专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08126649B2 Methods for generating a distribution of optimal solutions to nondeterministic polynomial optimization problems 有权
标题翻译：用于生成非确定性多项式优化问题的最优解的分布的方法
公开(公告)号：US08126649B2
公开(公告)日：2012-02-28
申请号：US12181056
申请日：2008-07-28
申请人： Wayne Frasch , David Spetzler , Justin York , Fusheng Xiong
发明人： Wayne Frasch , David Spetzler , Justin York , Fusheng Xiong
IPC分类号： G01N33/48 , C12Q1/68 , G06G7/58
CPC分类号： G06F19/24 , G06F19/22
摘要： The present invention provides methods for generating a distribution of optimal answers to a nondeterministic polynomial optimization problem by providing a plurality of solutions comprising input polynucleotides, wherein each solution comprises identical input polynucleotides; and wherein the number of solutions comprising polynucleotides equals a number of data inputs in the problem to be answered, and wherein each input polynucleotide comprises an x segment and a y segment; providing a plurality of solutions comprising connection polynucleotides wherein each solution comprises identical connection polynucleotides; and wherein the number of solutions comprising connection polynucleotides equals a number of unique connections that can be made between the different data inputs, and wherein each polynucleotide in the set of connection polynucleotides is complementary to the x segment of one input polynucleotide and to the y segment of one different input polynucleotide; combining the solutions comprising the input polynucleotides with the solutions comprising the connection polynucleotides to form a hybridization mixture, wherein the combining is done under conditions to promote formation of hybridization complexes between complementary polynucleotides, and wherein each individual connection polynucleotide is added at a concentration based on a weighted value assigned to the individual connection polynucleotide; ligating the polynucleotides that are present in the hybridization complexes to form ligation products; and determining a concentration of the ligation products, wherein the ligation products present at the highest concentration represent optimal answers to the nondeterministic polynomial optimization problem.
摘要翻译：本发明提供了通过提供包括输入多核苷酸的多种解决方案来产生对非确定性多项式优化问题的最优答案的分布的方法，其中每个溶液包含相同的输入多核苷酸; 并且其中包含多核苷酸的溶液的数量等于要回答的问题中的多个数据输入，并且其中每个输入多核苷酸包括x区段和y区段; 提供包含连接多核苷酸的多种溶液，其中每种溶液包含相同的连接多核苷酸; 并且其中包含连接多核苷酸的溶液的数量等于可以在不同数据输入之间进行的多个唯一连接，并且其中连接多核苷酸组中的每个多核苷酸与一个输入多核苷酸的x片段和y片段互补的一种不同的输入多核苷酸; 将包含输入多核苷酸的溶液与包含连接多核苷酸的溶液组合以形成杂交混合物，其中组合在促进互补多核苷酸之间形成杂交复合物的条件下进行，并且其中每个单独的连接多核苷酸以基于分配给个体连接多核苷酸的加权值; 连接存在于杂交复合物中的多核苷酸以形成连接产物; 并确定连接产物的浓度，其中以最高浓度存在的连接产物表示非确定性多项式优化问题的最佳答案。

2. 发明申请

US20090047677A1 METHODS FOR GENERATING A DISTRIBUTION OF OPTIMAL SOLUTIONS TO NONDETERMINISTIC POLYNOMIAL OPTIMIZATION PROBLEMS 有权
标题翻译：用于生成优化解决方案到非定常多项优化问题的方法
公开(公告)号：US20090047677A1
公开(公告)日：2009-02-19
申请号：US12181056
申请日：2008-07-28
申请人： Wayne Frasch , David Spetzler , Justin York , Fusheng Xiong
发明人： Wayne Frasch , David Spetzler , Justin York , Fusheng Xiong
IPC分类号： C12Q1/68
CPC分类号： G06F19/24 , G06F19/22
摘要： The present invention overcomes problems in prior art DNA-based computing methods for solving non-deterministic polynomial optimization problems, by providing methods that derive the most probable answers in a statistically significant manner that makes the methods scalable with increases in the number of data inputs, and thus makes the methods practical.
摘要翻译：本发明克服了现有技术的基于DNA的计算方法中用于解决非确定性多项式优化问题的问题，通过提供以统计有意义的方式导出最可能的答案的方法，使方法随着数据输入数量的增加而可扩展，从而使方法切实可行。

3. 发明申请

US20120196771A1 HIGH SPEED, HIGH FIDELITY, HIGH SENSITIVITY NUCLEIC ACID DETECTION 失效
标题翻译：高速，高灵敏度，高灵敏度的核酸检测
公开(公告)号：US20120196771A1
公开(公告)日：2012-08-02
申请号：US13299126
申请日：2011-11-17
申请人： Wayne Frasch , David Spetzler , Justin York
发明人： Wayne Frasch , David Spetzler , Justin York
IPC分类号： C40B40/00 , C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/6816 , C12Q1/6832 , C12Q2561/125 , C12Q2521/325
摘要： Methods, compositions, and kits for nucleic acid detection.
摘要翻译：用于核酸检测的方法，组合物和试剂盒。

4. 发明授权

US08084206B2 High speed, high fidelity, high sensitivity nucleic acid detection 有权
标题翻译：高速，高保真，高灵敏度的核酸检测
公开(公告)号：US08084206B2
公开(公告)日：2011-12-27
申请号：US12439061
申请日：2007-08-29
申请人： Wayne Frasch , David Spetzler , Justin York
发明人： Wayne Frasch , David Spetzler , Justin York
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6837 , C12Q1/6816 , C12Q1/6832 , C12Q2561/125 , C12Q2521/325
摘要： The present invention provides methods, compositions, and kits for nucleic acid detection.
摘要翻译：本发明提供用于核酸检测的方法，组合物和试剂盒。

5. 发明申请

US20100015616A1 High Speed, High Fidelity, High Sensitivity Nucleic Acid Detection 有权
标题翻译：高速，高保真，高灵敏度的核酸检测
公开(公告)号：US20100015616A1
公开(公告)日：2010-01-21
申请号：US12439061
申请日：2007-08-29
申请人： Wayne Frasch , David Spetzler , Justin York
发明人： Wayne Frasch , David Spetzler , Justin York
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/6816 , C12Q1/6832 , C12Q2561/125 , C12Q2521/325
摘要： The present invention provides methods, compositions, and kits for nucleic acid detection.
摘要翻译：本发明提供用于核酸检测的方法，组合物和试剂盒。

6. 发明申请

US20220195521A1 PROBES AND METHODS FOR MEASURING TANDEM REPEATS 有权
公开(公告)号：US20220195521A1
公开(公告)日：2022-06-23
申请号：US17498643
申请日：2021-10-11
申请人： Wayne Frasch , Fusheng Xiong
发明人： Wayne Frasch , Fusheng Xiong
IPC分类号： C12Q1/6876 , C12Q1/6827 , C12Q1/6851 , C12Q1/686
摘要： The present disclosure relates to nucleic acid probes and kits for determining the length of a region of tandem repeats in a subject's genome and methods of using the DNA probes for determining the length of a region of tandem repeats in a subject's genome. In some embodiments, the region of tandem repeats in telomeres.

7. 发明申请

US20090035751A1 Single Molecule Detection Using Molecular Motors 有权
标题翻译：使用分子电机的单分子检测
公开(公告)号：US20090035751A1
公开(公告)日：2009-02-05
申请号：US10582820
申请日：2004-12-17
申请人： Lyian He , Wayne Frasch
发明人： Lyian He , Wayne Frasch
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , C12Q1/6834 , C12Q2565/619 , C12Q2531/137 , C12Q2533/107 , C12Q2565/601 , C12Q2565/629 , C12Q2563/125
摘要： The present invention provides methods and compositions for highly sensitive nucleic acid detection, down to the single nucleic acid molecule level. In one aspect, the present invention provides methods for detecting a target nucleic acid comprising: (a) providing first and second target-specific nucleic acids, wherein the first and second target-specific nucleic acids each comprise sequences complementary to the target nucleic acid; wherein the first target specific nucleic acid is bound to a first affinity tag and the second target-specific nucleic acid is bound to a second affinity tag, wherein the first affinity tag is capable of binding to a molecular motor, and wherein the second affinity tag is capable of binding to a detection probe; (b) contacting the first and second target-specific nucleic acids to a sample under conditions whereby the first and second target-specific nucleic acids will hybridize to the target nucleic acid if the target nucleic acid is present in the sample, wherein upon hybridization to the target nucleic acid the first and second target-specific nucleic acids are directly adjacent to each other; (c) ligating the first and second target-specific nucleic acids together; (d) binding the molecular motor t˜ the first affinity tag and the detection probe to the second affinity tag; (e) inducing movement of the molecular motor; and (f) detecting movement of the molecular motor through the detection probe, wherein the movement of the molecular motor serves to detect the target nucleic acid in the sample.
摘要翻译：本发明提供用于高度敏感的核酸检测的方法和组合物，直到单个核酸分子水平。一方面，本发明提供了用于检测靶核酸的方法，包括：（a）提供第一和第二靶特异性核酸，其中第一和第二靶特异性核酸各自包含与靶核酸互补的序列; 其中所述第一靶特异性核酸与第一亲和标签结合，并且所述第二靶特异性核酸与第二亲和标签结合，其中所述第一亲和标签能够结合分子马达，并且其中所述第二亲和标签能够结合检测探针; （b）在条件下使第一和第二靶特异性核酸与样品接触，其中如果靶核酸存在于样品中，则第一和第二靶特异性核酸将与靶核酸杂交，其中在与靶核酸第一和第二靶特异性核酸彼此直接相邻; （c）将第一和第二靶特异性核酸连接在一起; （d）将第一亲和标签和检测探针的分子马达结合到第二亲和标签上; （e）诱导分子马达的运动; 和（f）检测分子马达通过检测探针的运动，其中分子马达的移动用于检测样品中的靶核酸。

8. 发明申请

US20060110738A1 Polarization-enhanced detector with gold nanorods for detecting nanoscale rotational motion and method therefor 有权
标题翻译：具有用于检测纳米级旋转运动的金纳米棒的极化增强检测器及其方法
公开(公告)号：US20060110738A1
公开(公告)日：2006-05-25
申请号：US10538534
申请日：2003-12-11
申请人： Wayne Frasch , Lars Chapsky
发明人： Wayne Frasch , Lars Chapsky
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： B82Y5/00 , B22F2998/00 , B82Y15/00 , B82Y20/00 , B82Y30/00 , C12Q1/6816 , G01P3/68 , G02B21/10 , C12Q2565/628 , C12Q2563/155 , C12Q2561/119 , B22F1/0025
摘要： A nanoscale motion detector attaches a gold nanorod (30) to the rotating arm (26) of a molecular structure (10) to cause the nanoparticle to rotate. The molecular structure is an F1-ATPase enzyme. The gold nanorod is exposed to a light source. The long axis of the gold nanorod scatters red light when the nanorod is in a first position. The short axis of the gold nanorod scatters green light when the nanorod is in a second position. A polarizing filter filters the red and green light to detect the rotational motion by observing alternating red and green lights. A detection DNA stand (50) is coupled between the gold nanorod and the molecular structure. The detection DNA strand hybridizes with a target DNA strand (58) if the target DNA strand matches the detection DNA strand to form a structural link between the molecular structure and gold nanorod.
摘要翻译：纳米尺度运动检测器将金纳米棒（30）附接到分子结构（10）的旋转臂（26），以使纳米颗粒旋转。分子结构是F1-ATP酶。金纳米棒暴露于光源。当纳米棒位于第一位置时，金纳米棒的长轴散射红光。当纳米棒处于第二位置时，金纳米棒的短轴分散绿色光。偏振滤光片通过观察交替的红色和绿色光来过滤红色和绿色光以检测旋转运动。检测DNA架（50）耦合在金纳米棒和分子结构之间。如果目标DNA链匹配检测DNA链，检测DNA链与靶DNA链（58）杂交，以形成分子结构和金纳米棒之间的结构连接。

9. 发明授权

US08076079B2 Single molecule detection using molecular motors 有权
标题翻译：使用分子马达进行单分子检测
公开(公告)号：US08076079B2
公开(公告)日：2011-12-13
申请号：US10582820
申请日：2004-12-17
申请人： Wayne Frasch , Lyian He
发明人： Wayne Frasch , Lyian He
IPC分类号： C12Q1/68 , C12M1/34 , C07H21/04 , C07H21/02
CPC分类号： C12Q1/6825 , C12Q1/6834 , C12Q2565/619 , C12Q2531/137 , C12Q2533/107 , C12Q2565/601 , C12Q2565/629 , C12Q2563/125
摘要： The present invention provides methods and compositions for highly sensitive nucleic acid detection, down to the single nucleic acid molecule level. In one aspect, the present invention provides methods for detecting a target nucleic acid comprising: (a) providing first and second target-specific nucleic acids, wherein the first and second target-specific nucleic acids each comprise sequences complementary to the target nucleic acid; wherein the first target specific nucleic acid is bound to a first affinity tag and the second target-specific nucleic acid is bound to a second affinity tag, wherein the first affinity tag is capable of binding to a molecular motor, and wherein the second affinity tag is capable of binding to a detection probe; (b) contacting the first and second target-specific nucleic acids to a sample under conditions whereby the first and second target-specific nucleic acids will hybridize to the target nucleic acid if the target nucleic acid is present in the sample, wherein upon hybridization to the target nucleic acid the first and second target-specific nucleic acids are directly adjacent to each other; (c) ligating the first and second target-specific nucleic acids together; (d) binding the molecular motor t˜ the first affinity tag and the detection probe to the second affinity tag; (e) inducing movement of the molecular motor; and (f) detecting movement of the molecular motor through the detection probe, wherein the movement of the molecular motor serves to detect the target nucleic acid in the sample.
摘要翻译：本发明提供用于高度敏感的核酸检测的方法和组合物，直到单个核酸分子水平。一方面，本发明提供了用于检测靶核酸的方法，包括：（a）提供第一和第二靶特异性核酸，其中第一和第二靶特异性核酸各自包含与靶核酸互补的序列; 其中所述第一靶特异性核酸与第一亲和标签结合，并且所述第二靶特异性核酸与第二亲和标签结合，其中所述第一亲和标签能够结合分子马达，并且其中所述第二亲和标签能够结合检测探针; （b）在条件下使第一和第二靶特异性核酸与样品接触，其中如果靶核酸存在于样品中，则第一和第二靶特异性核酸将与靶核酸杂交，其中在与靶核酸第一和第二靶特异性核酸彼此直接相邻; （c）将第一和第二靶特异性核酸连接在一起; （d）将分子马达t〜第一亲和标签和检测探针结合到第二亲和标签上; （e）诱导分子马达的运动; 和（f）检测分子马达通过检测探针的运动，其中分子马达的移动用于检测样品中的靶核酸。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式