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1. 发明授权

US5942658A Transformed plant with Bacillus thuringiensis toxin gene 失效
标题翻译：转化植物与苏云金芽孢杆菌毒素基因
公开(公告)号：US5942658A
公开(公告)日：1999-08-24
申请号：US881340
申请日：1997-06-24
申请人： William P. Donovan , Yuping Tan , Christine S. Jany , Jose M. Gonzalez, Jr.
发明人： William P. Donovan , Yuping Tan , Christine S. Jany , Jose M. Gonzalez, Jr.
IPC分类号： C07K14/32 , C07K14/325 , C12N1/21 , C12N15/32 , A01H5/00 , C12N5/10
CPC分类号： C07K14/32 , C07K14/325 , C12R1/075 , Y10S435/832
摘要： A Bacillus thuringiensis strain isolate, designated B.t. strain EG5847, exhibits insecticidal activity against lepidopteran insects. Two novel toxin genes from B.t. strain EG5847 designated cryET4 and cryET5 produce insecticidal proteins with activity against a broad spectrum of insects of the order Lepidoptera, The cryET4 gene has a nucleotide base sequence shown in FIG. 1 and listed in SEQ ID NO:1 and produces a CryET4 gene product having the deduced amino acid sequence shown in FIG. 1 and listed in SEQ ID NO:2. The cryET5 gene has a nucleotide base sequence shown in FIG. 2 and listed in SEQ ID NO:3 and produces a CryET5 gene product having the deduced amino acid sequence shown in FIG. 2 and listed in SEQ ID NO:4.
摘要翻译：苏云金芽孢杆菌菌株分离株，命名为B.t. 菌株EG5847对鳞翅目昆虫表现出杀虫活性。来自B.t.的两种新型毒素基因命名为cryET4和cryET5的菌株EG5847产生具有针对鳞翅目广谱昆虫的活性的杀虫蛋白，cryET4基因具有图1所示的核苷酸碱基序列。 1并列于SEQ ID NO：1中，并产生具有如图1所示的推导的氨基酸序列的CryET4基因产物。 1并列于SEQ ID NO：2。 cryET5基因具有图1所示的核苷酸碱基序列。 2并列于SEQ ID NO：3中，并产生具有如图1所示的推导的氨基酸序列的CryET5基因产物。 2并列于SEQ ID NO：4。

2. 发明授权

US5679343A Bacillus thuringiensis cryET4 and cryET5 protein insecticidal composition and method of use 失效
标题翻译：苏云金芽孢杆菌cryET4和cryET5蛋白杀虫组合物及其使用方法
公开(公告)号：US5679343A
公开(公告)日：1997-10-21
申请号：US474038
申请日：1995-06-07
申请人： William P. Donovan , Yuping Tan , Christine S. Jany , Jose M. Gonzalez, Jr.
发明人： William P. Donovan , Yuping Tan , Christine S. Jany , Jose M. Gonzalez, Jr.
IPC分类号： C07K14/32 , C07K14/325 , C12N1/21 , C12N15/32 , A01N63/00 , A01N63/02
CPC分类号： C07K14/32 , C07K14/325 , C12R1/075 , Y10S435/832
摘要： A Bacillus thuringiensis strain isolate, designated B.t. strain EG5847, exhibits insecticidal activity against lepidopteran insects. Two novel toxin genes from B.t. strain EG5847 designated cryET4 and cryET5 produce insecticidal proteins with activity against a broad spectrum of insects of the order Lepidoptera. The cryET4 gene has a nucleotide base sequence shown in FIG. 1 and listed in SEQ ID NO:1 and produces a CryET4 gene product having the deduced amino acid sequence shown in FIG. 1 and listed in SEQ ID NO:2. The cryET5 gene has a nucleotide base sequence shown in FIG. 2 and listed in SEQ ID NO:3 and produces a CryET5 gene product having the deduced amino acid sequence shown in FIG. 2 and listed in SEQ ID NO:4.
摘要翻译：苏云金芽孢杆菌菌株分离株，命名为B.t. 菌株EG5847对鳞翅目昆虫表现出杀虫活性。来自B.t.的两种新型毒素基因命名为cryET4和cryET5的菌株EG5847产生具有针对鳞翅目鳞翅目昆虫的活性的杀虫蛋白。 cryET4基因具有图1所示的核苷酸碱基序列。 1并列于SEQ ID NO：1中，并产生具有如图1所示的推导的氨基酸序列的CryET4基因产物。 1并列于SEQ ID NO：2。 cryET5基因具有图1所示的核苷酸碱基序列。 2并列于SEQ ID NO：3中，并产生具有如图1所示的推导的氨基酸序列的CryET5基因产物。 2并列于SEQ ID NO：4。

3. 发明授权

US5322687A Bacillus thuringiensis cryet4 and cryet5 toxin genes and proteins toxic to lepidopteran insects 失效
标题翻译：苏云金芽孢杆菌cryet4和cryet5毒素基因和对鳞翅目昆虫有毒的蛋白质
公开(公告)号：US5322687A
公开(公告)日：1994-06-21
申请号：US100709
申请日：1993-07-29
申请人： William P. Donovan , Yuping Tan , Christine S. Jany , Jose M. Gonzalez, Jr.
发明人： William P. Donovan , Yuping Tan , Christine S. Jany , Jose M. Gonzalez, Jr.
IPC分类号： C07K14/32 , C07K14/325 , C12N1/21 , C12N15/32 , A61K45/00 , C12N15/75
CPC分类号： C07K14/32 , C07K14/325 , C12R1/075 , Y10S435/832
摘要： A Bacillus thuringiensis strain isolate, designated B.t. strain EG5847, exhibits insecticidal activity against lepidopteran insects. Two novel toxin genes from B.t. strain EG5847 designated cryET4 and cryET5 produce insecticidal proteins with activity against a broad spectrum of insects of the order Lepidoptera. The cryET4 gene has a nucleotide base sequence shown in FIG. 1 and listed in SEQ ID NO:1 and produces a CryET4 gene product having the deduced amino acid sequence shown in FIG. 1 and listed in SEQ ID NO:2. The cryET5 gene has a nucleotide base sequence shown in FIG. 2 and listed in SEQ ID NO:3 and produces a CryET5 gene product having the deduced amino acid sequence shown in FIG. 2 and listed in SEQ ID NO:4.
摘要翻译：苏云金芽孢杆菌菌株分离株，命名为B.t. 菌株EG5847对鳞翅目昆虫表现出杀虫活性。来自B.t.的两种新型毒素基因命名为cryET4和cryET5的菌株EG5847产生具有针对鳞翅目鳞翅目昆虫的活性的杀虫蛋白。 cryET4基因具有图1所示的核苷酸碱基序列。 1并列于SEQ ID NO：1中，并产生具有如图1所示的推导的氨基酸序列的CryET4基因产物。 1并列于SEQ ID NO：2。 cryET5基因具有图1所示的核苷酸碱基序列。 2并列于SEQ ID NO：3中，并产生具有如图1所示的推导的氨基酸序列的CryET5基因产物。 2并列于SEQ ID NO：4。

4. 发明授权

US5854053A Bacillus thuringiensis bacteria 失效
标题翻译：苏云金芽孢杆菌细菌
公开(公告)号：US5854053A
公开(公告)日：1998-12-29
申请号：US779046
申请日：1997-01-06
申请人： William P. Donovan , Jose M. Gonzalez, Jr.
发明人： William P. Donovan , Jose M. Gonzalez, Jr.
IPC分类号： C07K14/32 , C07K14/325 , C12N1/21 , C12N15/32 , C12N1/20 , A01N63/00
CPC分类号： C07K14/32 , C07K14/325 , C12R1/075 , Y10S435/832
摘要： A Bacillus thuringiensis strain isolate, designated B.t. strain EG5847, exhibits insecticidal activity against lepidopteran insects. Two novel toxin genes from B.t. strain EG5847 designated cryET4 and cryET5 produce insecticidal proteins with activity against a broad spectrum of insects of the order Lepidoptera. The cryET4 gene has a nucleotide base sequence shown in FIG. 1 and listed in SEQ ID NO:1 and produces a CryET4 gene product having the deduced amino acid sequence shown in FIG. 1 and listed in SEQ ID NO:2. The cryET5 gene has a nucleotide base sequence shown in FIG. 2 and listed in SEQ ID NO:3 and produces a CryET5 gene product having the deduced amino acid sequence shown in FIG. 2 and listed in SEQ ID NO:4.
摘要翻译：苏云金芽孢杆菌菌株分离株，命名为B.t. 菌株EG5847对鳞翅目昆虫表现出杀虫活性。来自B.t.的两种新型毒素基因命名为cryET4和cryET5的菌株EG5847产生具有针对鳞翅目鳞翅目昆虫的活性的杀虫蛋白。 cryET4基因具有图1所示的核苷酸碱基序列。 1并列于SEQ ID NO：1中，并产生具有如图1所示的推导的氨基酸序列的CryET4基因产物。 1并列于SEQ ID NO：2。 cryET5基因具有图1所示的核苷酸碱基序列。 2并列于SEQ ID NO：3中，并产生具有如图1所示的推导的氨基酸序列的CryET5基因产物。 2并列于SEQ ID NO：4。

5. 发明授权

US5024837A Coleopteran active microorganisms, related insecticide compositions and methods for their production and use 失效
标题翻译：鞘翅目活性微生物，相关杀虫剂组合物及其生产和使用的方法
公开(公告)号：US5024837A
公开(公告)日：1991-06-18
申请号：US47945
申请日：1987-05-06
申请人： William P. Donovan , Jose M. Gonzalez, Jr. , Barry L. Levinson , Anthony Macaluso
发明人： William P. Donovan , Jose M. Gonzalez, Jr. , Barry L. Levinson , Anthony Macaluso
IPC分类号： A01H5/00 , A01N63/00 , C07K14/195 , C07K14/325 , C07K14/41 , C12N1/21 , C12N15/09 , C12P21/02 , C12Q1/68 , C12R1/07 , C12R1/19
CPC分类号： C07K14/325 , A01N63/00
摘要： This invention relates to Bacillus thuringiensis strains that have insecticidal activity against lepidopteran and coleopteran insects, the coleopteran-active endotoxin being produced by an acquired plasmid. This invention also relates to the crystalline protein toxin useful as a biological insecticide against Coleoptera which toxin is produced by the strain of Bacillus thuringiensis. This invention also relates to the expression in various microorganisms of the gene, known as cryC, which codes for this toxin, and for related novel insecticide compositions and methods for their use.
摘要翻译：本发明涉及对鳞翅目昆虫和鞘翅目昆虫具有杀虫活性的苏云金芽孢杆菌属菌种，由获得性质粒产生的鞘翅目昆虫活性内毒素。本发明还涉及可用作针对鞘翅目的生物杀虫剂的结晶蛋白毒素，该毒素是由苏云金芽孢杆菌菌株产生的。本发明还涉及编码该毒素的已知cryC的基因的各种微生物的表达，以及相关新型杀虫剂组合物及其用途的方法。

6. 发明授权

US5147640A Strains of Bacillus thuringiensis insecticidal compositions containing the same 失效
标题翻译：含有其的BACILLUS THURINGIENSIS INSECTICIDAL组合物的菌株
公开(公告)号：US5147640A
公开(公告)日：1992-09-15
申请号：US268307
申请日：1988-11-07
申请人： Ivan E. Gard, Jr. , Jose M. Gonzalez, Jr. , Dennis R. Ciarlante
发明人： Ivan E. Gard, Jr. , Jose M. Gonzalez, Jr. , Dennis R. Ciarlante
IPC分类号： A01N63/02 , C07K14/325 , C12N1/21 , C12N15/32 , C12P21/00 , C12R1/07
CPC分类号： C12R1/075 , C07K14/325
摘要： The present invention provides novel strains of Bacillus thuringiensis containing genes that code for P1 endotoxin proteins. The invention also provides methods and compositions for controlling insects or protecting plants from insect attack with a novel strain of Bacillus thuringiensis.
摘要翻译：本发明提供了含有编码P1内毒素蛋白的基因的苏云金芽孢杆菌新菌株。本发明还提供了用新型苏云金芽孢杆菌菌株控制昆虫或保护植物免受昆虫攻击的方法和组合物。

7. 发明授权

US5080897A Novel bacillus thuringiensis strains, and related insecticidal compositions 失效
标题翻译：新颖的BACILLUS THURINGIENSIS菌株和相关的杀虫剂组合物
公开(公告)号：US5080897A
公开(公告)日：1992-01-14
申请号：US185613
申请日：1988-04-25
申请人： Jose M. Gonzalez, Jr. , Anthony Macaluso
发明人： Jose M. Gonzalez, Jr. , Anthony Macaluso
IPC分类号： C12N15/09 , A01N63/00 , C07K14/325 , C12N1/21 , C12R1/07
CPC分类号： C12R1/075 , C07K14/325
摘要： The present invention relates to novel Bacillus thuringiensis transconjugant strains having improved insecticidal activity. The novel strains are produced by a combination of plasmid curing and conjugation of active strains.
摘要翻译：本发明涉及具有改善的杀虫活性的新型苏云金芽孢杆菌转导结合菌株。新菌株通过活性菌株的质粒固化和缀合的组合产生。

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