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1. 发明授权

US09447150B2 Peptide domains that bind small molecules of industrial significance 有权
标题翻译：结合小分子工业意义的肽结构域
公开(公告)号：US09447150B2
公开(公告)日：2016-09-20
申请号：US13575834
申请日：2011-01-28
申请人： Thomas P. Binder , Aragula Gururaj Rao , Yasufumi Yamamoto , Paul D. Hanke
发明人： Thomas P. Binder , Aragula Gururaj Rao , Yasufumi Yamamoto , Paul D. Hanke
IPC分类号： C07K7/06 , C40B30/06 , C07K14/00 , C07K7/08
CPC分类号： C07K7/06 , C07K5/08 , C07K7/08 , C07K14/00
摘要： Described herein are small peptide domains and consensus sequences that bind small target molecules of industrial importance, e.g., metals such as nickel, β carotene, and isoflavones such as genistein. Also described are fusion proteins containing such binding domains fused to proteins or to peptide domains like GST or CBD that bind other ligands and can be used to immobilize the target binding domain on a support. One class of fusion proteins that is useful in industrial settings are fusions that contain concatemers of target binding domains, which increases the binding equivalents per molecule.
摘要翻译：本文描述的是结合工业重要的小靶分子的小肽结构域和共有序列，例如金属如镍，β-胡萝卜素和异黄酮如染料木素。还描述了融合蛋白，其含有融合蛋白质或结合其它配体的肽结构域（如GST或CBD）的融合蛋白，并可用于将靶结合结构域固定在载体上。在工业环境中有用的一类融合蛋白是融合靶结合域的融合物，这增加了每个分子的结合当量。

2. 发明授权

US07094582B2 Cell-free production of glucosamine 失效
标题翻译：无细胞生产葡糖胺
公开(公告)号：US07094582B2
公开(公告)日：2006-08-22
申请号：US10671886
申请日：2003-09-29
申请人： Wuli Bao , Thomas P. Binder , Paul D. Hanke , Leif Solheim
发明人： Wuli Bao , Thomas P. Binder , Paul D. Hanke , Leif Solheim
IPC分类号： C12P19/28 , C07H5/06
CPC分类号： C12P19/26
摘要： Methods for cell-free production of glucosamine from starch, maltodextrin or glycogen or from fructose and a source of amino groups are disclosed. Also disclosed are cellular extracts comprising glucosamine-6-phosphate synthase activity, as well as a cellular extract comprising glucosamine-6-phosphate deaminase.
摘要翻译：公开了从淀粉，麦芽糖糊精或糖原或果糖和氨基来源生产葡糖胺的无细胞方法。还公开了包含葡糖胺-6-磷酸合酶活性的细胞提取物，以及包含葡糖胺-6-磷酸脱氨酶的细胞提取物。

3. 发明授权

US5939307A Strains of Escherichia coli, methods of preparing the same and use thereof in fermentation processes for l-threonine production 失效
标题翻译：大肠杆菌菌株，其制备方法及其在L-苏氨酸生产的发酵过程中的应用
公开(公告)号：US5939307A
公开(公告)日：1999-08-17
申请号：US902336
申请日：1997-07-29
申请人： Ming-Der Wang , Jill S. Bradshaw , Stacia L. Swisher , Hungming James Liaw , Paul D. Hanke , Thomas P. Binder
发明人： Ming-Der Wang , Jill S. Bradshaw , Stacia L. Swisher , Hungming James Liaw , Paul D. Hanke , Thomas P. Binder
IPC分类号： C12N15/09 , C12N1/21 , C12N15/52 , C12P13/08 , C12R1/19 , C12N1/20
CPC分类号： C12Y101/01003 , C12N15/52 , C12P13/08 , C12Y207/01039 , C12Y207/02004 , C12Y402/03001
摘要： The present invention relates to novel strains of Escherichia coli and fermentation processes involving these microorganisms. More specifically, the present invention relates to genetically-modified Escherichia coli strains and the use thereof for the production of the amino acids, particularly members of the aspartate family of amino acids such as threonine. The present invention also relates to methods of preparing E. coli strains for use in the fermentative production of amino acids.
摘要翻译：本发明涉及大肠杆菌的新菌株和涉及这些微生物的发酵方法。更具体地，本发明涉及遗传修饰的大肠杆菌菌株及其用于生产氨基酸，特别是天冬氨酸家族氨基酸家族如苏氨酸的成员的用途。本发明还涉及制备用于发酵生产氨基酸的大肠杆菌菌株的方法。

4. 发明申请

US20090081740A1 PRODUCTION OF AMINO ACIDS FROM SUCROSE IN CORYNEBACTERIUM GLUTAMICUM 有权
标题翻译：在玉米淀粉中生产氨基酸
公开(公告)号：US20090081740A1
公开(公告)日：2009-03-26
申请号：US12239242
申请日：2008-09-26
申请人： Thomas P. Binder , Paul D. Hanke
发明人： Thomas P. Binder , Paul D. Hanke
IPC分类号： C12P13/08 , C12P13/04 , C12N1/21
CPC分类号： C12P13/04 , C12P13/08 , C12R1/15
摘要： Methods and compositions for increased production of amino acids from C. glutamicum using sucrose as a carbon source are described. In one aspect, increased production of L-lysine from C. glutamicum is accomplished by using a strain having a mutation in the ptsF gene encoding fructose-PTS enzyme that attenuates or blocks fructose import into the cell when such strain is grown on media containing sucrose as a carbon source and production is increased by providing glucose isomerase in the fermentation media. The glucose isomerase may be exogenously added or expressed in the strain and exported into the media. In certain embodiments the media also contain an invertase. In another aspect increased production of L-lysine is accomplished by making a C. glutamicum strain having the ptsF mutation and a second mutation in a fructose exporter function. The dual mutation retains imported fructose in the cell. In certain embodiments, the strain also overexpresses at least one of a glucose isomerase and glucokinase activity in the cell to drive imported fructose toward the pentose phosphate pathway to increase L-lysine production.
摘要翻译：描述了使用蔗糖作为碳源来增加谷氨酸棒球菌产生氨基酸的方法和组合物。一方面，通过使用编码果糖-PTS酶的ptsF基因中的突变的菌株，当将这种菌株在含有蔗糖的培养基上生长时，使果糖进入细胞，从而导致来自谷氨酸棒球菌的L-赖氨酸的生产增加作为碳源，通过在发酵培养基中提供葡萄糖异构酶来增加产量。葡萄糖异构酶可以在菌株中外源加入或表达并输出到培养基中。在某些实施方案中，培养基还含有转化酶。另一方面，通过制备具有ptsF突变和果糖出口功能中的第二突变的谷氨酸棒杆菌菌株来实现L-赖氨酸的生产增加。双重突变在细胞中保留了进口的果糖。在某些实施方案中，菌株也在细胞中过表达葡萄糖异构酶和葡萄糖激酶活性中的至少一种，以驱动进口的果糖向戊糖磷酸途径增加L-赖氨酸生产。

5. 发明授权

US08048649B2 Production of amino acids from sucrose in Corynebacterium glutamicum 有权
标题翻译：从谷氨酸棒杆菌中的蔗糖生产氨基酸
公开(公告)号：US08048649B2
公开(公告)日：2011-11-01
申请号：US12239242
申请日：2008-09-26
申请人： Thomas P. Binder , Paul D. Hanke
发明人： Thomas P. Binder , Paul D. Hanke
IPC分类号： C12P13/08 , C12N9/12 , C12N9/22 , C12N1/21
CPC分类号： C12P13/04 , C12P13/08 , C12R1/15
摘要： Methods and compositions for increased production of amino acids from C. glutamicum using sucrose as a carbon source are described. In one aspect, increased production of L-lysine from C. glutamicum is accomplished by using a strain having a mutation in the ptsF gene encoding fructose-PTS enzyme that attenuates or blocks fructose import into the cell when such strain is grown on media containing sucrose as a carbon source and production is increased by providing glucose isomerase in the fermentation media. The glucose isomerase may be exogenously added or expressed in the strain and exported into the media. In certain embodiments the media also contain an invertase. In another aspect increased production of L-lysine is accomplished by making a C. glutamicum strain having the ptsF mutation and a second mutation in a fructose exporter function. The dual mutation retains imported fructose in the cell. In certain embodiments, the strain also overexpresses at least one of a glucose isomerase and glucokinase activity in the cell to drive imported fructose toward the pentose phosphate pathway to increase L-lysine production.
摘要翻译：描述了使用蔗糖作为碳源来增加谷氨酸棒球菌产生氨基酸的方法和组合物。一方面，通过使用编码果糖-PTS酶的ptsF基因中的突变的菌株，当将这种菌株在含有蔗糖的培养基上生长时，使果糖进入细胞，从而导致来自谷氨酸棒球菌的L-赖氨酸的生产增加作为碳源，通过在发酵培养基中提供葡萄糖异构酶来增加产量。葡萄糖异构酶可以在菌株中外源加入或表达并输出到培养基中。在某些实施方案中，培养基还含有转化酶。另一方面，通过制备具有ptsF突变和果糖出口功能中的第二突变的谷氨酸棒杆菌菌株来实现L-赖氨酸的生产增加。双重突变在细胞中保留了进口的果糖。在某些实施方案中，菌株也在细胞中过表达葡萄糖异构酶和葡萄糖激酶活性中的至少一种，以驱动进口的果糖向戊糖磷酸途径增加L-赖氨酸生产。

6. 发明授权

US06680190B2 Gene encoding phosphoglucoisomerase 有权
标题翻译：基因编码磷酸葡萄糖异构酶
公开(公告)号：US06680190B2
公开(公告)日：2004-01-20
申请号：US10223355
申请日：2002-08-20
申请人： Paul D. Hanke
发明人： Paul D. Hanke
IPC分类号： C12N992
CPC分类号： C12N9/0006 , C12N9/18 , C12N9/92 , C12P13/08
摘要： The present invention relates, in general, to a method of producing L-amino acids comprising culturing altered bacterial cells having increased amounts of NADPH as compared to unaltered bacterial cells whereby L-amino acids yields from said altered bacterial cells are greater than yields from unaltered bacterial cells. The invention also relates to a gene encoding phosphoglucoisomerase.
摘要翻译：本发明一般涉及生产L-氨基酸的方法，其包括与未改变的细菌细胞相比培养具有增加的NADPH量的改变的细菌细胞，由此来自所述改变的细菌细胞的L-氨基酸产量大于未改变的产量细菌细胞。本发明还涉及编码磷酸葡糖聚糖异构酶的基因。

7. 发明授权

US06927046B1 Increased lysine production by gene amplification using coryneform bacteria 有权
标题翻译：通过使用棒状细菌的基因扩增增加赖氨酸生产
公开(公告)号：US06927046B1
公开(公告)日：2005-08-09
申请号：US09722441
申请日：2000-11-28
申请人： Paul D. Hanke , Lhing-Yew Li-D'Elia , Holly J. Walsh , Corey M. Crafton , P. John Rayapati
发明人： Paul D. Hanke , Lhing-Yew Li-D'Elia , Holly J. Walsh , Corey M. Crafton , P. John Rayapati
IPC分类号： C12N15/09 , C07K14/34 , C07K14/345 , C12N1/21 , C12N9/12 , C12N9/88 , C12N15/52 , C12N15/54 , C12P13/04 , C12P13/08 , C12R1/13
CPC分类号： C12N9/1217 , C07K14/34 , C07K14/345 , C12N9/88 , C12N15/52 , C12P13/04 , C12P13/08
摘要： The invention provides methods to increase the production of an amino acid from Corynebacterium species by way of the amplification of amino acid biosynthetic pathway genes in a host cell chromosome. In a preferred embodiment, the invention provides methods to increase the production of L-lysine in Corynebacterium glutamicum by way of the amplification of L-lysine biosynthetic pathway genes in a host cell chromosome. The invention also provides novel processes for the production of an amino acid by way of the amplification of amino acid biosynthetic pathway genes in a host cell chromosome and/or by increasing promoter strength. In a preferred embodiment, the invention provides processes to increase the production of L-lysine in Corynebacterium glutamicum by way of the amplification of L-lysine biosynthetic pathway genes in a host cell chromosome. The invention also provides novel isolated nucleic acid molecules for L-lysine biosynthetic pathway genes of Corynebacterium glutamicum such as a naturally occurring, feedback-sensitive form of aspartokinase (ask) resulting from a threonine to isoleucine mutation at amino acid residue 380 in the ask gene of ATCC 21529, aspartate-semialdehyde dehydrogenase (asd), dihydrodipicolinate synthase (dapA), dihydrodipicolinate reductase (dapB), diaminopimelate dehydrogenase (ddh), and diaminopimelate decarboxylase (lysA).
摘要翻译：本发明提供了通过在宿主细胞染色体中扩增氨基酸生物合成途径基因来增加棒状杆菌属物种的氨基酸生产的方法。在优选的实施方案中，本发明提供了通过在宿主细胞染色体中扩增L-赖氨酸生物合成途径基因来增加谷氨酸棒杆菌中L-赖氨酸生产的方法。本发明还提供了通过在宿主细胞染色体中扩增氨基酸生物合成途径基因和/或通过增加启动子强度来生产氨基酸的新方法。在优选的实施方案中，本发明提供了通过在宿主细胞染色体中扩增L-赖氨酸生物合成途径基因来增加谷氨酸棒杆菌中L-赖氨酸生产的方法。本发明还提供了用于谷氨酸棒杆菌的L-赖氨酸生物合成途径基因的新型分离的核酸分子，例如在询问基因中的氨基酸残基380处的苏氨酸至异亮氨酸突变引起的天然存在的反馈敏感形式的天冬氨酸激酶（ask）（dapA），二氢吡啶二羧酸还原酶（dapB），二氨基庚二酸脱氢酶（ddh）和二氨基庚二酸脱羧酶（lysA）的天冬氨酸 - 半醛脱氢酶（asd），二氢吡啶二羧酸合酶（dapA）。

8. 发明授权

US06465238B1 Gene encoding phosphoglucoisomerase 有权
标题翻译：基因编码磷酸葡萄糖异构酶
公开(公告)号：US06465238B1
公开(公告)日：2002-10-15
申请号：US09621451
申请日：2000-07-21
申请人： Paul D. Hanke
发明人： Paul D. Hanke
IPC分类号： C12N992
CPC分类号： C12N9/0006 , C12N9/18 , C12N9/92 , C12P13/08
摘要： The present invention relates, in general, to a method of producing L-amino acids comprising culturing altered bacterial cells having increased amounts of NADPH as compared to unaltered bacterial cells whereby L-amino acids yields from said altered bacterial cells are greater than yields from unaltered bacterial cells. The invention also relates to a gene encoding phosphoglucoisomerase.
摘要翻译：本发明一般涉及生产L-氨基酸的方法，其包括与未改变的细菌细胞相比培养具有增加的NADPH量的改变的细菌细胞，由此来自所述改变的细菌细胞的L-氨基酸产量大于未改变的产量细菌细胞。本发明还涉及编码磷酸葡糖聚糖异构酶的基因。

9. 发明授权

US08067210B2 Method of producing lysine by culturing a host cell expressing a polynucleotide encoding a feedback resistant aspartokinase from corynebacterium 有权
标题翻译：通过培养表达编码来自棒状杆菌的反馈抗性天冬氨酸激酶的多核苷酸的宿主细胞产生赖氨酸的方法
公开(公告)号：US08067210B2
公开(公告)日：2011-11-29
申请号：US12772538
申请日：2010-05-03
申请人： Paul D. Hanke , Lhing-Yew Li-D'Elia , Holly J. Walsh
发明人： Paul D. Hanke , Lhing-Yew Li-D'Elia , Holly J. Walsh
IPC分类号： C12P13/04 , C12P1/00 , C12N1/20 , C07H21/02
CPC分类号： C12N9/1217 , C07K14/34 , C07K14/345 , C12N9/88 , C12N15/52 , C12P13/04 , C12P13/08
摘要： The invention provides methods to increase the production of an amino acid from Corynebacterium species by way of the amplification of amino acid biosynthetic pathway genes in a host cell chromosome. The invention also provides novel processes for the production of an amino acid by way of the amplification of amino acid biosynthetic pathway genes in a host cell chromosome and/or by increasing promoter strength. In a preferred embodiment, the invention provides processes to increase the production of L-lysine in Corynebacterium glutamicum by way of the amplification of L-lysine biosynthetic pathway genes in a host cell chromosome. The invention also provides novel isolated nucleic acid molecules for L-lysine biosynthetic pathway genes of Corynebacterium glutamicum.
摘要翻译：本发明提供了通过在宿主细胞染色体中扩增氨基酸生物合成途径基因来增加棒状杆菌属物种的氨基酸生产的方法。本发明还提供了通过在宿主细胞染色体中扩增氨基酸生物合成途径基因和/或通过增加启动子强度来生产氨基酸的新方法。在优选的实施方案中，本发明提供了通过在宿主细胞染色体中扩增L-赖氨酸生物合成途径基因来增加谷氨酸棒杆菌中L-赖氨酸生产的方法。本发明还提供了用于谷氨酸棒杆菌的L-赖氨酸生物合成途径基因的新型分离的核酸分子。

10. 发明授权

US07300777B2 Feedback-resistant pyruvate carboxylase gene from corynebacterium 有权
标题翻译：来自棒状杆菌的反馈抗性丙酮酸羧化酶基因
公开(公告)号：US07300777B2
公开(公告)日：2007-11-27
申请号：US11136887
申请日：2005-05-25
申请人： Paul D. Hanke , Anthony J. Sinskey , Laura B. Willis , Stephane Guillouet
发明人： Paul D. Hanke , Anthony J. Sinskey , Laura B. Willis , Stephane Guillouet
IPC分类号： C12P13/08 , C12P13/24 , C12P13/14 , C12P13/12 , C12P13/10 , C12P13/06 , C07H21/04 , C12N9/00
CPC分类号： C12N9/93 , C12P13/08 , C12Y604/01001
摘要： The present invention relates to a mutated pyruvate carboxylase gene from Corynebacterium. The mutant pyruvate carboxylase gene encodes a pyruvate carboxylase enzyme which is resistant to feedback inhibition from aspartic acid. The present invention also relates to a method of replacing the wild-type pyruvate carboxylase gene in Corynebacterium with this feedback-resistant pyruvate carboxylase gene. The present invention further relates to methods of the production of amino acids, preferably lysine, comprising the use of this mutant pyruvate carboxylase enzyme in microorganisms.
摘要翻译：本发明涉及来自棒状杆菌的突变型丙酮酸羧化酶基因。突变体丙酮酸羧化酶基因编码丙酮酸羧化酶，其对天冬氨酸的反馈抑制具有抗性。本发明还涉及用该反馈抗性丙酮酸羧化酶基因替代棒状杆菌中的野生型丙酮酸羧化酶基因的方法。本发明还涉及生产氨基酸，优选赖氨酸的方法，其包括在微生物中使用该突变型丙酮酸羧化酶。

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