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    • 1. 发明授权
    • Separation of lymphocytes from lymphocyte-containing suspension by
filtration
    • 通过过滤从淋巴细胞悬浮液中分离淋巴细胞
    • US4246107A
    • 1981-01-20
    • US16600
    • 1979-03-01
    • Yoshinori TakenakaNobuaki TsudaToru Kuroda
    • Yoshinori TakenakaNobuaki TsudaToru Kuroda
    • B01D29/00B01D29/01B01D25/06
    • A61M1/3633A61M2202/0407
    • Lymphocytes are advantageously separated from blood or other lymphocyte-containing suspensions by a method wherein the lymphocyte-containing suspension is flowed through a second filter having a mass of fibers exhibiting an average fiber diameter of greater than 10 microns but not greater than 60 microns, thereby to entrap a substantial part of granulocytes and monocytes in the mass of fibers and to obtain a granulocyte-poor and monocyte-poor lymphocyte-containing suspension; and then, the obtained suspension is flowed through a first filter having a mass of fibers exhibiting a bulk density of 0.04 to 0.40 g/cm.sup.3 and an average fiber diameter of 5 to 20 microns, said average fiber diameter being less than that of the second filter, thereby to entrap a substantial part of lymphocytes in the mass of fibers; and finally, the lymphocytes entrapped in the mass of fibers of the first filter is collected.Alternatively, another method is employed wherein the lymphocyte-containing suspension is flowed through the first filter thereby to entrap a substantial part of leukocyte components therein; and the leukocyte components entrapped in the first filter is collected to obtain a leukocyte component-rich suspension; and finally, the leukocyte component-rich suspension is flowed through the second filter thereby to entrap a substantial part of granulocytes and monocytes in the second filter and to obtain a lymphocyte-rich suspension.
    • 淋巴细胞有利地通过其中含淋巴细胞的悬浮液流过具有大于10微米但不大于60微米的平均纤维直径的纤维质量的第二过滤器的方法与血液或其它含淋巴细胞的悬浮液分离,由此 在大量纤维中捕获大部分粒细胞和单核细胞,并获得含有粒细胞的贫血和不含单核细胞的淋巴细胞悬浮液; 然后将获得的悬浮液流过具有堆积密度为0.04-0.40g / cm3和平均纤维直径为5至20微米的纤维质量的第一过滤器,所述平均纤维直径小于第二过滤器的平均纤维直径 过滤器,从而将大部分淋巴细胞捕获在纤维团中; 并且最后收集被捕获在第一过滤器的纤维质量中的淋巴细胞。 或者,使用另一种方法,其中含淋巴细胞的悬浮液流过第一过滤器,从而在其中截留大部分白细胞成分; 收集包含在第一过滤器中的白细胞成分以获得富含白细胞成分的悬浮液; 最后,将含白细胞成分的悬浮液流过第二过滤器,从而将大部分粒细胞和单核细胞捕获到第二过滤器中并获得富含淋巴细胞的悬浮液。
    • 9. 发明授权
    • Absorbent of autoantibody and immune complexes, adsorbing device and
blood purifying apparatus comprising the same
    • 自身抗体和免疫复合物的吸收剂,包含其的吸附装置和血液净化装置
    • US4627915A
    • 1986-12-09
    • US592631
    • 1984-03-23
    • Toru KurodaNaokuni Yamawaki
    • Toru KurodaNaokuni Yamawaki
    • A61M1/36B01D15/08B01J20/28B01J20/32B01D15/00
    • A61M1/3679B01D15/08B01D15/3804B01J20/321B01J20/3219B01J20/3246B01J20/3248B01J20/3251B01J20/3285
    • An adsorbent for adsorbing thereonto an autoantibody and/or immune complexes from a body fluid. The adsorbent comprises a surface, a hydrophobic member and a negative charge-producing member. The negative charge-producing member is adapted to produce such an effective number of negative charges in a body fluid that the ratio of: ##EQU1## is greater than 1. The adsorbent is excellent in selectively and efficiently adsorbing an autoantibody and/or immune complexes while little adsorbing useful substances from the body fluid. The adsorbent may be used in an adsorbing device comprising a vessel having a fluid inlet and a fluid outlet with the adsorbent contained in the vessel. The adsorbing device may be used in a blood purifying apparatus for adsorbing and removing an autoantibody and/or immune complexes from blood plasma, which apparatus comprises a blood circulation passage provided with a plasma separating means and a blood-plasma mixing means, and a plasma recycle passage connected to the blood circulation passage and adapted to introduce plasma, separated by the plasma separating means and purified by a plasma purifying means comprised of the above-mentioned adsorbing device and disposed in the plasma recycle passage, into the mixing device.
    • 一种吸附剂,用于在体液上吸附其自身抗体和/或免疫复合物。 吸附剂包括表面,疏水性构件和负电荷产生构件。 负电荷产生构件适于在体积流体中产生如此有效数量的负电荷,其比例大于1.吸附剂选择性且有效地吸附自身抗体和/或免疫复合物 同时从体液吸附有用物质少。 吸附剂可用于吸附装置,该吸附装置包括具有流体入口的容器和具有容纳在容器中的吸附剂的流体出口。 吸附装置可以用于血液净化装置,用于从血浆中吸附和去除自身抗体和/或免疫复合物,该装置包括设置有血浆分离装置和血浆混合装置的血液循环通道,以及血浆 循环通道连接到血液循环通道并适于引入等离子体,由等离子体分离装置分离,并通过由上述吸附装置构成的等离子体净化装置进行净化,并设置在等离子体循环通道中,进入混合装置。