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    • 1. 发明授权
    • Microorganisms capable of producing highly unsaturated fatty acids and process for producing highly unsaturated fatty acids by using the microorganisms
    • 能够生产高度不饱和脂肪酸的微生物和通过使用微生物生产高度不饱和脂肪酸的方法
    • US06582941B1
    • 2003-06-24
    • US08945226
    • 1997-12-29
    • Toshihiro YokochiToro NakaharaTakanori HigashiharaSatohiro TanakaToshiaki Yaguchi
    • Toshihiro YokochiToro NakaharaTakanori HigashiharaSatohiro TanakaToshiaki Yaguchi
    • C12N114
    • C12P7/6427A23K20/158A23K50/75A23K50/80A23L15/00A23L33/12C11B1/00C11C3/00C12P7/6472C12R1/645Y10S435/911Y10S435/946
    • The present invention relates to the Schizochytrium genus SR21 strain and a microorganism belonging to the same species as does said SR21 strain or having substantially the same fungological properties as does said SR21 strain, the said SR21 strain and microorganism having the ability to produce the (n-3) series of docosahexaenoic acid (DHA) and the (n-6) series of docosapentaenoic acid (DPA), and the invention also relates to a process for preparing the (n-3) series of DHA and the (n-6) series of DPA utilizing said microorganisms. The microorganisms according to the present invention are superior in their proliferation character and their propensity to produce fat, and have the ability to produce the (n-3) series of DHA and the (n-6) series of DPA very well. Accordingly, it is possible to effectively produce the (n-3) series of DHA and/or the (n-6) series of DPA, which are useful in the fields of foods and pharmaceuticals, using the microorganisms according to the present invention. In addition, the present invention provides a fat obtained by culturing the present microorganisms. Since the fat composition contains the (n-6) series of DPA in addition to the (n-3) series of DHA having various physiological activities, it is possible to stably and effectively supply the (n-6) series of DPA and/or the (n-3) series of DHA to subjects in need of these highly unsaturated fatty acids by adding the fat composition to various feedstuffs or foods.
    • 本发明涉及裂殖酵菌属SR21菌株和属于与所述SR21菌株相同种类的微生物或具有与所述SR21菌株基本上相同的真菌性质的微生物,所述SR21菌株和微生物具有产生(n -3)系列二十二碳六烯酸(DHA)和(n-6)系列二十二碳五烯酸(DPA),本发明还涉及一种制备(n-3)系列DHA和(n-6) )系列的DPA。 根据本发明的微生物的增殖特性及其产生脂肪的倾向是优越的,并且能够非常好地生产(n-3)系列的DHA和(n-6)系列的DPA。 因此,可以使用本发明的微生物有效地生产可用于食品和药物领域的(n-3)系列的DHA和/或(n-6)系列DPA。 此外,本发明提供通过培养本发明的微生物而获得的脂肪。 由于脂肪组合物除了具有各种生理活性的(n-3)系列的DHA以外还含有(n-6)系DPA,所以可以稳定有效地供给(n-6)系列的DPA和/ 或(n-3)系列DHA通过将脂肪组合物添加到各种饲料或食物中来对需要这些高度不饱和脂肪酸的受试者施用。
    • 2. 发明授权
    • Method of producing a polyunsaturated fatty acid containing culture and polyunsaturated fatty acid containing oil using microorganisms
    • 使用微生物生产含有不饱和脂肪酸的培养物和含有多不饱和脂肪酸的油的方法
    • US06461839B2
    • 2002-10-08
    • US09749035
    • 2000-12-28
    • Toshihiro YokochiToro NakaharaMasakazu YamaokaRyuichiro Kurane
    • Toshihiro YokochiToro NakaharaMasakazu YamaokaRyuichiro Kurane
    • C12P764
    • C12P7/6427A23D9/00C11B1/00C12N1/10C12N1/26C12P7/6472C12R1/90
    • Present invention provides a method to efficiently manufacture an oil-containing product including long chain polyunsaturated fatty acid, or a high added value oil containing long chain polyunsaturated fatty acids. The method comprises culturing a microorganism belonging to the Genus Labyrinthula. The Labyrinthula microorganism is cultured in a medium containing oil or fatty acid as a carbon source, and the produced long chain polyunsaturated fatty acid is recovered from the culture. With the present invention, it is possible to efficiently produce from the vegetable oil and the like, an oil containing product comprising long chain polyunsaturated fatty acids such as docosahexanoic acid having a carbon number of 20 or more, and two or more unsaturated bonds, or an oil containing the long chain polyunsaturated fatty acid. Further, the method of the present invention may be used to reform oil by converting the plant oil and like to high added value oil containing the long chain polyunsaturated fatty acid.
    • 本发明提供了一种有效制造含有长链多不饱和脂肪酸或含有长链多不饱和脂肪酸的高附加值油的含油产品的方法。 该方法包括培养属于Labyrinthula属的微生物。 将Labyrinthula微生物在含有油或脂肪酸作为碳源的培养基中培养,并从培养物中回收生成的长链多不饱和脂肪酸。 通过本发明,可以从植物油等高效地制造含有碳数为20以上的二十二碳六烯酸的长链多不饱和脂肪酸和两个以上的不饱和键的含油产品,或 含有长链多不饱和脂肪酸的油。 此外,本发明的方法可用于通过将植物油转化为含有长链多不饱和脂肪酸的高附加值油来改性油。
    • 3. 发明授权
    • Method for obtaining lipids from fungus bodies
    • 从真菌体获得脂质的方法
    • US4870011A
    • 1989-09-26
    • US905589
    • 1986-08-22
    • Osamu SuzukiToshihiro Yokochi
    • Osamu SuzukiToshihiro Yokochi
    • C11B1/10C11B7/00C12N1/00C12P7/64
    • C12P7/6472C11B1/10C11B7/0008C12N1/005
    • The invention relates to a procedure of obtaining a lipid mixture rich in the content of .gamma.-linolenic acid or glycerides thereof from cultured fungus bodies of a filamentous Mortierella fungus. The invention proposes, on one hand, to fractionally extract the lipid mixture from the fungus bodies under mechanical disintegration in two steps first with an alcohol containing water to give a fraction rich in the polar lipids and then with a hydrocarbon solvent, e.g. hexane, to give a fraction lean in the polar lipids. The invention proposes, on the other hand, to enrich a lipid mixture relative to .gamma.-linolenic acid by fractionally crystallizing a part of a starting lipid mixture in a solution of a specified solvent so that the uncrystallized fraction in the mother liquor contains a lipid mixture in which the content of .gamma.-linolenic acid is remarkably increased in comparison with the starting lipid mixture.
    • PCT No.PCT / JP85 / 00685 Sec。 371日期1986年8月22日第 102(e)日期1986年8月22日PCT提交1985年12月13日PCT公布。 公开号WO86 / 04353 日本1986年7月31日。本发明涉及从丝状高密被孢霉真菌的培养真菌体获得富含γ-亚麻酸或其甘油酯含量的脂质混合物的方法。 本发明一方面提出,首先用含醇的水分两步分离地从机体分解机械分解提取脂质混合物,得到富含极性脂质的馏分,然后用烃溶剂,例如, 己烷,使极性脂质中的馏分变薄。 另一方面,本发明提出了通过将一部分起始脂质混合物在特定溶剂的溶液中分级结晶来富集相对于γ-亚麻酸的脂质混合物,使得母液中的未结晶部分含有脂质混合物 其中与起始脂质混合物相比,γ-亚麻酸的含量显着增加。
    • 5. 发明授权
    • Method for the preparation of a fungal body and a lipid rich in
Y-linolenic acid therefrom
    • 用于制备真菌体和富含γ-亚麻酸的脂质的方法
    • US4783408A
    • 1988-11-08
    • US929601
    • 1986-11-10
    • Osamu SuzukiToshihiro Yokochi
    • Osamu SuzukiToshihiro Yokochi
    • C12N1/14C12P7/64
    • C12P7/6472C12N1/14Y10S435/911
    • A very efficient microbiological method is proposed for the preparation of .gamma.-linolenic acid or a lipid rich in the content of .gamma.-linolenic acid, one of the essential fatty acids in the diet of mammals, e.g. human. The method comprises culturing a fungus of Mortierella genus including isabellina, vinacea, ramanniana, ramanniana var. angulispora and nana in a culture medium containing a carbohydrate, e.g. glucose, as the carbon source in an unusually high concentration of 60 to 400 g/liter to grow a fungal body containing a large amount of a lipid rich in the content of .gamma.-linolenic acid. A further improvement in the efficiency of culture and the content of the desired fatty acid in the fungal body is obtained by the addition of acetic acid or an alkali acetate to the culture medium.
    • 提出了一种非常有效的微生物学方法,用于制备γ-亚麻酸或富含哺乳动物饮食中必需脂肪酸之一的γ-亚麻酸含量的脂质,例如。 人类。 该方法包括培育高山被孢霉属真菌,包括山茶属,藤蔓,苎麻,苎麻属。 (angulispora)和纳那(nana)在含有碳水化合物的培养基中。 葡萄糖作为碳源以非常高的浓度为60〜400g /升,以生长含有大量富含γ-亚麻酸含量的脂质的真菌体。 通过向培养基中加入乙酸或乙酸碱来获得培养效率和真菌体内所需脂肪酸含量的进一步改善。