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1. 发明申请

US20050050588A1 Systemic small RNA signaling system in plants 失效
标题翻译：系统小RNA信号系统在植物中
公开(公告)号：US20050050588A1
公开(公告)日：2005-03-03
申请号：US10871841
申请日：2004-06-17
申请人： William Lucas , Byung-Chun Yoo , Tony Lough , Erika Varkonyi-Gasic
发明人： William Lucas , Byung-Chun Yoo , Tony Lough , Erika Varkonyi-Gasic
IPC分类号： A01H1/00 , C07H21/04 , C07K14/415 , C12N5/04 , C12N9/22 , C12N15/82 , C12Q20060101
CPC分类号： C07K14/415 , C12N15/8261 , Y02A40/146
摘要： Systemic translocation of RNA exerts non-cell-autonomous epigenetic control over plant development and defense. The present invention provides a polynucleotide sequence encoding a component of the protein machinery involved in small RNA trafficking, Cucurbita maxima PHLOEM SMALL RNA-BINDING PROTEIN1 (CmPSRB1), and the corresponding polypeptide sequence. Homologs of the disclosed sequences exist in other plant species. The invention also provides polynucleotide sequences of phloem small RNAs involved in signaling. Methods are also provided for using these PSRB1 and small RNA sequences and genetic constructs comprising these sequences to alter (e.g., prevent, reduce or elevate) non-cell autonomous signaling events in plants involving small RNA metabolism. These signaling events are involved in a broad spectrum of plant physiological and biochemical processes, including, for example, flowering, coordination of plant growth and development, systemic resistance to pathogens, responses to environmental stresses, e.g., heat, drought, salinity, systemic gene silencing (e.g., viral infections), biomass distribution, regulation of carbon metabolism and control of plant size and developmental timing and patterning.
摘要翻译： RNA的系统易位对植物发育和防御发挥非细胞自主的表观遗传控制。本发明提供了编码涉及小RNA运输的蛋白质机械的组分的多核苷酸序列，南瓜（Cucurbita maxima）PHLOEM SMALL RNA-BINDING PROTEIN1（CmPSRB1）以及相应的多肽序列。所公开序列的同源物存在于其他植物物种中。本发明还提供涉及信号传导的韧皮部小RNA的多核苷酸序列。还提供了使用这些PSRB1和小RNA序列以及包含这些序列的遗传构建体来改变（例如，预防，减少或提高）涉及小RNA代谢的植物中的非细胞自主信号传导事件的方法。这些信号事件涉及广泛的植物生理和生物化学过程，包括例如开花，植物生长和发育的协调，对病原体的系统抗性，对环境胁迫的反应，例如热，干旱，盐度，全身基因沉默（例如病毒感染），生物量分布，碳代谢调节和植物大小控制以及发育时间和图案化。

2. 发明申请

US20070079401A1 Control of floral induction 审中-公开
标题翻译：控制花卉诱导
公开(公告)号：US20070079401A1
公开(公告)日：2007-04-05
申请号：US11528043
申请日：2006-09-26
申请人： Tony Lough , Dieter Hermsmeier , Erika Varkonyi-Gasic , Justin Sweetman , Ilkka Havukkala , Helene Belanger , Richard Forster , Keith Hudson , William Lucas
发明人： Tony Lough , Dieter Hermsmeier , Erika Varkonyi-Gasic , Justin Sweetman , Ilkka Havukkala , Helene Belanger , Richard Forster , Keith Hudson , William Lucas
IPC分类号： A01H1/00 , C07H21/04 , C12N9/24 , C12N15/82 , C12N5/04
CPC分类号： C07K14/415 , C12N15/827
摘要： The present application discloses plant polynucleotides, their encoded polypeptide sequences, and sRNA sequences which are putative regulators of long-distance florigenic signaling and flowering control. Methods of use of these sequences related to long-distance florigenic signaling are also disclosed.
摘要翻译：本申请公开了植物多核苷酸，其编码的多肽序列和作为长距离发花信号和开花控制的假定调节子的sRNA序列。还公开了与长距离发花信号相关的这些序列的使用方法。

3. 发明授权

US07189892B2 Nucleic acids encoding phloem small RNA-binding proteins and transgenic plants comprising them 失效
标题翻译：编码韧皮部小RNA结合蛋白的核酸和包含它们的转基因植物
公开(公告)号：US07189892B2
公开(公告)日：2007-03-13
申请号：US10871841
申请日：2004-06-17
申请人： William J. Lucas , Byung-Chun Yoo , Tony J. Lough , Erika Varkonyi-Gasic
发明人： William J. Lucas , Byung-Chun Yoo , Tony J. Lough , Erika Varkonyi-Gasic
IPC分类号： C12N15/09 , C12N15/29 , C12N15/82 , A01H5/00
CPC分类号： C07K14/415 , C12N15/8261 , Y02A40/146
摘要： The present invention provides a polynucleotide sequence encoding a component of the protein machinery involved in small RNA trafficking, Cucurbita maxima phloem small RNA-binding protein (CmPSRB 1), and the corresponding polypeptide sequence. The invention also provides genetic constructs and transgenic plants comprising the polynucleotide sequence encoding a phloem small RNA-binding protein to alter (e.g., prevent, reduce or elevate) non-cell autonomous signaling events in the plants involving small RNA metabolism. These signaling events are involved in a broad spectrum of plant physiological and biochemical processes, including, for example, systemic resistance to pathogens, responses to environmental stresses, e.g., heat, drought, salinity, and systemic gene silencing (e.g., viral infections).
摘要翻译：本发明提供了编码涉及小RNA运输的蛋白质机械的组分的多核苷酸序列，南美黄瓜韧皮部小RNA结合蛋白（CmPSRB1）以及相应的多肽序列。本发明还提供遗传构建体和转基因植物，其包含编码韧皮部小RNA结合蛋白的多核苷酸序列，以改变（例如，预防，降低或升高）涉及小RNA代谢的植物中的非细胞自发性信号传导事件。这些信号事件涉及广泛的植物生理和生化过程，包括例如对病原体的系统抗性，对环境胁迫的反应，例如热，干旱，盐度和全身基因沉默（例如，病毒感染）。

4. 发明授权

US07071380B1 Control of floral induction 失效
标题翻译：控制花卉诱导
公开(公告)号：US07071380B1
公开(公告)日：2006-07-04
申请号：US10931081
申请日：2004-08-30
申请人： Tony James Lough , Dieter H. Hermsmeier , Erika Varkonyi-Gasic , Justin Sweetman , Ilkka J. Havukkala , Helene Belanger , Richard L. S. Forster , Keith R. Hudson
发明人： Tony James Lough , Dieter H. Hermsmeier , Erika Varkonyi-Gasic , Justin Sweetman , Ilkka J. Havukkala , Helene Belanger , Richard L. S. Forster , Keith R. Hudson
IPC分类号： C12N15/29 , C12N15/11 , C12N15/82
CPC分类号： C07K14/415 , C12N15/827
摘要： The present application discloses plant polynucleotides, their encoded polypeptide sequences, and sRNA sequences which are putative regulators of long-distance florigenic signaling and flowering control. Methods of using these sequences related to long-distance florigenic signalling, including modifying the occurrence, timing and extent of flower development by modulating the florigenic signaling pathway, are also disclosed.
摘要翻译：本申请公开了植物多核苷酸，其编码的多肽序列和作为长距离发花信号和开花控制的假定调节子的sRNA序列。还公开了使用与长距离发花信号相关的这些序列的方法，包括通过调节发生发信号通路修饰花发育的发生，时间和程度。

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