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1. 发明授权

US07309583B2 Homologous 28-kilodalton immunodominant protein genes of Ehrlichia canis and uses thereof 失效
标题翻译： Ehrlichia canis的同源28千道尔顿免疫显性蛋白基因及其用途
公开(公告)号：US07309583B2
公开(公告)日：2007-12-18
申请号：US10062920
申请日：2002-01-31
申请人： David H. Walker , Xue-Jie Yu , Jere W. McBride
发明人： David H. Walker , Xue-Jie Yu , Jere W. McBride
IPC分类号： C12P21/06 , C07K14/00
CPC分类号： C07K14/29 , A61K39/00 , A61K2039/52 , Y02A50/403
摘要： The present invention is directed to the cloning, sequencing and expression of homologous immunoreactive 28-kDa protein genes, p28-1, -2, -3, -5, -6, -7, -9, from a polymorphic multiple gene family of Ehrlichia canis. Further disclosed is a multigene locus encoding all nine homologous 28-kDa protein genes of Ehrlichia canis. Recombinant Ehrlichia canis 28-kDa proteins react with convalescent phase antiserum from an E. canis-infected dog, and may be useful in the development of vaccines and serodiagnostics that are particularly effective for disease prevention and serodiagnosis.
摘要翻译：本发明涉及来自多态性多基因家族的同源免疫反应性28-kDa蛋白基因p28-1，-2，-3，-5，-6，-7，-9的克隆，测序和表达艾氏杆菌进一步披露的是编码所有9个同源的28-kDa蛋白质基因的多基因位点。重组埃氏杆菌28-kDa蛋白质与来自犬属感染狗的恢复期抗血清反应，并且可用于开发对疾病预防和血清诊断特别有效的疫苗和血清学诊断。

2. 发明授权

US06458942B1 28-kDa immunoreactive protein gene of Ehrlichia canis and uses thereof 失效
标题翻译： 28-kDa Ehrlichia canis的免疫反应蛋白基因及其用途
公开(公告)号：US06458942B1
公开(公告)日：2002-10-01
申请号：US09201458
申请日：1998-11-30
申请人： David H. Walker , Jere W. McBride , Xue-Jie Yu
发明人： David H. Walker , Jere W. McBride , Xue-Jie Yu
IPC分类号： C12N1511
CPC分类号： C07K14/29 , A61K2039/52 , Y02A50/403
摘要： The present invention is directed to the cloning, sequencing and expression of a conserved immunoreactive 28-kDa protein gene (P28) from a polymorphic multiple gene family of Ehrlichia canis. E. canis P28 has an 834-bp open reading frame encoding a protein of 278 amino acids with four variable regions, and shares similar surface-exposed regions, antigenicity and T-cell motifs with E. chaffeensis P28. Also disclosed is that recombinant E. canis P28 protein reacts with convalescent phase antiserum from an E. canis-infected dog.
摘要翻译：本发明涉及从埃氏杆菌的多态性多基因家族克隆，测序和表达保守的免疫反应性28-kDa蛋白质基因（P28）。 E. canis P28具有编码具有四个可变区的278个氨基酸的蛋白质的834bp的开放阅读框，并且与E.Chaffeensis P28共享类似的表面暴露区域，抗原性和T细胞基序。还公开了重组犬E.杆菌P28蛋白与来自犬属感染犬的恢复期抗血清反应。

3. 发明授权

US07344719B2 Homologous 28-kilodalton immunodominant protein genes of Ehrlichia canis and uses thereof 失效
标题翻译： Ehrlichia canis的同源28千道尔顿免疫显性蛋白基因及其用途
公开(公告)号：US07344719B2
公开(公告)日：2008-03-18
申请号：US10731554
申请日：2003-12-09
申请人： David H. Walker , Xue-Jie Yu , Jere W. McBride
发明人： David H. Walker , Xue-Jie Yu , Jere W. McBride
IPC分类号： A61K39/00 , A61K39/02 , C07H21/04 , C12P21/02 , C12N15/09
CPC分类号： C07K14/29 , A61K39/00 , A61K2039/52 , Y02A50/403
摘要： The present invention is directed to the cloning, sequencing and expression of homologous immunoreactive 28-kDa protein genes, p28-1, -2, -3, -5, -6, -7, -9, from a polymorphic multiple gene family of Ehrlichia canis. Further disclosed is a multigene locus encoding all nine homologous 28-kDa protein genes of Ehrlichia canis. Recombinant Ehrlichia canis 28-kDa proteins react with convalescent phase antiserum from an E. canis-infected dog, and may be useful in the development of vaccines and serodiagnostics that are particularly effective for disease prevention and serodiagnosis.
摘要翻译：本发明涉及来自多态性多基因家族的同源免疫反应性28-kDa蛋白基因p28-1，-2，-3，-5，-6，-7，-9的克隆，测序和表达艾氏杆菌进一步披露的是编码所有9个同源的28-kDa蛋白质基因的多基因位点。重组埃氏体28-kDa蛋白质与来自犬属感染狗的恢复期抗血清反应，并且可用于开发对预防疾病和血清诊断特别有效的疫苗和血清学诊断。

4. 发明授权

US06660269B2 Homologous 28-kilodalton immunodominant protein genes of Ehrlichia canis and uses thereof 失效
公开(公告)号：US06660269B2
公开(公告)日：2003-12-09
申请号：US09811007
申请日：2001-03-16
申请人： David H. Walker , Xue-Jie Yu , Jere W. McBride
发明人： David H. Walker , Xue-Jie Yu , Jere W. McBride
IPC分类号： A61K3900
CPC分类号： C07K14/29 , A61K39/00 , A61K2039/52 , Y02A50/403
摘要： The present invention is directed to the cloning, sequencing and expression of homologous immunoreactive 28-kDa protein genes, p28-1, -2, -3, -5, -6, -7, -9, from a polymorphic multiple gene family of Ehrlichia canis. Further disclosed is a multigene locus encoding all nine homologous 28-kDa protein genes of Ehrlichia canis. Recombinant Ehrlichia canis 28-kDa proteins react with convalescent phase antiserum from an E. canis-infected dog, and may be useful in the development of vaccines and serodiagnostics that are particularly effective for disease prevention and serodiagnosis.

5. 发明授权

US06403780B1 Homologous 28-kilodalton immunodominant protein genes of ehrlichia canis and uses thereof 失效
标题翻译：同源28千道尔顿免疫显性蛋白基因的埃及大肠杆菌及其用途
公开(公告)号：US06403780B1
公开(公告)日：2002-06-11
申请号：US09261358
申请日：1999-03-03
申请人： David H. Walker , Xue-Jie Yu , Jere W. McBride
发明人： David H. Walker , Xue-Jie Yu , Jere W. McBride
IPC分类号： C12N1500
CPC分类号： C07K14/29 , A61K2039/52 , Y02A50/403
摘要： The present invention is directed to the cloning, sequencing and expression of homologous immunoreactive 28-kDa protein genes, ECa28-1 and ECa28SA3, from a polymorphic multiple gene family of Ehrlichia canis. A complete sequence of another 28-kDa protein gene, ECaSA2, is also provided. Further disclosed is a multigene locus encoding all five homologous 28-kDa protein genes of Ehrlichia canis. Recombinant Ehrlichia canis 28-kDa proteins react with convalescent phase antiserum from an E. canis-infected dog.
摘要翻译：本发明涉及从埃氏杆菌的多态性多基因家族克隆，测序和表达同源免疫反应性28-kDa蛋白质基因ECa28-1和ECa28SA3。另外提供了另一个28-kDa蛋白质基因ECaSA2的完整序列。进一步公开的是编码埃里希氏菌的所有五个同源的28-kDa蛋白质基因的多基因座。重组埃氏体28-kDa蛋白质与来自犬属感染犬的恢复期抗血清反应。

6. 发明授权

US06392023B1 Homologous 28-kilodalton immunodominant protein genes of Ehrlicha canis and uses thereof 失效
标题翻译： Ehrlicha canis的同源28千道尔顿免疫显性蛋白基因及其用途
公开(公告)号：US06392023B1
公开(公告)日：2002-05-21
申请号：US09660587
申请日：2000-09-12
申请人： David H. Walker , Xue-Jie Yu , Jere W. McBride
发明人： David H. Walker , Xue-Jie Yu , Jere W. McBride
IPC分类号： C12N1511
CPC分类号： C07K14/29 , A61K39/00 , A61K2039/52 , Y02A50/403
摘要： The present invention is directed to the cloning, sequencing and expression of homologous immunoreactive 28-kDa protein genes, p28-1, -2, -3, -5, -6, -7, -9, from a polymorphic multiple gene family of Ehrlichia canis. Further disclosed is a multigene locus encoding all nine homologous 28-kDa protein genes of Ehrlichia canis. Recombinant Ehrlichia canis 28-kDa proteins react with convalescent phase antiserum from an E. canis-infected dog, and may be useful in the development of vaccines and serodiagnostics that are particularly effective for disease prevention and serodiagnosis.
摘要翻译：本发明涉及来自多态性多基因家族的同源免疫反应性28-kDa蛋白基因p28-1，-2，-3，-5，-6，-7，-9的克隆，测序和表达艾氏杆菌进一步披露的是编码所有9个同源的28-kDa蛋白质基因的多基因位点。重组埃氏体28-kDa蛋白质与来自犬属感染狗的恢复期抗血清反应，并且可用于开发对预防疾病和血清诊断特别有效的疫苗和血清学诊断。

7. 发明授权

US07204992B2 P153 and P156 antigens for the immunodiagnosis of canine and human ehrlichioses and uses thereof 有权
标题翻译： P153和P156抗原用于犬和人类ehrlichioses的免疫诊断及其用途
公开(公告)号：US07204992B2
公开(公告)日：2007-04-17
申请号：US10701038
申请日：2003-11-04
申请人： Jere W. McBride , David H. Walker
发明人： Jere W. McBride , David H. Walker
IPC分类号： A61K39/02
CPC分类号： C07H21/04 , A61K39/00 , C07H21/02 , C07K14/29 , Y02A50/403 , Y10S530/81 , Y10S530/82 , Y10S530/825
摘要： Sequences encoding two immunoreactive glycoproteins were cloned from Ehrlichia canis (p153 gene) and Ehrlichia chaffeensis (p156 gene). These two glycoproteins are species-specific immunoreactive orthologs that are useful as subunit vaccines and for serologic and molecular diagnostics for E. canis and E. chaffeensis.
摘要翻译：从埃氏杆菌（p153基因）和埃里希氏梭菌（p156基因）克隆编码两种免疫反应性糖蛋白的序列。这两种糖蛋白是物种特异性的免疫反应性直向同源物，其可用作亚单位疫苗和用于E. canis和E.Chaffeensis的血清学和分子诊断。

8. 发明授权

US07754224B2 P153 and p156 antigens for the immunodiagnosis of canine and human ehrlichioses and uses thereof 有权
标题翻译： P153和p156抗原用于犬和人类ehrlichioses的免疫诊断及其用途
公开(公告)号：US07754224B2
公开(公告)日：2010-07-13
申请号：US11684832
申请日：2007-03-12
申请人： Jere W. McBride , David H. Walker
发明人： Jere W. McBride , David H. Walker
IPC分类号： A61K39/02 , G01N33/53 , C07H21/04 , C07K14/00 , C07K16/00
CPC分类号： C07H21/04 , A61K39/00 , C07H21/02 , C07K14/29 , Y02A50/403 , Y10S530/81 , Y10S530/82 , Y10S530/825
摘要： Sequences encoding two immunoreactive glycoproteins were cloned from Ehrlichia canis (p153 gene) and Ehrlichia chaffeensis (p156 gene). These two glycoproteins are species-specific immunoreactive orthologs that are useful as subunit vaccines and for serologic and molecular diagnostics for E. canis and E. chaffeensis.
摘要翻译：从埃氏杆菌（p153基因）和埃里希氏梭菌（p156基因）克隆编码两种免疫反应性糖蛋白的序列。这两种糖蛋白是物种特异性的免疫反应性直向同源物，其可用作亚单位疫苗和用于E. canis和E.Chaffeensis的血清学和分子诊断。

9. 发明授权

US07722880B2 Immunoreactive 38-KDA ferric binding protein of ehrlichia canis and uses thereof 有权
标题翻译：埃里希氏菌的免疫反应性38-KDA三价铁结合蛋白及其用途
公开(公告)号：US07722880B2
公开(公告)日：2010-05-25
申请号：US11066648
申请日：2005-02-25
申请人： Jere W. McBride , David H. Walker , Christopher Kuyler Doyle
发明人： Jere W. McBride , David H. Walker , Christopher Kuyler Doyle
IPC分类号： A61K39/00 , A61K39/02 , A61K39/38 , A61K45/00 , C12P21/08
CPC分类号： C07K14/29 , A61K38/00 , A61K39/00 , A61K2039/505 , Y02A50/403
摘要： The present invention is directed to the cloning, sequencing, expression, and characterization of an immunoreactive ferric binding protein (Fbp) (38-kDa) protein of Ehrlichia canis encoded by a polynucleotide therefor. In particular embodiments, the protein is employed in an immunogenic composition, such as a vaccine. Methods to induce an immune reaction in an individual with compositions of the invention are provided.
摘要翻译：本发明涉及由其多核苷酸编码的埃立克氏杆菌的免疫反应性铁结合蛋白（Fbp）（38-kDa）蛋白的克隆，测序，表达和表征。在具体实施方案中，蛋白质用于免疫原性组合物，例如疫苗。提供了使用本发明的组合物在个体中诱导免疫反应的方法。

10. 发明授权

US07824692B2 Ehrlichia disulfide bond formation proteins and uses thereof 有权
标题翻译：二硫键形成蛋白及其用途
公开(公告)号：US07824692B2
公开(公告)日：2010-11-02
申请号：US12242865
申请日：2008-09-30
申请人： David H. Walker , Jere W. McBride
发明人： David H. Walker , Jere W. McBride
IPC分类号： A61K39/02 , C12P21/06 , C12P21/04 , C12N15/09 , C07K14/00 , C07H21/02 , C07H21/04
CPC分类号： C12N9/0051 , A61K39/00 , A61K2039/522 , C07K14/29 , Y02A50/403
摘要： Novel genes encoding homologous immunoreactive thio-disulfide oxidoreductases, or disulfide bond formation (Dsb) proteins from Ehrlichia chaffeensis and Ehrlichia canis are disclosed. While the E. chaffeensis and E. canis Dsb proteins are at most only 31% or less homologous to other known Dsb proteins, the Ehrlichia Dsbs contain a cysteine active site, Cys-Gly-Tyr-Cys, similar to those in known Dsb proteins. As predicted by 15-amino acid identical N-terminal signal peptides, the proteins are primarily localized in the periplasm of E. chaffeensis and E. canis, possibly playing a role in antigenicity and pathogenesis. The present invention provides the nucleotide and amino acid sequences and expression vectors for the E. chaffeensis and E. canis dsb genes, antisera directed against the proteins, and kits to determine whether an individual or animal is infected with a given species of Ehrlichia.
摘要翻译：公开了编码同源免疫反应性硫代二硫化物氧化还原酶的新型基因或来自埃里希氏菌和埃氏杆菌的二硫键形成（Dsb）蛋白。虽然E.Chaffeensis和E. canis Dsb蛋白质与其他已知的Dsb蛋白质最多只有31％或更少的同源性，但是Ehrlichia Dsbs含有与已知Dsb蛋白质相似的半胱氨酸活性位点Cys-Gly-Tyr-Cys 。如通过15个氨基酸相同的N末端信号肽所预测的，这些蛋白质主要定位于E.Chaffeensis和E. canis的周质中，可能在抗原性和发病机理中起作用。本发明提供了E.caffeensis和E.canis dsb基因的核苷酸和氨基酸序列和表达载体，针对蛋白质的抗血清，以及用于确定个体或动物是否用给定种类的埃里希菌感染的试剂盒。

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