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1. 发明授权

US08357490B2 Integrated instrument performing synthesis and amplification, and a system and method thereof 有权
标题翻译：进行合成和放大的集成仪器及其系统及方法
公开(公告)号：US08357490B2
公开(公告)日：2013-01-22
申请号：US12841708
申请日：2010-07-22
申请人： Thomas Froehlich , Martin Gutekunst , Dieter Heindl , Angelika Roesler , Rudolf Seibl
发明人： Thomas Froehlich , Martin Gutekunst , Dieter Heindl , Angelika Roesler , Rudolf Seibl
IPC分类号： C12Q1/68 , C12P19/34 , C40B60/02 , C40B60/04 , C40B60/08
CPC分类号： G01N35/028 , B01J19/0046 , B01J2219/00286 , B01J2219/00308 , B01J2219/00315 , B01J2219/00364 , B01J2219/00423 , B01J2219/00454 , B01J2219/00511 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00639 , B01J2219/00691 , B01J2219/00695 , B01J2219/00722 , B01L7/52 , C40B60/14 , G01N35/1002 , G01N2035/00326 , G01N2035/00356
摘要： An integrated instrument for oligonucleotide synthesis and PCR, and a system and method thereof are disclosed. The integrated instrument is basically composed of two independent modules. The first module is a unit for chemical de novo synthesis of oligonucleotides such as oligonucleotide primers and/or oligonucleotide hybridization probes. The second module is a unit for performing an analytical polymerase chain reaction amplification in real time, i.e. a qPCR. The two modules are operatively linked to each other in such a way that a user can load a nucleic sample to be analyzed into the integrated instrument and perform a PCR reaction by programming the instrument without a previous external synthesis of oligonucleotide amplification primers.
摘要翻译：公开了一种用于寡核苷酸合成和PCR的综合仪器及其系统及方法。综合仪器基本上由两个独立的模块组成。第一模块是寡核苷酸引物和/或寡核苷酸杂交探针等寡核苷酸的化学从头合成的单元。第二个模块是用于实时进行分析聚合酶链反应扩增的单元，即qPCR。两个模块可操作地彼此连接，使得用户可以将待分析的核酸样品加载到整合的仪器中，并且通过在没有先前的外部合成寡核苷酸扩增引物的情况下编程仪器进行PCR反应。

2. 发明申请

US20110177514A1 INTEGRATED INSTRUMENT PERFORMING SYNTHESIS AND AMPLIFICATION, AND A SYSTEM AND METHOD THEREOF 有权
标题翻译：一体化仪器进行合成和放大及其系统及方法
公开(公告)号：US20110177514A1
公开(公告)日：2011-07-21
申请号：US12841708
申请日：2010-07-22
申请人： Thomas Froehlich , Martin Gutekunst , Dieter Heindl , Angelika Roesler , Rudolf Seibl
发明人： Thomas Froehlich , Martin Gutekunst , Dieter Heindl , Angelika Roesler , Rudolf Seibl
IPC分类号： C12Q1/68 , C12M1/00 , C40B60/14 , C12M1/34
CPC分类号： G01N35/028 , B01J19/0046 , B01J2219/00286 , B01J2219/00308 , B01J2219/00315 , B01J2219/00364 , B01J2219/00423 , B01J2219/00454 , B01J2219/00511 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00639 , B01J2219/00691 , B01J2219/00695 , B01J2219/00722 , B01L7/52 , C40B60/14 , G01N35/1002 , G01N2035/00326 , G01N2035/00356
摘要： An integrated instrument for oligonucleotide synthesis and PCR, and a system and method thereof are disclosed. The integrated instrument is basically composed of two independent modules. The first module is a unit for chemical de novo synthesis of oligonucleotides such as oligonucleotide primers and/or oligonucleotide hybridization probes. The second module is a unit for performing an analytical polymerase chain reaction amplification in real time, i.e. a qPCR. The two modules are operatively linked to each other in such a way that a user can load a nucleic sample to be analyzed into the integrated instrument and perform a PCR reaction by programming the instrument without a previous external synthesis of oligonucleotide amplification primers.
摘要翻译：公开了一种用于寡核苷酸合成和PCR的综合仪器及其系统及方法。综合仪器基本上由两个独立的模块组成。第一模块是寡核苷酸引物和/或寡核苷酸杂交探针等寡核苷酸的化学从头合成的单元。第二个模块是用于实时进行分析聚合酶链反应扩增的单元，即qPCR。两个模块可操作地彼此连接，使得用户可以将待分析的核酸样品加载到整合的仪器中，并且通过在没有先前的外部合成寡核苷酸扩增引物的情况下编程仪器进行PCR反应。

3. 发明授权

US08822158B2 Miniaturized, high-throughput nucleic acid analysis 有权
标题翻译：小型化，高通量核酸分析
公开(公告)号：US08822158B2
公开(公告)日：2014-09-02
申请号：US13746812
申请日：2013-01-22
申请人： Thomas Froehlich , Dieter Heindl , Angelika Roesler , Tobias Heckel
发明人： Thomas Froehlich , Dieter Heindl , Angelika Roesler , Tobias Heckel
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6874 , C12Q1/6834 , C12Q1/6846 , C12Q2563/149 , C12Q2525/197 , C12Q2523/319 , C12Q2565/537 , C12Q2547/101 , C12Q2537/143
摘要： The present invention is directed to method for analyzing multiple nucleic acid molecules of interest comprising in the steps of (i) providing a plurality of beads, characterized in that each bead comprises at least two sequence specific amplification primers, further characterized in that at least one of the primers is bound to the bead via a cleavable linker, (ii) capturing the nucleic acid molecules of interest from a sample, (iii) clonally isolating the plurality of beads, (iv) cleaving the at least one primer, (v) clonally amplifying the nucleic acid thereby creating multiple amplification products, and (vi) analyzing the amplification products.
摘要翻译：本发明涉及用于分析感兴趣的多个核酸分子的方法，包括以下步骤：（i）提供多个珠粒，其特征在于每个珠粒包含至少两个序列特异性扩增引物，其特征还在于至少一个的引物通过可切割的接头与珠结合，（ii）从样品中捕获感兴趣的核酸分子，（iii）克隆分离多个珠，（iv）切割至少一个引物，（v）克隆扩增核酸从而产生多个扩增产物，和（vi）分析扩增产物。

4. 发明申请

US20130190191A1 MINIATURIZED, HIGH-THROUGHPUT NUCLEIC ACID ANALYSIS 有权
标题翻译：微型化，高通量核酸分析
公开(公告)号：US20130190191A1
公开(公告)日：2013-07-25
申请号：US13746812
申请日：2013-01-22
申请人： Thomas Froehlich , Dieter Heindl , Angelika Roesler
发明人： Thomas Froehlich , Dieter Heindl , Angelika Roesler
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6834 , C12Q1/6846 , C12Q2563/149 , C12Q2525/197 , C12Q2523/319 , C12Q2565/537 , C12Q2547/101 , C12Q2537/143
摘要： The present invention is directed to method for analyzing multiple nucleic acid molecules of interest comprising in the steps of (i) providing a plurality of beads, characterized in that each bead comprises at least two sequence specific amplification primers, further characterized in that at least one of the primers is bound to the bead via a cleavable linker, (ii) capturing the nucleic acid molecules of interest from a sample, (iii) clonally isolating the plurality of beads, (iv) cleaving the at least one primer, (v) clonally amplifying the nucleic acid thereby creating multiple amplification products, and (vi) analyzing the amplification products.
摘要翻译：本发明涉及用于分析感兴趣的多个核酸分子的方法，包括以下步骤：（i）提供多个珠粒，其特征在于每个珠粒包含至少两个序列特异性扩增引物，其特征还在于至少一个的引物通过可切割的接头与珠结合，（ii）从样品中捕获感兴趣的核酸分子，（iii）克隆分离多个珠，（iv）切割至少一个引物，（v）克隆扩增核酸从而产生多个扩增产物，和（vi）分析扩增产物。

5. 发明授权

US09347092B2 Solid support for high-throughput nucleic acid analysis 有权
标题翻译：坚固支持高通量核酸分析
公开(公告)号：US09347092B2
公开(公告)日：2016-05-24
申请号：US12705679
申请日：2010-02-15
申请人： Angelika Roesler , Thomas Froehlich , Dieter Heindl
发明人： Angelika Roesler , Thomas Froehlich , Dieter Heindl
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2523/319 , C12Q2525/197 , C12Q2563/149 , C12Q2565/537
摘要： The present invention provides a solid support which is preferably a bead comprising at least two sequence specific amplification primers wherein at least one primer is bound to the support with an inducible cleavable linker. The present invention also provides various method for preparing a solid support comprising at least two sequence specific primers, further characterized in that at least one of the primers is cleavable.
摘要翻译：本发明提供了一种固体支持物，其优选是包含至少两个序列特异性扩增引物的珠，其中至少一个引物用可诱导的可切割连接子与载体结合。本发明还提供了用于制备包含至少两个序列特异性引物的固体支持物的各种方法，其进一步的特征在于至少一种引物是可切割的。

6. 发明申请

US20100248991A1 SOLID SUPPORT FOR HIGH-THROUGHPUT NUCLEIC ACID ANALYSIS 有权
标题翻译：用于高通量核酸分析的固体支持
公开(公告)号：US20100248991A1
公开(公告)日：2010-09-30
申请号：US12705679
申请日：2010-02-15
申请人： Angelika Roesler , Thomas Froehlich , Dieter Heindl
发明人： Angelika Roesler , Thomas Froehlich , Dieter Heindl
IPC分类号： C40B40/06 , C07H21/00
CPC分类号： C12Q1/6844 , C12Q2523/319 , C12Q2525/197 , C12Q2563/149 , C12Q2565/537
摘要： The present invention provides a solid support which is preferably a bead comprising at least two sequence specific amplification primers wherein at least one primer is bound to the support with an inducible cleavable linker. The present invention also provides various method for preparing a solid support comprising at least two sequence specific primers, further characterized in that at least one of the primers is cleavable.
摘要翻译：本发明提供了一种固体支持物，其优选是包含至少两个序列特异性扩增引物的珠，其中至少一个引物用可诱导的可切割连接子与载体结合。本发明还提供了用于制备包含至少两个序列特异性引物的固体支持物的各种方法，其进一步的特征在于至少一种引物是可切割的。

7. 发明授权

US07759469B2 Labeling reagent 有权
标题翻译：标记试剂
公开(公告)号：US07759469B2
公开(公告)日：2010-07-20
申请号：US11370527
申请日：2006-03-08
申请人： Dieter Heindl , Thomas Froehlich , Heribert Maerz , Monika Seller
发明人： Dieter Heindl , Thomas Froehlich , Heribert Maerz , Monika Seller
IPC分类号： C07G3/00 , C07H21/00 , C12Q1/68
CPC分类号： C07H19/23 , C07H21/00
摘要： The current invention restates substituted nitroindole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted nitroindole nucleosides can be used as labeling reagents for the facile preparation of, e.g., optimized hybridization probes, simple probes, TAQMAN-probes or molecular beacon probes.
摘要翻译：本发明将取代的硝基吲哚核苷作为两个末端以及用于核酸检测，分析和定量的标记的寡核苷酸探针的内部构建块。取代基包括接头和可检测的基团或接头以及用于后合成偶联的反应性基团。这些修饰的核苷允许进入广泛的应用领域。这些新的取代的硝基吲哚核苷可以用作易于制备例如优化的杂交探针，简单探针，TAQMAN探针或分子信标探针的标记试剂。

8. 发明申请

US20060216736A1 Labeling reagent 有权
标题翻译：标记试剂
公开(公告)号：US20060216736A1
公开(公告)日：2006-09-28
申请号：US11370527
申请日：2006-03-08
申请人： Dieter Heindl , Thomas Froehlich , Heribert Maerz , Monika Seller
发明人： Dieter Heindl , Thomas Froehlich , Heribert Maerz , Monika Seller
IPC分类号： C12Q1/68 , C07H21/02 , C12P19/34 , C07H19/22
CPC分类号： C07H19/23 , C07H21/00
摘要： The current invention restates substituted nitroindole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted nitroindole nucleosides can be used as labeling reagents for the facile preparation of, e.g., optimized hybridization probes, simple probes, TAQMAN-probes or molecular beacon probes.
摘要翻译：本发明将取代的硝基吲哚核苷作为两个末端以及用于核酸检测，分析和定量的标记的寡核苷酸探针的内部构建块。取代基包括接头和可检测的基团或接头以及用于后合成偶联的反应性基团。这些修饰的核苷允许进入广泛的应用领域。这些新的取代的硝基吲哚核苷可以用作易于制备例如优化的杂交探针，简单探针，TAQMAN探针或分子信标探针的标记试剂。

9. 发明授权

US09650629B2 Clonal pre-amplification in emulsion 有权
公开(公告)号：US09650629B2
公开(公告)日：2017-05-16
申请号：US13169110
申请日：2011-06-27
申请人： Thomas Froehlich , Tobias Heckel
发明人： Thomas Froehlich , Tobias Heckel
IPC分类号： C12N15/10
CPC分类号： C12N15/1075 , C12N15/10 , C12N15/1096
摘要： Disclosed is a process for clonal pre-amplification of a nucleic acid involving the steps of (i) providing a plurality of different nucleic acid molecules (b) attaching adaptor sequences to the 3′ ends and 5′ ends of the nucleic acid molecules (c) preparing a water in oil emulsion wherein the majority of water droplets comprises one or none member of the plurality of different nucleic acid molecules (d) clonally amplifying the plurality of different nucleic acid molecules. In particular, the different nucleic acid molecules are mRNA molecules.

10. 发明授权

US08897460B2 Microphone amplifier 有权
标题翻译：麦克风放大器
公开(公告)号：US08897460B2
公开(公告)日：2014-11-25
申请号：US13330285
申请日：2011-12-19
申请人： Thomas Froehlich , Wolfgang Duenser
发明人： Thomas Froehlich , Wolfgang Duenser
IPC分类号： H04R3/00 , H03F1/52 , H03F3/187
CPC分类号： H03F3/187 , H03F1/523 , H03F2200/03 , H03F2200/78
摘要： A microphone amplifier comprises an input terminal (E100) for applying an input signal (IN), an output terminal (A100a) for outputting an output signal (OUT), and an additional output terminal (A100b) for outputting an additional output signal (VBIAS). The microphone amplifier also contains an amplifying circuit (10) for generating the output signal (OUT) by amplifying the input signal (IN), wherein the amplifying circuit (10) is connected between the input terminal (E100) and the output terminal (A100a), and a voltage generator (30) for generating the additional output signal (VBIAS). A supply voltage terminal (V30) of the voltage generator (30) is connected to the output terminal (A100a) of the microphone amplifier. Since the amplifying circuit (10) makes available a supply voltage (V) for the voltage generator (30), the microphone amplifier can be operated without a separate supply voltage terminal.
摘要翻译：麦克风放大器包括用于施加输入信号（IN）的输入端（E100），用于输出输出信号（OUT）的输出端（A100a）和用于输出附加输出信号（VBIAS）的附加输出端（A100b））。麦克风放大器还包括用于通过放大输入信号（IN）来产生输出信号（OUT）的放大电路（10），其中放大电路（10）连接在输入端（E100）和输出端（A100a ）和用于产生附加输出信号（VBIAS）的电压发生器（30）。电压发生器（30）的电源电压端子（V30）连接到麦克风放大器的输出端子（A100a）。由于放大电路（10）为电压发生器（30）提供电源电压（V），所以麦克风放大器可以在没有单独的电源电压端子的情况下工作。

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