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    • 10. 发明授权
    • High throughput prion assays
    • 高通量朊病毒试验
    • US07741054B2
    • 2010-06-22
    • US11273487
    • 2005-11-14
    • Walter EberleWerner StockBaerbel Winter
    • Walter EberleWerner StockBaerbel Winter
    • G01N33/48C12Q1/37
    • G01N33/6896C12Q1/6869G01N33/54393G01N2400/00G01N2800/2828C12Q2565/501C12Q2533/101C12Q2527/137
    • The invention is directed to a method for performing a high throughput test to determine the presence of PrPsc in a tissue sample taken post mortem from brain, the test comprising the workflow of (A) sample preparation, (B) sample treatment, (C) sample analysis, (D) controls, and (E) classifying the results of the analysis as positive or negative. The invention is further directed to preparing a treated biological sample suspected of containing PrPsc such that it is suitable for specific detection of PrPsc, if present, the method comprising the steps of (a) homogenizing the sample; followed by (b) digesting the sample proteolytically by adding to the homogenate Proteinase K and incubating the sample; followed by (c) adding a peptidic protease inhibitor to the incubated mixture of step (b), thereby obtaining a treated biological sample suitable for specific detection of PrPsc, if present. The invention further provides a conditioned microwell plate. In addition, the invention provides a soluble fusion protein comprising one or more epitopes of prion protein as well as a carrier polypeptide.
    • 本发明涉及一种用于进行高通量测试以确定来自脑的死后采集的组织样品中PrPsc的存在的方法,该测试包括(A)样品制备,(B)样品处理,(C) 样本分析,(D)对照,(E)将分析结果分为正或负。 本发明进一步涉及制备怀疑含有PrPsc的经处理的生物样品,使其适合于PrPsc的特异性检测(如果存在),该方法包括以下步骤:(a)使样品均质化; 然后(b)通过加入匀浆蛋白酶K蛋白水解样品并孵育样品; 然后(c)向步骤(b)的培养的混合物中加入肽蛋白酶抑制剂,从而获得适合于特异性检测PrPsc(如果存在)的经处理的生物样品。 本发明进一步提供一种经调理的微孔板。 此外,本发明提供了包含一种或多种朊蛋白表位以及载体多肽的可溶性融合蛋白。