专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07163807B2 Nucleic acid sequences encoding modified prepro peptides of an alkaline protease 有权
标题翻译：编码碱性蛋白酶修饰前原肽的核酸序列
公开(公告)号：US07163807B2
公开(公告)日：2007-01-16
申请号：US11318576
申请日：2005-12-28
申请人： Tsuyoshi Sato , Mitsuyoshi Okuda , Yasushi Takimura , Nobuyuki Sumitomo , Masafumi Nomura , Tohru Kobayashi
发明人： Tsuyoshi Sato , Mitsuyoshi Okuda , Yasushi Takimura , Nobuyuki Sumitomo , Masafumi Nomura , Tohru Kobayashi
IPC分类号： C12N9/52 , C12N15/57 , C12N15/62 , C12N15/74
CPC分类号： C07H21/04 , C12N9/54
摘要： The present invention relates to genes expressing an alkaline protease having a prepro sequence, vectors containing the same, and transformants containing said vector. The present invention also relates to a method for producing an alkaline protease.
摘要翻译：本发明涉及表达具有前原序列的碱性蛋白酶，含有其的载体和含有该载体的转化体的基因。本发明还涉及碱性蛋白酶的制造方法。

2. 发明申请

US20060105428A1 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US20060105428A1
公开(公告)日：2006-05-18
申请号：US11318576
申请日：2005-12-28
申请人： Tsuyoshi Sato , Mitsuyoshi Okuda , Yasushi Takimura , Nobuyuki Sumitomo , Masafumi Nomura , Tohru Kobayashi
发明人： Tsuyoshi Sato , Mitsuyoshi Okuda , Yasushi Takimura , Nobuyuki Sumitomo , Masafumi Nomura , Tohru Kobayashi
IPC分类号： C12P21/06 , C12N9/64 , C07H21/04 , C12N15/74 , C12N1/21
CPC分类号： C07H21/04 , C12N9/54
摘要： The present invention relates to genes expressing an alkaline protease having a prepro sequence, vectors containing the same, and transformants containing said vector. The present invention also relates to a method for producing an alkaline protease.
摘要翻译：本发明涉及表达具有前原序列的碱性蛋白酶，含有其的载体和含有该载体的转化体的基因。本发明还涉及碱性蛋白酶的制造方法。

3. 发明授权

US07101698B2 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US07101698B2
公开(公告)日：2006-09-05
申请号：US10456479
申请日：2003-06-09
申请人： Tsuyoshi Sato , Mitsuyoshi Okuda , Yasushi Takimura , Nobuyuki Sumitomo , Masafumi Nomura , Tohru Kobayashi
发明人： Tsuyoshi Sato , Mitsuyoshi Okuda , Yasushi Takimura , Nobuyuki Sumitomo , Masafumi Nomura , Tohru Kobayashi
IPC分类号： C12N9/54 , C12N15/74 , C12P21/00 , C07K14/32
CPC分类号： C07H21/04 , C12N9/54
摘要： The present invention relates to an alkaline protease having a prepro sequence, wherein the prepro sequence is a mutant sequence of the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having 80% or higher homology to the amino acid sequence of SEQ ID NO: 1, in which amino acid residues at (a) position 52, (b) position 75, and (c) position 142 of SEQ ID NO: 1, or amino acid residues at positions corresponding to these positions are substituted by the following amino acid residues: at position (a): aspartic acid or arginine, at position (b): alanine or arginine, and at position (c): lysine; and the alkaline protease, when in a mature form, has the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having a homology of 80% or higher to this amino acid sequence. The present invention also relates to, for example, a gene encoding the alkaline protease. According to the present invention, an alkaline protease increased in production can be produced. In particular, there can be efficiently produced an alkaline protease having an activity even in the presence of a fatty acid of high concentration and exhibiting excellent detergency against complex soils containing proteins and sebum.
摘要翻译：本发明涉及具有前序列的碱性蛋白酶，其中前序列是SEQ ID NO：1的氨基酸序列的突变序列或与SEQ ID NO：1的氨基酸序列具有80％以上同源性的氨基酸序列 SEQ ID NO：1的（a）位置52，（b）位置75和（c）位置142处的氨基酸残基或对应于这些位置的位置处的氨基酸残基被替换为SEQ ID NO：以下氨基酸残基：位置（a）：天冬氨酸或精氨酸，位置（b）：丙氨酸或精氨酸，和位置（c）：赖氨酸; 碱性蛋白酶以成熟形式具有SEQ ID NO：2的氨基酸序列或与该氨基酸序列同源性为80％以上的氨基酸序列。本发明还涉及例如编码碱性蛋白酶的基因。根据本发明，可以生产生产中增加的碱性蛋白酶。特别是，即使在高浓度的脂肪酸的存在下也能够有效地生成具有活性的碱性蛋白酶，并且对于含有蛋白质和皮脂的复杂土壤表现出优异的去污力。

4. 发明授权

US07429642B2 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US07429642B2
公开(公告)日：2008-09-30
申请号：US10820714
申请日：2004-04-09
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
IPC分类号： C07K1/00
CPC分类号： C12N9/54 , C11D3/386
摘要： An alkaline protease having an amino acid with one or more amino acid residues selected from among those located at (a) position 15, (b) position 16, (c) position 166, (d) position 167, (e) position 187, (f) position 346, and (g) position 405 of the amino acid sequence of SEQ No; 1, or at positions corresponding to these positions, are the following amino acid residues, respectively: (a): histidine, (b): threonine or glutamine, (c): glycine, (d): valine, (e): serine, (f): arginine, and (g); and a gene encoding the alkaline protease.
摘要翻译：一种具有一个或多个氨基酸残基的氨基酸的碱性蛋白酶，其选自位于（a）位置15，（b）位置16，（c）位置166，（d）位置167，（e）位置187，（f）位置346和（g）SEQ ID NO：1的氨基酸序列的位置405; 分别为：（a）：组氨酸，（b）：苏氨酸或谷氨酰胺，（c）：甘氨酸，（d）：缬氨酸，（e））：丝氨酸，（f）：精氨酸和（g）; 和编码碱性蛋白酶的基因。

5. 发明申请

US20050214922A1 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US20050214922A1
公开(公告)日：2005-09-29
申请号：US10820714
申请日：2004-04-09
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
IPC分类号： C12N15/09 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/54 , C12N9/64
CPC分类号： C12N9/54 , C11D3/386
摘要： An alkaline protease having an amino acid sequence, wherein one or more amino acid residues selected from those located at (a) position 15, (b) position 16, (c) position 166, (d) position 167, (e) position 187, (f) position 346, and (g) position 405 of the amino acid sequence of SEQ ID NO: 1, or at positions corresponding to these positions are the following amino acid residues, respectively: (a): histidine, (b): threonine or glutamine, (c): glycine, (d) valine, (e): serine, (f): arginine, and (g): aspartic acid; and a gene encoding the alkaline protease. The alkaline protease exhibits for example excellent detergency against complex soil, and improved protein secretion amount and specific activity, and attains enhanced productivity.
摘要翻译：一种具有氨基酸序列的碱性蛋白酶，其中选自位于（a）位置15，（b）位置16，（c）位置166，（d）位置167，（e）位置187的氨基酸残基中的一个或多个氨基酸残基，（f）位置346和（g）SEQ ID NO：1的氨基酸序列的位置405或对应于这些位置的位置分别是以下氨基酸残基：（a）：组氨酸，（b）：苏氨酸或谷氨酰胺，（c）：甘氨酸，（d）缬氨酸，（e）：丝氨酸，（f）：精氨酸，和（g）：天冬氨酸; 和编码碱性蛋白酶的基因。碱性蛋白酶表现出对复杂土壤的优异的去污力，以及提高的蛋白质分泌量和比活性，并且提高了生产率。

6. 发明授权

US07473544B2 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US07473544B2
公开(公告)日：2009-01-06
申请号：US11235249
申请日：2005-09-27
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
IPC分类号： C12N9/64 , C11D3/386
CPC分类号： C11D3/386 , C12N9/54
摘要： The present invention provides an alkaline protease exhibiting high detergency and productivity, which is obtained by further enhancing the detergency of an alkaline protease effective against complex dirt and enhancing the specific activity.
摘要翻译：本发明提供了通过进一步提高碱性蛋白酶对复杂污垢有效并提高比活性的去污力而获得的，具有高洗涤性和生产率的碱性蛋白酶。

7. 发明申请

US20060078978A1 Alkaline protease 有权
公开(公告)号：US20060078978A1
公开(公告)日：2006-04-13
申请号：US11235249
申请日：2005-09-27
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
IPC分类号： C12N9/64
CPC分类号： C11D3/386 , C12N9/54
摘要： The present invention provides an alkaline protease exhibiting high detergency and productivity, which is obtained by further enhancing the detergency of an alkaline protease effective against complex dirt and enhancing the specific activity.

8. 发明授权

US08389685B2 Vector encoding a plasmid replication protein and use thereof 有权
标题翻译：编码质粒复制蛋白的载体及其用途
公开(公告)号：US08389685B2
公开(公告)日：2013-03-05
申请号：US12934395
申请日：2009-03-24
申请人： Yasushi Takimura , Yoshifumi Izawa , Nobuyuki Sumitomo
发明人： Yasushi Takimura , Yoshifumi Izawa , Nobuyuki Sumitomo
IPC分类号： C07K1/00 , C12N1/00 , C12N1/20 , C12N15/00 , C12P21/06 , C12P19/34 , C07H21/04
CPC分类号： C12N15/67 , C07K14/32
摘要： To provide a plasmid vector having high productivity which can be used for large scale production of an industrially valuable and useful protein, and a transformant using the plasmid vector. A plasmid vector having DNA which encodes a plasmid replication protein in which one or more amino acid residues that are selected from (a) position 48, (b) position 262, (c) position 149 and (d) position 198 in the amino acid sequence represented by SEQ ID NO: 2 are substituted with (a) Ala, Gly, Thr, Arg, Glu, Asn or Gln, (b) Gly, Ser, Thr, Cys or Val, (c) Asn, and (d) Glu, respectively.
摘要翻译：提供可用于大规模生产工业上有价值且有用的蛋白质的高生产率的质粒载体，以及使用质粒载体的转化体。一种质粒载体，其具有编码质粒复制蛋白的质粒载体，其中一个或多个选自（a）位置48，（b）位置262，（c）位置149和（d）位置198的氨基酸残基由（a）Ala，Gly，Thr，Arg，Glu，Asn或Gln，（b）Gly，Ser，Thr，Cys或Val，（c）Asn和（d）代替SEQ ID NO： Glu。

9. 发明申请

US20110021751A1 Plasmid Vector 有权
标题翻译：质粒载体
公开(公告)号：US20110021751A1
公开(公告)日：2011-01-27
申请号：US12934395
申请日：2009-03-24
申请人： Yasushi Takimura , Yoshifumi Izawa , Nobuyuki Sumitomo
发明人： Yasushi Takimura , Yoshifumi Izawa , Nobuyuki Sumitomo
IPC分类号： C07K14/315 , C12N15/63 , C12N1/00 , C12P21/02
CPC分类号： C12N15/67 , C07K14/32
摘要： To provide a plasmid vector having high productivity which can be used for large scale production of an industrially valuable and useful protein, and a transformant using the plasmid vector. A plasmid vector having DNA which encodes a plasmid replication protein in which one or more amino acid residues that are selected from (a) position 48, (b) position 262, (c) position 149 and (d) position 198 in the amino acid sequence represented by SEQ ID NO: 2 are substituted with (a) Ala, Gly, Thr, Arg, Glu, Asn or Gln, (b) Gly, Ser, Thr, Cys or Val, (c) Asn, and (d) Glu, respectively.
摘要翻译：提供可用于大规模生产工业上有价值且有用的蛋白质的高生产率的质粒载体，以及使用质粒载体的转化体。一种质粒载体，其具有编码质粒复制蛋白的质粒载体，其中一个或多个选自（a）位置48，（b）位置262，（c）位置149和（d）位置198的氨基酸残基由（a）Ala，Gly，Thr，Arg，Glu，Asn或Gln，（b）Gly，Ser，Thr，Cys或Val，（c）Asn和（d）代替SEQ ID NO： Glu。

10. 发明申请

US20110217743A1 Method of Producing Lauric Acid-containing Oil or Fat 审中-公开
标题翻译：生产含有月桂酸的油或脂肪的方法
公开(公告)号：US20110217743A1
公开(公告)日：2011-09-08
申请号：US12716608
申请日：2010-03-03
申请人： Hiroshi Yoshida , Fumikazu Takahashi , Yasushi Takimura
发明人： Hiroshi Yoshida , Fumikazu Takahashi , Yasushi Takimura
IPC分类号： C12P7/64
CPC分类号： C12P7/6463 , C12P7/6409
摘要： To provide a method for supplying lauric acid with algae.A method for producing an oil or fat containing 3 weight % or higher lauric acid in the fatty acid composition, by culturing an alga belonging to the genus Symbiodinium in a medium and recovering from the culture.
摘要翻译：提供一种向藻类提供月桂酸的方法。一种在脂肪酸组合物中含有3重量％以上月桂酸的油脂的制造方法，其特征在于，在培养基中培养属于所述共生菌属的藻类，从所述培养物中回收。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式