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1. 发明授权

US06489146B2 End-complementary polymerase reaction 失效
标题翻译：末端互补聚合酶反应
公开(公告)号：US06489146B2
公开(公告)日：2002-12-03
申请号：US09245802
申请日：1999-02-05
申请人： Willem P.C. Stemmer , Robert J. Lipshutz
发明人： Willem P.C. Stemmer , Robert J. Lipshutz
IPC分类号： C12P1934
CPC分类号： C12N15/10 , C12N15/64 , C12N15/66 , C12Q1/6853 , C12Q1/686 , C12Q2537/143 , C12Q2531/125 , C12Q2525/307 , C12Q2525/151 , C12Q2525/155
摘要： The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof and for assembling large polynucleotides from component polynucleotides, each involving generating concatemers formed by PCR amplification of overlapping fragments.
摘要翻译：本发明涉及用于扩增和检测核酸或其混合物中包含的任何靶核酸序列并用于从组分多核苷酸组装大多核苷酸的方法，每个涉及产生通过重叠片段的PCR扩增形成的连接体。

2. 发明申请

US20120252681A1 EVOLUTION OF WHOLE CELLS AND ORGANISMS BY RECURSIVE SEQUENCE RECOMBINATION 有权
标题翻译：通过重新序列重组实现全细胞和有机体的发育
公开(公告)号：US20120252681A1
公开(公告)日：2012-10-04
申请号：US13310332
申请日：2011-12-02
申请人： Stephen del Cardayre , Matthew Tobin , Willem P.C. Stemmer , Jon E. Ness , Jeremy Minshull , Phillip Patten , Venkiteswaran Subramanian , Linda A. Castle , Claus M. Krebber , Steven H. Bass , Ying-Xin Zhang , Tony Cox , Gjalt Huisman , Ling Yuan , Joseph A. Affholter
发明人： Stephen del Cardayre , Matthew Tobin , Willem P.C. Stemmer , Jon E. Ness , Jeremy Minshull , Phillip Patten , Venkiteswaran Subramanian , Linda A. Castle , Claus M. Krebber , Steven H. Bass , Ying-Xin Zhang , Tony Cox , Gjalt Huisman , Ling Yuan , Joseph A. Affholter
IPC分类号： C40B10/00
CPC分类号： C12N15/102 , C07K14/245 , C12N15/1027 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12P17/162 , C12Q1/68 , C12Q1/6811 , C12Q1/6827 , C12R1/465
摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
摘要翻译：本发明提供了采用迭代循环重组和选择/筛选全细胞和生物体以获得所需性质的方法的方法。这些性质的实例包括增加的重组原性，基因组拷贝数以及蛋白质和次级代谢物的表达和/或分泌能力。

3. 发明申请

US20100144547A1 METHODS AND COMPOSITIONS FOR CELLULAR AND METABOLIC ENGINEERING 失效
标题翻译：细胞和代谢工程的方法和组合
公开(公告)号：US20100144547A1
公开(公告)日：2010-06-10
申请号：US12557812
申请日：2009-09-11
申请人： Jeremy Minshull , Willem P.C. Stemmer
发明人： Jeremy Minshull , Willem P.C. Stemmer
IPC分类号： C40B30/06
CPC分类号： C12N15/1027 , C12N15/1058
摘要： The present invention is generally directed to the evolution of new metabolic pathways and the enhancement of bioprocessing through a process herein termed recursive sequence recombination. Recursive sequence recombination entails performing iterative cycles of recombination and screening or selection to “evolve” individual genes, whole plasmids or viruses, multigene clusters, or even whole genomes. Such techniques do not require the extensive analysis and computation required by conventional methods for metabolic engineering.
摘要翻译：本发明一般涉及新代谢途径的演变以及通过本文中称为递归序列重组的方法增强生物处理。递归序列重组需要执行重组和筛选或选择的“进化”个体基因，整个质粒或病毒，多基因簇或甚至全基因组的迭代循环。这些技术不需要传统的代谢工程方法所需的广泛的分析和计算。

4. 发明申请

US20090149331A1 Methods and compositions for polypeptide engineering 有权
公开(公告)号：US20090149331A1
公开(公告)日：2009-06-11
申请号：US12069011
申请日：2008-02-05
申请人： Phillip A. Patten , Willem P.C. Stemmer
发明人： Phillip A. Patten , Willem P.C. Stemmer
IPC分类号： C40B10/00
CPC分类号： C12N15/1031 , A61K38/45 , A61K48/00 , C07K14/43595 , C07K14/545 , C07K14/56 , C07K16/00 , C07K2317/565 , C07K2317/622 , C12N7/00 , C12N9/0069 , C12N9/1007 , C12N9/16 , C12N9/86 , C12N15/10 , C12N15/1027 , C12N15/1034 , C12N15/1037 , C12N15/1058 , C12N15/52 , C12N15/64 , C12N15/67 , C12N2795/14043 , C12N2795/14143 , C12Q1/6811 , C12Q1/6853 , C12Q1/686 , C40B40/02 , C40B40/08 , C40B50/06 , G01N33/68 , G01N33/6845
摘要： Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.

5. 发明申请

US20080318795A1 Methods for making character strings, polynucleotides and polypeptides having desired characteristics 有权
标题翻译：制备具有所需特征的字符串，多核苷酸和多肽的方法
公开(公告)号：US20080318795A1
公开(公告)日：2008-12-25
申请号：US11982405
申请日：2007-10-31
申请人： Sergey A. Selifonov , Willem P.C. Stemmer , Claes Gustafsson , Matthew Tobin , Stephen del Cardayre , Phillip A. Patten , Jeremy Minshull , Lorraine J. Giver
发明人： Sergey A. Selifonov , Willem P.C. Stemmer , Claes Gustafsson , Matthew Tobin , Stephen del Cardayre , Phillip A. Patten , Jeremy Minshull , Lorraine J. Giver
IPC分类号： C40B10/00
CPC分类号： G06F19/16 , B01J2219/007 , C40B50/02 , G06F19/14 , G06F19/18 , G06F19/22 , G06F19/24
摘要： In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided.
摘要翻译：在硅胶核酸重组方法中，提供了利用遗传算子和通过计算机改组方法制备的文库的相关综合系统。

6. 发明申请

US20080227649A1 Methods and compositions for cellular and metabolic engineering 有权
标题翻译：细胞和代谢工程的方法和组成
公开(公告)号：US20080227649A1
公开(公告)日：2008-09-18
申请号：US12011767
申请日：2008-01-28
申请人： Jeremy Minshull , Willem P.C. Stemmer
发明人： Jeremy Minshull , Willem P.C. Stemmer
IPC分类号： C40B10/00
CPC分类号： C12N15/1027 , C12N15/1058
摘要： The present invention is generally directed to the evolution of new metabolic pathways and the enhancement of bioprocessing through a process herein termed recursive sequence recombination. Recursive sequence recombination entails performing iterative cycles of recombination and screening or selection to “evolve” individual genes, whole plasmids or viruses, multigene clusters, or even whole genomes. Such techniques do not require the extensive analysis and computation required by conventional methods for metabolic engineering.
摘要翻译：本发明一般涉及新代谢途径的演变以及通过本文中称为递归序列重组的方法增强生物处理。递归序列重组需要执行重组和筛选或选择的“进化”个体基因，整个质粒或病毒，多基因簇或甚至全基因组的迭代循环。这些技术不需要传统的代谢工程方法所需的广泛的分析和计算。

7. 发明申请

US20100331193A1 Methods for generating polynucleotides having desired characteristics by iterative selection and recombination 审中-公开
标题翻译：通过迭代选择和重组产生具有所需特征的多核苷酸的方法
公开(公告)号：US20100331193A1
公开(公告)日：2010-12-30
申请号：US12557938
申请日：2009-09-11
申请人： Willem P.C. Stemmer , Andreas Crameri
发明人： Willem P.C. Stemmer , Andreas Crameri
IPC分类号： C40B20/00
CPC分类号： C12N15/1058 , C07K14/43595 , C07K14/545 , C07K16/00 , C07K2317/565 , C07K2317/622 , C12N9/2471 , C12N9/86 , C12N15/1027 , C12N15/1037 , C12N15/52 , C12N15/64 , C12Q1/68 , C12Q1/6811 , C12Y302/01023 , C40B40/02 , Y10S435/876
摘要： Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
摘要翻译：提供了用于工业和医药兴趣蛋白质进化的方法，包括进行重组和选择的方法。还公开了通过这些方法制备的组合物。

8. 发明申请

US20100184627A1 OLIGONUCLEOTIDE MEDIATED NUCLEIC ACID RECOMBINATION 有权
标题翻译：寡核苷酸介导的核酸重组
公开(公告)号：US20100184627A1
公开(公告)日：2010-07-22
申请号：US12557829
申请日：2009-09-11
申请人： Andreas Crameri , Willem P.C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , John E. Ness , Claes Gustafsson , Phillip A. Patten
发明人： Andreas Crameri , Willem P.C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , John E. Ness , Claes Gustafsson , Phillip A. Patten
IPC分类号： C40B50/00
CPC分类号： C12P21/06
摘要： Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
摘要翻译：提供重组核酸的方法，包括同源核酸。还提供了基因改组寡核苷酸家族及其在重组过程中的应用，以及聚合酶和连接酶介导的重组方法。

9. 发明申请

US20100056385A1 METHODS FOR IDENTIFYING SETS OF OLIGONUCLEOTIDES FOR USE IN AN IN VITRO RECOMBINATION PROCEDURE 有权
标题翻译：用于鉴定体外重组程序中使用寡核苷酸的方法
公开(公告)号：US20100056385A1
公开(公告)日：2010-03-04
申请号：US12557463
申请日：2009-09-10
申请人： Sergey A. Selifonov , Willem P.C. Stemmer , Claes Gustafsson , Matthew Tobin , Stephen del Cardayre , Phillip A. Patten , Jeremy Minshull , Lorraine J. Giver
发明人： Sergey A. Selifonov , Willem P.C. Stemmer , Claes Gustafsson , Matthew Tobin , Stephen del Cardayre , Phillip A. Patten , Jeremy Minshull , Lorraine J. Giver
IPC分类号： C40B30/02
CPC分类号： G06F19/16 , B01J2219/007 , C40B50/02 , G06F19/14 , G06F19/18 , G06F19/22 , G06F19/24
摘要： In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided.
摘要翻译：在硅胶核酸重组方法中，提供了利用遗传算子和通过计算机改组方法制备的文库的相关综合系统。

10. 发明申请

US20080287314A1 Methods for modulating cellular and organismal phenotypes 审中-公开
标题翻译：调节细胞和有机体表型的方法
公开(公告)号：US20080287314A1
公开(公告)日：2008-11-20
申请号：US12151739
申请日：2008-05-07
申请人： Willem P.C. Stemmer , Jeremy Minshull , Robert J. Keenan
发明人： Willem P.C. Stemmer , Jeremy Minshull , Robert J. Keenan
IPC分类号： C40B30/06
CPC分类号： C12N15/1079 , C07K2319/00 , C12N15/1027 , C12N15/1034 , C12N15/113 , C12N15/66 , C12N2310/111
摘要： Methods for identifying and controlling the genetic and metabolic pathways underlying complex phenotypes are provided. Conjoint polynucleotide segments that contribute to or disrupt elements of a multigenic phenotype are produced and expressed in cells of interest. Conjoint polynucleotide segments are recombined and/or mutated to give rise to libraries of recombinant concatamers which are expressed in cells of interest. Libraries of conjoint polynucleotide segments and recombinant concatamers are expressed episomally or integrated into the DNA of organelles or chromosomes. Cells are screened or selected to identify members of the population of cells exhibiting a desired phenotype. Libraries and vectors comprising conjoint polynucleotide segments and recombinant concatamers, as well as cells expressing such libraries and vectors or their components are provided. Kits containing conjoint polynucleotide segments, recombinant concatamers, vectors including such polynucleotides, and cells including such polynucleotides and vectors are provided.
摘要翻译：提供了鉴定和控制复杂表型下遗传和代谢途径的方法。有助于或破坏多基因表型的元件的连接多核苷酸片段在感兴趣的细胞中产生并表达。连接的多核苷酸片段被重组和/或突变以产生在感兴趣的细胞中表达的重组连接体的文库。联合多核苷酸片段和重组连接体的文库以附加方式表达或整合到细胞器或染色体的DNA中。筛选或选择细胞以鉴定呈现所需表型的细胞群体的成员。提供了包含组合多核苷酸片段和重组连接体的文库和载体，以及表达这些文库和载体或其组分的细胞。提供了含有联合多核苷酸片段，重组连接体，包含此类多核苷酸的载体和包含此类多核苷酸和载体的细胞的试剂盒。

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