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1. 发明授权

US07309583B2 Homologous 28-kilodalton immunodominant protein genes of Ehrlichia canis and uses thereof 失效
标题翻译： Ehrlichia canis的同源28千道尔顿免疫显性蛋白基因及其用途
公开(公告)号：US07309583B2
公开(公告)日：2007-12-18
申请号：US10062920
申请日：2002-01-31
申请人： David H. Walker , Xue-Jie Yu , Jere W. McBride
发明人： David H. Walker , Xue-Jie Yu , Jere W. McBride
IPC分类号： C12P21/06 , C07K14/00
CPC分类号： C07K14/29 , A61K39/00 , A61K2039/52 , Y02A50/403
摘要： The present invention is directed to the cloning, sequencing and expression of homologous immunoreactive 28-kDa protein genes, p28-1, -2, -3, -5, -6, -7, -9, from a polymorphic multiple gene family of Ehrlichia canis. Further disclosed is a multigene locus encoding all nine homologous 28-kDa protein genes of Ehrlichia canis. Recombinant Ehrlichia canis 28-kDa proteins react with convalescent phase antiserum from an E. canis-infected dog, and may be useful in the development of vaccines and serodiagnostics that are particularly effective for disease prevention and serodiagnosis.
摘要翻译：本发明涉及来自多态性多基因家族的同源免疫反应性28-kDa蛋白基因p28-1，-2，-3，-5，-6，-7，-9的克隆，测序和表达艾氏杆菌进一步披露的是编码所有9个同源的28-kDa蛋白质基因的多基因位点。重组埃氏杆菌28-kDa蛋白质与来自犬属感染狗的恢复期抗血清反应，并且可用于开发对疾病预防和血清诊断特别有效的疫苗和血清学诊断。

2. 发明授权

US06458942B1 28-kDa immunoreactive protein gene of Ehrlichia canis and uses thereof 失效
标题翻译： 28-kDa Ehrlichia canis的免疫反应蛋白基因及其用途
公开(公告)号：US06458942B1
公开(公告)日：2002-10-01
申请号：US09201458
申请日：1998-11-30
申请人： David H. Walker , Jere W. McBride , Xue-Jie Yu
发明人： David H. Walker , Jere W. McBride , Xue-Jie Yu
IPC分类号： C12N1511
CPC分类号： C07K14/29 , A61K2039/52 , Y02A50/403
摘要： The present invention is directed to the cloning, sequencing and expression of a conserved immunoreactive 28-kDa protein gene (P28) from a polymorphic multiple gene family of Ehrlichia canis. E. canis P28 has an 834-bp open reading frame encoding a protein of 278 amino acids with four variable regions, and shares similar surface-exposed regions, antigenicity and T-cell motifs with E. chaffeensis P28. Also disclosed is that recombinant E. canis P28 protein reacts with convalescent phase antiserum from an E. canis-infected dog.
摘要翻译：本发明涉及从埃氏杆菌的多态性多基因家族克隆，测序和表达保守的免疫反应性28-kDa蛋白质基因（P28）。 E. canis P28具有编码具有四个可变区的278个氨基酸的蛋白质的834bp的开放阅读框，并且与E.Chaffeensis P28共享类似的表面暴露区域，抗原性和T细胞基序。还公开了重组犬E.杆菌P28蛋白与来自犬属感染犬的恢复期抗血清反应。

3. 发明授权

US07344719B2 Homologous 28-kilodalton immunodominant protein genes of Ehrlichia canis and uses thereof 失效
标题翻译： Ehrlichia canis的同源28千道尔顿免疫显性蛋白基因及其用途
公开(公告)号：US07344719B2
公开(公告)日：2008-03-18
申请号：US10731554
申请日：2003-12-09
申请人： David H. Walker , Xue-Jie Yu , Jere W. McBride
发明人： David H. Walker , Xue-Jie Yu , Jere W. McBride
IPC分类号： A61K39/00 , A61K39/02 , C07H21/04 , C12P21/02 , C12N15/09
CPC分类号： C07K14/29 , A61K39/00 , A61K2039/52 , Y02A50/403
摘要： The present invention is directed to the cloning, sequencing and expression of homologous immunoreactive 28-kDa protein genes, p28-1, -2, -3, -5, -6, -7, -9, from a polymorphic multiple gene family of Ehrlichia canis. Further disclosed is a multigene locus encoding all nine homologous 28-kDa protein genes of Ehrlichia canis. Recombinant Ehrlichia canis 28-kDa proteins react with convalescent phase antiserum from an E. canis-infected dog, and may be useful in the development of vaccines and serodiagnostics that are particularly effective for disease prevention and serodiagnosis.
摘要翻译：本发明涉及来自多态性多基因家族的同源免疫反应性28-kDa蛋白基因p28-1，-2，-3，-5，-6，-7，-9的克隆，测序和表达艾氏杆菌进一步披露的是编码所有9个同源的28-kDa蛋白质基因的多基因位点。重组埃氏体28-kDa蛋白质与来自犬属感染狗的恢复期抗血清反应，并且可用于开发对预防疾病和血清诊断特别有效的疫苗和血清学诊断。

4. 发明授权

US06660269B2 Homologous 28-kilodalton immunodominant protein genes of Ehrlichia canis and uses thereof 失效
公开(公告)号：US06660269B2
公开(公告)日：2003-12-09
申请号：US09811007
申请日：2001-03-16
申请人： David H. Walker , Xue-Jie Yu , Jere W. McBride
发明人： David H. Walker , Xue-Jie Yu , Jere W. McBride
IPC分类号： A61K3900
CPC分类号： C07K14/29 , A61K39/00 , A61K2039/52 , Y02A50/403
摘要： The present invention is directed to the cloning, sequencing and expression of homologous immunoreactive 28-kDa protein genes, p28-1, -2, -3, -5, -6, -7, -9, from a polymorphic multiple gene family of Ehrlichia canis. Further disclosed is a multigene locus encoding all nine homologous 28-kDa protein genes of Ehrlichia canis. Recombinant Ehrlichia canis 28-kDa proteins react with convalescent phase antiserum from an E. canis-infected dog, and may be useful in the development of vaccines and serodiagnostics that are particularly effective for disease prevention and serodiagnosis.

5. 发明授权

US06403780B1 Homologous 28-kilodalton immunodominant protein genes of ehrlichia canis and uses thereof 失效
标题翻译：同源28千道尔顿免疫显性蛋白基因的埃及大肠杆菌及其用途
公开(公告)号：US06403780B1
公开(公告)日：2002-06-11
申请号：US09261358
申请日：1999-03-03
申请人： David H. Walker , Xue-Jie Yu , Jere W. McBride
发明人： David H. Walker , Xue-Jie Yu , Jere W. McBride
IPC分类号： C12N1500
CPC分类号： C07K14/29 , A61K2039/52 , Y02A50/403
摘要： The present invention is directed to the cloning, sequencing and expression of homologous immunoreactive 28-kDa protein genes, ECa28-1 and ECa28SA3, from a polymorphic multiple gene family of Ehrlichia canis. A complete sequence of another 28-kDa protein gene, ECaSA2, is also provided. Further disclosed is a multigene locus encoding all five homologous 28-kDa protein genes of Ehrlichia canis. Recombinant Ehrlichia canis 28-kDa proteins react with convalescent phase antiserum from an E. canis-infected dog.
摘要翻译：本发明涉及从埃氏杆菌的多态性多基因家族克隆，测序和表达同源免疫反应性28-kDa蛋白质基因ECa28-1和ECa28SA3。另外提供了另一个28-kDa蛋白质基因ECaSA2的完整序列。进一步公开的是编码埃里希氏菌的所有五个同源的28-kDa蛋白质基因的多基因座。重组埃氏体28-kDa蛋白质与来自犬属感染犬的恢复期抗血清反应。

6. 发明授权

US07556816B2 Ehrlichia chaffeensis 28 kDa outer membrane protein multigene family 失效
公开(公告)号：US07556816B2
公开(公告)日：2009-07-07
申请号：US10284986
申请日：2002-10-31
申请人： David H. Walker , Xue-Jie Yu
发明人： David H. Walker , Xue-Jie Yu
IPC分类号： A61K39/02 , C07K14/295 , C07H21/04 , C12P21/04
CPC分类号： C07K14/29 , A61K39/00 , Y02A50/403
摘要： The 28-kDa outer membrane proteins (P28) of Ehrlichia chaffeensis are encoded by a multigene family consisting of 21 members located in a 23-kb DNA fragment in the genome of E. chaffeensis. Fifteen of these proteins are claimed herein as novel sequences. The amino acid sequence identity of the various P28 proteins was 20-83%. Six of 10 tested p28 genes were actively transcribed in cell culture grown E. chaffeensis. RT-PCR also indicated that each of the p28 genes was monocistronic. These results suggest that the p28 genes are active genes and encode polymorphic forms of the P28 proteins. The P28s were also divergent among different isolates of E. chaffeensis. The large repertoire of the p28 genes in a single ehrlichial organism and antigenic diversity of the P28 among the isolates of E. chaffeensis suggest that the P28s may be involved in immune avoidance.

7. 发明授权

US07332171B2 Ehrlichia chaffeensis 28 kDa outer membrane protein multigene family 失效
标题翻译： Ehrlichia chaffeensis 28 kDa外膜蛋白多基因家族
公开(公告)号：US07332171B2
公开(公告)日：2008-02-19
申请号：US10369293
申请日：2003-02-18
申请人： David H. Walker , Xue-Jie Yu
发明人： David H. Walker , Xue-Jie Yu
IPC分类号： A61K39/02 , C12P21/02 , C07H21/04 , C12N15/09
CPC分类号： C07K14/29 , A61K39/00 , Y02A50/403
摘要： The 28-kDa outer membrane proteins (P28) of Ehrlichia chaffeensis are encoded by a multigene family consisting of 21 members located in a 23-kb DNA fragment in the genome of E. chaffeensis. Fifteen of these proteins are claimed herein as novel sequences. The amino acid sequence identity of the various P28 proteins was 20-83%. Six of 10 tested p28 genes were actively transcribed in cell culture grown E. chaffeensis. RT-PCR also indicated that each of the p28 genes was monocistronic. These results suggest that the p28 genes are active genes and encode polymorphic forms of the P28 proteins. The P28s were also divergent among different isolates of E. chaffeensis. The large repertoire of the p28 genes in a single ehrlichial organism and antigenic diversity of the P28 among the isolates of E. chaffeensis suggest that the P28s may be involved in immune avoidance.
摘要翻译： Ehilichia chaffeensis的28-kDa外膜蛋白（P28）由多基因家族编码，该基因由21个成员组成，位于E.caffeensis基因组的23-kb DNA片段中。这些蛋白质中的十五个被称为新的序列。各种P28蛋白的氨基酸序列同一性为20-83％。 10个测试的p28基因中有6个在生长的E.Chaffeensis的细胞培养物中被主动转录。 RT-PCR也表明每个p28基因是单顺反子。这些结果表明p28基因是活性基因，并编码P28蛋白的多态性。不同分离株之间的P28s也是不同的。大肠杆菌中单核细胞中p28基因的大部分谱系和P28的抗原多样性表明，P28可能参与免疫避免。

8. 发明授权

US06392023B1 Homologous 28-kilodalton immunodominant protein genes of Ehrlicha canis and uses thereof 失效
标题翻译： Ehrlicha canis的同源28千道尔顿免疫显性蛋白基因及其用途
公开(公告)号：US06392023B1
公开(公告)日：2002-05-21
申请号：US09660587
申请日：2000-09-12
申请人： David H. Walker , Xue-Jie Yu , Jere W. McBride
发明人： David H. Walker , Xue-Jie Yu , Jere W. McBride
IPC分类号： C12N1511
CPC分类号： C07K14/29 , A61K39/00 , A61K2039/52 , Y02A50/403
摘要： The present invention is directed to the cloning, sequencing and expression of homologous immunoreactive 28-kDa protein genes, p28-1, -2, -3, -5, -6, -7, -9, from a polymorphic multiple gene family of Ehrlichia canis. Further disclosed is a multigene locus encoding all nine homologous 28-kDa protein genes of Ehrlichia canis. Recombinant Ehrlichia canis 28-kDa proteins react with convalescent phase antiserum from an E. canis-infected dog, and may be useful in the development of vaccines and serodiagnostics that are particularly effective for disease prevention and serodiagnosis.
摘要翻译：本发明涉及来自多态性多基因家族的同源免疫反应性28-kDa蛋白基因p28-1，-2，-3，-5，-6，-7，-9的克隆，测序和表达艾氏杆菌进一步披露的是编码所有9个同源的28-kDa蛋白质基因的多基因位点。重组埃氏体28-kDa蛋白质与来自犬属感染狗的恢复期抗血清反应，并且可用于开发对预防疾病和血清诊断特别有效的疫苗和血清学诊断。

9. 发明授权

US6043085A Ehrlichia canis 120-kDa immunodominant antigenic protein and gene 有权
标题翻译：埃氏杆菌120-kDa免疫显性抗原蛋白和基因
公开(公告)号：US6043085A
公开(公告)日：2000-03-28
申请号：US141047
申请日：1998-08-27
申请人： Xue-Jie Yu , David H. Walker
发明人： Xue-Jie Yu , David H. Walker
IPC分类号： C12N15/09 , A61K38/00 , A61K39/00 , A61K39/02 , A61K48/00 , A61P31/04 , A61P37/04 , C07K14/195 , C07K14/29 , C07K16/12 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/31 , C12N15/85 , C12N15/86 , C12P21/08 , C12R1/01 , C12N15/00 , C12N1/20 , C12N1/14 , C12N1/16
CPC分类号： C07K14/29 , A61K39/00
摘要： The present invention provides a 120-kDa protein gene of Ehrlichia canis, amplified by PCR using primers derived from the DNA sequences flanking the Ehrlichia chaffeensis 120-kDa protein gene. The recombinant E. canis 120-kDa protein contains 14 tandem repeat units with 36 amino acids each. The repeat units are hydrophilic and predicted to be surface-exposed. Also disclosed is that the recombinant E. canis 120-kDa protein is antigenic and reacts with sera from dogs convalescent from canine ehrlichiosis.
摘要翻译：本发明提供了埃里氏杆菌的120-kDa蛋白质基因，通过使用衍生自侧翼于埃里希氏杆菌120-kDa蛋白质基因的DNA序列的引物通过PCR扩增。重组大肠杆菌120-kDa蛋白含有14个串联重复单元，每个具有36个氨基酸。重复单元是亲水性的并且被预测为表面暴露的。还公开的是，重组E.杆菌120-kDa蛋白质是抗原性的，并且与来自犬埃里希氏菌病康复病犬的血清反应。

10. 发明授权

US6015691A Immunodominant 120 kDa surface-exposed adhesion protein genes of Ehrlichia chaffeensis 失效
标题翻译：免疫显性120 kDa表面暴露的埃里希氏杆菌粘附蛋白基因
公开(公告)号：US6015691A
公开(公告)日：2000-01-18
申请号：US656034
申请日：1996-05-31
申请人： David H. Walker , Xue-Jie Yu
发明人： David H. Walker , Xue-Jie Yu
IPC分类号： A61K38/00 , C07K14/29 , C12N1/21 , C12N15/00 , A61K39/02 , C07K14/00 , C12N15/31
CPC分类号： C07K14/29 , A61K38/00
摘要： Disclosed is an isolated gene encoding a 120 kDa immunodominant antigen of Ehrlichia chaffeensis. The 120-kDa protein is one of the immunodominant proteins of E. chaffeensis that stimulates production of specific antibodies in infected humans. Also disclosed are the amino acid sequence of the 120 kDa antigen. Methods of producing a recombinant 120 kDa antigen and therapeutic methods of use of the antigen are also disclosed.
摘要翻译：公开了编码埃里希氏杆菌120kDa免疫显性抗原的分离基因。 120-kDa蛋白是刺激产生感染人体特异性抗体的大肠杆菌的免疫显性蛋白质之一。还公开了120kDa抗原的氨基酸序列。还公开了制备重组120kDa抗原的方法和所述抗原的治疗方法。

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