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    • 2. 发明申请
    • Systemic small RNA signaling system in plants
    • 系统小RNA信号系统在植物中
    • US20050050588A1
    • 2005-03-03
    • US10871841
    • 2004-06-17
    • William LucasByung-Chun YooTony LoughErika Varkonyi-Gasic
    • William LucasByung-Chun YooTony LoughErika Varkonyi-Gasic
    • A01H1/00C07H21/04C07K14/415C12N5/04C12N9/22C12N15/82C12Q20060101
    • C07K14/415C12N15/8261Y02A40/146
    • Systemic translocation of RNA exerts non-cell-autonomous epigenetic control over plant development and defense. The present invention provides a polynucleotide sequence encoding a component of the protein machinery involved in small RNA trafficking, Cucurbita maxima PHLOEM SMALL RNA-BINDING PROTEIN1 (CmPSRB1), and the corresponding polypeptide sequence. Homologs of the disclosed sequences exist in other plant species. The invention also provides polynucleotide sequences of phloem small RNAs involved in signaling. Methods are also provided for using these PSRB1 and small RNA sequences and genetic constructs comprising these sequences to alter (e.g., prevent, reduce or elevate) non-cell autonomous signaling events in plants involving small RNA metabolism. These signaling events are involved in a broad spectrum of plant physiological and biochemical processes, including, for example, flowering, coordination of plant growth and development, systemic resistance to pathogens, responses to environmental stresses, e.g., heat, drought, salinity, systemic gene silencing (e.g., viral infections), biomass distribution, regulation of carbon metabolism and control of plant size and developmental timing and patterning.
    • RNA的系统易位对植物发育和防御发挥非细胞自主的表观遗传控制。 本发明提供了编码涉及小RNA运输的蛋白质机械的组分的多核苷酸序列,南瓜(Cucurbita maxima)PHLOEM SMALL RNA-BINDING PROTEIN1(CmPSRB1)以及相应的多肽序列。 所公开序列的同源物存在于其他植物物种中。 本发明还提供涉及信号传导的韧皮部小RNA的多核苷酸序列。 还提供了使用这些PSRB1和小RNA序列以及包含这些序列的遗传构建体来改变(例如,预防,减少或提高)涉及小RNA代谢的植物中的非细胞自主信号传导事件的方法。 这些信号事件涉及广泛的植物生理和生物化学过程,包括例如开花,植物生长和发育的协调,对病原体的系统抗性,对环境胁迫的反应,例如热,干旱,盐度,全身基因 沉默(例如病毒感染),生物量分布,碳代谢调节和植物大小控制以及发育时间和图案化。