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    • 3. 发明授权
    • System and method for microwave cell lysing of small samples
    • 小样品微波裂解系统及方法
    • US06623945B1
    • 2003-09-23
    • US09397691
    • 1999-09-16
    • Vijay K. NairHerbert Goronkin
    • Vijay K. NairHerbert Goronkin
    • C12N1300
    • H05B6/806C12N1/066C12N13/00
    • Efficient cell lysis in small samples, i.e., samples less than one milliliter, is achieved by exposing the sample to microwave radiation in the frequency range of 18 to 26 GHz. The sample containing cells is supported in a wave-guide cavity, and a microwave source provides microwave radiation to the input port of the wave-guide cavity. A computer controls the frequency and source power level of the microwave radiation produced by the microwave source. The computer also monitors the input power level of the microwave radiation at the input port by means of an input power measuring instrument, the output power level at the output port by means of an output power measuring instrument, and the temperature of the sample by means of a thermocouple. In this way, the computer can control the operating parameters to achieve efficient cell lysis.
    • 通过将样品暴露于18至26GHz的频率范围内的微波辐射,可以实现小样品(即样品小于1毫升)的高效细胞裂解。 含有电池的样品被支撑在波导腔中,并且微波源向波导腔的输入端口提供微波辐射。 计算机控制由微波源产生的微波辐射的频率和源功率电平。 计算机还通过输入功率测量仪器监视输入端口处的微波辐射的输入功率电平,通过输出功率测量仪器输出端口处的输出功率电平,以及样品的温度 的热电偶。 以这种方式,计算机可以控制操作参数以实现有效的细胞裂解。