Y101/01202 , Y02E50/16 , Y02E50/17
摘要： The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.
摘要翻译：本发明涉及NADH依赖性丙二醇氧化还原酶（FucO）可以减少糠醛的发现。这允许用于改善用于产生所需产物的细菌和/或酵母细胞中的糠醛耐受性的新方法。因此，提供了对糠醛和5-羟甲基糠醛（5-HMF）具有增加的耐受性的新型生物催化剂（细菌，真菌或酵母细胞），其是制备和使用这些生物催化剂以制备所需产物的方法。

7. 发明授权

US08124259B2 Enhanced electrical contact to microbes in microbial fuel cells 有权
标题翻译：增强与微生物燃料电池微生物的电接触
公开(公告)号：US08124259B2
公开(公告)日：2012-02-28
申请号：US11577395
申请日：2005-10-20
申请人： Andrew G. Rinzler , Lonnie O'Neal Ingram , Keelnatham T. Shanmugam , Jonathan C. Moore , Zhuangchun Wu
发明人： Andrew G. Rinzler , Lonnie O'Neal Ingram , Keelnatham T. Shanmugam , Jonathan C. Moore , Zhuangchun Wu
IPC分类号： H01M8/16 , C12P3/00
CPC分类号： H01M8/0234 , H01M8/16 , Y02E60/527 , Y10S977/746
摘要： A microbial fuel cell (100) includes an anode compartment (110) including an anode (115) and anolyte (120). The anolyte (120) comprises a plurality of in-vivo cells (125) mixed with a plurality of electrically conducting nano or micro-scale fibers (128), wherein at least a portion of the plurality of electrically conducting fibers (128) are in electrical contact with a surface of the anode (115). A cathode compartment (140) includes a cathode (145) and a catholyte (150). A cation-exchange membrane (155) is disposed between the anode compartment (110) and the cathode compartment (140).
摘要翻译：微生物燃料电池（100）包括阳极室（110），其包括阳极（115）和阳极电解液（120）。阳极电解液（120）包括与多个导电纳米或微尺度纤维（128）混合的多个体内细胞（125），其中多个导电纤维（128）的至少一部分在与阳极（115）的表面电接触。阴极室（140）包括阴极（145）和阴极电解液（150）。阳极交换膜（155）设置在阳极室（110）和阴极室（140）之间。

8. 发明申请

US20140024086A1 OVER-EXPRESSION OF NADH-DEPENDENT OXIDOREDUCTASE (FUCO) FOR INCREASING FURFURAL OR 5-HYDROXYMETHYLFURFURAL TOLERANCE 有权
标题翻译： NADH依赖性氧化还原酶（FUCO）的超表达用于增加糠醛或5-羟基甲基葡萄糖耐量
公开(公告)号：US20140024086A1
公开(公告)日：2014-01-23
申请号：US14008015
申请日：2012-03-29
申请人： Elliot N. Miller , Xueli Zhang , Lorraine P. Yomano , Xuan Wang , Keelnatham T. Shanmugam , Lonnie O'Neal Ingram
发明人： Elliot N. Miller , Xueli Zhang , Lorraine P. Yomano , Xuan Wang , Keelnatham T. Shanmugam , Lonnie O'Neal Ingram
IPC分类号： C12P7/56 , C12P7/06 , C12P7/46
CPC分类号： C12P7/56 , C12N9/0006 , C12P7/06 , C12P7/065 , C12P7/10 , C12P7/46 , C12Y101/01202 , Y02E50/16 , Y02E50/17
摘要： The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.
摘要翻译：本发明涉及NADH依赖性丙二醇氧化还原酶（FucO）可以减少糠醛的发现。这允许用于改善用于产生所需产物的细菌和/或酵母细胞中的糠醛耐受性的新方法。因此，提供了对糠醛和5-羟甲基糠醛（5-HMF）具有增加的耐受性的新型生物催化剂（细菌，真菌或酵母细胞），其是制备和使用这些生物催化剂以制备所需产物的方法。

9. 发明申请

US20130157330A1 L-MALATE PRODUCTION BY METABOLICALLY ENGINEERED ESCHERICHIA COLI 有权
标题翻译：通过代谢工程的ESCHERICHIA COLI生产L-马来酸
公开(公告)号：US20130157330A1
公开(公告)日：2013-06-20
申请号：US13819773
申请日：2011-09-01
申请人： Xueli Zhang , Xuan Wang , Keelnatham T. Shanmugam , Lonnie O'Neal Ingram
发明人： Xueli Zhang , Xuan Wang , Keelnatham T. Shanmugam , Lonnie O'Neal Ingram
IPC分类号： C12P7/46
CPC分类号： C12P7/46 , C12N1/20 , C12N9/001 , C12N9/88 , C12P7/42 , C12Y103/01006 , C12Y401/01031
摘要： A process for the production of malic acid in commercially significant quantities from the carbon compounds by genetically modified bacterial strains (GMBS; also referred to as biocatalysts or genetically modified microorganisms) is disclosed. Microorganisms suitable for the production of malic acid can be cultured in one or two-step processes as disclosed herein.
摘要翻译：公开了通过遗传修饰的细菌菌株（GMBS，也被称为生物催化剂或遗传修饰的微生物）从碳化合物生产苹果酸的商业显着量的方法。适用于生产苹果酸的微生物可以如本文所公开的一步或两步方法培养。

10. 发明授权

US07629162B2 Materials and methods for efficient lactic acid production 有权
标题翻译：高效乳酸生产的材料和方法
公开(公告)号：US07629162B2
公开(公告)日：2009-12-08
申请号：US11501137
申请日：2006-08-08
申请人： Shengde Zhou , Lonnie O'Neal Ingram , Keelnatham T. Shanmugam , Lorraine Yomano , Tammy B. Grabar , Jonathan C. Moore
发明人： Shengde Zhou , Lonnie O'Neal Ingram , Keelnatham T. Shanmugam , Lorraine Yomano , Tammy B. Grabar , Jonathan C. Moore
IPC分类号： C12N1/21 , C12P7/56
CPC分类号： C12N1/20 , C12P7/56
摘要： The present invention provides derivatives of ethanologenic Escherichia coli K011 constructed for the production of lactic acid. The transformed E. coli of the invention are prepared by deleting the genes that encode competing pathways followed by a growth-based selection for mutants with improved performance. These transformed E. coli are useful for providing an increased supply of lactic acid for use in food and industrial applications.
摘要翻译：本发明提供了用于生产乳酸的酿酒酵母大肠杆菌K011的衍生物。本发明的转化的大肠杆菌通过删除编码竞争性途径的基因，随后是具有改进性能的突变体的基于生长的选择来制备。这些转化的大肠杆菌可用于提供用于食品和工业应用的增加的乳酸供应。

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