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1. 发明申请

US20100137551A1 Methods and materials for the production of D-lactic acid in yeast 审中-公开
标题翻译：在酵母中生产D-乳酸的方法和材料
公开(公告)号：US20100137551A1
公开(公告)日：2010-06-03
申请号：US12352133
申请日：2009-01-12
申请人： Vineet Rajgarhia , Catherine Asleson Dundon , Stacey Olson , Pirkko Suominen , Ben Hause
发明人： Vineet Rajgarhia , Catherine Asleson Dundon , Stacey Olson , Pirkko Suominen , Ben Hause
IPC分类号： C12P7/56 , C07D319/12 , C08G63/08
CPC分类号： C12P7/56 , C12N1/16 , C12N9/0006 , C12N15/815 , C12P7/625 , C12Q1/02 , G01N2333/39 , Y02P20/52
摘要： The present invention relates to biocatalysts that are cells, optimally of the Crabtree-negative phenotype, comprising expression vectors encoding genes heterologous to the cell that enable increased production of organic products. More specifically, the invention relates to genetically modified Kluyveromyces cells, methods for making the Kluyveromyces cells, and their use in production of organic products, particularly D-lactic acid.
摘要翻译：本发明涉及作为Crabtree阴性表型的细胞的生物催化剂，其包含编码与细胞异源的基因的表达载体，其能够增加有机产物的产生。更具体地，本发明涉及遗传修饰的克鲁维酵母细胞，制备克鲁维链酵母细胞的方法，以及它们在生产有机产物，特别是D-乳酸中的用途。

2. 发明申请

US20090253189A1 Lactic Acid-Producing Yeast Cells Having Nonfunctional L- or D-Lactate:Ferricytochrome C Oxidoreductase Cells 有权
标题翻译：具有非功能性L-或D-乳酸的乳酸生产酵母细胞：铁染色质C氧化还原酶细胞
公开(公告)号：US20090253189A1
公开(公告)日：2009-10-08
申请号：US12093757
申请日：2006-11-17
申请人： Matthew Miller , Pirkko Suominen , Aristos Aristidou , Benjamin Matthew Hause , Pim Van Hoek , Catherine Asleson Dundon
发明人： Matthew Miller , Pirkko Suominen , Aristos Aristidou , Benjamin Matthew Hause , Pim Van Hoek , Catherine Asleson Dundon
IPC分类号： C12P7/56 , C12N1/19 , C12N15/74
CPC分类号： C12N9/0006 , C12P7/56 , C12Y101/02003
摘要： Yeast cells having an exogenous lactate dehydrogenase gene ae modified by reducing L- or D-lactate:ferricytochrome c oxidoreductase activity in the cell. This leads to reduced consumption of lactate by the cell and can increase overall lactate yields in a fermentation process. Cells having the reduced I, or D-lactate:ferricytochrome c oxidoreductase activity can be screened for by resistance to organic acids such as lactic or glycolic acid.
摘要翻译：酵母细胞具有通过减少细胞中的L-或D-乳酸：铁染色体氧化还原酶活性而修饰的外源性乳酸脱氢酶基因。这导致细胞对乳酸盐的消耗降低，并且可以增加发酵过程中的总体乳酸盐产量。具有降低的I或D-乳酸：铁染色质c氧化还原酶活性的细胞可通过耐有机酸如乳酸或乙醇酸进行筛选。

3. 发明申请

US20090053782A1 YEAST CELLS HAVING DISRUPTED PATHWAY FROM DIHYDROXYACETONE PHOSPHATE TO GLYCEROL 审中-公开
标题翻译：具有从二羟基乙酸酯磷酸盐到甘油的破坏途径的YEAST细胞
公开(公告)号：US20090053782A1
公开(公告)日：2009-02-26
申请号：US12281286
申请日：2007-03-13
申请人： Catherine Asleson Dundon , Pirkko Suominen , Aristos Aristidou , Brian J. Rush , Kari Koivuranta , Benjamin Matthew Hause , Thomas William McMullin , Kevin Roberg-Perez
发明人： Catherine Asleson Dundon , Pirkko Suominen , Aristos Aristidou , Brian J. Rush , Kari Koivuranta , Benjamin Matthew Hause , Thomas William McMullin , Kevin Roberg-Perez
IPC分类号： C12P7/56 , C12N1/19
CPC分类号： B65G15/12 , B65G17/12 , C12N15/52 , C12P7/06 , C12P7/40 , C12P7/56 , E01B29/32 , Y02E50/17 , Y10T137/85986
摘要： Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the genera Kluyueromyces, Candida or Issatchenkia. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.
摘要翻译：酵母细胞被遗传修饰以破坏从二羟基丙酮到甘油的天然代谢途径。在某些方面，酵母细胞是克鲁维酵母属，假丝酵母属或发chen类。在其它方面，酵母细胞能够产生至少一种有机酸，如乳酸盐。酵母细胞比野生型菌株产生明显较少的甘油，并且通常产生更高产量的所需发酵产物。本发明的酵母细胞在培养时经常生长良好，尽管它们的甘油产量减少。

4. 发明授权

US08137953B2 Lactic acid-producing yeast cells having nonfunctional L- or D-lactate:ferricytochrome C oxidoreductase cells 有权
标题翻译：具有非功能性L-或D-乳酸的产酸酵母细胞：铁染色质C氧化还原酶细胞
公开(公告)号：US08137953B2
公开(公告)日：2012-03-20
申请号：US12093757
申请日：2006-11-17
申请人： Matthew Miller , Pirkko Suominen , Aristos Aristidou , Benjamin Matthew Hause , Pim Van Hoek , Catherine Asleson Dundon
发明人： Matthew Miller , Pirkko Suominen , Aristos Aristidou , Benjamin Matthew Hause , Pim Van Hoek , Catherine Asleson Dundon
IPC分类号： C12N1/19 , C12N1/00 , C12N15/70 , C12Q1/68
CPC分类号： C12N9/0006 , C12P7/56 , C12Y101/02003
摘要： Yeast cells having an exogenous lactate dehydrogenase gene ae modified by reducing L- or D-lactate:ferricytochrome c oxidoreductase activity in the cell. This leads to reduced consumption of lactate by the cell and can increase overall lactate yields in a fermentation process. Cells having the reduced L- or D-lactate:ferricytochrome c oxidoreductase activity can be screened for by resistance to organic acids such as lactic or glycolic acid.
摘要翻译：酵母细胞具有通过减少细胞中的L-或D-乳酸：铁染色体氧化还原酶活性而修饰的外源性乳酸脱氢酶基因。这导致细胞对乳酸盐的消耗降低，并且可以增加发酵过程中的总体乳酸盐产量。具有降低的L-乳酸或D-乳酸：细胞色素C氧化还原酶活性的细胞可通过耐有机酸如乳酸或乙醇酸进行筛选。

5. 发明授权

US09249420B2 Isobutanol production using yeasts with modified transporter expression 有权
标题翻译：使用具有改良转运蛋白表达的酵母的异丁醇生产
公开(公告)号：US09249420B2
公开(公告)日：2016-02-02
申请号：US13701247
申请日：2011-05-31
申请人： Catherine Asleson Dundon , Christopher Smith , Piruz Nahreini , Johan Thevelein , Sofie Saerens
发明人： Catherine Asleson Dundon , Christopher Smith , Piruz Nahreini , Johan Thevelein , Sofie Saerens
IPC分类号： C12P7/16 , C12N15/81 , C12N1/00 , C07K14/395
CPC分类号： C12P7/16 , C07K14/395 , C12N1/00 , C12N1/16 , C12N15/81 , Y02E50/10
摘要： The present invention relates to recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise one or more modifications resulting in the reduction in the expression and/or activity of an endogenous transporter protein. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译：本发明涉及包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。在本发明的各个方面，重组微生物可包含导致内源转运蛋白的表达和/或活性降低的一种或多种修饰。在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物，和非发酵酵母微生物。

6. 发明申请

US20120288910A1 METHODS OF INCREASING DIHYDROXY ACID DEHYDRATASE ACTIVITY TO IMPROVE PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS 审中-公开
标题翻译：增加二羟酸脱水酶活性的方法，以提高燃料，化学品和氨基酸的生产
公开(公告)号：US20120288910A1
公开(公告)日：2012-11-15
申请号：US13528106
申请日：2012-06-20
申请人： Jun Urano , Catherine Asleson Dundon , Peter Meinhold , Reid M. Renny Feldman , Aristos Aristidou , Andrew Hawkins , Thomas Buelter , Matthew Peters , Doug Lies , Stephanie Porter-Scheinman , Christopher Smith , Lynne H. Albert
发明人： Jun Urano , Catherine Asleson Dundon , Peter Meinhold , Reid M. Renny Feldman , Aristos Aristidou , Andrew Hawkins , Thomas Buelter , Matthew Peters , Doug Lies , Stephanie Porter-Scheinman , Christopher Smith , Lynne H. Albert
IPC分类号： C12P7/16 , C12N1/19
CPC分类号： C12P7/16 , C07K14/395 , C12N9/88 , C12P13/02 , C12P13/06 , C12P13/08 , C12Y402/01009 , Y02E50/10
摘要： The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译：本发明涉及包含一种或多种二羟基酸脱水酶（DHAD） - 生物合成途径的重组微生物，以及使用所述重组微生物产生源自所述DHAD需要生物合成途径的有益代谢物的方法。在本发明的各个方面，重组微生物可被工程化以过表达一种或多种编码一种或多种Aft蛋白或其同系物的多核苷酸。在一些实施方案中，重组微生物可以包含细胞溶质定位的DHAD酶。在另外的实施方案中，重组微生物可以包含线粒体定位的DHAD酶。在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物，和非发酵酵母微生物。

7. 发明申请

US20120028322A1 METHODS OF INCREASING DIHYDROXY ACID DEHYDRATASE ACTIVITY TO IMPROVE PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS 有权
标题翻译：增加二羟酸脱水酶活性的方法，以提高燃料，化学品和氨基酸的生产
公开(公告)号：US20120028322A1
公开(公告)日：2012-02-02
申请号：US13246693
申请日：2011-09-27
申请人： Catherine Asleson Dundon , Aristos Aristidou , Andrew Hawkins , Doug Lies , Lynne H. Albert
发明人： Catherine Asleson Dundon , Aristos Aristidou , Andrew Hawkins , Doug Lies , Lynne H. Albert
IPC分类号： C12N1/19 , C12P7/16
CPC分类号： C12P7/16 , C07K14/395 , C12N9/88 , C12P13/02 , C12P13/06 , C12P13/08 , C12Y402/01009 , Y02E50/10
摘要： The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces Glade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译：本发明涉及包含一种或多种二羟基酸脱水酶（DHAD） - 生物合成途径的重组微生物，以及使用所述重组微生物产生源自所述DHAD需要生物合成途径的有益代谢物的方法。在本发明的各个方面，重组微生物可被工程化以过表达一种或多种编码一种或多种Aft蛋白或其同系物的多核苷酸。在一些实施方案中，重组微生物可以包含细胞溶质定位的DHAD酶。在另外的实施方案中，重组微生物可以包含线粒体定位的DHAD酶。在本文所述的各种实施方案中，重组微生物可以是葡萄糖酵母的微生物，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，前WGD（全基因组复制）酵母微生物，和非发酵酵母微生物。

8. 发明授权

US08071358B1 Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids 有权
标题翻译：增加二羟基酸脱水酶活性以改善燃料，化学品和氨基酸生产的方法
公开(公告)号：US08071358B1
公开(公告)日：2011-12-06
申请号：US13228342
申请日：2011-09-08
申请人： Catherine Asleson Dundon , Aristos Aristidou , Andrew Hawkins , Doug Lies , Lynne Albert
发明人： Catherine Asleson Dundon , Aristos Aristidou , Andrew Hawkins , Doug Lies , Lynne Albert
IPC分类号： C12N1/00 , C07H21/02
CPC分类号： C12P7/16 , C07K14/395 , C12N9/88 , C12P13/02 , C12P13/06 , C12P13/08 , C12Y402/01009 , Y02E50/10
摘要： The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces Glade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译：本发明涉及包含一种或多种二羟基酸脱水酶（DHAD） - 生物合成途径的重组微生物，以及使用所述重组微生物产生源自所述DHAD需要生物合成途径的有益代谢物的方法。在本发明的各个方面，重组微生物可被工程化以过表达一种或多种编码一种或多种Aft蛋白或其同系物的多核苷酸。在一些实施方案中，重组微生物可以包含细胞溶质定位的DHAD酶。在另外的实施方案中，重组微生物可以包含线粒体定位的DHAD酶。在本文所述的各种实施方案中，重组微生物可以是葡萄糖酵母的微生物，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，前WGD（全基因组复制）酵母微生物，和非发酵酵母微生物。

9. 发明申请

US20110236942A1 Reduced by-product accumulation for improved production of isobutanol 无效
公开(公告)号：US20110236942A1
公开(公告)日：2011-09-29
申请号：US13076284
申请日：2011-03-30
申请人： Andrew Hawkins , Stephanie Porter-Scheinman , Catherine Asleson Dundon , Aristos Aristidou , Doug Lies , Julie Kelly
发明人： Andrew Hawkins , Stephanie Porter-Scheinman , Catherine Asleson Dundon , Aristos Aristidou , Doug Lies , Julie Kelly
IPC分类号： C12P7/16 , C12N1/19
CPC分类号： C12N15/81 , C12N9/0006 , C12P7/16 , C12Y101/01 , C12Y102/01005 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

10. 发明授权

US08017376B2 Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids 有权
标题翻译：增加二羟基酸脱水酶活性以改善燃料，化学品和氨基酸生产的方法
公开(公告)号：US08017376B2
公开(公告)日：2011-09-13
申请号：US12953884
申请日：2010-11-24
申请人： Catherine Asleson Dundon , Aristos Aristidou , Andrew Hawkins , Doug Lies , Lynne H. Albert
发明人： Catherine Asleson Dundon , Aristos Aristidou , Andrew Hawkins , Doug Lies , Lynne H. Albert
IPC分类号： C12N1/00 , C07H21/02
CPC分类号： C12P7/16 , C07K14/395 , C12N9/88 , C12P13/02 , C12P13/06 , C12P13/08 , C12Y402/01009 , Y02E50/10
摘要： The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译：本发明涉及包含一种或多种二羟基酸脱水酶（DHAD） - 生物合成途径的重组微生物，以及使用所述重组微生物产生源自所述DHAD需要生物合成途径的有益代谢物的方法。在本发明的各个方面，重组微生物可被工程化以过表达一种或多种编码一种或多种Aft蛋白或其同系物的多核苷酸。在一些实施方案中，重组微生物可以包含细胞溶质定位的DHAD酶。在另外的实施方案中，重组微生物可以包含线粒体定位的DHAD酶。在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物，和非发酵酵母微生物。

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