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1. 发明申请

US20120015380A1 ANTI-POLYETHYLENE GLYCOL ANTIBODY EXPRESSING CELL QUANTIFY ANY FREE POLYETHYLENE GLYCOL AND POLYETHYLENE GLYCOL-DERIVATIZED MOLECULES 审中-公开
标题翻译：抗聚乙二醇抗体表达细胞定量任意的聚乙烯甘油和聚乙二醇衍生的分子
公开(公告)号：US20120015380A1
公开(公告)日：2012-01-19
申请号：US13032317
申请日：2011-02-22
申请人： Tian-Lu Cheng , Steven R. Roffler , Kuo-Hsiang Chuang , Ssu-Jung Lu
发明人： Tian-Lu Cheng , Steven R. Roffler , Kuo-Hsiang Chuang , Ssu-Jung Lu
IPC分类号： G01N33/98 , C12N5/071
CPC分类号： G01N33/94 , C07K16/44 , C12N2503/02 , G01N33/5308 , G01N33/9493
摘要： In this invention, anti-PEG antibodies or anti-methoxyl-PEG (anti-CH3O-PEG) antibodies were expressed on cell surface which can collocate with a biotinylated anti-PEG antibody (AGP4-Biotin) or biotinylated PEG (PEG-Biotin) to develop a cell-based sandwich ELISA or a cell-based competition ELISA, respectively. Both of these two methods could sensitively quantify free PEG and PEG-modified macromolecules (proteins, nanoparticles and liposomes) as sensitive as nano-gram level.
摘要翻译：在本发明中，在可以与生物素化的抗-PEG抗体（AGP4-生物素）或生物素化PEG（PEG-生物素））配位的细胞表面上表达抗-PEG抗体或抗甲氧基-PEG（抗-CH 3 O-PEG）分别开发基于细胞的夹心ELISA或基于细胞的竞争ELISA。这两种方法都可以敏感地将游离的PEG和PEG修饰的大分子（蛋白质，纳米颗粒和脂质体）定量为纳克级。

2. 发明授权

US08507207B2 Recombinant nucleotide sequence, cell or vector containing the same and method for using cell containing the same to encode anti-polyethylene glycol monoclonal antibodies 有权
标题翻译：重组核苷酸序列，含有该重组核苷酸序列的细胞或载体以及使用含有该重组核苷酸序列的细胞编码抗聚乙二醇单克隆抗体的方法
公开(公告)号：US08507207B2
公开(公告)日：2013-08-13
申请号：US12766930
申请日：2010-04-26
申请人： Tian-Lu Cheng , Steve R. Roffler , Kuo-Hsiang Chuang
发明人： Tian-Lu Cheng , Steve R. Roffler , Kuo-Hsiang Chuang
IPC分类号： G01N33/53 , C12Q1/68 , C12N15/64 , C12N15/00 , C07H21/04
CPC分类号： A61K49/0054 , A61K49/0019 , A61K49/0032 , A61K49/186 , A61K51/065 , B82Y5/00
摘要： The invention provides a recombinant nucleotide sequence, including the sequence of SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 or SEQ ID No. 4, wherein the recombinant nucleotide sequence encodes an anti-polyethylene glycol recombinant single chain membrane antibody.
摘要翻译：本发明提供重组核苷酸序列，其包括SEQ ID No.1，SEQ ID No.2，SEQ ID No.3或SEQ ID No.4的序列，其中重组核苷酸序列编码抗聚乙二醇重组单链膜抗体。

3. 发明申请

US20110064651A1 RECOMBINANT NUCLEOTIDE SEQUENCE, CELL OR VECTOR CONTAINING THE SAME AND METHOD FOR USING CELL CONTAINING THE SAME TO ENCODE ANTI-POLYETHYLENE GLYCOL MONOCLONAL ANTIBODIES 有权
标题翻译：重组核酸序列，含有该重组核苷酸序列的细胞或载体以及使用含有其的细胞编码抗聚乙二醇单克隆抗体的方法
公开(公告)号：US20110064651A1
公开(公告)日：2011-03-17
申请号：US12766930
申请日：2010-04-26
申请人： Tian-Lu Cheng , Steve R. Roffler , Kuo-Hsiang Chuang
发明人： Tian-Lu Cheng , Steve R. Roffler , Kuo-Hsiang Chuang
IPC分类号： A61K51/00 , C07H21/04 , C12N15/63 , C12N5/00 , C12P21/00 , A61K49/00 , A61B10/00
CPC分类号： A61K49/0054 , A61K49/0019 , A61K49/0032 , A61K49/186 , A61K51/065 , B82Y5/00
摘要： The invention provides a recombinant nucleotide sequence, including the sequence of SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 or SEQ ID No. 4, wherein the recombinant nucleotide sequence encodes an anti-polyethylene glycol recombinant single chain membrane antibody.
摘要翻译：本发明提供重组核苷酸序列，其包括SEQ ID No.1，SEQ ID No.2，SEQ ID No.3或SEQ ID No.4的序列，其中重组核苷酸序列编码抗聚乙二醇重组单链膜抗体。

4. 发明授权

US07320791B2 Monoclonal antibody for analysis and clearance of polyethylene glycol and polyethylene glycol-modified molecules 有权
标题翻译：用于分析和清除聚乙二醇和聚乙二醇改性分子的单克隆抗体
公开(公告)号：US07320791B2
公开(公告)日：2008-01-22
申请号：US10620091
申请日：2003-07-15
申请人： Steve Roffler , Tian-Lu Cheng , Pin-Yi Wu
发明人： Steve Roffler , Tian-Lu Cheng , Pin-Yi Wu
IPC分类号： A61K39/00
CPC分类号： G01N33/5308 , A61K2039/505 , C07K16/44 , G01N2430/60
摘要： A novel anti-polyethylene glycol monoclonal antibody and its preparation are disclosed. Such an antibody can be used for determining polyethylene glycol concentration in vitro or accelerating the clearance of a polyethylene glycol containing compound from the blood circulation in the human body thereby reducing the toxicity associated with the polyethylene glycol containing conjugate. The antibody is particularly useful in cancer therapy where the therapeutic agent is selectively delivered to the tumor by increasing the tumor/blood ratio of the polyethylene glycol containing compound.
摘要翻译：公开了一种新的抗聚乙二醇单克隆抗体及其制备方法。这样的抗体可以用于体外测定聚乙二醇浓度，或加速来自人体血液循环的含聚乙二醇的化合物的清除，从而降低与含聚乙二醇的缀合物相关的毒性。抗体特别可用于癌症治疗，其中治疗剂通过增加含聚乙二醇的化合物的肿瘤/血液比选择性递送至肿瘤。

5. 发明申请

US20130217133A1 AUTO-DEVELOPING AND REGULARLY-WEIGHTED PROTEIN MOLECULAR WEIGHT MARKER KIT AND METHOD FOR PREPARING THE SAME 有权
标题翻译：自动发展和定量加权蛋白分子量标记试剂盒及其制备方法
公开(公告)号：US20130217133A1
公开(公告)日：2013-08-22
申请号：US13402086
申请日：2012-02-22
申请人： Tian-Lu Cheng , Chiu-Min Cheng
发明人： Tian-Lu Cheng , Chiu-Min Cheng
IPC分类号： G01N27/26 , C12P21/02
CPC分类号： G01N33/68
摘要： The present invention provides an auto-developing and regularly-weighted protein molecular weight marker kit, comprising: (a) a plurality of recombinant proteins having formula (I), (B)m-A-(C)n (I), wherein A is a polypeptide of SEQ ID NO: 1, B and C are independently any mutually identical or different polypeptides with the value of molecular weight being a multiple of 5, and m and n is independently 0 or any integer larger than 0; and (b) one or more solvents for stabilizing the recombinant proteins. The present invention also provides a method for preparing the auto-developing and regularly-weighted protein molecular weight marker kit.
摘要翻译：本发明提供了一种自动发展和规则加权的蛋白质分子量标记试剂盒，其包括：（a）多种具有式（I），（B）mA-（C）n（I）的重组蛋白，其中A为 SEQ ID NO：1，B和C的多肽独立地是任何相互相同或不同的多肽，分子量的值是5的倍数，m和n独立地为0或大于0的整数; 和（b）用于稳定重组蛋白质的一种或多种溶剂。本发明还提供了一种制备自动显影和定量加权的蛋白质分子量标记试剂盒的方法。

6. 发明授权

US06617118B2 Monoclonal antibody for analysis and clearance of polyethylene glycol and polyethylene glycol-modified molecules 有权
标题翻译：用于分析和清除聚乙二醇和聚乙二醇改性分子的单克隆抗体
公开(公告)号：US06617118B2
公开(公告)日：2003-09-09
申请号：US09810379
申请日：2001-03-16
申请人： Steve Roffler , Tian-Lu Cheng , Pin-Yi Wu
发明人： Steve Roffler , Tian-Lu Cheng , Pin-Yi Wu
IPC分类号： G01N3353
CPC分类号： G01N33/5308 , A61K2039/505 , C07K16/44 , G01N2430/60
摘要： A novel anti-polyethylene glycol monoclonal antibody and its preparation are disclosed. Such an antibody can be used for determining polyethylene glycol concentration in vitro or accelerating the clearance of a polyethylene glycol containing compound from the blood circulation in the human body thereby reducing the toxicity associated with the polyethylene glycol containing conjugate. The antibody is particularly useful in cancer therapy where the therapeutic agent is selectively delivered to the tumor by increasing the tumor/blood ratio of the polyethylene glycol containing compound.
摘要翻译：公开了一种新的抗聚乙二醇单克隆抗体及其制备方法。这样的抗体可以用于体外测定聚乙二醇浓度，或加速来自人体血液循环的含聚乙二醇的化合物的清除，从而降低与含聚乙二醇的缀合物相关的毒性。抗体特别可用于癌症治疗，其中治疗剂通过增加含聚乙二醇的化合物的肿瘤/血液比选择性递送至肿瘤。

7. 发明申请

US20060068396A1 Method for biochip detecting limited cells 审中-公开
标题翻译：生物芯片检测有限细胞的方法
公开(公告)号：US20060068396A1
公开(公告)日：2006-03-30
申请号：US10948224
申请日：2004-09-24
申请人： Shiu-Ru Lin , Tian-Lu Cheng , Yi-Fang Chen , Chan-Han Wu
发明人： Shiu-Ru Lin , Tian-Lu Cheng , Yi-Fang Chen , Chan-Han Wu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q2565/507
摘要： The present invention is a method for a biochip detecting limited cells. The present invention comprises steps of: obtaining a nylon membrane chip having required nucleotide fragments arranged in a dot matrix way by using a manual spotter; naturally drying by heat and fixing the nucleotide fragments on the nylon membrane chip by a rapid nucleic acid cross-linker when preparing a chip; collecting some normal whole blood to be linearly amplified; synthesizing required amount of cDNA by a reverse transcription to obtain a marker as a probe; processing labeling, hybridization and post-hybridization to the chip and the marker; processing chemical color reaction; and automatically analyzing the result image after the chemical color reaction. Accordingly, a gene biochip operation technology platform with low cost, easy operation and high efficiency is obtained. And so the functions and the applications of the gene biochip can be effectively worked out and the practical applications of the gene biochip on related fields can be conclusively popularized.
摘要翻译：本发明是一种检测有限细胞的生物芯片的方法。本发明包括以下步骤：通过使用手动打斑机获得具有以点阵方式布置的所需核苷酸片段的尼龙膜芯片; 通过加热自然干燥，并在制备芯片时通过快速核酸交联剂固定尼龙膜芯片上的核苷酸片段; 收集一些正常全血要线性扩增; 通过逆转录合成所需量的cDNA以获得作为探针的标记; 加工标记，杂交和后期杂交到芯片和标记物; 加工化学色素反应; 并在化学反应后自动分析结果图像。因此，获得了低成本，易于操作和高效率的基因生物芯片操作技术平台。因此可以有效地制定基因生物芯片的功能和应用，基因生物芯片在相关领域的实际应用可以得到最终推广。

8. 发明授权

US08791240B2 Auto-developing and regularly-weighted protein molecular weight marker kit and method for preparing the same 有权
标题翻译：自动开发和定时加权的蛋白质分子量标记试剂盒及其制备方法
公开(公告)号：US08791240B2
公开(公告)日：2014-07-29
申请号：US13402086
申请日：2012-02-22
申请人： Tian-Lu Cheng , Chiu-Min Cheng
发明人： Tian-Lu Cheng , Chiu-Min Cheng
IPC分类号： C07K1/00 , C12P21/04
CPC分类号： G01N33/68
摘要： The present invention provides an auto-developing and regularly-weighted protein molecular weight marker kit, comprising: (a) a plurality of recombinant proteins having formula (I), (B)m-A-(C)n (I), wherein A is a polypeptide of SEQ ID NO: 1, B and C are independently any mutually identical or different polypeptides with the value of molecular weight being a multiple of 5, and m and n is independently 0 or any integer larger than 0; and (b) one or more solvents for stabilizing the recombinant proteins. The present invention also provides a method for preparing the auto-developing and regularly-weighted protein molecular weight marker kit.
摘要翻译：本发明提供了一种自动发展和规则加权的蛋白质分子量标记试剂盒，其包括：（a）多种具有式（I），（B）mA-（C）n（I）的重组蛋白，其中A为 SEQ ID NO：1，B和C的多肽独立地是任何相互相同或不同的多肽，分子量的值是5的倍数，m和n独立地为0或大于0的整数; 和（b）用于稳定重组蛋白质的一种或多种溶剂。本发明还提供了一种制备自动显影和定量加权的蛋白质分子量标记试剂盒的方法。

9. 发明申请

US20080176225A1 MEMBRANE BOUND REPORTER GENE SYSTEM 审中-公开
标题翻译：膜结合报告基因系统
公开(公告)号：US20080176225A1
公开(公告)日：2008-07-24
申请号：US11624625
申请日：2007-01-18
申请人： Steve ROFFLER , Tian-Lu Cheng , Yu-Cheng Su
发明人： Steve ROFFLER , Tian-Lu Cheng , Yu-Cheng Su
IPC分类号： C12Q1/68 , C07H21/04 , C12N15/55 , C12N15/85
CPC分类号： C12Y302/01031 , A01K2227/105 , A01K2267/0393 , C12N9/2434 , C12N15/65 , C12N15/86 , C12N2740/13043 , C12Q1/6897
摘要： A recombinant DNA construct is provided and includes a first DNA fragment encoding a β-glucuronidase and a second DNA fragment encoding a membrane anchoring domain. The β-glucuronidase may be a human β-glucuronidase or a mouse β-glucuronidase. In one embodiment, an expression vector for delivering a gene of interest or portion thereof into a host cell includes a DNA sequence for the gene of interest, a first DNA fragment encoding a β-glucuronidase, and a second DNA fragment encoding a membrane anchoring domain. In another embodiment, a method of introducing a gene of interest or portion thereof into a host cell is provided, including introducing into the host cell a recombinant DNA construct.
摘要翻译：提供重组DNA构建体并且包括编码β-葡糖醛酸糖苷酶的第一DNA片段和编码膜锚定结构域的第二DNA片段。 β-葡糖苷酸酶可以是人β-葡糖醛酸糖苷酶或小鼠β-葡糖醛酸糖苷酶。在一个实施方案中，用于将感兴趣的基因或其部分递送到宿主细胞中的表达载体包括目的基因的DNA序列，编码β-葡糖醛酸糖苷酶的第一DNA片段和编码膜锚定结构域的第二DNA片段。在另一个实施方案中，提供将感兴趣的基因或其部分引入宿主细胞的方法，包括向宿主细胞中引入重组DNA构建体。

10. 发明申请

US20080050729A1 Complex cancer cells luminescence detection method 审中-公开
标题翻译：复合癌细胞发光检测方法
公开(公告)号：US20080050729A1
公开(公告)日：2008-02-28
申请号：US11585974
申请日：2006-10-25
申请人： Shiu-Ru Lin , Tian-Lu Cheng
发明人： Shiu-Ru Lin , Tian-Lu Cheng
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837
摘要： A method for detecting cancer cells. A chemical luminescence reaction is used to obtain a result image. The result image is analyzed through a weighting process to a value of each gene. By doing do, a cancer can be precisely diagnosed.
摘要翻译：一种检测癌细胞的方法。使用化学发光反应来获得结果图像。通过加权过程对每个基因的值分析结果图像。通过这样做，可以精确诊断癌症。

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