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1. 发明申请

US20060105428A1 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US20060105428A1
公开(公告)日：2006-05-18
申请号：US11318576
申请日：2005-12-28
申请人： Tsuyoshi Sato , Mitsuyoshi Okuda , Yasushi Takimura , Nobuyuki Sumitomo , Masafumi Nomura , Tohru Kobayashi
发明人： Tsuyoshi Sato , Mitsuyoshi Okuda , Yasushi Takimura , Nobuyuki Sumitomo , Masafumi Nomura , Tohru Kobayashi
IPC分类号： C12P21/06 , C12N9/64 , C07H21/04 , C12N15/74 , C12N1/21
CPC分类号： C07H21/04 , C12N9/54
摘要： The present invention relates to genes expressing an alkaline protease having a prepro sequence, vectors containing the same, and transformants containing said vector. The present invention also relates to a method for producing an alkaline protease.
摘要翻译：本发明涉及表达具有前原序列的碱性蛋白酶，含有其的载体和含有该载体的转化体的基因。本发明还涉及碱性蛋白酶的制造方法。

2. 发明授权

US07163807B2 Nucleic acid sequences encoding modified prepro peptides of an alkaline protease 有权
标题翻译：编码碱性蛋白酶修饰前原肽的核酸序列
公开(公告)号：US07163807B2
公开(公告)日：2007-01-16
申请号：US11318576
申请日：2005-12-28
申请人： Tsuyoshi Sato , Mitsuyoshi Okuda , Yasushi Takimura , Nobuyuki Sumitomo , Masafumi Nomura , Tohru Kobayashi
发明人： Tsuyoshi Sato , Mitsuyoshi Okuda , Yasushi Takimura , Nobuyuki Sumitomo , Masafumi Nomura , Tohru Kobayashi
IPC分类号： C12N9/52 , C12N15/57 , C12N15/62 , C12N15/74
CPC分类号： C07H21/04 , C12N9/54
摘要： The present invention relates to genes expressing an alkaline protease having a prepro sequence, vectors containing the same, and transformants containing said vector. The present invention also relates to a method for producing an alkaline protease.
摘要翻译：本发明涉及表达具有前原序列的碱性蛋白酶，含有其的载体和含有该载体的转化体的基因。本发明还涉及碱性蛋白酶的制造方法。

3. 发明授权

US07101698B2 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US07101698B2
公开(公告)日：2006-09-05
申请号：US10456479
申请日：2003-06-09
申请人： Tsuyoshi Sato , Mitsuyoshi Okuda , Yasushi Takimura , Nobuyuki Sumitomo , Masafumi Nomura , Tohru Kobayashi
发明人： Tsuyoshi Sato , Mitsuyoshi Okuda , Yasushi Takimura , Nobuyuki Sumitomo , Masafumi Nomura , Tohru Kobayashi
IPC分类号： C12N9/54 , C12N15/74 , C12P21/00 , C07K14/32
CPC分类号： C07H21/04 , C12N9/54
摘要： The present invention relates to an alkaline protease having a prepro sequence, wherein the prepro sequence is a mutant sequence of the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having 80% or higher homology to the amino acid sequence of SEQ ID NO: 1, in which amino acid residues at (a) position 52, (b) position 75, and (c) position 142 of SEQ ID NO: 1, or amino acid residues at positions corresponding to these positions are substituted by the following amino acid residues: at position (a): aspartic acid or arginine, at position (b): alanine or arginine, and at position (c): lysine; and the alkaline protease, when in a mature form, has the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having a homology of 80% or higher to this amino acid sequence. The present invention also relates to, for example, a gene encoding the alkaline protease. According to the present invention, an alkaline protease increased in production can be produced. In particular, there can be efficiently produced an alkaline protease having an activity even in the presence of a fatty acid of high concentration and exhibiting excellent detergency against complex soils containing proteins and sebum.
摘要翻译：本发明涉及具有前序列的碱性蛋白酶，其中前序列是SEQ ID NO：1的氨基酸序列的突变序列或与SEQ ID NO：1的氨基酸序列具有80％以上同源性的氨基酸序列 SEQ ID NO：1的（a）位置52，（b）位置75和（c）位置142处的氨基酸残基或对应于这些位置的位置处的氨基酸残基被替换为SEQ ID NO：以下氨基酸残基：位置（a）：天冬氨酸或精氨酸，位置（b）：丙氨酸或精氨酸，和位置（c）：赖氨酸; 碱性蛋白酶以成熟形式具有SEQ ID NO：2的氨基酸序列或与该氨基酸序列同源性为80％以上的氨基酸序列。本发明还涉及例如编码碱性蛋白酶的基因。根据本发明，可以生产生产中增加的碱性蛋白酶。特别是，即使在高浓度的脂肪酸的存在下也能够有效地生成具有活性的碱性蛋白酶，并且对于含有蛋白质和皮脂的复杂土壤表现出优异的去污力。

4. 发明授权

US07429642B2 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US07429642B2
公开(公告)日：2008-09-30
申请号：US10820714
申请日：2004-04-09
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
IPC分类号： C07K1/00
CPC分类号： C12N9/54 , C11D3/386
摘要： An alkaline protease having an amino acid with one or more amino acid residues selected from among those located at (a) position 15, (b) position 16, (c) position 166, (d) position 167, (e) position 187, (f) position 346, and (g) position 405 of the amino acid sequence of SEQ No; 1, or at positions corresponding to these positions, are the following amino acid residues, respectively: (a): histidine, (b): threonine or glutamine, (c): glycine, (d): valine, (e): serine, (f): arginine, and (g); and a gene encoding the alkaline protease.
摘要翻译：一种具有一个或多个氨基酸残基的氨基酸的碱性蛋白酶，其选自位于（a）位置15，（b）位置16，（c）位置166，（d）位置167，（e）位置187，（f）位置346和（g）SEQ ID NO：1的氨基酸序列的位置405; 分别为：（a）：组氨酸，（b）：苏氨酸或谷氨酰胺，（c）：甘氨酸，（d）：缬氨酸，（e））：丝氨酸，（f）：精氨酸和（g）; 和编码碱性蛋白酶的基因。

5. 发明申请

US20050214922A1 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US20050214922A1
公开(公告)日：2005-09-29
申请号：US10820714
申请日：2004-04-09
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
IPC分类号： C12N15/09 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/54 , C12N9/64
CPC分类号： C12N9/54 , C11D3/386
摘要： An alkaline protease having an amino acid sequence, wherein one or more amino acid residues selected from those located at (a) position 15, (b) position 16, (c) position 166, (d) position 167, (e) position 187, (f) position 346, and (g) position 405 of the amino acid sequence of SEQ ID NO: 1, or at positions corresponding to these positions are the following amino acid residues, respectively: (a): histidine, (b): threonine or glutamine, (c): glycine, (d) valine, (e): serine, (f): arginine, and (g): aspartic acid; and a gene encoding the alkaline protease. The alkaline protease exhibits for example excellent detergency against complex soil, and improved protein secretion amount and specific activity, and attains enhanced productivity.
摘要翻译：一种具有氨基酸序列的碱性蛋白酶，其中选自位于（a）位置15，（b）位置16，（c）位置166，（d）位置167，（e）位置187的氨基酸残基中的一个或多个氨基酸残基，（f）位置346和（g）SEQ ID NO：1的氨基酸序列的位置405或对应于这些位置的位置分别是以下氨基酸残基：（a）：组氨酸，（b）：苏氨酸或谷氨酰胺，（c）：甘氨酸，（d）缬氨酸，（e）：丝氨酸，（f）：精氨酸，和（g）：天冬氨酸; 和编码碱性蛋白酶的基因。碱性蛋白酶表现出对复杂土壤的优异的去污力，以及提高的蛋白质分泌量和比活性，并且提高了生产率。

6. 发明授权

US07473544B2 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US07473544B2
公开(公告)日：2009-01-06
申请号：US11235249
申请日：2005-09-27
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
IPC分类号： C12N9/64 , C11D3/386
CPC分类号： C11D3/386 , C12N9/54
摘要： The present invention provides an alkaline protease exhibiting high detergency and productivity, which is obtained by further enhancing the detergency of an alkaline protease effective against complex dirt and enhancing the specific activity.
摘要翻译：本发明提供了通过进一步提高碱性蛋白酶对复杂污垢有效并提高比活性的去污力而获得的，具有高洗涤性和生产率的碱性蛋白酶。

7. 发明申请

US20060078978A1 Alkaline protease 有权
公开(公告)号：US20060078978A1
公开(公告)日：2006-04-13
申请号：US11235249
申请日：2005-09-27
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
IPC分类号： C12N9/64
CPC分类号： C11D3/386 , C12N9/54
摘要： The present invention provides an alkaline protease exhibiting high detergency and productivity, which is obtained by further enhancing the detergency of an alkaline protease effective against complex dirt and enhancing the specific activity.

8. 发明授权

US07776578B2 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US07776578B2
公开(公告)日：2010-08-17
申请号：US12049022
申请日：2008-03-14
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Kazuhiro Saito , Nobuyuki Sumitomo , Yoshifumi Izawa , Katsuhisa Saeki , Tohru Kobayashi , Masafumi Nomura
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Kazuhiro Saito , Nobuyuki Sumitomo , Yoshifumi Izawa , Katsuhisa Saeki , Tohru Kobayashi , Masafumi Nomura
IPC分类号： C12N9/50 , C11D3/386 , C12N9/54 , C12N15/74
CPC分类号： C11D3/386 , C12N9/54 , C12Y304/21062
摘要： Provided in the present invention is an alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 170, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO:1 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (c): histidine, aspartic acid, phenylalanine, lysine, asparagine, serine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (f): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): serine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine.The present invention makes it possible to efficiently produce and provide alkaline proteases having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore being useful as an enzyme to be incorporated in a detergent.
摘要翻译：在本发明中提供的是碱性蛋白酶，其中（a）位置65，（b）位置101，（c）位置163，（d）位置170，（e）位置171，（f），（g）位置320，（h）位置359或（i）SEQ ID NO：位置（a）：脯氨酸，位置（b）：天冬酰胺，位置（c）：组氨酸，天冬氨酸，苯丙氨酸，赖氨酸，天冬酰胺，位置（d）：缬氨酸和亮氨酸，位置（e）：丙氨酸，谷氨酸，甘氨酸和苏氨酸，位置（f）：异亮氨酸，甘氨酸和苏氨酸，位置（g）位置（h）：丝氨酸，亮氨酸，缬氨酸，异亮氨酸和谷氨酰胺，位置（i）：丙氨酸，赖氨酸，谷氨酰胺，谷氨酸，精氨酸和组氨酸，位置（h）：缬氨酸，缬氨酸，苏氨酸，亮氨酸，异亮氨酸和甘氨酸。本发明使得可以有效地生产和提供具有活性的碱性蛋白酶，即使在高度浓缩的脂肪酸的存在下也具有优异的去污力来去除含有蛋白质，皮脂等的复合污渍，因此可用作要掺入洗涤剂中的酶。

9. 发明授权

US07368273B2 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US07368273B2
公开(公告)日：2008-05-06
申请号：US10385662
申请日：2003-03-12
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Kazuhiro Saito , Nobuyuki Sumitomo , Yoshifumi Izawa , Katsuhisa Saeki , Tohru Kobayashi , Masafumi Nomura
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Kazuhiro Saito , Nobuyuki Sumitomo , Yoshifumi Izawa , Katsuhisa Saeki , Tohru Kobayashi , Masafumi Nomura
IPC分类号： C12N9/48 , C11D3/386
CPC分类号： C11D3/386 , C12N9/54 , C12Y304/21062
摘要： An alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 17-0, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO: 2 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (e): histidine, aspartic acid, phenylalanine, lysine, asparagine, seine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (K): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): seine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine. A method to an alkaline protease having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore useful as an ingredient in a detergent.
摘要翻译：（a）位置65，（b）位置101，（c）位置163，（d）位置17-0，（e）位置171，（f）位置273，（g）位置320，（h）位置359或（i）SEQ ID NO：位置（a）：脯氨酸，位置（b）：天冬酰胺，位置（e）：组氨酸，天冬氨酸，苯丙氨酸，赖氨酸，天冬酰胺，（e）：丙氨酸，谷氨酸，甘氨酸和苏氨酸，位置（K）：异亮氨酸，甘氨酸和苏氨酸，位置（g）：缬氨酸和亮氨酸，（i）：丙氨酸，赖氨酸，谷氨酰胺，谷氨酸，精氨酸和组氨酸，位置（h）：ine碱，亮氨酸，缬氨酸，异亮氨酸和谷氨酰胺。一种碱性蛋白酶的方法，即使在高度浓缩的脂肪酸的存在下也具有活性，并且对于去除含有蛋白质，皮脂等的复合污渍具有优异的去污力，因此可用作洗涤剂中的成分。

10. 发明申请

US20080177040A1 ALKALINE PROTEASE 有权
标题翻译：碱性蛋白酶
公开(公告)号：US20080177040A1
公开(公告)日：2008-07-24
申请号：US12049022
申请日：2008-03-14
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Kazuhiro Saito , Nobuyuki Sumitomo , Yoshifumi Izawa , Katsuhisa Saeki , Tohru Kobayashi , Masafumi Nomura
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Kazuhiro Saito , Nobuyuki Sumitomo , Yoshifumi Izawa , Katsuhisa Saeki , Tohru Kobayashi , Masafumi Nomura
IPC分类号： C07K2/00
CPC分类号： C11D3/386 , C12N9/54 , C12Y304/21062
摘要： Provided in the present invention is an alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 170, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO:1 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (c): histidine, aspartic acid, phenylalanine, lysine, asparagine, serine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (f): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): serine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine.The present invention makes it possible to efficiently produce and provide alkaline proteases having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore being useful as an enzyme to be incorporated in a detergent.
摘要翻译：在本发明中提供的是碱性蛋白酶，其中（a）位置65，（b）位置101，（c）位置163，（d）位置170，（e）位置171，（f），（g）位置320，（h）位置359或（i）SEQ ID NO：位置（a）：脯氨酸，位置（b）：天冬酰胺，位置（c）：组氨酸，天冬氨酸，苯丙氨酸，赖氨酸，天冬酰胺，位置（d）：缬氨酸和亮氨酸，位置（e）：丙氨酸，谷氨酸，甘氨酸和苏氨酸，位置（f）：异亮氨酸，甘氨酸和苏氨酸，位置（g）位置（h）：丝氨酸，亮氨酸，缬氨酸，异亮氨酸和谷氨酰胺，位置（i）：丙氨酸，赖氨酸，谷氨酰胺，谷氨酸，精氨酸和组氨酸，位置（h）：缬氨酸，缬氨酸，苏氨酸，亮氨酸，异亮氨酸和甘氨酸。本发明使得可以有效地生产和提供具有活性的碱性蛋白酶，即使在高度浓缩的脂肪酸的存在下也具有优异的去污力来去除含有蛋白质，皮脂等的复合污渍，因此可用作要掺入洗涤剂中的酶。

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