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    • 7. 发明授权
    • Phenylethanol dehydrogenase capable of reducing acetophenone to
R(+)-phenylethanol
    • 将乙炔脱氢酶还原成R(+) - 苯乙醇的苯乙醇脱氢酶
    • US5200335A
    • 1993-04-06
    • US694988
    • 1991-05-06
    • Werner HummelMaria-Regina Kula
    • Werner HummelMaria-Regina Kula
    • C12N9/04C12P7/02C12P7/22C12P7/26C12P41/00C12R1/225
    • C12N9/0006C12P41/00C12P7/02C12P7/22C12P7/26Y10S435/853
    • A phenylethanol dehydrogenase capable of catalyzing the reduction of acetophenone to R(+)-phenylethanol in the presence of NADPH was isolated from Lactobacilli such as Lactobacillus kefir. The dehydrogenase is also capable of catalyzing the reduction of aromatic, alicyclic and aliphatic ketones selected from the group consisting of p-bromoacetophenone, methylcyclohexanone, acetone, methyl hexyl ketone, 4-phenyl-2-butanone, 1-phenyl-1,2-propanedione, ethyl pentyl ketone, pinacolone, propiophenone and p-chloroacetophenone. The dehydrogenase is rapidly inactivated by EDTA, but conventional inhibitors, chelators and SH-protecting reagents have only a slight effect on activity. The enzyme has a K.sub.M of 6.times.10.sup.-4 M for acetophenone. The dehydrogenase is capable of catalyzing the enzymatic reduction of carbonyl compounds to form optically active hydroxy compounds in the presence of NADPH. In such reactions, NADPH can be simultaneously regenerated in the presence of glucose 6-P and glucose-6-P dehydrogenase or isopropanol.
    • 从乳酸杆菌(Lactobacilli)如乳杆菌(Lactobacillus kefir)中分离能够在NADPH存在下催化苯乙酮还原为R(+) - 苯基乙醇的苯乙醇脱氢酶。 脱氢酶还能够催化选自对溴苯乙酮,甲基环己酮,丙酮,甲基己基酮,4-苯基-2-丁酮,1-苯基-1,2-苯并恶唑酮的芳族,脂环族和脂族酮的还原, 丙二酮,乙基戊基酮,频哪酮,苯丙酮和对氯苯乙酮。 脱氢酶由EDTA快速灭活,但是常规的抑制剂,螯合剂和SH-保护试剂对活性只有轻微的影响。 对于苯乙酮,该酶的KM为6×10 -4 M。 在NADPH存在下,脱氢酶能够催化羰基化合物的酶还原形成光学活性羟基化合物。 在这种反应中,NADPH可以在葡萄糖6-P和葡萄糖-6-P脱氢酶或异丙醇的存在下同时再生。
    • 9. 发明授权
    • Fluid friction clutch with actuator
    • 带致动器的流体摩擦离合器
    • US4488628A
    • 1984-12-18
    • US415926
    • 1982-09-08
    • Vlastimil KlunaWerner Hummel
    • Vlastimil KlunaWerner Hummel
    • F16D35/00F16D35/02F16D25/06
    • F16D35/021
    • A fluid friction clutch comprising a rotatable assembly comprising a clutch housing member and a clutch cover member defining a working chamber therein, the working chamber being connected with a working fluid reservoir, valve lever means in said reservoir for controlling the admission of working fluid from the reservoir to the working chamber where it serves to transfer torque between a rotatable disc disposed in the working chamber and said rotatable assembly, means for actuating the valve lever means comprising a pressure chamber formed from a pressure chamber housing and a flexible membrane carrying a pressure plate which directly engages the valve lever means, the pressure chamber housing and membrane being joined to the rotatable assembly to rotate therewith, and means for introducing a pressure medium such as compressed air into said pressure chamber to actuate the valve lever means and control the admission of working fluid from the reservoir into the working chamber, said pressure medium introducing means comprising a connecting fitting pivotably mounted on the pressure chamber housing.
    • 一种流体摩擦离合器,包括可旋转组件,其包括离合器壳体构件和在其中限定工作室的离合器盖构件,所述工作室与工作流体储存器连接,所述储存器中的阀杆装置用于控制工作流体从 工作室的储存器,其用于在设置在工作室中的可旋转盘和所述可旋转组件之间传递扭矩,用于致动阀杆装置的装置包括由压力室壳体形成的压力室和承载压板的柔性膜 其直接接合阀杆装置,压力室壳体和膜连接到可旋转组件以与其一起旋转;以及用于将诸如压缩空气的压力介质引入所述压力室以致动阀杆装置并控制 工作流体从储层进入工作室, 可靠的介质引入装置包括可枢转地安装在压力室壳体上的连接配件。