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1. 发明授权

US06177513B1 Method for evaluating artificial receptors 失效
标题翻译：评估人造受体的方法
公开(公告)号：US06177513B1
公开(公告)日：2001-01-23
申请号：US09266942
申请日：1999-03-12
申请人： Toshifumi Takeuchi , Jun Matsui , Daigo Fukuma , Toshihiro Mizukami
发明人： Toshifumi Takeuchi , Jun Matsui , Daigo Fukuma , Toshihiro Mizukami
IPC分类号： B01D1500
CPC分类号： B01J20/267 , B01D15/00 , B01D15/3852 , B01J19/0046 , B01J20/26 , B01J20/268 , B01J2219/00283 , B01J2219/00306 , B01J2219/00346 , B01J2219/00367 , B01J2219/00373 , B01J2219/00585 , B01J2219/0059 , B01J2219/00702 , B01J2219/00722 , C40B40/14 , C40B60/14 , G01N33/543 , G01N33/54306 , G01N2600/00 , Y10T436/10
摘要： A method for evaluating an artificial receptor includes subjecting a functional monomer and a crosslinker in the presence of a template substance in a vessel into polymerization, thereby forming a resultant polymer with the template substance usually in the form of a thin film, dissociating the template substance from the polymer and removing it from the vessel by washing an inside of the vessel, thereby to prepare an artificial receptor, feeding a solution of the same or different template substance into the vessel to associate the template substance with the resulting polymer; then determining the amount of the template substance remaining in the vessel, and evaluating the artificial receptor on the basis of a determination result.
摘要翻译：用于评价人造受体的方法包括使官能单体和交联剂在容器中的模板物质的存在下进行聚合，由此形成通常为薄膜形式的模板物质的聚合物，使模板物质解离从聚合物中取出并通过洗涤容器的内部将其从容器中除去，从而制备人造受体，将相同或不同的模板物质的溶液进料到容器中以将模板物质与所得聚合物缔合; 然后确定残留在容器中的模板物质的量，并根据确定结果评估人造受体。

2. 发明授权

US08802025B2 Reagent kit for sample analysis and sample analysis method 有权
标题翻译：用于样品分析和样品分析方法的试剂盒
公开(公告)号：US08802025B2
公开(公告)日：2014-08-12
申请号：US12677250
申请日：2008-09-26
申请人： Tatsuya Narikawa , Tomohiro Tsuji , Keiko Moriyama , Yuji Itose , Shinichiro Oguni , Ayumu Yoshida , Saori Suzuki , Toshihiro Mizukami
发明人： Tatsuya Narikawa , Tomohiro Tsuji , Keiko Moriyama , Yuji Itose , Shinichiro Oguni , Ayumu Yoshida , Saori Suzuki , Toshihiro Mizukami
IPC分类号： B01L3/00 , C12Q1/70 , C12Q1/68 , G01N33/53
CPC分类号： G01N33/582 , G01N15/1459 , G01N21/6428 , G01N2015/1006 , G01N2015/1402
摘要： The present invention provides a reagent kit for analyzing a sample comprising a first reagent containing a cationic surfactant, a nonionic surfactant and an aromatic carboxylic acid and a second reagent containing a fluorescent dye capable of staining nucleic acid, and a method for analyzing a sample using the kit.
摘要翻译：本发明提供了一种用于分析样品的试剂盒，其包含含有阳离子表面活性剂，非离子表面活性剂和芳香族羧酸的第一试剂和含有能够染色核酸的荧光染料的第二试剂，以及使用该套件。

3. 发明申请

US20050202400A1 Method of classifying counting leucocytes 有权
标题翻译：白细胞计数分类方法
公开(公告)号：US20050202400A1
公开(公告)日：2005-09-15
申请号：US10517422
申请日：2003-06-23
申请人： Tomohiro Tsuji , Toshihiro Mizukami , Aya Konishi , Yusuke Mori , Yukie Nakazawa
发明人： Tomohiro Tsuji , Toshihiro Mizukami , Aya Konishi , Yusuke Mori , Yukie Nakazawa
IPC分类号： G01N15/00 , G01N15/14 , G01N33/52 , C12Q1/00
CPC分类号： G01N33/52 , G01N2015/1477
摘要： There is provided a method for classifying and counting leukocytes with abnormal DNA amount, which comprises: (1) a step of staining cells in a sample obtained from a hematological sample by treatment with a hemolytic agent to lyse erythrocytes, with a fluorescent dye which can make a difference in the fluorescence intensity at least among mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes; (2) a step of introducing the sample containing the stained cells into a flow cytometer to measure scattered light and fluorescence of the respective cells; (3) a step of classifying leukocytes and coincidence cells/platelet clumps utilizing a difference in the intensity of a scattered light peak and a difference in the scattered light width; (4) a step of classifying and counting mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes, utilizing a difference in the scattered light intensity and a difference in the fluorescence intensity of leukocytes classified in the step (3).
摘要翻译：提供了一种用于对异常DNA量进行白细胞分类和计数的方法，其包括：（1）通过用溶血剂处理从血液样品获得的样品中的细胞染色红细胞裂解红细胞的步骤，可以使用荧光染料至少在成熟白细胞，具有异常DNA量的白细胞和未成熟白细胞之间的荧光强度差异; （2）将含有染色细胞的样品引入流式细胞仪中以测量各细胞的散射光和荧光的步骤; （3）利用散射光峰的强度和散射光宽度的差异来分类白细胞和符合细胞/血小板结块的步骤; （4）利用步骤（3）中分类的散射光强度和白细胞的荧光强度差异，对成熟白细胞，具有异常DNA量的白细胞和未成熟白细胞进行分类和计数的步骤。

4. 发明授权

US08163471B2 Reagent for sample analysis, kit for sample analysis and method for sample analysis 有权
标题翻译：样品分析试剂，样品分析试剂盒和样品分析方法
公开(公告)号：US08163471B2
公开(公告)日：2012-04-24
申请号：US11806904
申请日：2007-06-05
申请人： Toshihiro Mizukami , Hiroki Takeshita , Tatsuya Narikawa
发明人： Toshihiro Mizukami , Hiroki Takeshita , Tatsuya Narikawa
IPC分类号： C12Q1/00
CPC分类号： G01N33/5094
摘要： A reagent for measuring basophils and/or nucleated red blood cells in a sample, which comprises at least one fluorescent dye defined in the formula (I) or (II) is disclosed. Also disclosed are a kit and method for measuring basophils and/or nucleated red blood cells.
摘要翻译：公开了一种用于测量样品中嗜碱性粒细胞和/或有核红细胞的试剂，其包含式（I）或（II）中定义的至少一种荧光染料。还公开了用于测量嗜碱性粒细胞和/或有核红细胞的试剂盒和方法。

5. 发明申请

US20060223138A1 Method and apparatus for counting megakaryocytes 有权
标题翻译：计数巨核细胞的方法和装置
公开(公告)号：US20060223138A1
公开(公告)日：2006-10-05
申请号：US11392651
申请日：2006-03-30
申请人： Yusuke Mori , Toshihiro Mizukami , Tomohiro Tsuji , Yoshiro Ikeuchi
发明人： Yusuke Mori , Toshihiro Mizukami , Tomohiro Tsuji , Yoshiro Ikeuchi
IPC分类号： G01N33/48 , G01N1/30
CPC分类号： G01N15/147 , G01N33/5002 , G01N33/5094 , G01N33/80 , G01N2015/0073
摘要： A method for counting megakaryocytes in a specimen is described. In the method, first, erythrocytes in the specimen are lysed and nucleic acid in the megakaryocytes is stained with a fluorescent dye, and thereby, a measurement sample is prepared. Next, the cells in the measurement sample are irradiated with excited light so that the forward scattered light, the side scattered light and the fluorescence, which are emitted from the cells, are detected. Megakaryocytes are identified on the basis of the detected forward scattered light, the fluorescence and the side scattered light. Then, the identified megakaryocytes are counted.
摘要翻译：描述了一种用于计数样本中的巨核细胞的方法。在该方法中，首先将样品中的红细胞裂解，并用荧光染料染色巨核细胞中的核酸，由此制备测定样品。接下来，用激发光照射测量样品中的单元，从而检测从单元发射的前向散射光，侧向散射光和荧光。在检测到的前向散射光，荧光和侧向散射光的基础上鉴定巨核细胞。然后，计算鉴定的巨核细胞。

6. 发明授权

US08101414B2 Reagent for sample analysis, reagent kit for sample analysis and method for sample analysis 有权
标题翻译：样品分析试剂，样品分析试剂盒和样品分析方法
公开(公告)号：US08101414B2
公开(公告)日：2012-01-24
申请号：US11763827
申请日：2007-06-15
申请人： Toshihiro Mizukami , Hiroki Takeshita , Tatsuya Narikawa
发明人： Toshihiro Mizukami , Hiroki Takeshita , Tatsuya Narikawa
IPC分类号： G01N33/48
CPC分类号： G01N33/5094 , G01N15/1459 , G01N33/56972 , G01N2015/1006 , G01N2015/1402 , Y10T436/101666
摘要： A reagent for measuring a basophil and/or a nucleated red blood cell is described. The reagent comprises (a) an alcohol having 4 to 8 total carbon atoms, and (b) one or more fluorescent dyes selected from the group consisting of a compound of the general formula (I) and a compound of the general formula (II).
摘要翻译：描述了用于测量嗜碱性粒细胞和/或有核红细胞的试剂。试剂包含（a）具有4至8个总碳原子的醇，和（b）选自通式（I）的化合物和通式（II）的化合物的一种或多种荧光染料，。

7. 发明授权

US07625730B2 Method for classifying and counting leukocytes 有权
标题翻译：白细胞分类和计数方法
公开(公告)号：US07625730B2
公开(公告)日：2009-12-01
申请号：US10517422
申请日：2003-06-23
申请人： Tomohiro Tsuji , Toshihiro Mizukami , Aya Konishi , Yusuke Mori , Yukie Nakazawa
发明人： Tomohiro Tsuji , Toshihiro Mizukami , Aya Konishi , Yusuke Mori , Yukie Nakazawa
IPC分类号： C12N13/00
CPC分类号： G01N33/52 , G01N2015/1477
摘要： There is provided a method for classifying and counting leukocytes with abnormal DNA amount, which comprises: (1) a step of staining cells in a sample obtained from a hematological sample by treatment with a hemolytic agent to lyse erythrocytes, with a fluorescent dye which can make a difference in the fluorescence intensity at least among mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes; (2) a step of introducing the sample containing the stained cells into a flow cytometer to measure scattered light and fluorescence of the respective cells; (3) a step of classifying leukocytes and coincidence cells/platelet clumps utilizing a difference in the intensity of a scattered light peak and a difference in the scattered light width; (4) a step of classifying and counting mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes, utilizing a difference in the scattered light intensity and a difference in the fluorescence intensity of leukocytes classified in the step (3).
摘要翻译：提供了一种用于对异常DNA量进行白细胞分类和计数的方法，其包括：（1）通过用溶血剂处理从血液样品获得的样品中的细胞染色红细胞裂解红细胞的步骤，可以使用荧光染料至少在成熟白细胞，具有异常DNA量的白细胞和未成熟白细胞之间的荧光强度差异; （2）将含有染色细胞的样品引入流式细胞仪中以测量各细胞的散射光和荧光的步骤; （3）利用散射光峰的强度和散射光宽度的差异来分类白细胞和符合细胞/血小板结块的步骤; （4）利用步骤（3）中分类的散射光强度和白细胞的荧光强度差异，对成熟白细胞，具有异常DNA量的白细胞和未成熟白细胞进行分类和计数的步骤。

8. 发明授权

US07923229B2 Method of classifying and counting leukocytes 有权
标题翻译：白细胞分类和计数方法
公开(公告)号：US07923229B2
公开(公告)日：2011-04-12
申请号：US11926918
申请日：2007-10-29
申请人： Tomohiro Tsuji , Toshihiro Mizukami , Aya Konishi , Yusuke Mori , Yukie Nakazawa
发明人： Tomohiro Tsuji , Toshihiro Mizukami , Aya Konishi , Yusuke Mori , Yukie Nakazawa
IPC分类号： C12N13/00
CPC分类号： G01N33/52 , G01N2015/1477
摘要： A method for classifying and counting leukocytes, which comprises steps of: staining cells in a sample obtained from a hematological sample by treatment with a hemolytic agent, with a fluorescent dye; introducing the sample containing the stained cells into a flow cytometer to measure first scattered light, second scattered light different from the first scattered light and fluorescence of the respective cells; obtaining scattered light peak intensities and scattered light widths of the respective cells based on the measured first scattered light, obtaining scattered light intensities of the respective cells based on the measured second scattered light, and obtaining fluorescence intensities of the respective cells based on the measured fluorescence light; classifying the cells into a first group and a second group based on the scattered light peak intensities and the scattered light widths, the first group including leukocytes and second group including platelet clumps; classifying the leukocytes included in the first group into at least lymphocytes, monocytes and granulocytes based on the scattered light intensities and the fluorescence intensities; and counting the classified lymphocytes, the classified monocytes and the classified granulocytes.
摘要翻译：一种白细胞分类和计数方法，其特征在于包括以下步骤：用溶血剂用荧光染料染色从血液样品中获得的样品中的细胞; 将含有染色细胞的样品引入流式细胞仪中以测量第一散射光，与第一散射光不同的第二散射光和各细胞的荧光; 基于所测量的第一散射光获得各个单元的散射光峰强度和散射光宽度，基于测量的第二散射光获得各个单元的散射光强度，并且基于测量的荧光获得各个单元的荧光强度光; 基于散射光峰强度和散射光宽度将细胞分类为第一组和第二组，第一组包括白细胞和第二组包括血小板聚集; 基于散射光强度和荧光强度，将第一组中包含的白细胞分类为至少淋巴细胞，单核细胞和粒细胞; 并对分类的淋巴细胞，分类的单核细胞和分类的粒细胞进行计数。

9. 发明申请

US20100330565A1 REAGENT KIT FOR SAMPLE ANALYSIS AND SAMPLE ANALYSIS METHOD 有权
标题翻译：用于样品分析和样品分析方法的试剂盒
公开(公告)号：US20100330565A1
公开(公告)日：2010-12-30
申请号：US12677250
申请日：2008-09-26
申请人： Tatsuya Narikawa , Tomohiro Tsuji , Keiko Moriyama , Yuji Itose , Shinichiro Oguni , Ayumu Yoshida , Saori Suzuki , Toshihiro Mizukami
发明人： Tatsuya Narikawa , Tomohiro Tsuji , Keiko Moriyama , Yuji Itose , Shinichiro Oguni , Ayumu Yoshida , Saori Suzuki , Toshihiro Mizukami
IPC分类号： C12Q1/68
CPC分类号： G01N33/582 , G01N15/1459 , G01N21/6428 , G01N2015/1006 , G01N2015/1402
摘要： The present invention provides a reagent kit for analyzing a sample comprising a first reagent containing a cationic surfactant, a nonionic surfactant and an aromatic carboxylic acid and a second reagent containing a fluorescent dye capable of staining nucleic acid, and a method for analyzing a sample using the kit.
摘要翻译：本发明提供了一种用于分析样品的试剂盒，其包含含有阳离子表面活性剂，非离子表面活性剂和芳香族羧酸的第一试剂和含有能够染色核酸的荧光染料的第二试剂，以及使用该套件。

10. 发明授权

US6004816A Reagent and method for classification and counting of leukocytes 失效
标题翻译：白细胞分类和计数的试剂和方法
公开(公告)号：US6004816A
公开(公告)日：1999-12-21
申请号：US80016
申请日：1998-05-15
申请人： Toshihiro Mizukami , Munetaka Ishiyama , Takashi Sakata
发明人： Toshihiro Mizukami , Munetaka Ishiyama , Takashi Sakata
IPC分类号： G01N33/48 , G01N1/28 , G01N1/30 , G01N15/14 , G01N33/50 , G01N33/52 , G01N33/58 , G01N31/00
CPC分类号： G01N33/52 , G01N33/5094 , Y10S436/80 , Y10T436/10 , Y10T436/101666 , Y10T436/107497
摘要： A reagent for classification and counting of leukocytes, the reagent containing at least one dye which has the following structural formula ##STR1## where R.sub.1 is a hydrogen atom or an alkyl group, R.sub.2 and R.sub.3 each represent a hydrogen atom, a lower alkyl group or a lower alkoxy group, R.sub.4 represents a hydrogen atom, an acyl group or an alkyl group, Z represents a sulfur atom, an oxygen atom, or a carbon atom having a lower alkyl group, n denotes 0, 1 or 2, and X.sup.- represents an anion,and specifically binds to RNA to increase in fluorescence intensity, and a method for classification and counting of leukocytes which uses the reagent, whereby abnormal cells such as immature leukocytes and abnormal leukocytes can be classified and counted easily and highly accurately, and at the same time, classification and counting of normal leukocytes as well as the counting of leukocytes can be performed.
摘要翻译：用于白细胞分类和计数的试剂，所述试剂含有至少一种具有以下结构式的染料，其中R 1是氢原子或烷基，R 2和R 3各自表示氢原子，低级烷基或低级烷氧基基团，R 4表示氢原子，酰基或烷基，Z表示硫原子，氧原子或具有低级烷基的碳原子，n表示0,1或2，X表示阴离子并特异性结合RNA以增加荧光强度，以及使用该试剂的白细胞的分类和计数方法，由此能够容易且高精度地分类和计数诸如未成熟白细胞和异常白细胞的异常细胞，同时可以进行正常白细胞的时间，分类和计数以及白细胞的计数。

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