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1. 发明授权

US5486462A Differentiative expression modules 失效
标题翻译：微分表达模块
公开(公告)号：US5486462A
公开(公告)日：1996-01-23
申请号：US900512
申请日：1992-06-17
申请人： William J. Rutter , Michael D. Wlaker , Thomas Edlund , Anne M. Boulet
发明人： William J. Rutter , Michael D. Wlaker , Thomas Edlund , Anne M. Boulet
IPC分类号： C07K14/62 , C12N9/64 , C12N15/85 , C12N5/06 , C12N15/11 , C12N15/17 , C12N15/67
CPC分类号： C12N9/6421 , C07K14/62 , C12N15/85 , C12N2830/008 , C12N2830/30 , C12N2830/85
摘要： More effectively controlled expression of DNA sequences in coding desired heterologous proteins is achieved in differentiated eucaryotic cells by methods of this invention. Disclosed herein are control modules derived from selectively expressed genes of eucaryotic cells, such as, for example, insulin and chymotrypsin genes. These control elements contain cis-acting sequences which are responsive to indigenous trans-acting substances in the differentiated cell, which substances control the expression of the gene. Such cis-acting elements occur within the promoter region of such selectively expressed genes, and also in the five prime flanking region of the coding sequence in a position upstream of the promoter. These upstream enhancer sequences may be located using the methods disclosed herein, and ligated into differentiative expression modules for production of desired heterologous proteins.
摘要翻译：通过本发明的方法在分化的真核细胞中实现编码所需异源蛋白质的DNA序列的更有效控制的表达。本文公开了衍生自真核细胞的选择性表达基因的控制模块，例如胰岛素和胰凝乳蛋白酶基因。这些控制元件含有对分化细胞中本地反式作用物质有反应的顺式作用序列，该物质控制该基因的表达。这种顺式作用元件发生在这些选择性表达的基因的启动子区域内，以及在启动子上游位置的编码序列的五个侧翼区域中。可以使用本文公开的方法定位这些上游增强子序列，并将其连接到用于产生所需异源蛋白质的差异表达模块中。

2. 发明授权

US5849989A Insulin promoter factor, and uses related thereto 失效
公开(公告)号：US5849989A
公开(公告)日：1998-12-15
申请号：US320148
申请日：1994-10-07
申请人： Thomas Edlund
发明人： Thomas Edlund
IPC分类号： C07K14/47 , C12N1/21 , C12N15/85 , C12N5/00 , C12N15/00
CPC分类号： C12N15/8509 , A01K67/0276 , C07K14/4702 , C07K14/4705 , A01K2217/05 , A01K2217/075 , A01K2227/105 , A01K2267/03 , A01K2267/0325 , A01K2267/0331 , C12N2800/30
摘要： The present invention relates to the discovery in eukaryotic cells, particularly mammalian cells, of novel a transcriptional regulatory factor, referred to hereinafter as "Insulin Promoter Factor 1" or "Ipf1".

3. 发明授权

US5472691A Superoxide dismutase 失效
标题翻译：超氧化物歧化酶
公开(公告)号：US5472691A
公开(公告)日：1995-12-05
申请号：US125744
申请日：1993-09-24
申请人： Stefan Marklund , Thomas Edlund
发明人： Stefan Marklund , Thomas Edlund
IPC分类号： A61K38/44 , C07K16/40 , C12N9/02 , C12N15/53
CPC分类号： A61K38/446 , C07K16/40 , C12N9/0089
摘要： A superoxide dismutase originally found in extracellular body fluids and therefore termed extracellular superoxide dismutase (EC-SOD) is prepared by growing a cell line, preferably of mammalian origin, producing EC-SOD and recovering the EC-SOD secreted from the cells or by inserting a DNA sequence encoding EC-SOD into a suitable vector, introducing the recombinant vector into a host cell, growing the cell and recovering the EC-SOD produced.EC-SOD may be used for the prophylaxis or treatment of diseases or disorders associated with the presence or formation of superoxide radicals.
摘要翻译：最初在细胞外体液中发现的超氧化物歧化酶，因此称为细胞外超氧化物歧化酶（EC-SOD）是通过生长优选哺乳动物来源的细胞系产生EC-SOD并回收从细胞分泌的EC-SOD或通过插入将编码EC-SOD的DNA序列转化成合适的载体，将重组载体导入宿主细胞，培养细胞并回收产生的EC-SOD。 EC-SOD可用于预防或治疗与超氧自由基的存在或形成相关的疾病或病症。

4. 发明授权

US5366729A Non-glycosylated variants of extracellular superoxide dismutase (EC-SOD) 失效
标题翻译：细胞外超氧化物歧化酶（EC-SOD）的非糖基化变体
公开(公告)号：US5366729A
公开(公告)日：1994-11-22
申请号：US856077
申请日：1992-05-13
申请人： Stefan Marklund , Thomas Edlund
发明人： Stefan Marklund , Thomas Edlund
IPC分类号： C07K16/40 , C12N9/02 , C12N15/53 , A61K37/50 , C12N5/10
CPC分类号： C12N9/0089 , C07K16/40
摘要： Extracellular superoxide dismutase (EC-SOD) variants having the superoxide dismutating property of the native EC-SOD and having a modified (reduced or increased) binding to heparin as compared to recombinant EC-SOD C as well as compositions comprising such variants. The EC-SOD variants are polypeptides comprising: 1) amino acids 1-193 of native EC-SOD C and 2) an amino acid sequence which is based on, but different from amino acid moieties 194-222 of recombinant EC-SOD C, either by being truncated or prolonged at the C-terminal end or by having substituted or otherwise modified one or more amino acid moieties of the sequence. Another EC-SOD variant is one which differs from recombinant EC-SOD C by being a glycosylation-free mutant. The variants may be produced by recombinant DNA techniques and are useful in the treatment of various diseases.
摘要翻译： PCT No.PCT / DK90 / 00238 Sec。 371日期：1992年5月13日 102（e）日期1992年5月13日PCT提交1990年9月17日PCT公布。公开号WO91 / 04315 1991年4月4日。与重组EC-SOD C相比，具有天然EC-SOD的超氧化物歧化性质并具有修饰（还原或增加的）肝素结合的细胞外超氧化物歧化酶（EC-SOD）变体以及包含这些变体的组合物。 EC-SOD变体是多肽，其包含：1）天然EC-SOD C的氨基酸1-193; 2）基于但不同于重组EC-SOD C的氨基酸部分194-222的氨基酸序列，通过在C-末端被截短或延长，或者通过取代或以其它方式修饰序列的一个或多个氨基酸部分。另一种EC-SOD变体是通过无糖基化突变体与重组EC-SOD C不同的变体。变异体可以通过重组DNA技术产生并且可用于治疗各种疾病。

5. 发明授权

US5248603A Superoxide dismutase 失效
标题翻译：超氧化物歧化酶
公开(公告)号：US5248603A
公开(公告)日：1993-09-28
申请号：US897624
申请日：1992-06-12
申请人： Stefan Marklund , Thomas Edlund
发明人： Stefan Marklund , Thomas Edlund
IPC分类号： A61K38/44 , C07K16/40 , C12N9/02 , C12N15/53
CPC分类号： A61K38/446 , C07K16/40 , C12N9/0089
摘要： A superoxide dismutase originally found in extracellular body fluids and therefore termed extracellular superoxide dismutase (EC-SOD) is prepared by growing a cell line, preferably of mammalian origin, producing EC-SOD and recovering the EC-SOD secreted from the cells or by inserting a DNA sequence encoding EC-SOD into a suitable vector, introducing the recombinant vector into a host cell, growing the cell and recovering the EC-SOD produced.EC-SOD may be used for the prophylaxis or treatment of diseases or disorders associated with the presence or formation of superoxide radicals.
摘要翻译：最初在细胞外体液中发现的超氧化物歧化酶，因此称为细胞外超氧化物歧化酶（EC-SOD）是通过生长优选哺乳动物来源的细胞系产生EC-SOD并回收从细胞分泌的EC-SOD或通过插入将编码EC-SOD的DNA序列转化成合适的载体，将重组载体导入宿主细胞，培养细胞并回收产生的EC-SOD。 EC-SOD可用于预防或治疗与超氧化物基团的存在或形成有关的疾病或病症。

6. 发明授权

US06908990B2 Insulin promoter factor, and uses related thereto 失效
标题翻译：胰岛素促进因子及其相关用途
公开(公告)号：US06908990B2
公开(公告)日：2005-06-21
申请号：US09759847
申请日：2001-01-12
申请人： Thomas Edlund
发明人： Thomas Edlund
IPC分类号： C07K14/47 , C12N1/21 , C12N15/85 , C07H21/04 , C07K17/00 , C12P19/34 , C12Q1/68 , G01N33/53
CPC分类号： C12N15/8509 , A01K67/0276 , A01K2217/05 , A01K2217/075 , A01K2227/105 , A01K2267/03 , A01K2267/0325 , A01K2267/0331 , C07K14/4702 , C07K14/4705 , C12N2800/30
摘要： The present invention relates to the discovery in eukaryotic cells, particularly mammalian cells, of novel a transcriptional regulatory factor, referred to hereinafter as “Insulin Promoter Factor 1” or “Ipf1”.
摘要翻译：本发明涉及在真核细胞特别是哺乳动物细胞中发现新的转录调节因子，以下称为“胰岛素启动子因子1”或“Ipf1”。

7. 发明授权

US06197945B1 Insulin promoter factor, and uses related thereto 失效
标题翻译：胰岛素促进因子及其相关用途
公开(公告)号：US06197945B1
公开(公告)日：2001-03-06
申请号：US09031898
申请日：1998-02-27
申请人： Thomas Edlund
发明人： Thomas Edlund
IPC分类号： C07H2102
CPC分类号： C12N15/8509 , A01K67/0276 , A01K2217/05 , A01K2217/075 , A01K2227/105 , A01K2267/03 , A01K2267/0325 , A01K2267/0331 , C07K14/4702 , C07K14/4705 , C12N2800/30
摘要： The present invention relates to the discovery in eukaryotic cells, particularly mammalian cells, of novel a transcriptional regulatory factor, referred to hereinafter as “Insulin Promoter Factor 1” or “Ipf1”.
摘要翻译：本发明涉及在真核细胞特别是哺乳动物细胞中发现新的转录调节因子，以下称为“胰岛素启动子因子1”或“Ipf1”。

8. 发明授权

US06566092B1 DNA encoding a vertebrate homolog of hedgehog, Vhh-1, expressed by the notochord, and uses thereof 失效
标题翻译：编码由脊索动物表达的刺猬，Vhh-1的脊椎动物同源物的DNA及其用途
公开(公告)号：US06566092B1
公开(公告)日：2003-05-20
申请号：US08700393
申请日：1997-02-27
申请人： Thomas M. Jessell , Jane Dodd , Henk Roelink , Thomas Edlund
发明人： Thomas M. Jessell , Jane Dodd , Henk Roelink , Thomas Edlund
IPC分类号： C12N1512
CPC分类号： C07K14/461 , A01K2217/05 , A61K38/00 , C07H21/04 , C07K14/43581 , C07K14/463 , C07K14/475 , C12N5/0619
摘要： This invention provides an isolated nucleic acid molecule encoding a vhh-1 protein, an isolated protein which is a vhh-1 protein, vectors comprising an isolated nucleic acid molecule encoding a vhh-1 protein, mamunalian cells comprising such vectors, antibodies directed to a vhh-1 protein, nucleic acid probes useful for detecting a nucleic acid molecule encoding a vhh-1 protein, pharmaceutical compositions related to the vhh-1 proteins, nonhuman transgenic animals which express a normal or a mutant vhh-1 protein. This invention further provides methods for inducing differentiation of floor plate cell, motor neuron, generating ventral neurons and treatments for alleviating abnormalities associated with the vhh-1 protein.
摘要翻译：本发明提供编码vhh-1蛋白质的分离的核酸分子，分离的蛋白质，其是vhh-1蛋白质，包含编码vhh-1蛋白质的分离的核酸分子的载体，包含该载体的哺乳动物细胞，针对 vhh-1蛋白，用于检测编码vhh-1蛋白的核酸分子的核酸探针，与vhh-1蛋白相关的药物组合物，表达正常或突变型vhh-1蛋白的非人转基因动物。本发明还提供了诱导地板细胞，运动神经元，产生腹侧神经元的分化和减轻与vhh-1蛋白相关的异常的治疗的方法。

9. 发明授权

US06235885B1 Rat hedgehog protein-1 (Vhh-1) 失效
标题翻译：大鼠刺猬蛋白-1（Vhh-1）
公开(公告)号：US06235885B1
公开(公告)日：2001-05-22
申请号：US08757230
申请日：1996-11-27
申请人： Thomas M. Jessell , Jane Dodd , Henk Roelink , Thomas Edlund
发明人： Thomas M. Jessell , Jane Dodd , Henk Roelink , Thomas Edlund
IPC分类号： C07K14475
CPC分类号： C07K14/461 , A01K2217/05 , A61K38/00 , C07H21/04 , C07K14/43581 , C07K14/463 , C07K14/475 , C12N5/0619
摘要： This invention provides an isolated nucleic acid molecule encoding a vertebrate Vhh-1 protein. The preferred embodiments are rat and human Vhh-1 protein. This invention provides purified vertebrate Vhh-1 protein and a method to purify same. This invention provides isolated nucleic acid molecules encoding a human and a rat Vhh-1 protein and the purified rat and human Vhh-1 protein encoded by same. This invention provides a vector encoding a vertebrate Vhh-1 protein and mammalian cells comprising such vectors. This invention provides antibodies directed to a vertebrate Vhh-1 protein. This invention further provides nucleic acid probes useful for detecting a nucleic acid molecule encoding a vertebrate Vhh-1 protein. This invention provides pharmaceutical compounds comprising the human Vhh-1 protein. This invention provides nonhuman transgenic animals which express DNA encoding a vertebrate Vhh-1 protein. This invention provides treatments for alleviating abnormalities associated with the vertebrate Vhh-1 protein.
摘要翻译：本发明提供了编码脊椎动物Vhh-1蛋白质的分离的核酸分子。优选的实施方案是大鼠和人Vhh-1蛋白。本发明提供了纯化的脊椎动物Vhh-1蛋白质及其纯化方法。本发明提供编码人和大鼠Vhh-1蛋白的分离的核酸分子和由其编码的纯化的大鼠和人Vhh-1蛋白。本发明提供编码脊椎动物Vhh-1蛋白的载体和包含这些载体的哺乳动物细胞。本发明提供了针对脊椎动物Vhh-1蛋白的抗体。本发明还提供了可用于检测编码脊椎动物Vhh-1蛋白的核酸分子的核酸探针。本发明提供包含人Vhh-1蛋白质的药物化合物。本发明提供了表达编码脊椎动物Vhh-1蛋白的DNA的非人类转基因动物。本发明提供了减轻与脊椎动物Vhh-1蛋白相关的异常的治疗方法。

10. 发明授权

US5788961A Superoxide dismutase 失效
公开(公告)号：US5788961A
公开(公告)日：1998-08-04
申请号：US446104
申请日：1995-05-19
申请人： Stefan Marklund , Thomas Edlund
发明人： Stefan Marklund , Thomas Edlund
IPC分类号： A61K38/44 , C07K16/40 , C12N9/02 , C12N15/53
CPC分类号： A61K38/446 , C07K16/40 , C12N9/0089
摘要： A superoxide dismutase originally found in extracellular body fluids and therefore termed extracellular superoxide dismutase (EC-SOD) is prepared by growing a cell line, preferably of mammalian origin, producing EC-SOD and recovering the EC-SOD secreted from the cells or by inserting a DNA sequence encoding EC-SOD into a suitable vector, introducing the recombinant vector into a host cell, growing the cell and recovering the EC-SOD produced. EC-SOD may be used for the prophylaxis or treatment of diseases or disorders associated with the presence or formation of superoxide radicals.

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