专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06746860B1 Paramyxovirus vectors used for transfer of foreign genes 失效
标题翻译：用于转移外来基因的副粘病毒载体
公开(公告)号：US06746860B1
公开(公告)日：2004-06-08
申请号：US09702498
申请日：2000-10-31
申请人： Tsuyoshi Tokusumi , Akihiro Iida , Mamoru Hasegawa , Yoshiyuki Nagai
发明人： Tsuyoshi Tokusumi , Akihiro Iida , Mamoru Hasegawa , Yoshiyuki Nagai
IPC分类号： C12N701
CPC分类号： C12N15/86 , C12N2760/18843 , C12N2830/42
摘要： A paramyxovirus vector capable of Extransfecting foreign genes and having a replication capacity, is provided. A Sendai virus vector comprising a foreign gene can be constructed by inserting a foreign gene between the viral genes of the Sendai virus genome. These Sendai viruses have a replication capacity and express the foreign gene in transfected cells. The expression level of the foreign gene is higher towards the 3′ end of negative strand RNA, and especially, a high level of expression is obtained when the foreign gene is inserted before the NP gene, and between P gene and M gene. Conversely, the expression decreases towards 5′ end of negative strand RNA, and especially, a relatively low level of expression is obtained when the when the foreign gene is inserted between HN gene and L gene, and between F gene and HN gene. Thus, the vector of the invention enables the regulation of the expression level of a foreign gene. The vector is useful for gene therapy due to its safety, high gene transfer efficiency, and capacity to express a foreign gene at a high level.
摘要翻译：提供了能够外切基因并具有复制能力的副粘病毒载体。可以通过在仙台病毒基因组的病毒基因之间插入外来基因来构建包含外来基因的仙台病毒载体。这些仙台病毒具有复制能力，并在转染的细胞中表达外源基因。外源基因的表达水平高于负链RNA的3'末端，特别是当外源基因插入NP基因之前和P基因与M基因之间时，可获得高水平的表达。相反，表达量向负链RNA的5'端减少，特别是当外源基因插入到HN基因和L基因之间以及F基因和HN基因之间时，获得相对低的表达水平。因此，本发明的载体能够调节外源基因的表达水平。该载体由于其安全性，高基因转移效率和在高水平表达外源基因的能力而可用于基因治疗。

2. 发明申请

US20060216824A1 Paramyxovirus vector encoding ribozyme and utilization thereof 审中-公开
标题翻译：编码核酶的副粘病毒载体及其应用
公开(公告)号：US20060216824A1
公开(公告)日：2006-09-28
申请号：US10543734
申请日：2004-01-30
申请人： Tsuyoshi Tokusumi , Hiroshi Ban , Akihiro Iida , Mamoru Hasegawa
发明人： Tsuyoshi Tokusumi , Hiroshi Ban , Akihiro Iida , Mamoru Hasegawa
IPC分类号： C12N15/86
CPC分类号： C12N15/113 , A61K38/00 , C12N15/86 , C12N2310/111 , C12N2310/121 , C12N2760/18843 , C12N2800/30
摘要： The present invention provides paramyxoviral vectors encoding active ribozymes. The vectors of the present invention are suitable as vectors for gene therapy to express desired ribozymes in a broad range of tissues in vivo or ex vivo. The vectors of the present invention can be used in gene therapy for cancers and other diseases.
摘要翻译：本发明提供编码活性核酶的副粘病毒载体。本发明的载体适合用作基因治疗的载体，以在体内或离体的宽范围的组织中表达所需的核酶。本发明的载体可用于癌症和其它疾病的基因治疗。

3. 发明授权

US07226786B2 Envelope gene-deficient Paramyxovirus vector 有权
标题翻译：包膜基因缺陷型副粘病毒载体
公开(公告)号：US07226786B2
公开(公告)日：2007-06-05
申请号：US10316535
申请日：2002-12-10
申请人： Kaio Kitazato , Tsugumine Shu , Hidekazu Kuma , Yasuji Ueda , Makoto Asakawa , Mamoru Hasegawa , Akihiro Iida , Fumino Tokito , Takahiro Hirata , Tsuyoshi Tokusumi , Makoto Inoue , Yumiko Tokusumi
发明人： Kaio Kitazato , Tsugumine Shu , Hidekazu Kuma , Yasuji Ueda , Makoto Asakawa , Mamoru Hasegawa , Akihiro Iida , Fumino Tokito , Takahiro Hirata , Tsuyoshi Tokusumi , Makoto Inoue , Yumiko Tokusumi
IPC分类号： C12N15/00 , C12N15/86
CPC分类号： C12N15/86 , A61K48/00 , A61K2039/5254 , A61K2039/5256 , C12N7/00 , C12N2760/18823 , C12N2760/18843 , C12N2760/18845 , C12N2760/18861 , C12N2800/30 , C12N2810/6081
摘要： F gene-deficient virus virions are successfully recovered by using an F gene-deficient Sendai virus genomic cDNA. Further, F gene-deficient infectious viral particles are successfully constructed by using F-expressing cells as helper cells. Also, F gene and HN gene-deficient virus virions are successfully recovered by using a virus genomic cDNA deficient in both F gene and HN gene. Further, F gene and HN gene-deficient infectious viral particles are successfully produced by using F- and HN-expressing cells as helper cells. A virus deficient in F gene and HN gene and having F protein is constructed by using F-expressing cells as helper cells. In addition, M gene-deficient infectious virus particles were produced using helper cells expressing M protein. From cells infected with M gene-deficient viruses, release of virus-like particles was inhibited. Further, a VSV-G pseudo type virus is successfully constructed by using VSV-G-expressing cells. Techniques for constructing these deficient viruses contribute to the development of vectors of Paramyxoviridae usable in gene therapy.
摘要翻译：通过使用F基因缺陷的仙台病毒基因组cDNA成功地回收了F基因缺陷型病毒粒子。此外，通过使用F表达细胞作为辅助细胞，成功构建了F基因缺陷型感染性病毒颗粒。此外，通过使用F基因和HN基因缺陷的病毒基因组cDNA，成功地回收了F基因和HN基因缺陷型病毒粒子。此外，通过使用F-和HN表达细胞作为辅助细胞，F基因和HN基因缺陷型感染性病毒颗粒成功产生。通过使用F表达细胞作为辅助细胞构建F基因和HN基因并具有F蛋白的病毒。此外，使用表达M蛋白的辅助细胞产生M基因缺陷型感染性病毒颗粒。从感染M基因缺陷病毒的细胞中，病毒样颗粒的释放被抑制。此外，通过使用表达VSV-G的细胞成功地构建了VSV-G伪型病毒。用于构建这些缺陷病毒的技术有助于可用于基因治疗的副粘病毒科的载体的发育。

4. 发明授权

US08741650B2 Methods for producing minus-strand RNA viral vectors using hybrid promoter comprising cytomegalovirus enhancer and chicken β-actin promoter 有权
标题翻译：使用包含巨细胞病毒增强子和鸡和肌动蛋白启动子的混合启动子产生负链RNA病毒载体的方法
公开(公告)号：US08741650B2
公开(公告)日：2014-06-03
申请号：US10586142
申请日：2005-01-20
申请人： Akihiro Iida , Hiroshi Ban , Makoto Inoue , Takahiro Hirata , Mamoru Hasegawa
发明人： Akihiro Iida , Hiroshi Ban , Makoto Inoue , Takahiro Hirata , Mamoru Hasegawa
IPC分类号： C12N15/86
CPC分类号： C12N15/86 , C12N2770/36143 , C12N2800/108 , C12N2800/30 , C12N2830/00 , C12N2830/15 , C12N2830/42 , C12N2830/90
摘要： The present invention provides methods for producing a minus-strand RNA viral vector, which comprise using a promoter comprising a cytomegalovirus enhancer and a chicken β-actin promoter, to induce the transcription of the genome RNA of a minus-strand RNA viral vector and the expression of minus-strand RNA viral proteins that form a ribonucleoprotein with the genome RNA. The methods of the present invention enable high efficiency production of highly safe minus-strand RNA viral vectors. The methods of the present invention are particularly useful for producing minus-strand RNA viral vectors that are deficient in envelope-constituting protein genes.
摘要翻译：本发明提供了用于产生负链RNA病毒载体的方法，其包括使用包含巨细胞病毒增强子和鸡和肌动蛋白启动子的启动子，以诱导负链RNA病毒载体的基因组RNA的转录，以及与基因组RNA形成核糖核蛋白的负链RNA病毒蛋白的表达。本发明的方法能高效生产高度安全的负链RNA病毒载体。本发明的方法特别可用于产生缺失包膜蛋白基因的负链RNA病毒载体。

5. 发明申请

US20070161110A1 Methods for producing minus-strand rna viral vectors using hybrid promoter comprising cytomegalovirus enhancer and chicken beta-actin promoter 有权
标题翻译：使用包含巨细胞病毒增强子和鸡β-肌动蛋白启动子的杂合启动子产生负链rna病毒载体的方法
公开(公告)号：US20070161110A1
公开(公告)日：2007-07-12
申请号：US10586142
申请日：2005-01-20
申请人： Akihiro Iida , Hiroshi Ban , Makoto Inoue , Takahiro Hirata , Mamoru Hasegawa
发明人： Akihiro Iida , Hiroshi Ban , Makoto Inoue , Takahiro Hirata , Mamoru Hasegawa
IPC分类号： C12N15/86 , C07H21/04 , C12N7/00
CPC分类号： C12N15/86 , C12N2770/36143 , C12N2800/108 , C12N2800/30 , C12N2830/00 , C12N2830/15 , C12N2830/42 , C12N2830/90
摘要： The present invention provides methods for producing a minus-strand RNA viral vector, which comprise using a promoter comprising a cytomegalovirus enhancer and a chicken β-actin promoter, to induce the transcription of the genome RNA of a minus-strand RNA viral vector and the expression of minus-strand RNA viral proteins that form a ribonucleoprotein with the genome RNA. The methods of the present invention enable high efficiency production of highly safe minus-strand RNA viral vectors. The methods of the present invention are particularly useful for producing minus-strand RNA viral vectors that are deficient in envelope-constituting protein genes.
摘要翻译：本发明提供了产生负链RNA病毒载体的方法，其包括使用包含巨细胞病毒增强子和鸡β-肌动蛋白启动子的启动子来诱导负链RNA病毒载体的基因组RNA的转录，表达与基因组RNA形成核糖核蛋白的负链RNA病毒蛋白。本发明的方法能高效生产高度安全的负链RNA病毒载体。本发明的方法特别可用于产生缺失包膜蛋白基因的负链RNA病毒载体。

6. 发明申请

US20070141705A1 Method for producing viral vectors 审中-公开
标题翻译：生产病毒载体的方法
公开(公告)号：US20070141705A1
公开(公告)日：2007-06-21
申请号：US10587123
申请日：2005-01-20
申请人： Makoto Inoue , Hiroshi Ban , Akihiro Iida , Mamoru Hasegawa
发明人： Makoto Inoue , Hiroshi Ban , Akihiro Iida , Mamoru Hasegawa
IPC分类号： C12N5/08 , C12N7/01 , C12N15/86
CPC分类号： C12N7/00 , C12N2760/18851
摘要： The present invention provides methods for producing viruses, whose propagation depends on the cleavage of viral protein by a protease, in a manner independent of the protease. The methods of the present invention for producing viruses comprise producing viruses in the presence of a modified viral protein in which the protease cleavage sequence is changed to a cleavage sequence for an alternative protease. Viral vectors can be more efficiently produced by replacing the protease cleavage sequence with a cleavage sequence for a protease expressed endogenously in virus-producing cells. The methods of the present invention enable the production of high titer viruses using a wide variety of cells.
摘要翻译：本发明提供了以与蛋白酶无关的方式生产病毒的方法，其传播依赖于蛋白酶对病毒蛋白的切割。用于产生病毒的本发明的方法包括在修饰的病毒蛋白质的存在下产生病毒，其中将蛋白酶切割序列改变为用于替代性蛋白酶的切割序列。通过用在病毒产生细胞内源性表达的蛋白酶的切割序列替换蛋白酶切割序列可以更有效地产生病毒载体。本发明的方法能够使用多种细胞产生高滴度病毒。

7. 发明申请

US20050130123A1 Methods of examining (-) strand rna virus vectors having lowered ability to form grains and method of constructing the same 审中-公开
标题翻译：检查具有降低形成颗粒能力的（ - ）链rna病毒载体的方法及其构建方法
公开(公告)号：US20050130123A1
公开(公告)日：2005-06-16
申请号：US10489384
申请日：2002-09-18
申请人： Makoto Inoue , Yumiko Tokusumi , Akihiro Iida , Mamoru Hasegawa
发明人： Makoto Inoue , Yumiko Tokusumi , Akihiro Iida , Mamoru Hasegawa
IPC分类号： A61K48/00 , C12N7/01 , C12N7/04 , C12N15/45 , C12N15/86 , C12Q1/70 , C12N15/867 , C12Q1/68
CPC分类号： C12N7/00 , A61K48/00 , C12N15/86 , C12N2760/18843 , C12N2760/18861 , C12N2800/30
摘要： The present invention provides methods for testing and producing (−) strand RNA virus vectors with reduced or eliminated particle formation ability or cytotoxicity. It was revealed that a deficiency in M protein localization in cells introduced with such a (−) strand RNA virus vector could result in the suppression of virus-like particle (VLP) formation in the cells. The present invention provides methods for testing and screening for a (−) strand RNA virus vector in which particle formation ability has been reduced or eliminated, and methods for producing a recombinant (−) strand RNA virus vector in which particle formation ability has been reduced or eliminated. Such a vector, in which VLP formation has been reduced or eliminated, is extremely useful as a vector for gene therapy, since it neither induces cytotoxicity nor immune response due to the secondary release of viruses from cells in which it has been introduced.
摘要翻译：本发明提供了测试和生产（ - ）链RNA病毒载体的方法，其具有降低或消除的颗粒形成能力或细胞毒性。显示出用这种（ - ）链RNA病毒载体引入的细胞中M蛋白定位的缺陷可导致细胞中病毒样颗粒（VLP）形成的抑制。本发明提供了用于测定和筛选其中减少或消除了颗粒形成能力的（ - ）链RNA病毒载体的方法，以及用于生产其中颗粒形成能力已经降低的重组（ - ）链RNA病毒载体的方法或消除。已经减少或消除VLP形成的这种载体作为用于基因治疗的载体是非常有用的，因为它既不引起细胞毒性也不诱导免疫应答，这是由于病毒从其引入细胞的二次释放引起的。

8. 发明申请

US20050221292A1 Vectors with modified protease-dependent tropism 有权
标题翻译：具有修饰的蛋白酶依赖性向量的载体
公开(公告)号：US20050221292A1
公开(公告)日：2005-10-06
申请号：US10513094
申请日：2003-04-30
申请人： Hiroaki Kinoh , Makoto Inoue , Yasuji Ueda , Akihiro Iida , Mamoru Hasegawa , Masanori Kobayashi
发明人： Hiroaki Kinoh , Makoto Inoue , Yasuji Ueda , Akihiro Iida , Mamoru Hasegawa , Masanori Kobayashi
IPC分类号： A61K38/00 , A61K48/00 , A61P35/00 , C07K14/115 , C12N15/45 , C12N15/86 , C12Q1/70 , C12Q1/68
CPC分类号： C07K14/005 , A61K38/00 , A61K48/00 , C07K2319/50 , C12N15/86 , C12N2760/18022 , C12N2760/18822 , C12N2760/18843 , C12N2800/30
摘要： The present invention provides cell fusogenic vectors having replicative ability, whose protease-dependent tropism has been modified. M gene-deficient viral vectors encoding modified F proteins, in which the cleavage site of the F protein of paramyxovirus is modified to be cleaved by different proteases, were produced. In cells transfected with these vectors, the genomic RNA present in the vectors is replicated, and cell fusogenic infection spreads to neighboring cells depending on the presence of other proteases; however, no viral particles are released. The vectors of this invention, encoding the F proteins which are cleaved by proteases whose activity is enhanced in cancer, show cancer growth suppressive effect in vivo.
摘要翻译：本发明提供具有复制能力的细胞融合载体，其蛋白酶依赖性向性已被修饰。产生编码修饰的F蛋白的M基因缺陷型病毒载体，其中修饰了被不同蛋白酶切割的副粘病毒的F蛋白的切割位点。在用这些载体转染的细胞中，载体中存在的基因组RNA被复制，并且根据其它蛋白酶的存在，细胞融合感染扩散到相邻细胞; 然而，没有病毒颗粒被释放。本发明的编码由蛋白酶切割的F蛋白的载体，其活性在癌症中增强，在体内显示出癌症生长抑制作用。

9. 发明授权

US08278284B2 Therapeutic agents for diseases associated with apoptotic degeneration in ocular tissue cells that use SIV-PEDF vectors 有权
标题翻译：与使用SIV-PEDF载体的眼组织细胞凋亡变性相关疾病的治疗剂
公开(公告)号：US08278284B2
公开(公告)日：2012-10-02
申请号：US11884738
申请日：2006-02-21
申请人： Masanori Miyazaki , Yoshikazu Yonemitsu , Yasuhiro Ikeda , Katsuo Sueishi , Toshiaki Tabata , Akihiro Iida , Yasuji Ueda , Mamoru Hasegawa
发明人： Masanori Miyazaki , Yoshikazu Yonemitsu , Yasuhiro Ikeda , Katsuo Sueishi , Toshiaki Tabata , Akihiro Iida , Yasuji Ueda , Mamoru Hasegawa
IPC分类号： A61K48/00 , A01N63/00 , C12P19/34
CPC分类号： A61K48/005 , A61K48/00 , C07K14/475 , C12N15/86 , C12N2740/15043 , C12N2740/15045 , C12N2810/6081
摘要： The present invention provides novel methods for treating diseases associated with apoptotic degeneration in ocular tissue cells by effective administration of pigment epithelium derived factor (PEDF). The present inventors studied PEDF as a means to prevent ganglion cell death, the final pathology of glaucoma. The present invention is particularly focused on SIV vectors for effective methods for delivering PEDF, and constructed an SIV-PEDF vector. When the SIV-PEDF vector was administered subretinally to an ischemia reperfusion model and NMDA-induced model, a significant suppression effect on ganglion cell death was observed. The present inventors therefore discovered that the SIV-PEDF vector is an effective pharmaceutical agent for treating diseases associated with apoptotic degeneration in ocular tissue cells, such as glaucoma.
摘要翻译：本发明提供了通过有效施用色素上皮衍生因子（PEDF）来治疗与眼组织细胞凋亡变性相关的疾病的新方法。本发明人研究了PEDF作为预防神经节细胞死亡，青光眼的最终病理学的手段。本发明特别关注用于递送PEDF的有效方法的SIV载体，并构建了SIV-PEDF载体。当将SIV-PEDF载体视觉上给予缺血再灌注模型和NMDA诱导的模型时，观察到对神经节细胞死亡的显着抑制作用。因此本发明人发现SIV-PEDF载体是用于治疗与眼组织细胞如青光眼中凋亡变性相关的疾病的有效药剂。

10. 发明申请

US20090233988A1 Therapeutic Agents for Diseases Associated With Apoptotic Degeneration in Ocular Tissue Cells That Use SIV-PEDF Vectors 有权
标题翻译：与使用SIV-PEDF载体的组织细胞凋亡相关的疾病治疗剂
公开(公告)号：US20090233988A1
公开(公告)日：2009-09-17
申请号：US11884738
申请日：2006-02-21
申请人： Masanori Miyazaki , Yoshikazu Yonemitsu , Yasuhiro Ikeda , Katsuo Sueishi , Toshiaki Tabata , Akihiro Iida , Yasuji Ueda , Mamoru Hasegawa
发明人： Masanori Miyazaki , Yoshikazu Yonemitsu , Yasuhiro Ikeda , Katsuo Sueishi , Toshiaki Tabata , Akihiro Iida , Yasuji Ueda , Mamoru Hasegawa
IPC分类号： A61K31/711 , C12N15/63 , C12N7/01
CPC分类号： A61K48/005 , A61K48/00 , C07K14/475 , C12N15/86 , C12N2740/15043 , C12N2740/15045 , C12N2810/6081
摘要： The present invention provides novel methods for treating diseases associated with apoptotic degeneration in ocular tissue cells by effective administration of pigment epithelium derived factor (PEDF). The present inventors studied PEDF as a means to prevent ganglion cell death, the final pathology of glaucoma. The present invention is particularly focused on SIV vectors for effective methods for delivering PEDF, and constructed an SIV-PEDF vector. When the SIV-PEDF vector was administered subretinally to an ischemia reperfusion model and NMDA-induced model, a significant suppression effect on ganglion cell death was observed. The present inventors therefore discovered that the SIV-PEDF vector is an effective pharmaceutical agent for treating diseases associated with apoptotic degeneration in ocular tissue cells, such as glaucoma.
摘要翻译：本发明提供了通过有效施用色素上皮衍生因子（PEDF）来治疗与眼组织细胞凋亡变性相关的疾病的新方法。本发明人研究了PEDF作为预防神经节细胞死亡，青光眼的最终病理学的手段。本发明特别关注用于递送PEDF的有效方法的SIV载体，并构建了SIV-PEDF载体。当将SIV-PEDF载体视觉上给予缺血再灌注模型和NMDA诱导的模型时，观察到对神经节细胞死亡的显着抑制作用。因此本发明人发现SIV-PEDF载体是用于治疗与眼组织细胞如青光眼中凋亡变性相关的疾病的有效药剂。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式