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1. 发明授权

US08168447B2 Multiple component nanoparticles for multiplexed signaling and optical encoding 有权
标题翻译：用于复用信令和光编码的多组分纳米颗粒
公开(公告)号：US08168447B2
公开(公告)日：2012-05-01
申请号：US12066308
申请日：2006-10-10
申请人： Weihong Tan , Lin Wang , Chaoyong Yang
发明人： Weihong Tan , Lin Wang , Chaoyong Yang
IPC分类号： G01N33/552
CPC分类号： G01N33/582 , G01N33/5432 , G01N33/587 , Y10T428/2982
摘要： The present invention provides multiple-luminophore silica nanoparticles for multiplexed signaling in bioanalysis. In specific embodiments, two inorganic luminophores, Tris(2,2′-bipyridyl)osmium(II) bis(hexafluorophosphate) (OsBpy) and Tris(2,2′-bipyridyl)dichlororuthenium(II) hexahydrate (RuBpy), or three organic luminophores 5-Fluorescein isothiocyanate (5-FITC), 5-carboxyrhodamine 6G, succinimidyl ester (5-CR6G, SE), 6-carboxy-X-rhodamine, succinimidyl ester (6-ROX, SE) can be simultaneously entrapped inside silica nanoparticles at controlled ratios, with desirable sizes and required surface functionality. Single-wavelength excitation with multiple emission endows the nanoparticles with optical encoding capability for rapid and high-throughput multiplexed detection.
摘要翻译：本发明提供用于生物分析中多重信号传导的多发光二氧化硅纳米颗粒。在具体实施方案中，两种无机发光体，三（2,2'-联吡啶）锇（II）双（六氟磷酸盐）（OsBpy）和三（2,2'-联吡啶）六水合二钌（II）（RuBpy）或三种有机发光体5-氟代异硫氰酸酯（5-FITC），5-羧基罗丹胺6G，琥珀酰亚胺酯（5-CR6G，SE），6-羧基-X-罗丹明，琥珀酰亚胺酯（6-ROX，SE）可以同时包埋在二氧化硅纳米颗粒内具有所需的尺寸和所需的表面功能。具有多次发射的单波长激发使纳米颗粒具有光学编码能力，用于快速和高通量多路复用检测。

2. 发明申请

US20090017476A1 Multiple Component Nanoparticles for Multiplexed Signaling and Optical Encoding 有权
标题翻译：用于复用信令和光编码的多组分纳米颗粒
公开(公告)号：US20090017476A1
公开(公告)日：2009-01-15
申请号：US12066308
申请日：2006-10-10
申请人： Weihong Tan , Lin Wang , Chaoyong Yang
发明人： Weihong Tan , Lin Wang , Chaoyong Yang
IPC分类号： G01N33/53 , B32B1/00
CPC分类号： G01N33/582 , G01N33/5432 , G01N33/587 , Y10T428/2982
摘要： The present invention provides multiple-luminophore silica nanoparticles for multiplexed signaling in bioanalysis. In specific embodiments, two inorganic luminophores, Tris(2,2′-bipyridyl)osmium(II) bis(hexafluorophosphate) (OsBpy) and Tris(2,2′-bipyridyl)dichlororuthenium(II) hexahydrate (RuBpy), or three organic luminophores 5-Fluorescein isothiocyanate (5-FITC), 5-carboxyrhodamine 6G, succinimidyl ester (5-CR6G, SE), 6-carboxy-X-rhodamine, succinimidyl ester (6-ROX, SE) can be simultaneously entrapped inside silica nanoparticles at controlled ratios, with desirable sizes and required surface functionality. Single-wavelength excitation with multiple emission endows the nanoparticles with optical encoding capability for rapid and high-throughput multiplexed detection.
摘要翻译：本发明提供用于生物分析中多重信号传导的多发光二氧化硅纳米颗粒。在具体实施方案中，两种无机发光体，三（2,2'-联吡啶）锇（II）双（六氟磷酸盐）（OsBpy）和三（2,2'-联吡啶）六水合二钌（II）（RuBpy）或三种有机发光体5-氟代异硫氰酸酯（5-FITC），5-羧基罗丹胺6G，琥珀酰亚胺酯（5-CR6G，SE），6-羧基-X-罗丹明，琥珀酰亚胺酯（6-ROX，SE）可以同时包埋在二氧化硅纳米颗粒内具有所需的尺寸和所需的表面功能。具有多次发射的单波长激发使纳米颗粒具有光学编码能力，用于快速和高通量多路复用检测。

3. 发明申请

US20090156416A1 Hybrid Molecular Probe 审中-公开
标题翻译：杂交分子探针
公开(公告)号：US20090156416A1
公开(公告)日：2009-06-18
申请号：US11994965
申请日：2006-10-11
申请人： Weihong Tan , Chaoyong Yang
发明人： Weihong Tan , Chaoyong Yang
IPC分类号： C40B30/04 , C07H21/00 , C07K14/00 , G01N33/48
CPC分类号： C12Q1/6876 , Y10T436/143333
摘要： A system and method for analyzing a substance, in particular RNA in vivo, comprising a hybrid molecular probe, said probe comprising two single-stranded nucleic acid sequences tethered together with a polyethylene glycol polymer and fluorophores attached to either end of the sequences. When a probe of the invention hybridizes to a target substance (such as a target RKA sequence), fluorescence resonance energy transfer occurs between the two fluorophores to generate a visible signal.
摘要翻译：用于分析物质，特别是体内RNA的系统和方法，其包含杂交分子探针，所述探针包含与聚乙二醇聚合物连接的两条单链核酸序列和连接于序列任一端的荧光团。当本发明的探针与目标物质（例如目标RKA序列）杂交时，在两个荧光团之间发生荧光共振能量转移以产生可见信号。

4. 发明申请

US20100248385A1 MULTI-ACCEPTOR MOLECULAR PROBES AND APPLICATIONS THEREOF 失效
标题翻译：多受体分子探针及其应用
公开(公告)号：US20100248385A1
公开(公告)日：2010-09-30
申请号：US11570491
申请日：2005-06-17
申请人： Weihong Tan , Chaoyong Yang
发明人： Weihong Tan , Chaoyong Yang
IPC分类号： G01N21/76 , C07H21/00
CPC分类号： G01N33/542 , G01N33/5308 , Y10T436/143333
摘要： An oligonucleotide-based molecular probe includes at least one pin loop, the pin loop including a loop sequence complementary to a target sequence. A first stem sequence is attached to one end of the pin loop, the first stem having at least one fluorescent label attached thereto. A second stem sequence is attached to the other end of the pin loop. The second stem has a plurality of quencher molecules attached thereto.
摘要翻译：基于寡核苷酸的分子探针包括至少一个引脚环，引脚环包括与靶序列互补的环序列。第一茎序列连接到针环的一端，第一茎具有至少一个附着于其上的荧光标记。第二个茎序列连接到针环的另一端。第二茎具有多个与其连接的猝灭剂分子。

5. 发明授权

US08652851B2 Multi-acceptor molecular probes and applications thereof 失效
标题翻译：多受体分子探针及其应用
公开(公告)号：US08652851B2
公开(公告)日：2014-02-18
申请号：US11570491
申请日：2005-06-17
申请人： Weihong Tan , Chaoyong Yang
发明人： Weihong Tan , Chaoyong Yang
IPC分类号： G01N21/76
CPC分类号： G01N33/542 , G01N33/5308 , Y10T436/143333
摘要： An oligonucleotide-based molecular probe includes at least one pin loop, the pin loop including a loop sequence complementary to a target sequence. A first stem sequence is attached to one end of the pin loop, the first stem having at least one fluorescent label attached thereto. A second stem sequence is attached to the other end of the pin loop. The second stem has a plurality of quencher molecules attached thereto.
摘要翻译：基于寡核苷酸的分子探针包括至少一个引脚环，引脚环包括与靶序列互补的环序列。第一茎序列连接到针环的一端，第一茎具有至少一个附着于其上的荧光标记。第二个茎序列连接到针环的另一端。第二茎具有多个与其连接的猝灭剂分子。

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