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1. 发明授权

US09534239B2 Glucoamylase variants and polynucleotides encoding same 有权
标题翻译：葡糖淀粉酶变体和编码相同的多核苷酸
公开(公告)号：US09534239B2
公开(公告)日：2017-01-03
申请号：US14343278
申请日：2012-09-05
申请人： Tomoko Matsui , Suzanne Clark
发明人： Tomoko Matsui , Suzanne Clark
IPC分类号： C12P7/14 , C12P19/14 , C12N9/34 , C12P19/02 , C13K1/06 , C12C5/00
CPC分类号： C12P7/14 , C12C5/004 , C12N9/2428 , C12P19/02 , C12P19/14 , C12Y302/01003 , C13K1/06 , Y02P20/52
摘要： The present invention relates to glucoamylase variants having reduced sensitivity to protease nicking. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
摘要翻译：本发明涉及对蛋白酶切口具有降低的敏感性的葡糖淀粉酶变体。本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。

2. 发明申请

US20140017749A1 Processes for Producing Fermentation Products 有权
标题翻译：生产发酵产品的工艺
公开(公告)号：US20140017749A1
公开(公告)日：2014-01-16
申请号：US13994310
申请日：2011-12-21
申请人： Randy Deinhammer , Suzanne Clark , Mauricio Quiros , John Matthews , Anne Glud Hjulmand , Chee Leong Soong , Tomoko Matsui , Shinobu Takagi
发明人： Randy Deinhammer , Suzanne Clark , Mauricio Quiros , John Matthews , Anne Glud Hjulmand , Chee Leong Soong , Tomoko Matsui , Shinobu Takagi
IPC分类号： C12P7/06
CPC分类号： C12P7/06 , C12P19/02 , C12P19/14 , C12P19/20 , C12Y302/01001 , C12Y302/01003 , C12Y304/24 , Y02E50/17
摘要： The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable alpha-amylase and optionally a thermostable protease are present and/or added during liquefaction. The invention also relates to a composition suitable for use in a process of the invention.
摘要翻译：本发明涉及用于从含淀粉材料生产发酵产物的方法，其中在液化期间存在和/或加入热稳定性α-淀粉酶和任选的热稳定蛋白酶。本发明还涉及适用于本发明方法的组合物。

3. 发明申请

US20120330493A1 METHOD AND APPARATUS FOR DETERMINING ROAD SURFACE CONDITION 审中-公开
标题翻译：用于确定道路表面条件的方法和装置
公开(公告)号：US20120330493A1
公开(公告)日：2012-12-27
申请号：US13527917
申请日：2012-06-20
申请人： Yasushi HANATSUKA , Tomoyuki HIGUCHI , Tomoko MATSUI
发明人： Yasushi HANATSUKA , Tomoyuki HIGUCHI , Tomoko MATSUI
IPC分类号： G06F17/16
CPC分类号： B60T8/172 , B60T2270/86
摘要： A method, featuring robustness against changes in tire size, is provided for determining a road surface condition by dividing a time-series waveform of tire vibrations into windows without resorting to detection of the peak positions or measurement of the wheel speed. A time-series waveform of tire vibrations detected by a tire vibration detecting unit is windowed by a windowing unit. Time-series waveforms are extracted from the respective time windows, feature vectors X are calculated therefor, and then likelihoods Z for road-surface HMMs (hidden Markov models) are calculated. The likelihoods Z1 to Z5 calculated for the respective road-surface HMMs are compared with one another, and a road surface condition corresponding to the road-surface HMM showing the highest likelihood is determined to be the condition of the road surface on which the tire is running.
摘要翻译：通过将轮胎振动的时间序列波形划分为窗口来确定路面状态，而不需要检测峰值位置或车轮速度的测量，提供了一种具有抗轮胎尺寸变化的鲁棒性的方法。由轮胎振动检测单元检测到的轮胎振动的时间序列波形由加窗单元加窗。从相应的时间窗口提取时间序列波形，计算特征向量X，然后计算路面HMM（隐马尔可夫模型）的可能性Z。对各路面HMM计算出的可能性Z1〜Z5进行比较，将与表现出最高似然度的路面HMM对应的路面状态确定为轮胎为路面的路面状况跑步

4. 发明申请

US20120225468A1 Hafnia Phytase Variants 有权
标题翻译： Hafnia植酸酶变体
公开(公告)号：US20120225468A1
公开(公告)日：2012-09-06
申请号：US13472822
申请日：2012-05-16
申请人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
发明人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
IPC分类号： C12N9/16
CPC分类号： C12N9/16 , A23K20/189 , A23K50/30 , A23K50/75 , Y02E50/17
摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.
摘要翻译：本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度简介和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

5. 发明授权

US08206962B2 Hafnia phytase variants 有权
标题翻译： Hafnia植酸酶变体
公开(公告)号：US08206962B2
公开(公告)日：2012-06-26
申请号：US12568312
申请日：2009-09-28
申请人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
发明人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
IPC分类号： C12N9/14 , C12Q1/34 , C07H21/04
CPC分类号： C12N9/16 , A23K20/189 , A23K50/30 , A23K50/75 , Y02E50/17
摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.
摘要翻译：本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度简介和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

6. 发明授权

US08124379B2 Signal peptide for producing a polypeptide 有权
标题翻译：产生多肽的信号肽
公开(公告)号：US08124379B2
公开(公告)日：2012-02-28
申请号：US12415174
申请日：2009-03-31
申请人： Tomoko Matsui , Henriette Draborg , Steffen Danielsen
发明人： Tomoko Matsui , Henriette Draborg , Steffen Danielsen
IPC分类号： C12N15/62 , C12N15/80 , C12N15/81
CPC分类号： C12N9/6472 , C07K14/37 , C12N9/16 , C12N9/2437 , C12N9/6424 , C12N15/625
摘要： The present invention relates to a method for producing a polypeptide comprising using a signal peptide, to nucleic acid constructs comprising a first nucleotide sequence encoding the signal peptide and a second nucleotide sequence encoding a polypeptide which is foreign to the first nucleotide sequence. Furthermore, it also relates to expression vectors and host cells comprising the nuclei acid construct.
摘要翻译：本发明涉及包含使用信号肽的多肽的制备方法，其包含编码信号肽的第一核苷酸序列的核酸构建体和编码第一核苷酸序列异源的多肽的第二核苷酸序列。此外，它还涉及包含核酸构建体的表达载体和宿主细胞。

7. 发明授权

US07883883B2 Enzymes for starch processing 有权
标题翻译：淀粉加工酶
公开(公告)号：US07883883B2
公开(公告)日：2011-02-08
申请号：US10877849
申请日：2004-06-25
申请人： Hiroaki Udagawa , Rikako Taira , Shinobu Takagi , Carsten Hjort , Anders Vikso-Nielsen , Eric Allain , Shiro Fukuyama , Tomoko Matsui
发明人： Hiroaki Udagawa , Rikako Taira , Shinobu Takagi , Carsten Hjort , Anders Vikso-Nielsen , Eric Allain , Shiro Fukuyama , Tomoko Matsui
IPC分类号： C12N9/24 , C12N9/30 , C07H21/04
CPC分类号： C12P19/14 , C07K2319/00 , C12N9/2408 , C12N9/2411 , C12N9/2414 , C12N9/242 , C12N9/2425 , C12N9/2428 , C12P7/06 , C12P19/02 , C12P19/16 , C12P19/22 , C13K11/00 , Y02E50/17
摘要： The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction.
摘要翻译：本发明涉及包含碳水化合物结合模块氨基酸序列和真菌α-淀粉酶氨基酸序列以及包含碳水化合物结合模块和α-淀粉酶催化模块的真菌野生型酶的变体的杂合酶。本发明还涉及混合酶或变体在淀粉液化中的用途。

8. 发明申请

US20100221385A1 ASPARAGINASES 有权
公开(公告)号：US20100221385A1
公开(公告)日：2010-09-02
申请号：US12782150
申请日：2010-05-18
申请人： TOMOKO MATSUI , ESBEN PETER FRIIS , AKIHIKO YAMAGISHI
发明人： TOMOKO MATSUI , ESBEN PETER FRIIS , AKIHIKO YAMAGISHI
IPC分类号： A23L1/105 , C12N9/82 , C07H21/04 , C12N15/63 , C12N1/15 , C12P21/06 , A23L1/212
CPC分类号： C12N9/82 , A23L5/25 , A23L19/18 , C12Y305/01001
摘要： The invention relates to new asparaginases having improved properties, preferably improved thermotolerance, such as improved activity at high temperatures and/or improved thermostability. The invention also relates to DNA sequences encoding such improved asparaginases, their production in a recombinant host cell, as well as methods of using the asparaginases, in particular for reduction of acrylamide in foods. The invention furthermore relates to methods of generating and preparing asparaginase variants having improved properties.

9. 发明申请

US20100180095A1 BUFFER CONTROL DEVICE AND BUFFER MEMORY DEVICE 审中-公开
标题翻译：缓冲器控制器件和缓冲器存储器件
公开(公告)号：US20100180095A1
公开(公告)日：2010-07-15
申请号：US12095610
申请日：2006-11-28
申请人： Masanori Fujibayashi , Nobuo Higaki , Kazushi Kurata , Tomoko Matsui
发明人： Masanori Fujibayashi , Nobuo Higaki , Kazushi Kurata , Tomoko Matsui
IPC分类号： G06F12/14
CPC分类号： G06F5/14 , G06F2205/062
摘要： The buffer control device of this invention includes: a pointer holding unit which holds a virtual pointer different from a read pointer and a write pointer; an access control unit that controls an access to a ring buffer; a judging unit that judges whether or not one of the read pointer and the write pointer has reached an address substantially identical to an address indicated by the virtual pointer; and disabling unit that disables a normal access using the one of the read pointer and the write pointer, when the judging unit judges that the one of the read pointer and the write pointer has reached the address substantially identical to the address indicated by the virtual pointer, the normal access being controlled by the access control unit, wherein the access control unit further controls a reaccess to the ring buffer.
摘要翻译：本发明的缓冲器控制装置包括：保持与读指针不同的虚拟指针和写指针的指针保持单元; 访问控制单元，其控制对环形缓冲器的访问; 判断单元，判断读指针和写指针之一是否达到与虚拟指针所指示的地址基本相同的地址; 以及禁止单元，其在所述读取指针和所述写入指针之一达到与所述虚拟指针所指示的地址基本相同的地址的情况下，使用所述读取指针和所述写入指针中的一者禁止正常访问正常访问由访问控制单元控制，其中访问控制单元进一步控制对环形缓冲器的重新访问。

10. 发明授权

US07666652B2 Asparaginases 有权
标题翻译：天冬酰胺酶
公开(公告)号：US07666652B2
公开(公告)日：2010-02-23
申请号：US12044055
申请日：2008-03-07
申请人： Tomoko Matsui , Esben Peter Friis , Akihiko Yamagishi
发明人： Tomoko Matsui , Esben Peter Friis , Akihiko Yamagishi
IPC分类号： C12N9/82 , C07H21/04 , C12N1/20 , A21D2/00
CPC分类号： C12N9/82 , A23L5/25 , A23L19/18 , C12Y305/01001
摘要： The invention relates to new asparaginases having improved properties, preferably improved thermotolerance, such as improved activity at high temperatures and/or improved thermostability. The invention also relates to DNA sequences encoding such improved asparaginases, their production in a recombinant host cell, as well as methods of using the asparaginases, in particular for reduction of acrylamide in foods. The invention furthermore relates to methods of generating and preparing asparaginase variants having improved properties.
摘要翻译：本发明涉及具有改进性能的新型天冬酰胺酶，优选改进的耐热性，例如在高温下改善的活性和/或改善的热稳定性。本发明还涉及编码这种改良天冬酰胺酶的DNA序列，其在重组宿主细胞中的产生，以及使用天冬酰胺酶，特别是用于还原食品中丙烯酰胺的方法。本发明还涉及产生和制备具有改进性质的天冬酰胺酶变体的方法。

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