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1. 发明授权

US07078200B2 Method for manufacturing a polyhydroxyalkanoate 失效
标题翻译：聚羟基链烷酸酯的制造方法
公开(公告)号：US07078200B2
公开(公告)日：2006-07-18
申请号：US10603996
申请日：2003-06-26
申请人： Tsutomu Honma , Etsuko Sugawa , Tetsuya Yano , Takeshi Imamura , Takashi Kenmoku
发明人： Tsutomu Honma , Etsuko Sugawa , Tetsuya Yano , Takeshi Imamura , Takashi Kenmoku
IPC分类号： C12P7/62 , C08G63/06 , C08G63/78
CPC分类号： C12P7/625 , C08G63/06 , C08G63/6822 , C08G63/6852
摘要： A method for producing a polyhydroxyalkanoate using a microorganism capable of substantially reducing unintended monomer units and obtaining the polyhydroxyalkanoate in a high yield. A microorganism capable of synthesizing a novel polyhydroxyalkanoate having 3-hydroxy-substituted benzoylalkanoic acid as a monomer unit, using a substituted benzoylalkanoic acid as a material, is cultured in a medium containing a substituted benzoylalkanoic acid. Then, the polyhydroxyalkanoate produced in the cultured bacteria is extracted and recovered.
摘要翻译：使用能够显着降低非预期的单体单元并以高产率获得聚羟基链烷酸酯的微生物制备聚羟基链烷酸酯的方法。在含有取代的苯甲酰基链烷酸的培养基中培养能够以取代的苯甲酰基链烷酸为原料合成具有3-羟基取代的苯甲酰基链烷酸作为单体单元的新型聚羟基链烷酸酯的微生物。然后，提取并回收在培养细菌中产生的聚羟基链烷酸酯。

2. 发明授权

US06635782B2 Polyhydroxyalkanoate and manufacturing method thereof 失效
标题翻译：聚羟基链烷酸酯及其制造方法
公开(公告)号：US06635782B2
公开(公告)日：2003-10-21
申请号：US09951720
申请日：2001-09-14
申请人： Tsutomu Honma , Etsuko Sugawa , Tetsuya Yano , Takeshi Imamura , Takashi Kenmoku
发明人： Tsutomu Honma , Etsuko Sugawa , Tetsuya Yano , Takeshi Imamura , Takashi Kenmoku
IPC分类号： C07C6976
CPC分类号： C12P7/625 , C08G63/06 , C08G63/6822 , C08G63/6852
摘要： To provide a novel polyhydroxyalkanoate and a manufacturing method by a microorganism capable of substantially reducing unintended monomer units and obtaining the polyhydroxyalkanoate in a high yield. A microorganism capable of synthesizing a novel polyhydroxyalkanoate having 3-hydroxy-substituted benzoylalkanoic acid as a monomer unit using a substituted benzoylalkanoic acid as a material is cultured in the medium containing a substituted benzoylalkanoic acid, then the polyhydroxyalkanoate produced in the cultured bacteria is extracted and recovered.
摘要翻译：提供一种新型的聚羟基链烷酸酯及其制造方法，该微生物能够基本上减少非预期的单体单元并以高产率获得聚羟基链烷酸酯。在含有取代的苯甲酰基链烷酸的培养基中培养能够使用取代的苯甲酰基链烷酸合成具有3-羟基取代的苯甲酰基链烷酸作为单体单元的新型聚羟基链烷酸酯的微生物，然后提取培养细菌中产生的聚羟基链烷酸酯，恢复。

3. 发明授权

US07056708B2 Method of producing polyhydroxyalkanoate from alkane having residue containing aromatic ring in its molecule 失效
标题翻译：从其分子中含有芳环的残基的烷烃生产聚羟基链烷酸酯的方法
公开(公告)号：US07056708B2
公开(公告)日：2006-06-06
申请号：US10410349
申请日：2003-04-10
申请人： Takashi Kenmoku , Etsuko Sugawa , Tetsuya Yano , Takeshi Imamura , Tsutomu Honma
发明人： Takashi Kenmoku , Etsuko Sugawa , Tetsuya Yano , Takeshi Imamura , Tsutomu Honma
IPC分类号： C12P11/00 , C12P7/62
CPC分类号： C08G63/6882 , C12P7/625 , C12P11/00
摘要： The present invention provides a method of effectively producing polyhydroxyalkanoate having an aromatic substituted residue in its monomer unit. In the method of producing polyhydroxyalkanoate by using microorganisms, microorganisms capable of producing polyhydroxyalkanoate are cultured in a culture medium containing at least one starting compound selected from the group consisting of substituted alkanes represented by formula (1) to produce polyhydroxyalkanoate having, in its molecule, at least one unit selected from the group consisting of 3-hydroxy-substituted alkanoate units represented by formula (2): R—(CH2)n—CH2—CH2—CH3 (1) wherein R represents a residue containing a substituted aromatic ring, and n represents any integer of 1 to 8; wherein R represents a residue containing a substituted aromatic ring, and n represents any integer of 1 to 8. In formulas (1) and (2), a residue R is as described in the specification.
摘要翻译：本发明提供了在其单体单元中有效制备具有芳族取代残基的聚羟基链烷酸酯的方法。在通过使用微生物制备聚羟基链烷酸酯的方法中，能够在含有选自由式（1）表示的取代烷烃中的至少一种起始化合物的培养基中培养能够产生聚羟基链烷酸酯的微生物，从而产生在其分子中具有的聚羟基链烷酸酯，选自由式（2）表示的3-羟基取代的链烷酸酯单元中的至少一个单元：<？in-line-formula description =“In-line Formulas”end =“lead”？> R-（CH （2）-CH 2 CH 2 CH 2 CH 2（1）其中，R表示含有取代芳环的残基，n表示1〜8的整数，n表示1〜8的整数。其中R表示含有取代芳环的残基，n表示1至8的任何整数。在式（1）和（2）中，残基R如说明书所述。

4. 发明授权

US06777153B2 POLYHYDROXYALKANOATE CONTAINING UNIT WITH THIENYL STRUCTURE IN THE SIDE CHAIN, PROCESS FOR ITS PRODUCTION, CHARGE CONTROL AGENT, TONER BINDER AND TONER WHICH CONTAIN THIS POLYHYDROXYALKANOATE, AND IMAGE-FORMING METHOD AND IMAGE-FORMING APPARATUS WHICH MAKE USE OF THE TONER 失效
标题翻译：含有三链体结构的聚羟基烷基酯单元，其链中具有三烯结构，其制备方法，充电控制剂，含有该聚羟基烷酸酯的调色剂和调色剂，以及使用该调色剂的成像方法和图像形成装置
公开(公告)号：US06777153B2
公开(公告)日：2004-08-17
申请号：US10105305
申请日：2002-03-26
申请人： Tetsuya Yano , Etsuko Sugawa , Takeshi Imamura , Tsutomu Honma , Takashi Kenmoku
发明人： Tetsuya Yano , Etsuko Sugawa , Takeshi Imamura , Tsutomu Honma , Takashi Kenmoku
IPC分类号： G03G900
CPC分类号： C08G63/6882 , C12P7/62 , G03G9/08755 , G03G9/08791 , Y10S435/874 , Y10S435/877
摘要： A polyhydroxyalkanoate characterized by having in the molecule a unit represented by Chemical Formula (1): wherein n may assume any one integral value within the range of from 1 to 8. Also disclosed are a process for producing the polyhydroxyalkanoate by the use of a microorganism having the ability to produce the polyhydroxyalkanoate and accumulate it in the bacterial body; a charge control agent, a toner binder and a toner which contain this polyhydroxyalkanoate; and an image-forming method and an image-forming apparatus which make use of the toner.
摘要翻译：特征在于在分子中具有由化学式（1）表示的单元的聚羟基链烷酸酯：其中n可以在1至8的范围内呈现任何一个整数值。还公开了通过使用微生物制备聚羟基链烷酸酯的方法具有生产聚羟基链烷酸酯并将其积聚在细菌体内的能力; 电荷控制剂，调色剂粘合剂和含有该聚羟基链烷酸酯的调色剂; 以及利用调色剂的图像形成方法和图像形成装置。

5. 发明授权

US06586562B2 Polyhydroxyalkanoate, method for production thereof and microorganisms for use in the same 失效
标题翻译：聚羟基链烷酸酯，其制备方法和用于其的微生物
公开(公告)号：US06586562B2
公开(公告)日：2003-07-01
申请号：US09748205
申请日：2000-12-27
申请人： Tsutomu Honma , Toyoko Kobayashi , Tetsuya Yano , Shin Kobayashi , Takeshi Imamura , Sakae Suda , Takashi Kenmoku
发明人： Tsutomu Honma , Toyoko Kobayashi , Tetsuya Yano , Shin Kobayashi , Takeshi Imamura , Sakae Suda , Takashi Kenmoku
IPC分类号： C08G6306
CPC分类号： C12P7/62 , C08G63/06 , C08G63/6822 , C08G63/6852 , Y10S435/874
摘要： A polyhydroxyalkanoate having a monomer unit composition represented by General Formula (1): AmB(1−m) (1) wherein A is represented by General Formula (2), B is at least one selected from the group consisting of monomer units represented by General Formula (3) or (4), and m has a value of 0.01 or larger and smaller than 1: wherein n has a value of 0 to 10, k has a value of 3 or 5, and R is at least one group selected from the group consisting of groups represented by General Formulae (5) to (7): in Formula (5) R1 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5 and —C3F7; and q is an integer selected from 1 to 8; in Formula (6) R2 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5 and —C3F7; and r is an integer selected from 1 to 8; in Formula (7) R3 is a group selected from the group consisting of a hydrogen atom (H), halogen atoms, —CN, —NO2, —CF3, —C2F5 and —C3F7; and s is an integer selected from 1 to 8. The efficient production methods are also provided.
摘要翻译：具有由通式（1）表示的单体单元组成的聚羟基链烷酸酯：其中A由通式（2）表示，B为选自由通式（3）或（4）表示的单体单元中的至少一种，，m的值为0.01以上且小于1，其中n为0〜10，k为3或5，R为选自由通式（5）〜（7）中，式（5）中，R 1为选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3，-C 2 F 5和-C 3 F 7的基团。 q为选自1至8的整数; 在式（6）中，R 2是选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3，-C 2 F 5和-C 3 F 7的基团; 并且r是选自1至8的整数; 在式（7）中，R 3是选自氢原子（H），卤素原子，-CN，-NO 2，-CF 3，-C 2 F 5和-C 3 F 7的基团; s是选自1至8的整数。还提供了有效的制备方法。

6. 发明申请

US20060211100A1 Novel polyhydroxy alkanoate copolymer including within molecule unit having vinyl group or carboxyl group in side chain, and producing method therefor 有权
标题翻译：在侧链中具有乙烯基或羧基的分子单元内的新型多羟基链烷酸酯共聚物及其制造方法
公开(公告)号：US20060211100A1
公开(公告)日：2006-09-21
申请号：US10531689
申请日：2003-10-23
申请人： Takashi Kenmoku , Tetsuya Yano , Chieko Mihara , Shinya Kozaki , Tsutomu Honma , Tatsuki Fukui , Takeshi Imamura , Etsuko Sugawa
发明人： Takashi Kenmoku , Tetsuya Yano , Chieko Mihara , Shinya Kozaki , Tsutomu Honma , Tatsuki Fukui , Takeshi Imamura , Etsuko Sugawa
IPC分类号： C12P7/62 , C08G63/87
CPC分类号： C08G63/06 , C08G63/6882 , C12P7/625
摘要： The invention provides a PHA copolymer including at least a 3-hydroxy-ω-carboxyalkanoic acid represented by a formula (19) or (32) and simultaneously at least a unit represented by a formula (2) or a formula (3) in a molecule, a precursor PHA copolymer having a corresponding vinyl group or a corresponding alkoxycarbonyl group, a biosynthesis method thereof by microorganisms, and a method of producing a desired PHA copolymer from the precursor PHA copolymer: (wherein k, m, n are integers; R18 represents H, Na, K, R27? represents (A); R1 represents a substituent on a cyclohexyl group and represents H, CN, NO2, a halogen atom, CH3, C2H5, C3H7, CF3, C2F5, or C3F7; R includes a residue including a phenyl structure or a thienyl structure; these being independent for each unit).
摘要翻译：本发明提供一种PHA共聚物，其包含至少一种由式（19）或（32）表示的3-羟基-ω-羧基烷酸，同时至少由式（2）或式（3）表示的单元在分子，具有相应的乙烯基或相应的烷氧基羰基的前体PHA共聚物，微生物的生物合成方法以及由前体PHA共聚物制备所需PHA共聚物的方法（其中k，m，n为整数; R 表示H，Na，K，R 27？表示（A）; R 1表示环己基上的取代基，代表H，CN ，NO 2 2，卤素原子，CH 3，C 2 H 5，C 3， H 3，CF 3，C 2 H 5，或C 3 H 3， R> R包括残基 e包括苯基结构或噻吩基结构; 这些对于每个单元是独立的）。

7. 发明授权

US06872788B2 Production method of polyester containing epoxy group in side chain and production method of crosslinked polymer 失效
标题翻译：侧链中含有环氧基的聚酯的生产方法和交联聚合物的制备方法
公开(公告)号：US06872788B2
公开(公告)日：2005-03-29
申请号：US10427919
申请日：2003-05-02
申请人： Takeshi Imamura , Etsuko Sugawa , Tetsuya Yano , Shin Kobayashi , Tsutomu Honma , Takashi Kenmoku
发明人： Takeshi Imamura , Etsuko Sugawa , Tetsuya Yano , Shin Kobayashi , Tsutomu Honma , Takashi Kenmoku
IPC分类号： C12N1/28 , C12P7/62 , C08F20/00
CPC分类号： C12P7/625 , C12N1/28 , Y10S435/874
摘要： A method is provided which biosynthesizes a PHA having an epoxy group on a side chain with improved physicochemical properties. Specifically, a method of producing a polyester containing an epoxy group in a side chain thereof using alkene as a raw material is provided which comprises the steps of bringing alkene into contact with a microorganism having an ability to uptake alkene and convert it to a polyester and allowing the microorganism to convert the alkene into a polyester containing an epoxy group in a side chain thereof. Further, a method of producing a crosslinked polymer is provided which comprises reacting the polyester obtained by the above mentioned method with a diamine compound.
摘要翻译：提供一种方法，其生物合成具有改善的物理化学性质的侧链上具有环氧基的PHA。具体地说，提供了使用烯烃作为原料在其侧链中制备含有环氧基的聚酯的方法，其包括使烯与具有吸收烯烃的能力并将其转化为聚酯的微生物接触的步骤，以及允许微生物将烯烃转化成其侧链中含有环氧基的聚酯。此外，提供了一种制备交联聚合物的方法，其包括使通过上述方法获得的聚酯与二胺化合物反应。

8. 发明申请

US20050054063A1 Method and apparatus for producing polyhydroxyalkanoate 审中-公开
标题翻译：聚羟基链烷酸酯的制备方法和装置
公开(公告)号：US20050054063A1
公开(公告)日：2005-03-10
申请号：US10940029
申请日：2004-09-14
申请人： Tsutomu Honma , Etsuko Sugawa , Tetsuya Yano , Tsuyoshi Nomoto , Takeshi Imamura , Tomohiro Suzuki , Takashi Kenmoku
发明人： Tsutomu Honma , Etsuko Sugawa , Tetsuya Yano , Tsuyoshi Nomoto , Takeshi Imamura , Tomohiro Suzuki , Takashi Kenmoku
IPC分类号： C08G63/90 , C12M1/00 , C12P7/62 , C12P7/26
CPC分类号： C12P7/625 , C12M47/06
摘要： The present invention provides a method for producing highly purified polyhydroxyalkanoate (PHA) with high yield, by removing cell components other than PHA from cells containing PHA. Moreover, the present invention provides a method for simply removing or reducing chlorine remained in the collected PHA particles, when a treatment with an oxidizing agent containing hypochlorite is performed in the production of PHA as described above. One of the above-described methods comprises a step of treating cells containing polyhydroxyalkanoate with an oxidizing agent containing at least hypochlorite, a step of separating the treated cells into a water-soluble fraction and a water-insoluble fraction, and a step of reducing chlorine remained in the water-insoluble fraction. The step of reducing chlorine may be a step of washing the water-insoluble fraction with a hot water, thiosulfate solution or polar solvent solution containing at least an organic polar solvent in which polyhydroxyalkanoate is insoluble.
摘要翻译：本发明提供了通过从含有PHA的细胞中除去PHA以外的细胞成分而以高产率生产高纯度聚羟基链烷酸酯（PHA）的方法。此外，本发明提供了如上所述在生产PHA中进行含有次氯酸盐的氧化剂的处理时，简单地除去或还原所收集的PHA颗粒中残留的氯的方法。上述方法之一包括用至少含有次氯酸盐的氧化剂处理含有聚羟基链烷酸酯的细胞的步骤，将经处理的细胞分离成水溶性级分和水不溶性级分的步骤以及还原氯保留在水不溶性部分中。还原氯的步骤可以是用至少含有聚羟基链烷酸酯不溶的有机极性溶剂的热水，硫代硫酸盐溶液或极性溶剂溶液洗涤水不溶性部分的步骤。

9. 发明授权

US06861496B2 Polyhydroxyalkonoate and method of producing same, and ω-(2-thienylsulfanyl) alkanoic acid and method of producing same 失效
标题翻译：聚羟基链烷酸酯及其制造方法，ω-（2-噻吩基硫烷基）链烷酸及其制造方法
公开(公告)号：US06861496B2
公开(公告)日：2005-03-01
申请号：US10641090
申请日：2003-08-15
申请人： Takashi Kenmoku , Etsuko Sugawa , Tetsuya Yano , Tsuyoshi Nomoto , Takeshi Imamura , Tomohiro Suzuki , Tsutomu Honma
发明人： Takashi Kenmoku , Etsuko Sugawa , Tetsuya Yano , Tsuyoshi Nomoto , Takeshi Imamura , Tomohiro Suzuki , Tsutomu Honma
IPC分类号： C08G63/60 , C07D333/34 , C08G63/68 , C08G63/688 , C12P7/62 , C12P11/00 , C12R1/38 , C08G63/02
CPC分类号： C12P7/625 , C07D333/34 , C08G63/6882 , C12P11/00 , Y10S435/874 , Y10S435/877
摘要： PHA containing a novel 3-hydroxy-thioalkanoic acid unit having a highly reactive thienyl group in a side chain thereof, and a method of producing the same are provided. Specifically, 5-(2-thienylsulfanyl) valeric acid represented by Chemical Formula [4] below and 6-(2-thienylsulfanyl) hexanoic acid represented by Chemical Formula [5] below are provided. Further, a method of producing PHA, comprising the step of collecting PHA from cells of a microorganism cultured in a medium containing the valeric acid or hexanoic acid, and a novel PHA represented by Chemical Formula [1] below are provided. (n denotes an integer of 1 to 9)
摘要翻译：提供了含有其侧链中具有高反应性噻吩基的新型3-羟基 - 硫代链烷酸单元的PHA及其制备方法。具体地，提供由下述化学式[4]表示的5-（2-噻吩基硫烷基）戊酸和由下述化学式[5]表示的6-（2-噻吩基硫烷基）己酸。此外，提供了一种制备PHA的方法，其包括从含有戊酸或己酸的培养基中培养的微生物的细胞和由下述化学式[1]表示的新型PHA收集PHA的步骤（n表示 1〜9的整数）

10. 发明授权

US06803444B2 Polyhydroxyalkanoate polyester having vinyl phenyl structure in the side chain and its production method 失效
标题翻译：侧链中具有乙烯基苯基结构的聚羟基链烷酸酯聚酯及其制备方法
公开(公告)号：US06803444B2
公开(公告)日：2004-10-12
申请号：US10084167
申请日：2002-02-28
申请人： Tomohiro Suzuki , Etsuko Sugawa , Tetsuya Yano , Tsuyoshi Nomoto , Takeshi Imamura , Tsutomu Honma , Takashi Kenmoku
发明人： Tomohiro Suzuki , Etsuko Sugawa , Tetsuya Yano , Tsuyoshi Nomoto , Takeshi Imamura , Tsutomu Honma , Takashi Kenmoku
IPC分类号： C08G6306
CPC分类号： C12P7/625 , C08G63/06 , Y10S435/874 , Y10S435/877
摘要： Polyhydroxyalkanoate type polyester that comprises one unit % or more of 3-hydroxy-&ohgr;-(4-vinylphenyl)alkanoic acid units. A microbial production method is also provided.
摘要翻译：包含一个单元％以上的3-羟基-ω-（4-乙烯基苯基）链烷酸单元的聚羟基链烷酸酯型聚酯。还提供微生物生产方法。

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