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1. 发明授权

US08530208B2 Method for producing phospholipid 有权
标题翻译：磷脂生产方法
公开(公告)号：US08530208B2
公开(公告)日：2013-09-10
申请号：US13382815
申请日：2010-07-05
申请人： Tatsushi Tanaka , Yugo Iwasaki
发明人： Tatsushi Tanaka , Yugo Iwasaki
IPC分类号： C12P7/62
CPC分类号： C11C3/10 , A23D9/013 , C12P7/6472 , C12P7/6481
摘要： Provided is a method for producing a phospholipid at low cost by reusing phospholipase A2 in a method for producing the phospholipid whereby an arbitrary fatty acid is bonded to the 2-position of a phospholipid using an esterification reaction catalyzed by phospholipase A2 in glycerol. The method for producing a phospholipid is characterized by comprising conducting an esterification reaction catalyzed by phospholipase A2 between a lysophospholipid and an acyl donor in glycerol to from a phospholipid, adding a solvent immiscible with glycerol to form a glycerol layer and a solvent layer, extracting said phospholipid into said solvent layer, allowing phospholipase A2 to migrate into said glycerol layer, and, after separating the glycerol layer and distilling off the solvent remaining therein, further adding to the residual glycerol solution the lysophospholipid and the acyl donor to thereby conduct the esterification reaction again with use of phospholipase A2 remaining in said glycerol solution.
摘要翻译：本发明提供一种在磷脂生产方法中使用磷脂酶A2的方法，通过在磷脂中磷脂酶A2催化的酯化反应使任意的脂肪酸与磷脂的2位结合，从而低成本地制造磷脂。磷脂的制造方法的特征在于，在磷脂中，通过磷脂酶A2在溶血磷脂和酰基供体之间进行由磷脂催化的酯化反应，加入与甘油不混溶的溶剂，形成甘油层和溶剂层，提取所述磷脂进入所述溶剂层，使磷脂酶A2迁移到所述甘油层中，并且在分离甘油层并蒸除剩余的溶剂后，进一步向残留的甘油溶液中加入溶血磷脂和酰基供体，从而进行酯化反应再次使用残留在所述甘油溶液中的磷脂酶A2。

2. 发明申请

US20120100580A1 METHOD FOR PRODUCING PHOSPHOLIPID 有权
标题翻译：生产磷脂的方法
公开(公告)号：US20120100580A1
公开(公告)日：2012-04-26
申请号：US13382815
申请日：2010-07-05
申请人： Tatsushi Tanaka , Yugo Iwasaki
发明人： Tatsushi Tanaka , Yugo Iwasaki
IPC分类号： C12P13/00
CPC分类号： C11C3/10 , A23D9/013 , C12P7/6472 , C12P7/6481
摘要： Provided is a method for producing a phospholipid at low cost by reusing phospholipase A2 in a method for producing the phospholipid whereby an arbitrary fatty acid is bonded to the 2-position of a phospholipid using an esterification reaction catalyzed by phospholipase A2 in glycerol. The method for producing a phospholipid is characterized by comprising conducting an esterification reaction catalyzed by phospholipase A2 between a lysophospholipid and an acyl donor in glycerol to from a phospholipid, adding a solvent immiscible with glycerol to form a glycerol layer and a solvent layer, extracting said phospholipid into said solvent layer, allowing phospholipase A2 to migrate into said glycerol layer, and, after separating the glycerol layer and distilling off the solvent remaining therein, further adding to the residual glycerol solution the lysophospholipid and the acyl donor to thereby conduct the esterification reaction again with use of phospholipase A2 remaining in said glycerol solution.
摘要翻译：本发明提供一种在磷脂生产方法中使用磷脂酶A2的方法，通过在磷脂中磷脂酶A2催化的酯化反应使任意的脂肪酸与磷脂的2位结合，从而低成本地制造磷脂。磷脂的制造方法的特征在于，在磷脂中，通过磷脂酶A2在溶血磷脂和酰基供体之间进行由磷脂催化的酯化反应，加入与甘油不混溶的溶剂，形成甘油层和溶剂层，提取所述磷脂进入所述溶剂层，使磷脂酶A2迁移到所述甘油层中，并且在分离甘油层并蒸除剩余的溶剂后，进一步向残留的甘油溶液中加入溶血磷脂和酰基供体，从而进行酯化反应再次使用残留在所述甘油溶液中的磷脂酶A2。

3. 发明申请

US20050250189A1 Process for recovering serine 审中-公开
标题翻译：回收丝氨酸的方法
公开(公告)号：US20050250189A1
公开(公告)日：2005-11-10
申请号：US11117489
申请日：2005-04-29
申请人： Tsuneo Yamane , Yugo Iwasaki , Namiko Takahashi , Takaya Yamamoto , Takahiro Okada
发明人： Tsuneo Yamane , Yugo Iwasaki , Namiko Takahashi , Takaya Yamamoto , Takahiro Okada
IPC分类号： C12P13/06 , C07F9/10 , C12P13/04
CPC分类号： C07F9/10
摘要： Particularly in the recovery and reuse of unreacted serine after the synthesis of phosphatidylserine by the transfer reaction of a phosphatidyl group with phospholipase D, a simple process for recovering serine in which a material inhibitory to the synthesis of phosphatidylserine can be efficiently removed is provided. The present invention provides the following processes for recovering serine. A process for recovering unreacted serine from a reaction system after the synthesis of phosphatidylserine by the transfer reaction of a phosphatidyl group with phospholipase D, comprising adding a polar organic solvent or a mixture of a polar organic solvent and water to the unreacted serine containing water-soluble fraction in which the phosphatidylserine has been removed from a reaction mixture in the reaction system and washing the fraction with the added solvent or the mixture of the solvent and water to obtain the serine. A process for recovering the serine described above, wherein the synthesis of phosphatidylserine by the transfer reaction of a phosphatidyl group with phospholipase D is performed in an aqueous system in the absence of an organic solvent. A process for recovering the serine described above, wherein the synthesis of phosphatidylserine by the transfer reaction of a phosphatidyl group with phospholipase D is performed in a two-phase system with an organic solvent and water.
摘要翻译：特别是在通过磷脂酰基与磷脂酶D的转移反应合成磷脂酰丝氨酸后的未反应丝氨酸的回收和再利用中，提供了一种简单的回收丝氨酸的方法，其中可以有效地除去抑制磷脂酰丝氨酸合成的材料。本发明提供以下回收丝氨酸的方法。通过磷脂酰基与磷脂酶D的转移反应合成磷脂酰丝氨酸后，从反应体系中回收未反应的丝氨酸的方法，其包括将极性有机溶剂或极性有机溶剂与水的混合物添加到含有水溶性聚合物的未反应的丝氨酸中，其中已经从反应体系中的反应混合物中除去磷脂酰丝氨酸并用添加的溶剂或溶剂和水的混合物洗涤级分以获得丝氨酸的可溶性级分。一种回收上述丝氨酸的方法，其中通过磷脂酰基与磷脂酶D的转移反应合成磷脂酰丝氨酸是在不存在有机溶剂的情况下在水性体系中进行的。一种回收上述丝氨酸的方法，其中通过磷脂酰基与磷脂酶D的转移反应合成磷脂酰丝氨酸在具有有机溶剂和水的两相体系中进行。

4. 发明授权

US06660504B2 Process for exchanging bases in phospholipids 有权
标题翻译：在磷脂中交换碱基的方法
公开(公告)号：US06660504B2
公开(公告)日：2003-12-09
申请号：US10042280
申请日：2002-01-11
申请人： Tsuneo Yamane , Yugo Iwasaki , Yukiko Mizumoto , Masaaki Kasai , Takahiro Okada
发明人： Tsuneo Yamane , Yugo Iwasaki , Yukiko Mizumoto , Masaaki Kasai , Takahiro Okada
IPC分类号： C12N916
CPC分类号： C12P7/6481 , C12P13/00 , C12P13/06
摘要： The present invention provides a process for preparing a phospholipid in an aqueous system in which hydrolysis is extremely controlled, and the synthetic yield is improved. A process for exchanging a base of a phospholipid as a raw material by subjecting the phospholipid to the action of phospholipase D in the presence of a receptor having a hydroxyl group, in which the reaction is carried out in an aqueous system, a phospholipid adsorbed on a carrier is used as a raw material phospholipid, and the receptor and the phospholipase D are used in free forms.
摘要翻译：本发明提供了一种在水性体系中制备磷脂的方法，其中水解被极度控制，并且合成产率提高。1.一种通过使磷脂作用磷脂酶作为原料来交换磷脂的碱的方法在具有羟基的受体的存在下，其中反应在水性体系中进行，吸附在载体上的磷脂用作原料磷脂，受体和磷脂酶D以游离形式使用。

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