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    • 6. 发明授权
    • Protection against oxidative stress and inflammation by a cytoprotective response element
    • 通过细胞保护反应元件保护免受氧化应激和炎症
    • US07247714B2
    • 2007-07-24
    • US10271429
    • 2002-10-16
    • Charles KunschSigne E. VarnerXilin ChenJayraz Luchoomun
    • Charles KunschSigne E. VarnerXilin ChenJayraz Luchoomun
    • C07H21/04C07K1/00C12Q1/00C12Q1/68C12N1/20C12N15/00
    • C07K14/4702
    • This invention is in the area of regulatory DNA sequences and their methods of use. Specifically, DNA sequences found in the regulatory region of cytoprotective genes are described that are termed cytoprotective response elements. DNA constructs also are provided that include cytoprotective response elements operably linked to heterologous protein coding sequence, as well as cells and non-human organisms that include the cytoprotective response elements optionally operably linked to a heterologous protein coding sequence, and a method for screening for a compound that increases mRNA or protein regulated by a cytoprotective response element. It has been discovered that the cytoprotective response elements mediate the coordinate activation of certain genes that protect cells from damaging effects of oxidative stress, and that they do so, for example during conditions of hemodynamic shear stress.
    • 本发明在调节DNA序列及其使用方法领域。 具体来说,描述了在细胞保护基因的调节区中发现的称为细胞保护反应元件的DNA序列。 还提供了包括可操作地连接到异源蛋白质编码序列的细胞保护反应元件的DNA构建体,以及包括任选可操作地连接到异源蛋白质编码序列的细胞保护反应元件的细胞和非人生物,以及筛选 增加由细胞保护反应元件调节的mRNA或蛋白质的化合物。 已经发现,细胞保护反应元件介导某些保护细胞免受氧化应激影响的基因的配位活化,例如在血液动力学剪切应力的条件下。