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    • 3. 发明授权
    • Epitope-captured antibody display
    • 表位捕获抗体显示
    • US07741062B2
    • 2010-06-22
    • US12420001
    • 2009-04-07
    • William J. DowerSteven E. Cwirla
    • William J. DowerSteven E. Cwirla
    • G01N33/53
    • G01N33/6842C07K16/28C07K16/2878C40B30/04G01N33/531G01N33/6845G01N33/6857G01N2035/00158G01N2600/00
    • Reagents and methods for detecting target proteins in a sample are provided. The reagents include a replicable genetic package, a protein displayed on an exterior surface of the package that is expressed from a heterologous nucleic acid borne by the package, and one or more antibodies complexed with the expressed protein and which have an open binding site for a target protein. Thus, a segment of the nucleic acid encodes for an epitope that is shared by the expressed polypeptide and the target protein. The reagents can be utilized individually or as part of a library or an array to bind target proteins within protein samples to form one or more complexes. By determining the sequence of the segment of the heterologous nucleic acid of a package within a complex, one can identify the target protein since the segment encodes for an epitope that is shared by the expressed and target proteins.
    • 提供了用于检测样品中靶蛋白的试剂和方法。 试剂包括可复制的遗传包装,显示在由包装所携带的异源核酸表达的包装外表面上的蛋白质,以及与表达的蛋白质复合的一种或多种抗体,并且具有用于 靶蛋白。 因此,一段核酸编码由表达的多肽和靶蛋白共享的表位。 试剂可以单独使用或作为文库或阵列的一部分来结合蛋白质样品中的靶蛋白以形成一种或多种复合物。 通过确定复合物内的包装的异源核酸的片段的序列,可以鉴定靶蛋白,因为片段编码由表达的和靶蛋白共享的表位。