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1. 发明授权

US5976798A Methods for detecting mitochondrial mutations diagnostic for Alzheimer's disease and methods for determining heteroplasmy of mitochondrial nucleic acid 失效
标题翻译：检测线粒体突变诊断阿尔茨海默病的方法和线粒体核酸异质性检测方法
公开(公告)号：US5976798A
公开(公告)日：1999-11-02
申请号：US810599
申请日：1997-02-28
申请人： William Davis Parker , Corinna Herrnstadt , Soumitra Ghosh , Eoin D. Fahy
发明人： William Davis Parker , Corinna Herrnstadt , Soumitra Ghosh , Eoin D. Fahy
IPC分类号： C12N15/09 , C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6883 , C12Q1/6806 , C12Q2600/156 , C12Q2600/16
摘要： The present invention relates to methods of detecting genetic mutations in mitochondrial cytochrome oxidase c genes that segregate with Alzheimer's Disease and methods for determining the amount of heteroplasmy of mitochondrial nucleic acid. The invention provides methods for detecting such mutations, as a diagnostic for Alzheimer's Disease, either before or after the onset of clinical symptoms.
摘要翻译：本发明涉及检测与阿尔茨海默氏病分离的线粒体细胞色素氧化酶c基因中的遗传突变的方法和确定线粒体核酸异质性量的方法。本发明提供了在临床症状发作之前或之后检测这种突变作为阿尔茨海默病诊断的方法。

2. 发明授权

US6027883A Optimal procedure for isolation of mutant mitochondrial alleles 失效
标题翻译：分离突变体线粒体等位基因的最佳方法
公开(公告)号：US6027883A
公开(公告)日：2000-02-22
申请号：US757438
申请日：1996-11-27
申请人： Corinna Herrnstadt , Soumitra Ghosh , Eoin D. Fahy , Robert E. Davis
发明人： Corinna Herrnstadt , Soumitra Ghosh , Eoin D. Fahy , Robert E. Davis
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6806 , C12Q1/6858 , C12Q2600/156 , C12Q2600/16
摘要： A sample preparation procedure for mitochondrial (mt) DNA analysis is described. The present method for isolating mtDNA uses sedimentation techniques for separating erythrocytes from lymphocytes and platelets (crude buffy coat fraction), followed by DNA extraction from the crude buffy coat fraction by boiling in water. This approach improves the yield of mutant DNA, enhancing the sensitivity of subsequent mutation interrogation techniques and allowing for meaningful statistical treatment of the degree of heteroplasmy within the mitochondrial DNA.
摘要翻译：描述了线粒体（mt）DNA分析的样品制备程序。目前用于分离mtDNA的方法使用沉淀技术从淋巴细胞和血小板（粗血沉棕黄层级分离）中分离红细胞，然后通过在水中沸腾从粗血沉棕黄层级分提取DNA。这种方法提高了突变DNA的产量，提高了随后的突变询问技术的敏感性，并允许有意义的统计学处理线粒体DNA内的异质性程度。

3. 发明授权

US06489095B2 Diagnostic method based on quantification of extramitochondrial DNA 失效
公开(公告)号：US06489095B2
公开(公告)日：2002-12-03
申请号：US09098079
申请日：1998-06-15
申请人： Corinna Herrnstadt , Soumitra S. Ghosh , William Clevenger , Eoin D. Fahy , Robert E. Davis
发明人： Corinna Herrnstadt , Soumitra S. Ghosh , William Clevenger , Eoin D. Fahy , Robert E. Davis
IPC分类号： C12Q168
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q2600/156 , C12Q2600/172
摘要： Compositions and methods based on quantification of extramitochondrial DNA (exmtDNA) sequences are provided that are useful for detecting the presence of or risk for having a disease associated with altered mitochondrial function, and for identifying agents suitable for treating such diseases. The exmtDNA sequences have strong homology to authentic mitochondrial DNA (mtDNA) sequences.

4. 发明授权

US06441149B1 Diagnostic method based on quantification of extramitochondrial DNA 失效
标题翻译：基于线粒体DNA定量的诊断方法
公开(公告)号：US06441149B1
公开(公告)日：2002-08-27
申请号：US09302681
申请日：1999-04-30
申请人： Corinna Herrnstadt , Soumitra S. Ghosh , William Clevenger , Eoin D. Fahy , Robert E. Davis
发明人： Corinna Herrnstadt , Soumitra S. Ghosh , William Clevenger , Eoin D. Fahy , Robert E. Davis
IPC分类号： C07H2102
CPC分类号： C12Q1/6827 , C12Q1/6883 , C12Q2600/156 , C12Q2600/172
摘要： Compositions and methods based on quantification of extramitochondrial DNA (exmtDNA) sequences are provided that are useful for detecting the presence of or risk for having a disease associated with altered mitochondrial function, and for identifying agents suitable for treating such diseases. The exmtDNA sequences have strong homology to authentic mitochondrial DNA (mtDNA) sequences.
摘要翻译：提供了基于定量线粒体DNA（exmtDNA）序列的组合物和方法，其可用于检测与改变的线粒体功能相关的疾病的存在或风险，以及用于鉴定适合于治疗这些疾病的药剂。 exmtDNA序列与真实的线粒体DNA（mtDNA）序列具有很强的同源性。

5. 发明授权

US5663242A End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids 失效
标题翻译：将寡核苷酸末端连接到用于捕获和检测核酸的聚丙烯酰胺固体支持物上
公开(公告)号：US5663242A
公开(公告)日：1997-09-02
申请号：US414019
申请日：1995-03-31
申请人： Soumitra Shanker Ghosh , Eoin D. Fahy
发明人： Soumitra Shanker Ghosh , Eoin D. Fahy
IPC分类号： C07H21/00 , C12Q1/68 , C08F20/56 , C12P19/34 , C12Q1/70
CPC分类号： C07H21/00
摘要： Methods for covalent attachment of oligonucleotides to solid supports such that substantially all of the oligonucleotides are attached via their 5'-ends are provided. The solid supports with attached oligonucleotides are produced. Thiol-oligonucleotides are attached to bromoacetyl-derivatized polyacrylamide supports, or conversely, bromoacetyl-oligonucleotides are immobilized on thiol-polyacrylamide supports. In a further aspect, bromoacetyl-derivatized oligonucleotides, and polyacrylamide supports with linked oligonucleotides produced by coupling bromoacetyl-derivatized oligonucleotides with thiol-derivatized polyacrylamide solid supports or by coupling thiol-derivatized oligonucleotides with bromoacetyl-derivatized polyacrylamide supports as well as methods for capture of nucleic acids by oligonucleotides attached to polyacrylamide solid supports, either by direct capture or in sandwich hybridization formats are provided.
摘要翻译：提供了将寡核苷酸共价连接到固体支持物的方法，使得基本上所有的寡核苷酸通过其5'-末端连接。产生具有连接的寡核苷酸的固体支持物。硫醇寡核苷酸连接到溴乙酰基衍生的聚丙烯酰胺载体上，或者相反地，溴乙酰基寡核苷酸固定在硫醇 - 聚丙烯酰胺载体上。在另一方面，溴乙酰基衍生的寡核苷酸和具有通过将溴乙酰基衍生的寡核苷酸与硫醇衍生的聚丙烯酰胺固体支持物偶联或通过将硫醇衍生的寡核苷酸与溴乙酰基衍生的聚丙烯酰胺载体偶联产生的链接寡核苷酸的聚丙烯酰胺载体以及捕获提供了通过直接捕获或以夹心杂交形式连接到聚丙烯酰胺固体支持物上的寡核苷酸的核酸。

6. 发明授权

US5478893A End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids 失效
标题翻译：将寡核苷酸末端连接到用于捕获和检测核酸的聚丙烯酰胺固体支持物上
公开(公告)号：US5478893A
公开(公告)日：1995-12-26
申请号：US102474
申请日：1993-08-05
申请人： Soumitra S. Ghosh , Eoin D. Fahy
发明人： Soumitra S. Ghosh , Eoin D. Fahy
IPC分类号： C07H21/00 , C12Q1/68 , C08F120/56 , C07H21/02 , C07H21/04
CPC分类号： C07H21/00
摘要： Methods for covalent attachment of oligonucleotides to solid supports such that substantially all of the oligonucleotides are attached via their 5'-ends are provided. The solid supports with attached oligonucleotides are produced. Thiol-oligonucleotides are attached to bromoacetyl-derivatized polyacrylamide supports, or conversely, bromoacetyl-oligonucleotides are immobilized on thiol-polyacrylamide supports. In a further aspect, bromoacetyl-derivatized oligonucleotides, and polyacrylamide supports with linked oligonucleotides produced by coupling bromoacetyl-derivatized oligonucleotides with thiol-derivatized polyacrylamide solid supports or by coupling thiol-derivatized oligonucleotides with bromoacetyl-derivatized polyacrylamide supports as well as methods for capture of nucleic acids by oligonucleotides attached to polyacrylamide solid supports, either by direct capture or in sandwich hybridization formats are provided.
摘要翻译：提供了将寡核苷酸共价连接到固体支持物的方法，使得基本上所有的寡核苷酸通过其5'-末端连接。产生具有连接的寡核苷酸的固体支持物。硫醇寡核苷酸连接到溴乙酰基衍生的聚丙烯酰胺载体上，或者相反地，溴乙酰基寡核苷酸固定在硫醇 - 聚丙烯酰胺载体上。在另一方面，溴乙酰基衍生的寡核苷酸和具有通过将溴乙酰基衍生的寡核苷酸与硫醇衍生的聚丙烯酰胺固体支持物偶联或通过将硫醇衍生的寡核苷酸与溴乙酰基衍生的聚丙烯酰胺载体偶联产生的链接寡核苷酸的聚丙烯酰胺载体以及捕获提供了通过直接捕获或以夹心杂交形式连接到聚丙烯酰胺固体支持物上的寡核苷酸的核酸。

7. 发明授权

US5237016A End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids 失效
标题翻译：将寡核苷酸结合到聚丙烯酰胺固体支持物上用于捕获和检测核酸
公开(公告)号：US5237016A
公开(公告)日：1993-08-17
申请号：US293893
申请日：1989-01-05
申请人： Soumitra S. Ghosh , Eoin D. Fahy
发明人： Soumitra S. Ghosh , Eoin D. Fahy
IPC分类号： C07H21/00 , C12Q1/68
CPC分类号： C07H21/00
摘要： This invention concerns methods and means for covalent attachment of oligonucleotides to solid supports substantially at their 5'-ends. According to the invention thiol-oligonucleotides are attached to bromoacetyl-derivatized polyacrylamide supports, or conversely, bromoacetyl-oligonucleotides are immobilized on thiol-polyacrylamide supports.In a further aspect, this invention relates to bromoacetyl-oligonucleotides that may be immobilized on thiol-polyacrylamide solid supports, thiol-oligonucleotides immobilized on bromoacetyl-derivatized polyacrylamide supports as well as to methods for capture of nucleic acids by oligonucleotides attached to polyacrylamide solid supports, either by direct capture or in sandwich hybridization formats.
摘要翻译：本发明涉及将寡核苷酸共价连接到基本上在其5'端的固体支持物的方法和手段。根据本发明，硫醇 - 寡核苷酸连接到溴乙酰基衍生的聚丙烯酰胺载体上，或者相反地，溴乙酰基寡核苷酸被固定在巯基 - 聚丙烯酰胺载体上。在另一方面，本发明涉及可固定在硫醇 - 聚丙烯酰胺固体支持物上的溴乙酰基寡核苷酸，固定在溴乙酰基衍生的聚丙烯酰胺载体上的硫醇寡核苷酸以及通过与聚丙烯酰胺固体支持物连接的寡核苷酸捕获核酸的方法，通过直接捕获或夹心杂交形式。

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