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1. 发明授权

US5403710A Nucleic acid probes and methods for detecting pathogenic candida yeasts 失效
标题翻译：核酸探针和检测致病念珠菌酵母的方法
公开(公告)号：US5403710A
公开(公告)日：1995-04-04
申请号：US76283
申请日：1993-06-11
申请人： William G. Weisburg , Susan M. Barns , David J. Lane , Jeffrey F. Lemontt
发明人： William G. Weisburg , Susan M. Barns , David J. Lane , Jeffrey F. Lemontt
IPC分类号： G01N33/53 , C07H21/00 , C12N15/09 , C12Q1/04 , C12Q1/68 , C12R1/72 , C07H17/00 , C12N1/00 , C12P19/34
CPC分类号： C12Q1/6895 , Y10S435/921 , Y10S435/922 , Y10S435/923 , Y10S435/924
摘要： Nucleic acid probes are described for detecting yeasts capable of causing Candida infection, specifically yeasts of the genera Candida, Torulopsis, and Yarrowia. The preferred probes are complementary to ribonucleic acid sequences unique to specific Candida species, or groups of such species, and as such can detect the rRNA, rDNA, or polymerase chain reaction amplification products of these genes. Methods for detecting the etiological agent of human Candida fungemia utilizing these probes are also provided.
摘要翻译：描述了用于检测能够引起念珠菌感染的酵母的核酸探针，特别是假丝酵母属，Torulopsis属和耶氏酵母属的酵母菌。优选的探针与特异性假丝酵母属物种或这些物种的组特有的核糖核酸序列互补，因此可以检测这些基因的rRNA，rDNA或聚合酶链反应扩增产物。还提供了利用这些探针检测人类念珠菌真菌病原体病原体的方法。

2. 发明授权

US5817789A Chimeric proteins for use in transport of a selected substance into cells 失效
标题翻译：用于将选定物质运送到细胞中的嵌合蛋白
公开(公告)号：US5817789A
公开(公告)日：1998-10-06
申请号：US470058
申请日：1995-06-06
申请人： Michael W. Heartlein , Jeffrey F. Lemontt , Michael F. Concino
发明人： Michael W. Heartlein , Jeffrey F. Lemontt , Michael F. Concino
IPC分类号： C12N15/09 , A61K35/12 , A61K38/00 , A61K38/22 , A61K39/395 , A61P3/06 , A61P43/00 , C07K14/705 , C07K14/79 , C07K19/00 , C12N5/00 , C12N15/12 , C12N15/62 , C12N5/10
CPC分类号： C07K14/79 , C12N15/62 , A61K38/00 , C07K2319/00 , C07K2319/20 , C07K2319/74
摘要： Chimeric proteins, which bind a cell surface receptors, useful in transporting a selected substance present in extracellular fluids, such as blood or lymph, into cells; quantitative assays for the selected substance using chimeric proteins; DNA encoding the chimeric proteins; plasmids which contain DNA encoding the chimeric proteins; mammalian cells, modified to contain DNA encoding the chimeric proteins, which express and, optionally, secrete the chimeric proteins; a method of producing the chimeric proteins; a method of isolating the chimeric proteins; a method of using the chimeric proteins to assay the selected substance; and a method of reducing extracellular levels of the selected substance through administration of the chimeric protein, which results in transport of the selected substance into cells.
摘要翻译：嵌合蛋白，其结合细胞表面受体，其可用于将存在于细胞外液体（例如血液或淋巴）中的选定物质输送到细胞中; 使用嵌合蛋白的所选物质的定量测定; 编码嵌合蛋白的DNA; 含有编码嵌合蛋白质的DNA的质粒; 哺乳动物细胞，被修饰成含有编码嵌合蛋白的DNA，其表达和任选地分泌嵌合蛋白质; 制备嵌合蛋白质的方法; 分离嵌合蛋白的方法; 使用嵌合蛋白质来测定所选择的物质的方法; 以及通过施用嵌合蛋白降低所选物质的细胞外水平的方法，其导致所选择的物质转运到细胞中。

3. 发明授权

US5344773A Human uterine tissue plasminogen activator produced by recombinant DNA 失效
标题翻译：人重组DNA产生的人子宫组织纤溶酶原激活物
公开(公告)号：US5344773A
公开(公告)日：1994-09-06
申请号：US782686
申请日：1985-10-01
申请人： Cha-Mer Wei , Nancy Hsiung , Vermuri B. Reddy , Jeffrey F. Lemontt , William Dackowski , Richard Douglas , Edward S. Cole , Richard D. Purcell, Jr. , David Tai-Yui Lau
发明人： Cha-Mer Wei , Nancy Hsiung , Vermuri B. Reddy , Jeffrey F. Lemontt , William Dackowski , Richard Douglas , Edward S. Cole , Richard D. Purcell, Jr. , David Tai-Yui Lau
IPC分类号： C12N15/09 , A61K35/12 , A61K36/06 , A61K38/00 , A61K38/46 , C07K1/20 , C07K1/22 , C07K14/005 , C07K14/195 , C12N1/16 , C12N5/00 , C12N5/10 , C12N9/60 , C12N9/64 , C12N9/72 , C12N15/00 , C12N15/58 , C12N15/81 , C12N15/86 , C12P21/02 , C12R1/865 , C12R1/91 , A61K37/547
CPC分类号： C12N9/6459 , C12N15/81 , C12N15/85 , C12Y304/21069 , A61K38/00 , C12N2795/14143 , C12N2830/00 , C12N2830/002 , C12N2830/702 , C12N2830/85
摘要： A replicable expression vector capable, in a transformed host mammallian cell, of expressing a DNA sequence encoding active human TPA which, when expressed in the host cell, yields active TPA which lacks one or more of the TPA-bound N-linked carbohydrate moieties of naturally occurring human TPA.
摘要翻译：在转化的宿主哺乳动物细胞中能够表达编码活性人TPA的DNA序列的可复制的表达载体，其在宿主细胞中表达时产生缺乏一种或多种TPA结合的N-连接的碳水化合物部分的活性TPA 天然存在的人类TPA。

4. 发明授权

US06262026B1 Chimeric proteins for use in transport of a selected substance into cells 有权
标题翻译：用于将选定物质运送到细胞中的嵌合蛋白
公开(公告)号：US06262026B1
公开(公告)日：2001-07-17
申请号：US09285310
申请日：1999-04-02
申请人： Michael W. Heartlein , Jeffrey F. Lemontt , Michael F. Concino
发明人： Michael W. Heartlein , Jeffrey F. Lemontt , Michael F. Concino
IPC分类号： C07K14705
CPC分类号： C07K14/79 , A61K38/00 , C07K2319/00 , C07K2319/20 , C07K2319/74 , C12N15/62
摘要： Chimeric proteins which comprise a ligand-binding domain of a first receptor and a carrier domain which tends a cell surface receptor other than the first receptor, useful in transporting a selected substance present in extracellular fluids, such as blood or lymph, into cells; quantitative assays for the selected substance using chimeric proteins; DNA encoding the chimeric proteins; plasmids which contain DNA encoding the chimeric proteins; mammalian cells, modified to contain DNA encoding the chimeric proteins, which express and, optionally, secrete the chimeric proteins; a method of producing the chimeric proteins; a method of isolating the chimeric proteins; a method of using the chimeric proteins to assay the selected substance; and a method of reducing extracellular levels of the selected substance through administration of the chimeric protein, which results in transport of the selected substance into cells.
摘要翻译：包含第一受体的配体结合结构域和倾向于第一受体以外的细胞表面受体的载体结构域的嵌合蛋白质可用于将存在于细胞外液体（例如血液或淋巴）中的选定物质转运到细胞中; 使用嵌合蛋白的所选物质的定量测定; 编码嵌合蛋白的DNA; 含有编码嵌合蛋白质的DNA的质粒; 哺乳动物细胞，被修饰成含有编码嵌合蛋白的DNA，其表达和任选地分泌嵌合蛋白质; 制备嵌合蛋白质的方法; 分离嵌合蛋白的方法; 使用嵌合蛋白质来测定所选择的物质的方法; 以及通过施用嵌合蛋白降低所选物质的细胞外水平的方法，其导致所选择的物质转运到细胞中。

5. 发明授权

US06858578B2 Chimeric proteins for use in transport of a selected substance into cells 有权
标题翻译：用于将选定物质运送到细胞中的嵌合蛋白
公开(公告)号：US06858578B2
公开(公告)日：2005-02-22
申请号：US09753385
申请日：2001-01-03
申请人： Michael W. Heartlein , Jeffrey F. Lemontt , Michael F. Concino
发明人： Michael W. Heartlein , Jeffrey F. Lemontt , Michael F. Concino
IPC分类号： C12N15/09 , A61K35/12 , A61K38/00 , A61K38/22 , A61K39/395 , A61P3/06 , A61P43/00 , C07K14/705 , C07K14/79 , C07K19/00 , C12N5/00 , C12N15/12 , C12N15/62 , A61K38/16 , C07K14/47
CPC分类号： C07K14/79 , A61K38/00 , C07K2319/00 , C07K2319/20 , C07K2319/74 , C12N15/62
摘要： Chimeric proteins useful in transporting a selected substance present in extracellular fluids, such as blood or lymph, into cells; quantitative assays for the selected substance using chimeric proteins; DNA encoding the chimeric proteins; plasmids which contain DNA encoding the chimeric proteins; mammalian cells, modified to contain DNA encoding the chimeric proteins, which express and, optionally, secrete the chimeric proteins; a method of producing the chimeric proteins; a method of isolating the chimeric proteins; a method of using the chimeric proteins to assay the selected substance; and a method of reducing extracellular levels of the selected substance through administration of the chimeric protein, which results in transport of the selected substance into cells.
摘要翻译：用于将存在于细胞外液体（例如血液或淋巴）中的选定物质转运到细胞中的嵌合蛋白; 使用嵌合蛋白的所选物质的定量测定; 编码嵌合蛋白的DNA; 含有编码嵌合蛋白质的DNA的质粒; 哺乳动物细胞，被修饰成含有编码嵌合蛋白的DNA，其表达和任选地分泌嵌合蛋白质; 制备嵌合蛋白质的方法; 分离嵌合蛋白的方法; 使用嵌合蛋白质来测定所选择的物质的方法; 以及通过施用嵌合蛋白降低所选物质的细胞外水平的方法，其导致所选择的物质转运到细胞中。

6. 发明授权

US5658726A Detecting eukaryotic microorganisms 失效
标题翻译：检测真核微生物
公开(公告)号：US5658726A
公开(公告)日：1997-08-19
申请号：US860295
申请日：1992-03-27
申请人： Jeffrey F. Lemontt
发明人： Jeffrey F. Lemontt
IPC分类号： C12Q1/04 , C12Q1/68 , C12R1/725 , C12R1/865 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6895 , C12Q1/6888 , Y10S435/81 , Y10S435/921 , Y10S435/922 , Y10S435/924
摘要： A method for constructing a cDNA probe for use in detecting in a sample, under conditions of predetermined stringency, a target organism belonging to a strain of fungal microorganisms, and not detecting in the sample under such conditions a reference microorganism or any prokaryotic microorganism, includes the steps of determining a nucleotide base sequence in a variable region of small subunit ribosomal RNA from the target organism, the variable region being a region of the small subunit ribosomal RNA that is poorly conserved among eukaryotes and having no corresponding region in prokaryotes; comparing the nucleotide base sequence in the corresponding region of small subunit ribosomal RNA from the reference microorganism, and selecting as a useful probe site a subsequence within the determined sequence; and synthesizing a cDNA complementary to the useful probe site, the cDNA being the probe. Nucleic acid probes are constructed according to the disclosed method. A method for using such a probe for detecting a strain of fungal microorganisms in a sample includes the steps of providing such a probe, deriving RNA from the sample, contacting the probe with the RNA under conditions that allow the probe to hybridize with small subunit rRNA to form hybrid nucleic acid complexes, and detecting the hybrid nucleic acid complexes as an indication of the presence in the sample of the organism. A kit includes such a probe and instructions for its use.
摘要翻译：一种用于构建用于在预定严格条件下在样品中检测属于真菌微生物菌株的目标生物体并且在该条件下在该条件下检测参考微生物或任何原核生物微生物的cDNA探针的方法，包括确定来自目标生物体的小亚基核糖体RNA可变区核苷酸碱基序列的步骤，可变区是真核生物中不太保守且在原核生物中没有相应区域的小亚基核糖体RNA区域; 比较来自参照微生物的小亚基核糖体RNA相应区域中的核苷酸碱基序列，并选择确定序列内的子序列作为有用的探针位点; 并合成与有用探针位点互补的cDNA，cDNA为探针。根据所公开的方法构建核酸探针。使用这种探针来检测样品中真菌微生物菌株的方法包括以下步骤：提供这样的探针，从样品中导出RNA，使探针与RNA在允许探针与小亚基rRNA杂交的条件下接触RNA 以形成杂交核酸复合物，并且检测杂交核酸复合物作为生物样品中存在的指示。套件包括这样的探头和使用说明书。

7. 发明授权

US6027921A Chimeric proteins for use in transport of a selected substance into cells and DNA encoding chimeric proteins 失效
标题翻译：用于将选择的物质转运到细胞中的嵌合蛋白和编码嵌合蛋白的DNA
公开(公告)号：US6027921A
公开(公告)日：2000-02-22
申请号：US37188
申请日：1998-03-09
申请人： Michael W. Heartlein , Jeffrey F. Lemontt , Michael F. Concino
发明人： Michael W. Heartlein , Jeffrey F. Lemontt , Michael F. Concino
IPC分类号： C12N15/09 , A61K35/12 , A61K38/00 , A61K38/22 , A61K39/395 , A61P3/06 , A61P43/00 , C07K14/705 , C07K14/79 , C07K19/00 , C12N5/00 , C12N15/12 , C12N15/62 , C12N5/10 , C07K14/47 , C12N15/10
CPC分类号： C07K14/79 , C12N15/62 , A61K38/00 , C07K2319/00 , C07K2319/20 , C07K2319/74
摘要： Chimeric proteins, which comprise a ligand-binding domain of a first receptor and a carrier domain which binds a cell surface receptor other than the first receptor, useful in transporting a selected substance present in extracellular fluids, such as blood or lymph, into cells; quantitative assays for the selected substance using chimeric proteins; DNA encoding the chimeric proteins; plasmids which contain DNA encoding the chimeric proteins; mammalian cells, modified to contain DNA encoding the chimeric proteins, which express and, optionally, secrete the chimeric proteins; a method of producing the chimeric proteins; a method of isolating the chimeric proteins; a method of using the chimeric proteins to assay the selected substance; and a method of reducing extracellular levels of the selected substance through administration of the chimeric protein, which results in transport of the selected substance into cells.
摘要翻译：嵌合蛋白，其包含第一受体的配体结合结构域和结合不同于第一受体的细胞表面受体的载体结构域，其可用于将存在于细胞外液体（例如血液或淋巴）中的选定物质输送到细胞中; 使用嵌合蛋白的所选物质的定量测定; 编码嵌合蛋白的DNA; 含有编码嵌合蛋白质的DNA的质粒; 哺乳动物细胞，被修饰成含有编码嵌合蛋白的DNA，其表达和任选地分泌嵌合蛋白质; 制备嵌合蛋白质的方法; 分离嵌合蛋白的方法; 使用嵌合蛋白质来测定所选择的物质的方法; 以及通过施用嵌合蛋白降低所选物质的细胞外水平的方法，其导致所选择的物质转运到细胞中。

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