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1. 发明申请

US20100261259A1 Expression of Genes from Gram Negative Bacteria in Fungi 审中-公开
标题翻译：革兰氏阴性菌在真菌中的表达
公开(公告)号：US20100261259A1
公开(公告)日：2010-10-14
申请号：US12376372
申请日：2007-08-03
申请人： Tomoko Matsui , Pia Francke Johannesen , Shinobu Takagi , Thomas Agersten Poulsen , Noriko Tsutsumi , Zheng Liu , XiangYu Kong
发明人： Tomoko Matsui , Pia Francke Johannesen , Shinobu Takagi , Thomas Agersten Poulsen , Noriko Tsutsumi , Zheng Liu , XiangYu Kong
IPC分类号： C12N1/19 , C12N1/15 , C07H21/04
CPC分类号： C12N9/16 , C07K2319/02 , C12N15/80 , C12P21/02
摘要： The present invention provides a method for the recombinant expression of polypeptides originating from gram negative bacteria, in a fungal host suitable for industrial production. In a first aspect the present invention relates to a method for recombinant expression of a polypeptide from a gram negative bacterium in a fungal host cell, comprising the steps: i) providing a nucleic acid sequence encoding the polypeptide, said nucleic acid sequence comprising a first nucleic acid sequence encoding a fungal signal peptide and a second nucleic acid sequence encoding the polypeptide, having at least one modified codon, wherein the modification does not change the amino acid encoded by said codon and the nucleic acid sequence of said codon is different compared to the corresponding codon in the wild type nucleic acid sequence present in the said gram negative bacterium; ii) expressing the modified nucleic acid sequence in the fungal host.
摘要翻译：本发明提供了在适于工业生产的真菌宿主中重组来自革兰氏阴性细菌的多肽的方法。在第一方面，本发明涉及在真菌宿主细胞中从革兰氏阴性细菌重组表达多肽的方法，其包括以下步骤：i）提供编码所述多肽的核酸序列，所述核酸序列包含第一编码真菌信号肽的核酸序列和编码具有至少一个修饰密码子的多肽的第二核酸序列，其中所述修饰不改变由所述密码子编码的氨基酸，并且所述密码子的核酸序列与存在于所述革兰氏阴性细菌中的野生型核酸序列中相应的密码子; ii）在真菌宿主中表达经修饰的核酸序列。

2. 发明申请

US20120003700A1 Thermostable Alpha-Amylases 审中-公开
标题翻译：热稳定性α-淀粉酶
公开(公告)号：US20120003700A1
公开(公告)日：2012-01-05
申请号：US13230984
申请日：2011-09-13
申请人： Tang Lan , Wu Wenping , Junxin Duan , Pia Francke Johannesen
发明人： Tang Lan , Wu Wenping , Junxin Duan , Pia Francke Johannesen
IPC分类号： C12P19/16 , C12N15/63 , C12N1/15 , C12P7/02 , C12N5/10 , C12N1/21 , C12N15/56 , C12N9/26
CPC分类号： C12N9/242 , A21D8/042 , C12C11/003 , C12N9/2414 , C12P7/02 , C12P19/12 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
摘要： The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO: 4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO: 3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.
摘要翻译：本发明涉及一种分离的多核苷酸，其包含编码具有α-淀粉酶活性的多肽的开放阅读框，所述多肽选自：a）包含与氨基酸具有至少70％同一性的氨基酸序列的多肽 SEQ ID NO：4的22至450; b）一种多肽，其包含氨基酸序列，该氨基酸序列与插入存在于根据布达佩斯条约与DSMZ保藏的大肠杆菌宿主的质粒存在的质粒的多核苷酸编码的多肽编码的多肽相同， 15334; c）由多核苷酸编码的多肽，其包含与SEQ ID NO：3中的位置68至1417所示的序列具有至少70％同一性的核苷酸序列; 和d）具有α-淀粉酶活性的（a），（b）或（c）的片段。

3. 发明申请

US20100209996A1 Thermostable Alpha-Amylases 有权
标题翻译：热稳定性α-淀粉酶
公开(公告)号：US20100209996A1
公开(公告)日：2010-08-19
申请号：US12755076
申请日：2010-04-06
申请人： Lan Tang , Wenping Wu , Junxin Duan , Pia Francke Johannesen
发明人： Lan Tang , Wenping Wu , Junxin Duan , Pia Francke Johannesen
IPC分类号： C12N9/30
CPC分类号： C12N9/242 , A21D8/042 , C12C11/003 , C12N9/2414 , C12P7/02 , C12P19/12 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
摘要： The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of:a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO: 4;b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334;c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO: 3; andd) a fragment of (a), (b) or (c) that has alpha-amylase activity.
摘要翻译：本发明涉及一种分离的多核苷酸，其包含编码具有α-淀粉酶活性的多肽的开放阅读框，所述多肽选自：a）包含与氨基酸具有至少70％同一性的氨基酸序列的多肽 SEQ ID NO：4的22至450; b）一种多肽，其包含氨基酸序列，该氨基酸序列与插入存在于根据布达佩斯条约与DSMZ保藏的大肠杆菌宿主的质粒存在的质粒的多核苷酸编码的多肽编码的多肽相同， 15334; c）由多核苷酸编码的多肽，其包含与SEQ ID NO：3中的位置68至1417所示的序列具有至少70％同一性的核苷酸序列; 和d）具有α-淀粉酶活性的（a），（b）或（c）的片段。

4. 发明授权

US06326184B1 Method of producing a composite fermented beverage using genetically modified yeast strains 失效
标题翻译：使用遗传修饰酵母菌株生产复合发酵饮料的方法
公开(公告)号：US06326184B1
公开(公告)日：2001-12-04
申请号：US09153310
申请日：1998-09-15
申请人： Claes Gjermansen , Jorgen Hansen , Pia Francke Johannesen , Mogens Bohl Pedersen , Steen Bech Sorensen
发明人： Claes Gjermansen , Jorgen Hansen , Pia Francke Johannesen , Mogens Bohl Pedersen , Steen Bech Sorensen
IPC分类号： C12N116
CPC分类号： C12C12/00 , C12C5/026 , C12C5/04 , C12C11/09 , C12C11/11 , C12C12/004 , C12R1/86 , C12R1/865
摘要： Method of preparing a composite yeast fermented beverage such as beer including lager, with predetermined content of flavour compounds, comprising combining separate batches of beverage, of which at least one is a base beverage produced with a yeast strain having reduced or lacking production of one or more flavour compounds or flavour stabilizing compounds. In the method are used yeast strains including S. cerevisiae and S. carlsbergensis which have reduced or lacking production of sulphite, dimethylsulphide, thiols, thioesters, hydrogen sulphide, higher alcohols including isoamyl alcohol and/or alcohol esters.
摘要翻译：制备复合酵母发酵饮料如啤酒的方法，包括含有预定含量的风味化合物的啤酒，其包括组合单独的批次饮料，其中至少一种是由具有减少或不产生一种或多种更多的风味化合物或风味稳定化合物。在该方法中使用酵母菌株，包括酿酒酵母（S.cerevisiae）和卡尔斯氏菌（S.carlsbergensis），其具有减少或缺乏产生亚硫酸盐，二甲基硫化物，硫醇，硫酯，硫化氢，包括异戊醇和/或醇酯的高级醇。

5. 发明授权

US08039241B2 Thermostable alpha-amylases 有权
标题翻译：热稳定性α-淀粉酶
公开(公告)号：US08039241B2
公开(公告)日：2011-10-18
申请号：US12755076
申请日：2010-04-06
申请人： Lan Tang , Wenping Wu , Junxin Duan , Pia Francke Johannesen
发明人： Lan Tang , Wenping Wu , Junxin Duan , Pia Francke Johannesen
IPC分类号： C12N9/30 , C12N9/28 , C12N1/20 , C12N15/00 , C12P19/14 , C12P21/06 , C12P19/34 , D06M16/00 , C07H21/04 , A21D2/00
CPC分类号： C12N9/242 , A21D8/042 , C12C11/003 , C12N9/2414 , C12P7/02 , C12P19/12 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
摘要： The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 1 to 450 of SEQ ID NO: 4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO: 3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.
摘要翻译：本发明涉及一种分离的多核苷酸，其包含编码具有α-淀粉酶活性的多肽的开放阅读框，所述多肽选自：a）包含与氨基酸具有至少70％同一性的氨基酸序列的多肽 1至450个SEQ ID NO：4; b）一种多肽，其包含氨基酸序列，该氨基酸序列与插入存在于根据布达佩斯条约与DSMZ保藏的大肠杆菌宿主的质粒存在的质粒的多核苷酸编码的多肽编码的多肽相同， 15334; c）由多核苷酸编码的多肽，其包含与SEQ ID NO：3中的位置68至1417所示的序列具有至少70％同一性的核苷酸序列; 和d）具有α-淀粉酶活性的（a），（b）或（c）的片段。

6. 发明授权

US07189552B2 Thermostable alpha-amylases 有权
标题翻译：热稳定性α-淀粉酶
公开(公告)号：US07189552B2
公开(公告)日：2007-03-13
申请号：US10539396
申请日：2003-12-16
申请人： Tang Lan , Wu Wenping , Junxin Duan , Pia Francke Johannesen
发明人： Tang Lan , Wu Wenping , Junxin Duan , Pia Francke Johannesen
IPC分类号： C12P7/06 , C12P19/36 , C12P21/06 , C12N9/32 , C12N15/63 , C07H21/04 , D06M16/00
CPC分类号： C12N9/242 , A21D8/042 , C12C11/003 , C12N9/2414 , C12P7/02 , C12P19/12 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
摘要： An isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO:4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO:3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.
摘要翻译：一种分离的多核苷酸，其包含编码具有α-淀粉酶活性的多肽的开放阅读框，所述多肽选自：a）包含氨基酸序列的多肽，所述氨基酸序列与SEQ ID NO：22至450的氨基酸具有至少70％的同一性编号：4; b）一种多肽，其包含氨基酸序列，该氨基酸序列与插入存在于根据布达佩斯条约与DSMZ保藏的大肠杆菌宿主的质粒存在的质粒的多核苷酸编码的多肽编码的多肽相同， 15334; c）由多核苷酸编码的多肽，其包含与SEQ ID NO：3中的位置68至1417所示的序列具有至少70％同一性的核苷酸序列; 和d）具有α-淀粉酶活性的（a），（b）或（c）的片段。

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