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1. 发明申请

US20130211136A1 COMPOSITIONS COMPRISING SHIKIMIC ACID OBTAINED FROM OIL PALM BASED MATERIALS AND METHOD OF PRODUCING THEREOF 审中-公开
标题翻译：包含从基于棕榈的材料获得的活性酸的组合物及其生产方法
公开(公告)号：US20130211136A1
公开(公告)日：2013-08-15
申请号：US13704616
申请日：2011-06-16
申请人： Sambandan Tg , ChoKyun Rha , Ravigadevi Sambanthamurthi , Anthony J. Sinskey , Yew Ai Tan , Kalyana Sundram P. Manickam , Mohd Basri Wahid
发明人： Sambandan Tg , ChoKyun Rha , Ravigadevi Sambanthamurthi , Anthony J. Sinskey , Yew Ai Tan , Kalyana Sundram P. Manickam , Mohd Basri Wahid
IPC分类号： C07C51/42
CPC分类号： C07C51/50 , C07C51/42 , C07C51/48 , C07C2601/16 , C12N9/1092 , C12Y205/01019 , C07C62/32
摘要： The present invention provides compositions and method for production of shikimic acid based on extracts obtained from oil palm-based materials, and more particularly oil palm based waste materials and by-products. The method includes purifying shikimic acid from extracts comprising oil palm phenolics (OPP).
摘要翻译：本发明提供了基于由油棕基材料提取的提取物，特别是油棕基废料和副产物的莽草酸生产组合物和方法。该方法包括从包含油棕榈酚（OPP）的提取物中纯化莽草酸。

2. 发明授权

US06528706B1 Polyhydroxybutyrate polymerase 失效
标题翻译：聚羟基丁酸聚合酶
公开(公告)号：US06528706B1
公开(公告)日：2003-03-04
申请号：US09935244
申请日：2001-08-21
申请人： Oliver P. Peoples , Anthony J. Sinskey
发明人： Oliver P. Peoples , Anthony J. Sinskey
IPC分类号： A01H500
CPC分类号： C12N9/0006 , C12N9/00 , C12N9/1029 , C12N15/52 , C12P7/625
摘要： A method for controlling and modifying biopolymer synthesis by manipulation of the genetics and enzymology of synthesis of polyhydroxybutyrate (PHB) and polyhydroxyalkanoate (PHA) polyesters at the molecular level in procaryotic and eukaryotic cells, especially plants. Examples demonstrate the isolation, characterization, and expression of the genes involved in the production of PHB and PHA polymers. Genes encoding the enzymes in the PHB and PHA synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB polymerase or PHA polymerase) from Zoogloea ramigera strain I-16-M, Alcaligenes eutrophus, Nocardia salmonicolur, and Psuedomonas olevarans were identified or isolated and expressed in a non-PHB producing organism, E. coli. Specific modifications to the polymers include variation in the chain length of the polymers and incorporation of different monomers into the polymers to produce co-polymers with different physical properties.
摘要翻译：通过在原核和真核细胞，特别是植物中的分子水平处理聚羟基丁酸酯（PHB）和聚羟基链烷酸酯（PHA）聚酯的遗传学和酶学方法来控制和改变生物聚合物合成的方法。实施例证明参与PHB和PHA聚合物生产的基因的分离，表征和表达。鉴定或分离来自Zoogloea苎麻菌株I-16-M，产碱假单胞菌，痢疾杆菌（Nocardia salmonicolur）和橄榄油（Psuedomonas olevarans）的PHB和PHA合成途径（β-酮硫解酶，乙酰乙酰辅酶A还原酶和PHB聚合酶或PHA聚合酶）中的酶编码基因并在非PHB生物体大肠杆菌中表达。对聚合物的具体修饰包括聚合物链长度的变化以及将不同单体引入到聚合物中以产生具有不同物理性质的共聚物。

3. 发明授权

US5661026A Gene encoding bacterial beta-ketothiolase 失效
标题翻译：基因编码细菌β-酮硫解酶
公开(公告)号：US5661026A
公开(公告)日：1997-08-26
申请号：US435083
申请日：1995-05-04
申请人： Oliver P. Peoples , Anthony J. Sinskey
发明人： Oliver P. Peoples , Anthony J. Sinskey
IPC分类号： C12N9/00 , C12N9/04 , C12N9/10 , C12N15/52 , C12P7/62 , C12N15/54 , C12N15/74 , C12N15/75
CPC分类号： C12N9/0006 , C12N15/52 , C12N9/00 , C12N9/1029 , C12P7/625
摘要： The present invention is a method for controlling biopolymer synthesis by determining the genetics and enzymology of polyhydroxybutyrate (PHB) biosynthesis at the molecular level. The purified enzymes and genes provide the means for developing new PHB-like biopolymers having polyester backbones. Specific aims are to 1) control the chain length of the polymers produced in fermentation processes through genetic manipulation, 2) incorporate different monomers into the polymers to produce co-polymers with different physical properties, and 3) examine the physical/rheological properties of these new biopolymers in order to develop further design criteria at the molecular level.The method for engineering biopolymer synthesis includes: isolation and characterization of the genes for the enzymes in the synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB synthetase); cloning of the genes in a vector(s); placement of the vector(s) under the control of regulated promoters; expression of the genes; determination of the function and use of other factors such as substrate specificity in polymer production and composition; and isolation and physical and chemical analysis of the resulting polymers.
摘要翻译：本发明是通过在分子水平上测定聚羟基丁酸（PHB）生物合成的遗传学和酶学方法来控制生物聚合物合成的方法。纯化的酶和基因为开发具有聚酯主链的新型PHB样生物聚合物提供了手段。具体目的是1）通过遗传操作来控制在发酵过程中产生的聚合物的链长; 2）将不同的单体引入到聚合物中以产生具有不同物理性质的共聚物，以及3）检查这些物质/流变性质新的生物聚合物，以便在分子水平上制定进一步的设计标准。工程生物聚合物合成的方法包括：合成途径中酶的分离和表征（β-酮硫解酶，乙酰乙酰辅酶A还原酶和PHB合成酶）; 在载体中克隆基因; 将载体置于受调节启动子的控制下; 基因表达; 确定聚合物生产和组成中其他因素如底物特异性的功能和用途; 以及所得聚合物的分离和物理和化学分析。

4. 发明授权

US5512669A Gene encoding bacterial acetoacetyl-COA reductase 失效
标题翻译：基因编码细菌乙酰乙酰辅酶A还原酶
公开(公告)号：US5512669A
公开(公告)日：1996-04-30
申请号：US297667
申请日：1994-08-29
申请人： Oliver P. Peoples , Anthony J. Sinskey
发明人： Oliver P. Peoples , Anthony J. Sinskey
IPC分类号： C12N9/00 , C12N9/04 , C12N9/10 , C12N15/52 , C12P7/62 , C12N15/53
CPC分类号： C12N9/0006 , C12N15/52 , C12N9/00 , C12N9/1029 , C12P7/625
摘要： The present invention is a method for controlling biopolymer synthesis by determining the genetics and enzymology of polyhydroxybutyrate (PHB) biosynthesis at the molecular level. The purified enzymes and genes provide the means for developing new PHB-like bicpolymers having polyester backbones. Specific aims are to 1) control the chain length of the polymers produced in fermentation processes through genetic manipulation, 2) incorporate different monomers into the polymers to produce co-polymers with different physical properties, and 3) examine the physical/rheological properties of these new biopolymers in order to develop further design criteria at the molecular level.The method for engineering biopolymer synthesis includes: isolation and characterization of the genes for the enzymes in the synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB synthetase); cloning of the genes in a vector(s); placement of the vector(s) under the control of regulated promoters; expression of the genes; determination of the function and use of other factors such as substrate specificity in polymer production and composition; and isolation and physical and chemical analysis of the resulting polymers.
摘要翻译：本发明是通过在分子水平上测定聚羟基丁酸（PHB）生物合成的遗传学和酶学方法来控制生物聚合物合成的方法。纯化的酶和基因提供了开发具有聚酯主链的新型PHB-类双聚物的手段。具体目的是1）通过遗传操作来控制在发酵过程中产生的聚合物的链长; 2）将不同的单体引入到聚合物中以产生具有不同物理性质的共聚物，以及3）检查这些物质/流变性质新的生物聚合物，以便在分子水平上制定进一步的设计标准。工程生物聚合物合成的方法包括：合成途径中酶的分离和表征（β-酮硫解酶，乙酰乙酰辅酶A还原酶和PHB合成酶）; 在载体中克隆基因; 将载体置于受调节启动子的控制下; 基因表达; 确定聚合物生产和组成中其他因素如底物特异性的功能和用途; 以及所得聚合物的分离和物理和化学分析。

5. 发明授权

US5506124A Process for preparing glucanase resistant yeast mutants 失效
标题翻译：制备葡聚糖酶抗性酵母突变体的方法
公开(公告)号：US5506124A
公开(公告)日：1996-04-09
申请号：US107497
申请日：1993-08-17
申请人： Spiros Jamas , ChoKyun Rha , Anthony J. Sinskey
发明人： Spiros Jamas , ChoKyun Rha , Anthony J. Sinskey
IPC分类号： C08B37/00 , C09K3/00 , C12P19/04 , C12N15/00 , C12N1/16 , C12N1/18 , C12Q1/34
CPC分类号： C12R1/865 , C08B37/0024 , C09K3/00 , C12P19/04 , Y10S435/942
摘要： Three dimensional glucan matrix compositions are prepared by separating growing yeast from its growth medium, subjecting the yeast with cell walls intact to an alkali material, thereby extracting whole glucan particles having an intact cell wall structure. The whole glucans can then, optionally, be treated with acetic acid to alter the .beta.(1-6) linkages, or with glucanase to alter the .beta.(1-3) linkages. The glucans have viscosity characteristics dependent upon the strain of yeast utilized and are useful as stabilizers or thickeners.
摘要翻译：通过从其生长培养基中分离生长的酵母，将酵母细胞壁完整地进行碱性物质制备三维葡聚糖基质组合物，从而提取具有完整细胞壁结构的全部葡聚糖颗粒。然后可以任选地用乙酸处理整个葡聚糖以改变β（1-6）键或用葡聚糖酶改变β（1-3）键。葡聚糖具有取决于所用酵母的菌株的粘度特性，并且可用作稳定剂或增稠剂。

6. 发明授权

US4992540A Glucan composition and process for preparation thereof 失效
公开(公告)号：US4992540A
公开(公告)日：1991-02-12
申请号：US297982
申请日：1989-01-17
申请人： Spiros Jamas , ChoKyun Rha , Anthony J. Sinskey
发明人： Spiros Jamas , ChoKyun Rha , Anthony J. Sinskey
IPC分类号： C08B37/00 , C09K3/00 , C12P19/04
CPC分类号： C12P19/04 , C08B37/0024 , C09K3/00
摘要： Three dimensional glucan matrix compositions are prepared by separating growing yeast from its growth medium, subjecting the yeast with cell walls intact to an alkali material, thereby extracting whole glucan particles having an intact cell wall structure. The whole glucans can then, optionally, be treated with acetic acid to alter the .beta.(1-6) linkages, or with glucanase to alter the .beta.(1-3) linkages. The glucans have viscosity characteristics dependent upon the strain of yeast utilized and are useful as stabilizers or thickeners.

7. 发明授权

US4948733A Zoogloea transformation using exopoly saccharide non-capsule producing strains 失效
标题翻译： Zoogloea转化使用外源糖非胶囊生产菌株
公开(公告)号：US4948733A
公开(公告)日：1990-08-14
申请号：US35604
申请日：1987-04-07
申请人： Donald D. Easson, Jr. , Oliver P. Peoples , Anthony J. Sinskey
发明人： Donald D. Easson, Jr. , Oliver P. Peoples , Anthony J. Sinskey
IPC分类号： C09K8/90 , C12N1/21 , C12N15/52 , C12P19/04
CPC分类号： C09K8/905 , C12N15/52 , C12P19/04
摘要： Two new bacterial strains designated Zoogloea ramigera 115SL and Zoogloea ramigera 115SLR, a rifampicin resistant derivative of 115SL, have been developed. These strains are derived from the wild type Zoogloea ramigera 115, ATCC 25935. The two new strains produce a novel exopolysaccharide (EPS) and have several desirable characteristics that are absent from the parent strain, including improved culture properties, since they do not produce an EPS capsule layer like that of the parent 115 strain. The 115SL EPS is instead excreted as a slime layer which is not confined to the immediate area surrounding the cells. Since cells are not trapped within a floc where they grow at a reduced rate or die because of nutrient starvation, the new strains have more consistent and reproducible growth cycles and increased growth rates. As a consequence, exopolysaccharide production is more consistent and titers are higher. The separation of the EPS from the cells is also much easier and more economical. The other very important characteristic of strains 115SL and 115SLR is that they are able to receive foreign DNA using conventional techniques due to the absence of the capsule layer. This facilitates the application of recombinant DNA technology to control and produce novel expolysaccharides.
摘要翻译：已经开发了两种新的细菌菌株，称为Zoogloea ramigera 115SL和Zoogloea ramigera 115SLR，115SL的利福平抗性衍生物。这些菌株衍生自野生型Zoogloea ramigera 115，ATCC 25935.两种新菌株产生新颖的外多糖（EPS），并具有几种所需的特征，其不存在于亲本菌株中，包括改良的培养性质，因为它们不产生 EPS胶囊层像母体115株。相反，115SL EPS排泄成为不限于细胞周围的紧邻区域的粘液层。由于细胞不被捕获在絮状物中，它们以降低的速率生长或由于营养物质饥饿而死亡，所以新菌株具有更一致和可重现的生长周期和增加的生长速率。结果，外多糖生产更一致，滴度更高。 EPS与细胞的分离也更容易和更经济。菌株115SL和115SLR的另一个非常重要的特征是，由于不存在胶囊层，它们能够使用常规技术接收外源DNA。这有利于重组DNA技术的应用来控制和生产新的多糖。

8. 发明授权

US08314071B2 Bioactive molecules from co-cultivation of microbes 有权
标题翻译：生物活性分子共培养微生物
公开(公告)号：US08314071B2
公开(公告)日：2012-11-20
申请号：US12599730
申请日：2008-12-22
申请人： Anthony J. Sinskey , Philip A. Lessard , Kazuhiro Kurosawa
发明人： Anthony J. Sinskey , Philip A. Lessard , Kazuhiro Kurosawa
IPC分类号： A61K31/70 , C07H17/02
CPC分类号： C07D498/10
摘要： Certain aspects of the invention relate to antibiotics, as well as pharmaceutically acceptable salts, pro-drugs and/or analogs thereof. Another aspect of the invention relates to methods of use of said antibiotics.
摘要翻译：本发明的某些方面涉及抗生素，以及其药学上可接受的盐，前药和/或其类似物。本发明的另一方面涉及使用所述抗生素的方法。

9. 发明申请

US20100249051A1 Bioactive Molecules from Co-Cultivation of Microbes 有权
标题翻译：微生物共培养的生物活性分子
公开(公告)号：US20100249051A1
公开(公告)日：2010-09-30
申请号：US12599730
申请日：2008-12-22
申请人： Anthony J. Sinskey , Philip A. Lessard , Kazuhiko Kurosawa
发明人： Anthony J. Sinskey , Philip A. Lessard , Kazuhiko Kurosawa
IPC分类号： A61K31/7036 , C07H15/238 , A61P31/04
CPC分类号： C07D498/10
摘要： Certain aspects of the invention relates to antibiotics, as well as pharmaceutically acceptable salts, pro-drugs and/or analogs thereof. Another aspect of the inventions relates to methods of use of said antibiotics.
摘要翻译：本发明的某些方面涉及抗生素，以及其药学上可接受的盐，前药和/或其类似物。本发明的另一方面涉及使用所述抗生素的方法。

10. 发明授权

US06884606B2 Pyruvate carboxylase from Corynebacterium glutamicum 有权
标题翻译：来自谷氨酸棒杆菌的丙酮酸羧化酶
公开(公告)号：US06884606B2
公开(公告)日：2005-04-26
申请号：US10045072
申请日：2002-01-15
申请人： Anthony J. Sinskey , Philip A. Lessard , Laura B. Willis
发明人： Anthony J. Sinskey , Philip A. Lessard , Laura B. Willis
IPC分类号： C12N1/21 , C12N9/00 , C12P13/08
CPC分类号： C12N9/93
摘要： The present invention concerns an anaplerotic enzyme from Corynebacterium glutamicum which replenishes oxaloacetate consumed during lysine and glutamic acid production in industrial fermentations. In particular, isolated nucleic acid molecules are provided encoding the pyruvate carboxylase protein. Pyruvate carboxylase polypeptides are also provided.
摘要翻译：本发明涉及谷氨酸棒状杆菌的一种厌食酶，其补充在工业发酵过程中在赖氨酸和谷氨酸生产期间消耗的草酰乙酸酯。特别地，提供编码丙酮酸羧化酶蛋白质的分离的核酸分子。还提供了丙酮酸羧化酶多肽。

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