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1. 发明授权

US07687260B2 Imaging fluorescence signals using telecentric optics 有权
标题翻译：使用远心光学成像荧光信号
公开(公告)号：US07687260B2
公开(公告)日：2010-03-30
申请号：US11333617
申请日：2006-01-17
申请人： Martin Gutekunst
发明人： Martin Gutekunst
IPC分类号： C12M1/34 , C12M3/00
CPC分类号： G01N21/6452 , G01N21/6456 , G01N21/6458 , G01N35/028 , G01N2021/6478 , G01N2035/00158
摘要： The present invention relates to the field of DNA analysis. In particular, the present invention is directed to a device for the parallel imaging of fluorescence intensities at a plurality of sites as a measure for DNA hybridization. More particular, the present invention is directed to a device to image multiplex real time PCR or to read out DNA microarrays.
摘要翻译：本发明涉及DNA分析领域。特别地，本发明涉及用于在多个位点处的荧光强度的平行成像的装置，作为DNA杂交的量度。更具体地，本发明涉及一种用于图像复用实时PCR或读出DNA微阵列的装置。

2. 发明授权

US08137616B2 System for multi color real time PCR 有权
标题翻译：多色实时PCR系统
公开(公告)号：US08137616B2
公开(公告)日：2012-03-20
申请号：US10549648
申请日：2004-04-01
申请人： Gregor Sagner , Ingrid Bechler , Jochen Bolte , Dieter Heindl , Hans-Peter Josel , Martin Gutekunst , Rudolf Sebl , Christoph Mueller
发明人： Gregor Sagner , Ingrid Bechler , Jochen Bolte , Dieter Heindl , Hans-Peter Josel , Martin Gutekunst , Rudolf Sebl , Christoph Mueller
IPC分类号： C12Q1/68 , G01N15/06 , G01N33/00 , G01N33/48 , G01N33/566 , C12P19/34
CPC分类号： C12Q1/6851 , G01N2021/6421 , C12Q2565/101 , C12Q2561/113 , C12Q2537/143
摘要： The invention is directed to a system for performing multi-color real time PCT, comprising a flexible real time PCT instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixture which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
摘要翻译：本发明涉及用于执行多色实时PCT的系统，其包括柔性实时PCT仪器和用于进行多重PCR的特定组合物或反应混合物：特别地，本发明涉及一种组合物或反应混合物，其中包含至少3个，优选4-5个，最优选正确的4对FRET杂交探针。每对所述杂交探针由携带FRET供体部分的FRET供体探针和携带具有550和710nm之间的发射最大值的FRET受体部分的FRET受体探针组成。

3. 发明授权

US08024132B2 Method for the efficiency-corrected real-time quantification of nucleic acids 有权
标题翻译：核酸的效率校正实时定量的方法
公开(公告)号：US08024132B2
公开(公告)日：2011-09-20
申请号：US12549150
申请日：2009-08-27
申请人： Gregor Sagner , Karim Tabiti , Martin Gutekunst , Richie Soong
发明人： Gregor Sagner , Karim Tabiti , Martin Gutekunst , Richie Soong
IPC分类号： G01N33/48 , G01N1/00
CPC分类号： G06F19/00 , C12Q1/6851 , C12Q2561/113 , C12Q2545/114 , C12Q2545/113
摘要： The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.
摘要翻译：本发明涉及用于定量样品中靶核酸的方法，包括以下步骤：（i）在确定的扩增条件下测定靶核酸的扩增效率，（ii）扩增含有的靶核酸在相同定义的反应条件下的样品中，（iii）实时测量扩增，（iv）通过用步骤（iii）得到的原始量的校正来定量样品中目标核酸的原始量，有助于确定的扩增效率。根据本发明的用于定量核酸的PCR反应的效率校正可以用于借助于外部或内部标准的绝对定量，以及与管家基因的表达相比进行相对定量。

4. 发明申请

US20060269922A1 System for multi color real time pcr 有权
标题翻译：多色实时系统
公开(公告)号：US20060269922A1
公开(公告)日：2006-11-30
申请号：US10549648
申请日：2004-04-01
申请人： Gregor Sagner , Ingrid Bechler , Jochen Bolte , Dieter Heindl , Hans-Peter Josel , Martin Gutekunst , Rudolf Sebl , Christoph Mueller
发明人： Gregor Sagner , Ingrid Bechler , Jochen Bolte , Dieter Heindl , Hans-Peter Josel , Martin Gutekunst , Rudolf Sebl , Christoph Mueller
IPC分类号： C12Q1/68 , C12P19/34 , C12M1/34 , C07H21/04
CPC分类号： C12Q1/6851 , G01N2021/6421 , C12Q2565/101 , C12Q2561/113 , C12Q2537/143
摘要： The invention is directed to a system for performing multi-color real time PCT, comprising a flexible real time PCT instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixtuer which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
摘要翻译：本发明涉及用于执行多色实时PCT的系统，其包括灵活的实时PCT仪器和用于进行多重PCR的特定组合物或反应混合物：特别地，本发明涉及一种组合物或反应混合器，其组合物或反应混合器包含至少3个，优选4-5个，最优选正确的4对FRET杂交探针。每对所述杂交探针由携带FRET供体部分的FRET供体探针和携带具有550和710nm之间的发射最大值的FRET受体部分的FRET受体探针组成。

5. 发明授权

US06503720B2 Method for quantification of an analyte 有权
公开(公告)号：US06503720B2
公开(公告)日：2003-01-07
申请号：US09789170
申请日：2001-02-20
申请人： Carl T. Wittwer , Martin Gutekunst , Sabine Lohmann
发明人： Carl T. Wittwer , Martin Gutekunst , Sabine Lohmann
IPC分类号： C12Q168
CPC分类号： C12Q1/6851 , C12Q2537/165
摘要： Methods are provided for quantifying the concentration of a nucleic acid in a nucleic acid sample. The methods include contacting the nucleic acid sample with an amplifying agent, amplifying at least one predetermined locus of the nucleic acid by subjecting the sample to a number of amplification, generating an amplification curve or array, calculating the first, second or n th order derivative of the amplification curve or array, determining a maximum, minimum, or zero value of the derivative, and using the maximum, minimum, or zero value to calculate the initial concentration of the nucleic acid in the nucleic acid sample.

6. 发明授权

US06303305B1 Method for quantification of an analyte 有权
标题翻译：分析物定量方法
公开(公告)号：US06303305B1
公开(公告)日：2001-10-16
申请号：US09281448
申请日：1999-03-30
申请人： Carl T. Wittwer , Martin Gutekunst , Sabine Lohmann
发明人： Carl T. Wittwer , Martin Gutekunst , Sabine Lohmann
IPC分类号： C12Q168
CPC分类号： C12Q1/6851 , C12Q2537/165
摘要： The invention provides a new method for the quantification of a nucleic acid, wherein in a first step, the nucleic acid is amplified by an amplifying agent. Subsequently, first, second or n th order derivatives is calculated. The obtained value can then be used to calculate the initial concentration of the analyte.
摘要翻译：本发明提供了一种用于定量核酸的新方法，其中在第一步中，核酸通过扩增剂扩增。随后，计算第一，第二或第n阶导数。然后可以使用获得的值来计算分析物的初始浓度。

7. 发明申请

US20080248478A1 MOLECULAR HISTOLOGICAL ANALYSIS OF MULTICELLULAR SAMPLES 审中-公开
标题翻译：分子样品的分子生物学分析
公开(公告)号：US20080248478A1
公开(公告)日：2008-10-09
申请号：US12020619
申请日：2008-01-28
申请人： Jochen Renzing , Roderic Fuerst , Martin Gutekunst
发明人： Jochen Renzing , Roderic Fuerst , Martin Gutekunst
IPC分类号： C12Q1/68 , C12M1/00 , G01N33/53 , G06K9/78 , C12Q1/02
CPC分类号： G01N33/6803 , G06K9/0014
摘要： The present invention provides modified digital images of a multicellular sample using molecular profiles of cells from said multicellular sample for a molecular histological analysis.
摘要翻译：本发明使用来自所述多细胞样品的细胞的分子谱进行分子组织学分析来提供多细胞样品的修饰数字图像。

8. 发明授权

US07369227B2 Imaging fluorescence signals using telecentricity 有权
标题翻译：使用远心成像荧光信号
公开(公告)号：US07369227B2
公开(公告)日：2008-05-06
申请号：US11333390
申请日：2006-01-17
申请人： Martin Gutekunst , Frank Guse , Hans-Georg Heckmann , Martin Hessling , Joachim Koenig
发明人： Martin Gutekunst , Frank Guse , Hans-Georg Heckmann , Martin Hessling , Joachim Koenig
IPC分类号： G01J3/30
CPC分类号： G01N21/6456 , G01J3/02 , G01J3/0208 , G01J3/021 , G01J3/4406 , G01N21/6428 , G01N21/6452 , G01N35/028 , G01N2021/6478 , G01N2035/00158
摘要： The present invention relates to the field of DNA analysis. In particular, the present invention is directed to a device for the parallel imaging of fluorescence intensities at a plurality of sites as a measure for DNA hybridization. More particular, the present invention is directed to a device to image multiplex real time PCR or to read out DNA microarrays.
摘要翻译：本发明涉及DNA分析领域。特别地，本发明涉及用于在多个位点处的荧光强度的平行成像的装置，作为DNA杂交的量度。更具体地，本发明涉及一种用于图像复用实时PCR或读出DNA微阵列的装置。

9. 发明申请

US20060194308A1 Imaging fluorescence signals using telecentricity 有权
标题翻译：使用远心成像荧光信号
公开(公告)号：US20060194308A1
公开(公告)日：2006-08-31
申请号：US11333390
申请日：2006-01-17
申请人： Martin Gutekunst , Frank Guse , Hans-Georg Heckmann , Martin Hessling , Joachim Koenig
发明人： Martin Gutekunst , Frank Guse , Hans-Georg Heckmann , Martin Hessling , Joachim Koenig
IPC分类号： C12M1/34 , C12M3/00
CPC分类号： G01N21/6456 , G01J3/02 , G01J3/0208 , G01J3/021 , G01J3/4406 , G01N21/6428 , G01N21/6452 , G01N35/028 , G01N2021/6478 , G01N2035/00158
摘要： The present invention relates to the field of DNA analysis. In particular, the present invention is directed to a device for the parallel imaging of fluorescence intensities at a plurality of sites as a measure for DNA hybridization. More particular, the present invention is directed to a device to image multiplex real time PCR or to read out DNA microarrays.
摘要翻译：本发明涉及DNA分析领域。特别地，本发明涉及用于在多个位点处的荧光强度的平行成像的装置，作为DNA杂交的量度。更具体地，本发明涉及一种用于图像复用实时PCR或读出DNA微阵列的装置。

10. 发明授权

US06691041B2 Method for the efficiency-corrected real-time quantification of nucleic acids 有权
标题翻译：核酸的效率校正实时定量的方法
公开(公告)号：US06691041B2
公开(公告)日：2004-02-10
申请号：US09823711
申请日：2001-03-30
申请人： Gregor Sagner , Karim Tabiti , Martin Gutekunst , Richie Soong
发明人： Gregor Sagner , Karim Tabiti , Martin Gutekunst , Richie Soong
IPC分类号： G01N3348
CPC分类号： G06F19/00 , C12Q1/6851 , C12Q2561/113 , C12Q2545/114 , C12Q2545/113
摘要： The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.
摘要翻译：本发明涉及用于定量样品中靶核酸的方法，包括以下步骤：（i）在确定的扩增条件下测定靶核酸的扩增效率，（ii）扩增含有的靶核酸在相同定义的反应条件下的样品中，（iii）实时测量扩增，（iv）通过用步骤（iii）得到的原始量的校正来定量样品中目标核酸的原始量，有助于确定的扩增效率。根据本发明的用于定量核酸的PCR反应的效率校正可以用于借助于外部或内部标准的绝对定量，以及与管家基因的表达相比进行相对定量。

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