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1. 发明授权

US09512171B2 Functional molecule and manufacturing method therefor 有权
标题翻译：功能分子及其制造方法
公开(公告)号：US09512171B2
公开(公告)日：2016-12-06
申请号：US12929359
申请日：2011-01-19
申请人： Tsuyoshi Fujihara , Tomoyasu Kichise , Shozo Fujita
发明人： Tsuyoshi Fujihara , Tomoyasu Kichise , Shozo Fujita
IPC分类号： C40B30/04 , C07K1/107 , C07K1/04 , C07K1/22
CPC分类号： C07K1/1077 , C07K1/04 , C07K1/22
摘要： A novel method for efficiently manufacturing a functional molecule with affinity for a target substance is provided along with a functional molecule manufactured by this method. The method for manufacturing a functional molecule includes binding, to a resin, a specific substance having a cleavable linker which is a site that is cleavable under a specific condition to prepare a specific-substance-bound resin, treating this specific-substance-bound resin with a solution comprising functional molecule candidates, and then cleaving the cleavable linker under the specific condition to select a functional molecule which has interacted specifically with the specific substance.
摘要翻译：与通过该方法制造的功能分子一起提供了对目标物质具有亲和性的功能性分子的有效制造的新颖方法。功能分子的制造方法包括将具有可切割接头的特定物质与特定物质结合的树脂结合，所述可切割接头是在特定条件下可切割的部位，处理该特定物质结合树脂用包含功能性分子候选物的溶液，然后在特定条件下切割可切割接头以选择与特异性物质特异性相互作用的功能性分子。

2. 发明申请

US20110151510A1 Functional molecule and manufacturing method therefor 有权
标题翻译：功能分子及其制造方法
公开(公告)号：US20110151510A1
公开(公告)日：2011-06-23
申请号：US12929359
申请日：2011-01-19
申请人： Tsuyoshi Fujihara , Tomoyasu Kichise , Shozo Fujita
发明人： Tsuyoshi Fujihara , Tomoyasu Kichise , Shozo Fujita
IPC分类号： C12P21/06
CPC分类号： C07K1/1077 , C07K1/04 , C07K1/22
摘要： A novel method for efficiently manufacturing a functional molecule with affinity for a target substance is provided along with a functional molecule manufactured by this method. The method for manufacturing a functional molecule includes binding, to a resin, a specific substance having a cleavable linker which is a site that is cleavable under a specific condition to prepare a specific-substance-bound resin, treating this specific-substance-bound resin with a solution comprising functional molecule candidates, and then cleaving the cleavable linker under the specific condition to select a functional molecule which has interacted specifically with the specific substance.
摘要翻译：与通过该方法制造的功能分子一起提供了对目标物质具有亲和性的功能性分子的有效制造的新颖方法。功能分子的制造方法包括将具有可切割接头的特定物质与特定物质结合的树脂结合，所述可切割接头是在特定条件下可切割的部位，处理该特定物质结合树脂用包含功能性分子候选物的溶液，然后在特定条件下切割可切割接头以选择与特异性物质特异性相互作用的功能性分子。

3. 发明申请

US20080214404A1 Functional molecule binding specifically to membrane protein, and manufacturing method therefor 审中-公开
标题翻译：功能性分子特异性结合膜蛋白，及其制造方法
公开(公告)号：US20080214404A1
公开(公告)日：2008-09-04
申请号：US12071336
申请日：2008-02-20
申请人： Tomoyasu Kichise , Tsuyoshi Fujihara , Shozo Fujita
发明人： Tomoyasu Kichise , Tsuyoshi Fujihara , Shozo Fujita
IPC分类号： C40B20/08 , C40B30/04
CPC分类号： C12Q1/6804
摘要： A membrane protein is brought into contact with a pool of functional molecule candidates including a modified oligonucleotide sequence obtained by random polymerization of modified nucleotide n-mers (wherein n is an integer) having a modified nucleoside having a substituent introduced therein, then the membrane protein is treated with a protease, and the functional molecule that has bound to the membrane protein is isolated. The corresponding unmodified molecule is amplified from this function molecule, the sequence of corresponding unmodified molecules obtained by the amplification is decoded, and the decoding results are translated into the sequence of the isolated functional molecule. The functional molecule can be manufactured based on this translation.
摘要翻译：使膜蛋白质与功能性分子候选物池接触，包括通过随机聚合修饰的核苷酸n-mers（其中n为整数）而获得的修饰的寡核苷酸序列，其具有引入其中的取代基的修饰的核苷，然后膜蛋白用蛋白酶处理，分离与膜蛋白结合的功能分子。从该功能分子扩增相应的未修饰的分子，对通过扩增获得的相应未修饰分子的序列进行解码，将解码结果转化为分离的功能分子的序列。功能分子可以基于这种翻译制造。

4. 发明申请

US20070232793A1 Functional molecule and manufacturing method therefor 审中-公开
公开(公告)号：US20070232793A1
公开(公告)日：2007-10-04
申请号：US11727390
申请日：2007-03-26
申请人： Tsuyoshi Fujihara , Tomoyasu Kichise , Shozo Fujita
发明人： Tsuyoshi Fujihara , Tomoyasu Kichise , Shozo Fujita
IPC分类号： C07K1/22 , C07H21/04
CPC分类号： C07K1/1077 , C07K1/04 , C07K1/22
摘要： A novel method for efficiently manufacturing a functional molecule with affinity for a target substance is provided along with a functional molecule manufactured by this method. The method for manufacturing a functional molecule includes binding, to a resin, a specific substance having a cleavable linker which is a site that is cleavable under a specific condition to prepare a specific-substance-bound resin, treating this specific-substance-bound resin with a solution comprising functional molecule candidates, and then cleaving the cleavable linker under the specific condition to select a functional molecule which has interacted specifically with the specific substance.

5. 发明授权

US07759473B2 Nucleotide oligomer, nucleotide polymer, method for determining structure of functional substance and method for manufacturing functional substance 有权
标题翻译：核苷酸寡聚物，核苷酸聚合物，功能性物质结构的测定方法及功能性物质的制造方法
公开(公告)号：US07759473B2
公开(公告)日：2010-07-20
申请号：US11352197
申请日：2006-02-13
申请人： Tsuyoshi Fujihara , Shozo Fujita
发明人： Tsuyoshi Fujihara , Shozo Fujita
IPC分类号： C07H21/02 , C07H21/04
CPC分类号： C07H21/00
摘要： A modified nucleotide n-mer (where n is an integer of 2 or more) is used which comprises a nucleoside unit with a substituent group introduced into the base, wherein the substituent group is bound to the base via a triple bond. Novel nucleotide oligomers, nucleotide polymers, and nucleosides which can be used as raw materials or intermediates in the synthesis of this nucleotide oligomer and nucleotide polymer, as well as novel techniques for structural determination and manufacture of a functional substance having high affinity for a target, are provided.
摘要翻译：使用修饰的核苷酸n-mer（其中n为2以上的整数），其包含具有引入碱中的取代基的核苷单元，其中所述取代基通过三键与碱结合。可用作合成该核苷酸寡聚体和核苷酸聚合物的原料或中间体的新型核苷酸低聚物，核苷酸聚合物和核苷，以及用于结构测定和制造对靶具有高亲和力的功能物质的新技术，被提供。

6. 发明申请

US20100144060A1 Protein detecting device 有权
标题翻译：蛋白检测装置
公开(公告)号：US20100144060A1
公开(公告)日：2010-06-10
申请号：US12654137
申请日：2009-12-11
申请人： Shozo Fujita , Shunsaku Takeishi , Tsuyoshi Fujihara
发明人： Shozo Fujita , Shunsaku Takeishi , Tsuyoshi Fujihara
IPC分类号： G01N33/543
CPC分类号： G01N33/5438 , G01N33/53 , G01N33/542 , G01N33/54353 , G01N33/68
摘要： A protein detecting device, which comprises: (1) a detecting unit having a bonding section, which has properties for specifically bonding to a protein to be detected, a detecting section for detecting the bonding of the protein to be detected to the bonding section, the detecting section being made up of a polynucleotide double strand and a charge separating group, and an electrode section detecting the change in electrical conductivity of, or amount of transferred charge in, the polynucleotide double strand modified by the bond of the protein, (2) a standard electrode, (3) a reference electrode, (4) a container for housing the detecting unit, the standard electrode and the reference electrode, and containing a sample solutions comprising the protein to be detected, and (5) a measuring unit for measuring the protein based on a signal detected in the detecting unit.
摘要翻译：一种蛋白质检测装置，其特征在于，包括：（1）检测单元，具有具有特异性地结合待检测蛋白质的接合部的检测部，检测所述被检测蛋白质与接合部的接合的检测部，检测部分由多核苷酸双链和电荷分离基团组成，电极部分检测由蛋白质键修饰的多核苷酸双链中的导电性或转移电荷量的变化（2 ）标准电极，（3）参考电极，（4）容纳检测单元，标准电极和参比电极的容器，并包含含有待检测蛋白质的样品溶液，和（5）测量单元用于基于在检测单元中检测到的信号测量蛋白质。

7. 发明授权

US07517646B2 Functional molecule and process for producing the same 有权
标题翻译：功能分子及其制备方法
公开(公告)号：US07517646B2
公开(公告)日：2009-04-14
申请号：US10943150
申请日：2004-09-17
申请人： Tsuyoshi Fujihara , Shozo Fujita , Shunsaku Takeishi
发明人： Tsuyoshi Fujihara , Shozo Fujita , Shunsaku Takeishi
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/00 , C07H21/02 , C07H19/04
CPC分类号： A61K31/7115 , A61K48/00 , C07H21/00 , C12N15/1048 , C12N15/1058 , C12N15/115 , C12N2310/53
摘要： A process for producing a functional molecule includes a forming step which forms a modified nucleotide n-mer (where, n represents an integer) containing a modified nucleoside prepared by introducing a substituent into a nucleoside composing a nucleic acid; and a producing step which produces a modified oligonucleotide sequence by randomly polymerizing the modified nucleotide n-mer. A preferable embodiment thereof includes a selecting step which selects a sequence having an affinity to a target from the modified oligonucleotide sequence, a determining step which amplifies the selected modified oligonucleotide sequence and determines the base sequence thereof, and a translating step which translates the sequence of the modified oligonucleotide sequence on the basis of a relation table prepared by relating at least one of 4n kinds of nucleotide n-mers, which are presented in the relation table prepared by the one-to-one combination of 4 kinds of nucleosides, to a modified nucleotide n-mer.
摘要翻译：制备功能性分子的方法包括：形成步骤，其形成含有通过将取代基引入构成核酸的核苷中制备的修饰核苷的修饰的核苷酸n-mer（其中，n表示整数）; 以及通过随机聚合修饰的核苷酸n-mer产生修饰的寡核苷酸序列的制备步骤。其优选实施方案包括从修饰的寡核苷酸序列中选择与靶具有亲和性的序列的选择步骤，放大所选择的修饰寡核苷酸序列并确定其碱基序列的确定步骤，以及翻译步骤，基于通过将通过将4种核苷的一对一组合制备的关系表中存在的4n种核苷酸n-mers中的至少一种与至少一种核苷酸n-mers相关联的关系表，修饰的寡核苷酸序列与修饰的核苷酸n-mer。

8. 发明申请

US20110100822A1 Device and method for quantitatively determining an analyte, a method for determining an effective size of a molecule, a method for attaching molecules to a substrate, and a device for detecting molecules 有权
标题翻译：用于定量测定分析物的装置和方法，确定分子的有效尺寸的方法，将分子附着于基底的方法以及用于检测分子的装置
公开(公告)号：US20110100822A1
公开(公告)日：2011-05-05
申请号：US12923588
申请日：2010-09-29
申请人： Kenji Arinaga , Ulrich Rant , Tsuyoshi Fujihara , Shozo Fujita
发明人： Kenji Arinaga , Ulrich Rant , Tsuyoshi Fujihara , Shozo Fujita
IPC分类号： G01N27/447 , C12Q1/37
CPC分类号： G01N33/5438 , B01J2219/00605 , C12Q2527/137 , C12Q2527/143 , C12Q2565/518 , G01N33/543
摘要： A device for quantitatively determining an analyte is provided to conspicuously improve the performance of the quantitative determination. This device is equipped with a flow channel, an analyte detecting unit for capturing and detecting the analyte, and a quantitative measurement unit for quantitatively determining the analyte, wherein a signal generated when the analyte detecting unit has detected the analyte is divided into a plurality of parts in the direction of the flow in the flow channel at the quantitative measurement unit for processing. Also provided are technologies including one for controlling the density of molecules attached to the surface of a solid. In these technologies, when molecules are attached to a substrate, the density of attached molecules is controlled, by having an electrolyte also present in a solution containing the molecules to adjust the screening effect by the electrolyte, and by taking into consideration the effective size of a molecule.
摘要翻译：提供了一种用于定量测定分析物的装置，以显着提高定量测定的性能。该装置配备有流路，用于捕获和检测分析物的分析物检测单元和用于定量测定分析物的定量测量单元，其中当分析物检测单元检测到分析物时产生的信号被分成多个在定量测量单元处的流动通道中的流动方向上的部件进行处理。还提供了包括用于控制附着在固体表面上的分子的密度的技术。在这些技术中，当分子连接到基底上时，通过在含有分子的溶液中也存在电解质来调节附着分子的密度，以调节电解质的筛选效果，并考虑到有效尺寸一个分子

9. 发明申请

US20080300393A1 Nucleotide oligomer, nucleotide polymer, method for determining structure of functional substance and method for manufacturing functional substance 有权
标题翻译：核苷酸寡聚物，核苷酸聚合物，功能性物质结构的测定方法及功能性物质的制造方法
公开(公告)号：US20080300393A1
公开(公告)日：2008-12-04
申请号：US11352197
申请日：2006-02-13
申请人： Tsuyoshi Fujihara , Shozo Fujita
发明人： Tsuyoshi Fujihara , Shozo Fujita
IPC分类号： C07H21/00
CPC分类号： C07H21/00
摘要： A modified nucleotide n-mer (where n is an integer of 2 or more) is used which comprises a nucleoside unit with a substituent group introduced into the base, wherein the substituent group is bound to the base via a triple bond. Novel nucleotide oligomers, nucleotide polymers, and nucleosides which can be used as raw materials or intermediates in the synthesis of this nucleotide oligomer and nucleotide polymer, as well as novel techniques for structural determination and manufacture of a functional substance having high affinity for a target, are provided.
摘要翻译：使用修饰的核苷酸n-mer（其中n为2以上的整数），其包含具有引入碱中的取代基的核苷单元，其中所述取代基通过三键与碱结合。可用作合成该核苷酸寡聚体和核苷酸聚合物的原料或中间体的新型核苷酸低聚物，核苷酸聚合物和核苷，以及用于结构测定和制造对靶具有高亲和力的功能物质的新技术，被提供。

10. 发明申请

US20060003437A1 Target detecting device and target capturer, device and method for molecular adsorption or desorption, and device and method for protein detection 审中-公开
标题翻译：目标检测装置和靶捕获装置，用于分子吸附或解吸的装置和方法，以及用于蛋白质检测的装置和方法
公开(公告)号：US20060003437A1
公开(公告)日：2006-01-05
申请号：US11223924
申请日：2005-09-13
申请人： Tsuyoshi Fujihara , Shozo Fujita , Shunsaku Takeishi , Kenji Arinaga , Yoshitaka Yamaguchi , Tatsuya Usuki
发明人： Tsuyoshi Fujihara , Shozo Fujita , Shunsaku Takeishi , Kenji Arinaga , Yoshitaka Yamaguchi , Tatsuya Usuki
IPC分类号： C12M3/00
CPC分类号： C12Q1/6816 , C12Q1/6825 , C12Q2565/607 , C12Q2565/107 , C12Q2523/308
摘要： The present invention provides, for example, a target detecting device comprising a target capturer, means for releasing the target capturer, light irradiating means and light detecting means, the target capturer at least partially containing a region interactive with an electrically conductive member, being capable of capturing a target, and being capable of emitting light upon irradiation with light in the case of not interacting with the electrically conductive member, the means for releasing the target capturer serving to release the target capturer from the electrically conductive member by ceasing the interaction between the target capturer and the electrically conductive member, the light irradiating means serving to apply light to the electrically conductive member, and the light detecting means serving to detect light emitted by the target capturer upon irradiation of light applied by the light irradiating means. It also provides a target capturer comprising an interacting section, a capturing section and a light emitting section, the interacting section at least partially containing a region interactive with an electrically conductive member, the capturing section capable of capturing a target, and the light emitting section capable of emitting light upon irradiation with light when the region in the interacting section does not interact with the electrically conductive member.
摘要翻译：本发明提供例如包括目标捕获器的目标检测装置，用于释放目标捕获器的装置，光照射装置和光检测装置，目标捕获器至少部分地包含与导电构件交互的区域，能够捕获目标，并且在不与导电构件相互作用的情况下能够在照射光时发光，所述用于释放目标捕获器的装置用于通过停止导电构件之间的相互作用而释放目标捕获器目标捕获器和导电构件，用于向导电构件施加光的光照射装置，以及用于在由照射装置施加的光照射时检测由目标捕获器发射的光的光检测装置。它还提供一种包括相互作用部分，捕获部分和发光部分的目标捕获器，所述相互作用部分至少部分地包含与导电部件交互的区域，所述捕获部分能够捕获目标，并且所述发光部分当相互作用部分中的区域不与导电构件相互作用时能够用光照射发光。

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