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1. 发明授权

US06537756B1 Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and Ctenocephalides SPP 有权
公开(公告)号：US06537756B1
公开(公告)日：2003-03-25
申请号：US09611216
申请日：2000-07-06
申请人： Mark J. Rupar , William P. Donovan , Yuping Tan , Annette C. Slaney
发明人： Mark J. Rupar , William P. Donovan , Yuping Tan , Annette C. Slaney
IPC分类号： C12N1532
CPC分类号： A01N63/00 , C07K14/325 , C12N15/8286 , G01N2430/10 , Y02A40/162 , Y10S530/825
摘要： Disclosed is a novel &dgr;-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against coleopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneum). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.

2. 发明授权

US07442540B2 Polynucleotide encoding coleopteran-toxic polypeptide 有权
标题翻译：编码鞘翅目毒性多肽的多核苷酸
公开(公告)号：US07442540B2
公开(公告)日：2008-10-28
申请号：US11485079
申请日：2006-07-12
申请人： Mark J. Rupar , William P. Donovan , Chih-Rei Chu , Elizabeth Pease , Yuping Tan , Annette C. Slaney , Thomas M. Malvar , James A. Baum
发明人： Mark J. Rupar , William P. Donovan , Chih-Rei Chu , Elizabeth Pease , Yuping Tan , Annette C. Slaney , Thomas M. Malvar , James A. Baum
IPC分类号： C12N15/32 , C12N1/21 , C07K14/325 , C12N15/82
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed δ-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.
摘要翻译：公开了新型杀虫多肽，以及包含这些多肽，其肽片段和特异性抗体的组合物。还公开了载体，转化的宿主细胞和含有编码公开的δ-内毒素多肽的核酸片段的转基因植物。还公开了鉴定相关多肽和多核苷酸的方法，制备和使用包含这些多核苷酸序列的转基因细胞的方法，以及用于控制昆虫种群的方法，例如科罗拉多马铃薯甲虫，南方玉米根虫和西方玉米根虫，并赋予对植物对目标昆虫物种的抗性。

3. 发明授权

US07186893B2 Plants transformed with CryET29-encoding nucleic acids 有权
标题翻译：用CryET29编码核酸转化的植物
公开(公告)号：US07186893B2
公开(公告)日：2007-03-06
申请号：US10767605
申请日：2004-01-29
申请人： Mark J. Rupar , William P. Donovan , Yuping Tan , Annette C. Slaney
发明人： Mark J. Rupar , William P. Donovan , Yuping Tan , Annette C. Slaney
IPC分类号： A01H5/00 , A01H5/10 , C12N5/04
CPC分类号： A01N63/00 , C07K14/325 , C12N15/8286 , G01N2430/10 , Y02A40/162 , Y10S530/825
摘要： Disclosed are transgenic plants, seeds, and progeny from such plants comprising a cryET29 DNA segment. The cryET29 DNA segment encodes CryET29 crystal protein which comprises SEQ ID NO:2 and exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against coleopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneum).
摘要翻译：公开了包含cryET29 DNA片段的来自这种植物的转基因植物，种子和后代。 cryET29 DNA区段编码CryET29晶体蛋白，其包含SEQ ID NO：2，并且显示出对iphonapteran昆虫的杀虫活性，包括猫跳蚤幼虫（Ctenocephalides felis）以及鞘翅目昆虫，包括南方玉米根虫（Diabrotica undecimpunctata），西方玉米根虫（D. virgifera），科罗拉多马铃薯甲虫（Leptinotarsa decemlineata），日本甲虫（Popillia japonica）和红粉甲虫（Tribolium castaneum））。

4. 发明授权

US5759538A Bacillus thuringiensis apr and npr genes, apr and npr B.t. strains, and method of use 失效
标题翻译：苏云金芽孢杆菌apr和npr基因，apr和npr B.t. 菌株和使用方法
公开(公告)号：US5759538A
公开(公告)日：1998-06-02
申请号：US415823
申请日：1995-03-31
申请人： William P. Donovan , Yuping Tan
发明人： William P. Donovan , Yuping Tan
IPC分类号： A01N63/00 , C07K14/325 , C12N1/21 , C12N9/54 , C12N15/57 , C12N1/20
CPC分类号： C07K14/325 , A01N63/00 , C12N9/54
摘要： The present invention is based on the discovery, isolation, sequencing and characterization of two protease genes present in Bacillus thuringiensis (B.t.). One is an alkaline protease gene generally designated "apr. " The other is a neutral protease gene generally designated "npr." The invention also includes genetically disabling the apr and the npr genes to reduce proteolytic activity of the proteases encoded by the genes, and using the disabled genes in insecticidal B.t. constructs along with one or more B.t. protein toxin genes. These resulting B.t. constructs are useful for the enhanced production of B.t. toxin protein. The B.t. toxin protein recovered from the fermentation production of these B.t. constructs also exhibits improved stability with respect to its insecticidal activity.
摘要翻译：本发明基于存在于苏云金芽孢杆菌（B.t.）中的两种蛋白酶基因的发现，分离，测序和表征。一种是通常称为“apr”的碱性蛋白酶基因。另一种是通常称为“npr”的中性蛋白酶基因。本发明还包括遗传地使apr和npr基因失活以降低由该基因编码的蛋白酶的蛋白水解活性，并且在杀虫剂B.t.中使用残基的基因。与一个或多个B.t.一起构建。蛋白毒素基因。这些结果构建体可用于增强生产B.t. 毒素蛋白。 B.t. 从这些B.t.的发酵生产中回收的毒素蛋白质。相对于其杀虫活性，构建体还表现出改进的稳定性。

5. 发明授权

US07572587B2 Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and Ctenocephalides spp 失效
标题翻译：苏云金芽孢杆菌CryET29组合物对鞘翅目昆虫和栉头菜属毒素有毒
公开(公告)号：US07572587B2
公开(公告)日：2009-08-11
申请号：US11712704
申请日：2007-03-01
申请人： Mark J. Rupar , William P. Donovan , Yuping Tan , Annette C. Slaney
发明人： Mark J. Rupar , William P. Donovan , Yuping Tan , Annette C. Slaney
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： A01N63/00 , C07K14/325 , C12N15/8286 , G01N2430/10 , Y02A40/162 , Y10S530/825
摘要： Disclosed is a novel δ-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against coleopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneum). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.
摘要翻译：公开了一种名为CryET29的新型三角内含物，其表现出对iphonapteran昆虫具有杀虫活性，包括猫跳蚤幼虫（Ctenocephalides felis）以及鞘翅目昆虫，包括南方玉米根虫（Diabrotica undecimpunctata），西方玉米根虫（D. virgifera），科罗拉多马铃薯甲虫（Leptinotarsa decemlineata），日本甲虫（Popillia japonica）和红粉甲虫（Tribolium castaneum））。还公开了编码CryET29，重组载体，宿主细胞和包含cryET29 DNA片段的转基因植物的核酸片段。公开了制备和使用所公开的蛋白质和核酸片段的方法以及用于在体内和体外检测cryET29和CryET29序列的测定和诊断试剂盒。

6. 发明授权

US6093695A Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and ctenocephalides SPP 失效
标题翻译：苏云金芽孢杆菌CryET29组合物对鞘翅目昆虫和c螨素SPP有毒
公开(公告)号：US6093695A
公开(公告)日：2000-07-25
申请号：US721259
申请日：1996-09-26
申请人： Mark J. Rupar , William P. Donovan , Yuping Tan , Annette C. Slaney
发明人： Mark J. Rupar , William P. Donovan , Yuping Tan , Annette C. Slaney
IPC分类号： C12N15/09 , A01N63/02 , C07K14/325 , C12N1/21 , C12N5/10 , C12N15/32 , C12Q1/68 , C12R1/19 , C12N1/20
CPC分类号： A01N63/00 , C07K14/325 , C12N15/8286 , G01N2430/10 , Y10S530/825
摘要： Disclosed is a novel .delta.-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against colcopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneur). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.
摘要翻译：公开了一种名为CryET29的新颖的内含肽，其表现出对iphonapteran昆虫具有杀虫活性，包括猫蚤（Ctenocephalides felis）的幼虫，以及对鳞翅目昆虫，包括南方玉米根虫（Diabrotica undecimpunctata），西方玉米根虫（D. virgifera），科罗拉多马铃薯甲虫（Leptinotarsa decemlineata），日本甲虫（Popillia japonica）和红粉甲虫（Tribolium castaneur）。还公开了编码CryET29，重组载体，宿主细胞和包含cryET29 DNA片段的转基因植物的核酸片段。公开了制备和使用所公开的蛋白质和核酸片段的方法以及用于在体内和体外检测cryET29和CryET29序列的测定和诊断试剂盒。

7. 发明授权

US5679343A Bacillus thuringiensis cryET4 and cryET5 protein insecticidal composition and method of use 失效
标题翻译：苏云金芽孢杆菌cryET4和cryET5蛋白杀虫组合物及其使用方法
公开(公告)号：US5679343A
公开(公告)日：1997-10-21
申请号：US474038
申请日：1995-06-07
申请人： William P. Donovan , Yuping Tan , Christine S. Jany , Jose M. Gonzalez, Jr.
发明人： William P. Donovan , Yuping Tan , Christine S. Jany , Jose M. Gonzalez, Jr.
IPC分类号： C07K14/32 , C07K14/325 , C12N1/21 , C12N15/32 , A01N63/00 , A01N63/02
CPC分类号： C07K14/32 , C07K14/325 , C12R1/075 , Y10S435/832
摘要： A Bacillus thuringiensis strain isolate, designated B.t. strain EG5847, exhibits insecticidal activity against lepidopteran insects. Two novel toxin genes from B.t. strain EG5847 designated cryET4 and cryET5 produce insecticidal proteins with activity against a broad spectrum of insects of the order Lepidoptera. The cryET4 gene has a nucleotide base sequence shown in FIG. 1 and listed in SEQ ID NO:1 and produces a CryET4 gene product having the deduced amino acid sequence shown in FIG. 1 and listed in SEQ ID NO:2. The cryET5 gene has a nucleotide base sequence shown in FIG. 2 and listed in SEQ ID NO:3 and produces a CryET5 gene product having the deduced amino acid sequence shown in FIG. 2 and listed in SEQ ID NO:4.
摘要翻译：苏云金芽孢杆菌菌株分离株，命名为B.t. 菌株EG5847对鳞翅目昆虫表现出杀虫活性。来自B.t.的两种新型毒素基因命名为cryET4和cryET5的菌株EG5847产生具有针对鳞翅目鳞翅目昆虫的活性的杀虫蛋白。 cryET4基因具有图1所示的核苷酸碱基序列。 1并列于SEQ ID NO：1中，并产生具有如图1所示的推导的氨基酸序列的CryET4基因产物。 1并列于SEQ ID NO：2。 cryET5基因具有图1所示的核苷酸碱基序列。 2并列于SEQ ID NO：3中，并产生具有如图1所示的推导的氨基酸序列的CryET5基因产物。 2并列于SEQ ID NO：4。

8. 发明授权

US5322687A Bacillus thuringiensis cryet4 and cryet5 toxin genes and proteins toxic to lepidopteran insects 失效
标题翻译：苏云金芽孢杆菌cryet4和cryet5毒素基因和对鳞翅目昆虫有毒的蛋白质
公开(公告)号：US5322687A
公开(公告)日：1994-06-21
申请号：US100709
申请日：1993-07-29
申请人： William P. Donovan , Yuping Tan , Christine S. Jany , Jose M. Gonzalez, Jr.
发明人： William P. Donovan , Yuping Tan , Christine S. Jany , Jose M. Gonzalez, Jr.
IPC分类号： C07K14/32 , C07K14/325 , C12N1/21 , C12N15/32 , A61K45/00 , C12N15/75
CPC分类号： C07K14/32 , C07K14/325 , C12R1/075 , Y10S435/832
摘要： A Bacillus thuringiensis strain isolate, designated B.t. strain EG5847, exhibits insecticidal activity against lepidopteran insects. Two novel toxin genes from B.t. strain EG5847 designated cryET4 and cryET5 produce insecticidal proteins with activity against a broad spectrum of insects of the order Lepidoptera. The cryET4 gene has a nucleotide base sequence shown in FIG. 1 and listed in SEQ ID NO:1 and produces a CryET4 gene product having the deduced amino acid sequence shown in FIG. 1 and listed in SEQ ID NO:2. The cryET5 gene has a nucleotide base sequence shown in FIG. 2 and listed in SEQ ID NO:3 and produces a CryET5 gene product having the deduced amino acid sequence shown in FIG. 2 and listed in SEQ ID NO:4.
摘要翻译：苏云金芽孢杆菌菌株分离株，命名为B.t. 菌株EG5847对鳞翅目昆虫表现出杀虫活性。来自B.t.的两种新型毒素基因命名为cryET4和cryET5的菌株EG5847产生具有针对鳞翅目鳞翅目昆虫的活性的杀虫蛋白。 cryET4基因具有图1所示的核苷酸碱基序列。 1并列于SEQ ID NO：1中，并产生具有如图1所示的推导的氨基酸序列的CryET4基因产物。 1并列于SEQ ID NO：2。 cryET5基因具有图1所示的核苷酸碱基序列。 2并列于SEQ ID NO：3中，并产生具有如图1所示的推导的氨基酸序列的CryET5基因产物。 2并列于SEQ ID NO：4。

9. 发明授权

US07772369B2 Coleopteran-toxic polypeptide compositions and insect-resistant transgenic plants 有权
标题翻译：鞘翅目毒性多肽组合物和抗虫转基因植物
公开(公告)号：US07772369B2
公开(公告)日：2010-08-10
申请号：US12256812
申请日：2008-10-23
申请人： Mark J. Rupar , William P. Donovan , Chih-Rei Chu , Elizabeth Pease , Yuping Tan , Annette C. Slaney , Thomas M. Malvar , James A. Baum
发明人： Mark J. Rupar , William P. Donovan , Chih-Rei Chu , Elizabeth Pease , Yuping Tan , Annette C. Slaney , Thomas M. Malvar , James A. Baum
IPC分类号： C07K14/325
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed δ-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.
摘要翻译：公开了新型杀虫多肽，以及包含这些多肽，其肽片段和特异性抗体的组合物。还公开了载体，转化的宿主细胞和含有编码所公开的δ-内毒素多肽的核酸片段的转基因植物。还公开了鉴定相关多肽和多核苷酸的方法，制备和使用包含这些多核苷酸序列的转基因细胞的方法，以及用于控制昆虫种群的方法，例如科罗拉多马铃薯甲虫，南方玉米根虫和西方玉米根虫，并赋予对植物对目标昆虫物种的抗性。

10. 发明申请

US20090094714A1 Coleopteran-Toxic Polypeptide Compositions And Insect-Resistant Transgenic Plants 有权
标题翻译：鞘翅目有毒多肽组合物和抗虫转基因植物
公开(公告)号：US20090094714A1
公开(公告)日：2009-04-09
申请号：US12256812
申请日：2008-10-23
申请人： Mark J. Rupar , William P. Donovan , Chih-Rei Chu , Elizabeth Pease , Yuping Tan , Annette C. Slaney , Thomas M. Malvar , James A. Baum
发明人： Mark J. Rupar , William P. Donovan , Chih-Rei Chu , Elizabeth Pease , Yuping Tan , Annette C. Slaney , Thomas M. Malvar , James A. Baum
IPC分类号： A01H1/00 , C07K7/08 , C07K14/00 , C12N1/20 , C12P21/06 , C07H21/04 , C12Q1/68 , C12N15/63 , C12N5/10 , A01H5/00
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed δ-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.
摘要翻译：公开了新型杀虫多肽，以及包含这些多肽，其肽片段和特异性抗体的组合物。还公开了载体，转化的宿主细胞和含有编码公开的δ-内毒素多肽的核酸片段的转基因植物。还公开了鉴定相关多肽和多核苷酸的方法，制备和使用包含这些多核苷酸序列的转基因细胞的方法，以及用于控制昆虫种群的方法，例如科罗拉多马铃薯甲虫，南方玉米根虫和西方玉米根虫，并赋予对植物对目标昆虫物种的抗性。

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