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1. 发明授权

US5654180A Hybrid plasmid vectors, recombinant plasmids containing genes encoding nitrile degrading enzymes, transformants containing the recombinant plasmids and methods of producing amides and acids using the transformants 失效
标题翻译：杂交质粒载体，含有编码腈降解酶的基因的重组质粒，含有重组质粒的转化体和使用转化体产生酰胺和酸的方法
公开(公告)号：US5654180A
公开(公告)日：1997-08-05
申请号：US344683
申请日：1994-11-17
申请人： Teruhiko Beppu , Sueharu Horinouchi , Makoto Nishiyama , Fujio Yu , Yoshihiro Hashimoto
发明人： Teruhiko Beppu , Sueharu Horinouchi , Makoto Nishiyama , Fujio Yu , Yoshihiro Hashimoto
IPC分类号： C12N1/21 , C12N9/78 , C12N15/55 , C12N15/74 , C12P13/02 , C12N1/20 , C12P7/40
CPC分类号： C12N15/74 , C12N9/78
摘要： The present invention provides a recombinant plasmid comprising combining a hybrid plasmid vector with the isolated DNA sequences of one or more genes encoding nitrile degrading enzymes which are derived from bacteria belonging to the genus Rhodococcus, said hybrid plasmid vector comprising an isolated DNA sequence which confers on the vector the ability to replicate and amplify in the cells of bacteria belonging to the genus Rhodococcus, and an isolated DNA sequence which confers on the vector the ability to replicate and amplify in the cells of bacteria belonging to Escherichia coli, and an isolated DNA sequence containing a drug resistance gene.
摘要翻译：本发明提供了一种重组质粒，其包含将杂交质粒载体与编码来自属于红球菌属的细菌的腈降解酶的一个或多个基因的分离的DNA序列组合，所述杂交质粒载体包含赋予的分离的DNA序列载体能够在属于红球菌属的细菌的细胞中复制和扩增的能力，以及赋予载体在分离的DNA序列的细胞中复制和扩增能力的分离的DNA序列含有耐药性基因。

2. 发明授权

US5246857A Circular plasmids derived from the genus rhodococcus 失效
标题翻译：来自基因RHODOCOCCUS的循环血浆
公开(公告)号：US5246857A
公开(公告)日：1993-09-21
申请号：US773055
申请日：1991-10-08
申请人： Fujio Yu , Yoshihiro Hashimoto
发明人： Fujio Yu , Yoshihiro Hashimoto
IPC分类号： C12N15/74
CPC分类号： C12N15/74
摘要： The present invention provides an isolated circular plasmid of about 2.6 kb derived from a bacterium belonging to the genus Rhodococcus whose restriction sites compises two SacI sites, one BamII site, one PvuI site, one ScaI site, one SphI site and one XhoI site. In addition, the present invention provides an isolated circular plasmid of about 7.6 kb derived from a bacterium belonging to the genus Rhodococcus whose restriction sites compises one SphI site, two KpnI sites, one BglII site, and three SacI sites. The vectors of the invention can be suitable for Rhodococcus hosts and usefull in industry.

3. 发明授权

US07557202B2 DNA fragments containing gene having function relating to autonomous proliferation of plasmid 有权
标题翻译：含有与质粒自主增殖有关的功能基因的DNA片段
公开(公告)号：US07557202B2
公开(公告)日：2009-07-07
申请号：US10482156
申请日：2002-07-03
申请人： Wataru Mizunashi , Fujio Yu
发明人： Wataru Mizunashi , Fujio Yu
IPC分类号： C07H21/04 , C12N1/20 , C12N15/00
CPC分类号： C07K14/195 , C12N15/69
摘要： Provided are a DNA fragment containing a gene relating the autonomous replication of a plasmid, and a DNA fragment containing a gene relating to the autonomous replication of a plasmid wherein a mutation point capable of increasing the number of copies of a plasmid is present in at least one site in the gene. Also provided are a DNA fragment containing a gene having a function relating to the autonomous replication of a plasmid within the bacteria of the genus Rhodococcus, a DNA fragment wherein a mutation point capable of increasing the number of copies of a plasmid is present in at least one site in the gene, and a plasmid carrying a DNA fragment wherein a mutation point capable of increasing the number of copies of a plasmid is present in at least one site of the gene.
摘要翻译：提供了含有与质粒自主复制有关的基因的DNA片段和含有与质粒自主复制有关的基因的DNA片段，其中能够增加质粒拷贝数的突变点至少存在基因中的一个位点。还提供了含有与红球菌属细菌中的质粒自主复制有关的功能的基因的DNA片段，其中至少存在能增加质粒拷贝数的突变点的DNA片段该基因的一个位点和携带DNA片段的质粒，其中能够增加质粒拷贝数的突变点存在于该基因的至少一个位点。

4. 发明授权

US5776771A Kanamycin resistance gene derived from microorganisms of the genus rhodococcus 失效
标题翻译：源自红球菌属微生物的卡那霉素抗性基因
公开(公告)号：US5776771A
公开(公告)日：1998-07-07
申请号：US510878
申请日：1995-08-03
申请人： Fujio Yu , Mami Kato
发明人： Fujio Yu , Mami Kato
IPC分类号： C12N15/09 , C07K14/195 , C07K14/41 , C12N1/21 , C12N9/00 , C12N15/65 , C12N15/74 , C12P21/02 , C12R1/01 , C12R1/19 , C12N15/31 , C12N15/70
CPC分类号： C12N15/65 , C07K14/195 , C12N15/74 , C12N9/00
摘要： The present invention relates to a DNA derived from microorganisms of the genus Rhodococcus and conferring kanamycin resistance on hosts with a DNA sequence coding for the amino acid sequence of Sequence No. 1 or a polypeptide containing a partial sequence thereof. The kanamycin resistance gene of the present invention is useful to construct vectors for microorganisms of the genus Rhodococcus, particularly vectors for self-cloning of Rhodococcus rhodochrous.
摘要翻译：本发明涉及一种衍生自红球菌属微生物的DNA，并赋予具有编码序列号1的氨基酸序列的DNA序列的宿主的卡那霉素抗性或含有其部分序列的多肽。本发明的卡那霉素抗性基因可用于构建红球菌属微生物的载体，特别是用于玫瑰色红球菌自身克隆的载体。

5. 发明授权

US5602014A Regulatory system for expression of nitrilase gene 失效
标题翻译：腈水解酶基因表达调控系统
公开(公告)号：US5602014A
公开(公告)日：1997-02-11
申请号：US577184
申请日：1995-12-22
申请人： Yurie Mizumura , Fujio Yu
发明人： Yurie Mizumura , Fujio Yu
IPC分类号： C12N15/09 , C07K14/195 , C12N1/21 , C12N9/78 , C12N15/31 , C12N15/74 , C12R1/01 , C12N15/00 , C12P21/00
CPC分类号： C12N9/78 , C07K14/195 , C12N15/74
摘要： The invention relates to a regulatory factor or system composed of 2 components of a polypeptide having the amino acid sequence of SEQ ID No:1 and a polypeptide having the amino acid sequence of SEQ ID No:2 to activate a nitrilase gene promoter, as well as to DNA coding therefor. Nitrilase can be produced by introducing the gene coding for the present regulatory factor together with a nitrilase gene containing a promoter region into a microorganism of the genus Rhodococcus.
摘要翻译：本发明涉及由具有SEQ ID No：1的氨基酸序列的多肽的2种成分和具有SEQ ID No：2的氨基酸序列的多肽组成的调节因子或系统，以激活腈水解酶基因启动子关于DNA编码。可以通过将编码本调节因子的基因与含有启动子区域的腈水解酶基因导入红球菌属的微生物来生产腈水解酶。

6. 发明授权

US08383389B2 Hydroxynitrile lyase 失效
公开(公告)号：US08383389B2
公开(公告)日：2013-02-26
申请号：US13210646
申请日：2011-08-16
申请人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
发明人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
IPC分类号： C12N9/00 , C12N9/88 , C12N1/20 , C12N15/00
CPC分类号： C12N9/88 , C12P7/42 , C12P13/004
摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.

7. 发明申请

US20100136617A1 HALOHYDRIN EPOXIDASE 失效
标题翻译：盐酸环氧化酶
公开(公告)号：US20100136617A1
公开(公告)日：2010-06-03
申请号：US12529657
申请日：2008-03-07
申请人： Takanori Akiyama , Fujio Yu , Fumiaki Watanabe , Yuya Takikawa , Eiji Sato
发明人： Takanori Akiyama , Fujio Yu , Fumiaki Watanabe , Yuya Takikawa , Eiji Sato
IPC分类号： C12P21/06 , C12N9/88 , C07H21/04 , C12N15/63 , C12N5/10 , C12P17/02 , C12P13/00
CPC分类号： C12P13/004 , C12N9/0071
摘要： The present invention provides an industrially useful improved halohydrin epoxidase, a method for producing the same, and a method for producing an epihalohydrin or 4-halo-3-hydroxybutyronitrile using the same. The improved halohydrin epoxidase of the present invention consists of an amino acid sequence in which a specific amino acid substitution mutation is introduced into an amino acid sequence of a wild-type halohydrin epoxidase comprising predetermined amino acid sequences I and II.
摘要翻译：本发明提供工业上有用的改进的卤代醇环氧酶，其制备方法，以及使用其制备表卤代醇或4-卤代-3-羟基丁腈的方法。本发明的改进的卤代醇环氧酶由将特定氨基酸取代突变引入包含预定氨基酸序列I和II的野生型卤代醇环氧酶的氨基酸序列中的氨基酸序列组成。

8. 发明申请

US20060252086A1 Process for producing vinylated nucleic acid 审中-公开
标题翻译：生产乙烯基化核酸的方法
公开(公告)号：US20060252086A1
公开(公告)日：2006-11-09
申请号：US11438463
申请日：2006-05-23
申请人： Toshitaka Uragaki , Fumiaki Watanabe , Chiaki Nagahama , Fujio Yu , Katsuaki Kikuchi , Akira Yoshioka
发明人： Toshitaka Uragaki , Fumiaki Watanabe , Chiaki Nagahama , Fujio Yu , Katsuaki Kikuchi , Akira Yoshioka
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C07H21/00
摘要： This invention provides a process for producing a vinylated nucleic acid in an efficient and cost-effective manner through the implementation of vinylation of an amino-containing nucleic acid with a vinylating agent. This invention also provides a process for producing a vinylated nucleic acid in an efficient and cost-effective manner by a method in which PCR is carried out in the presence of an amino-modified nucleotide and dNTP and a vinyl group is introduced into the resulting PCR amplification product or by a method in which PCR is carried out in the presence of a vinyl-modified nucleotide and cNTP.
摘要翻译：本发明提供了一种通过使用乙烯化剂实施含氨基核酸的乙烯化作用，以有效且成本有效的方式生产乙烯基化核酸的方法。本发明还提供了通过在氨基修饰的核苷酸和dNTP的存在下进行PCR并将乙烯基引入到所得PCR中的方法，以有效和成本有效的方式制备乙烯基化核酸的方法扩增产物或通过在乙烯基修饰的核苷酸和cNTP存在下进行PCR的方法。

9. 发明授权

US5705386A Circular plasmids 失效
标题翻译：圆形质粒
公开(公告)号：US5705386A
公开(公告)日：1998-01-06
申请号：US678650
申请日：1996-07-11
申请人： Wataru Mizunashi , Fujio Yu
发明人： Wataru Mizunashi , Fujio Yu
IPC分类号： C12N15/09 , C07H21/04 , C12N15/74 , C12R1/01 , C12N15/63
CPC分类号： C12N15/74
摘要： The specification relates to circular plasmids derived from microorganisms of the genus Rhodococcus, which have sizes of about 3.6 kb and restriction site numbers of BamHI:1, BglII:1, ClaI:1, PstI:1, PvuII:2 and SacI:1. The circular plasmids of the present invention are useful as vectors in industrially useful host-vector systems.
摘要翻译：本说明书涉及衍生自红球菌属微生物的圆形质粒，其具有约3.6kb的大小和BamHI：1，BglII：1，ClaI：1，PstI：1，PvuII：2和SacI：1的限制性位点编号。本发明的环状质粒可用作工业上有用的宿主 - 载体系统中的载体。

10. 发明授权

US5210031A Process for the production of R(-)-4-halo-3-hydroxybutyronitrile 失效
标题翻译：制备R（ - ） - 4-卤代-3-羟基丁腈的方法
公开(公告)号：US5210031A
公开(公告)日：1993-05-11
申请号：US830516
申请日：1992-02-03
申请人： Tetsuji Nakamura , Fujio Yu
发明人： Tetsuji Nakamura , Fujio Yu
IPC分类号： C12P13/00
CPC分类号： C12P13/002 , Y10S435/822 , Y10S435/843 , Y10S435/844 , Y10S435/845 , Y10S435/846
摘要： A process for the production of a 4-halo-3-hydroxybutyronitrile which comprises reacting an epihalohydrin with a halohydrin-halide-lyase originating from a microorganism selected from the group consisting of: Corynebacterium sp. N-2354 FERM BP-2726 and Microbacterium sp. N-4701 FERM BP-2644 in the presence of an alkali cyanide to thereby convert the epihalohydrin into the 4-halo-3-hydroxybutyronitrile and collecting the product thus formed is disclosed. According to this process, a 4-halo-3-hydroxybutyronitrile which is highly useful in the syntheses of various medicines and physiologically active substances can be easily and efficiently produced from an inexpensive starting material.
摘要翻译：一种制备4-卤代-3-羟基丁腈的方法，其包括使表卤代醇与源自选自以下的微生物的卤代醇 - 卤化物裂解酶反应：棒状杆菌属 N-2354 FERM BP-2726和Microbacterium sp。 N-4701 FERM BP-2644在碱金属氰化物的存在下，从而将表卤代醇转化成4-卤代-3-羟基丁腈并收集如此形成的产品。根据该方法，可以从廉价的原料容易且有效地制备在各种药物和生理活性物质的合成中非常有用的4-卤代-3-羟基丁腈。

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