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1. 发明授权

US08362449B2 Total internal reflection microscope apparatus and method for analyzing fluorescent sample 有权
标题翻译：全内反射显微镜装置及荧光试样分析方法
公开(公告)号：US08362449B2
公开(公告)日：2013-01-29
申请号：US13147386
申请日：2009-11-30
申请人： Takuya Matsui , Satoshi Takahashi , Takanobu Haga
发明人： Takuya Matsui , Satoshi Takahashi , Takanobu Haga
IPC分类号： G01N21/64 , G01N21/01
CPC分类号： G01N21/6458 , G01N21/648 , G01N2021/6432 , G02B21/16
摘要： An object of the present invention relates to observation of single molecule fluorescence while temperature of a sample solution is controlled by a temperature controller and intrinsic fluorescence of the temperature controller is avoided, in a total internal reflection microscope. The present invention relates to provision of an opening at areas of the temperature controller through which incident light and reflected light pass, and configuration adopting a material with intrinsic fluorescence lower than that of the other parts, in a total internal reflection microscope including a prism and the temperature controller. The present invention enables intrinsic fluorescence of the temperature controller to be suppressed, which allows highly sensitive fluorescence observation while controlling sample solution temperature with high precision. For instance, this in turn allows the throughput of single molecule DNA sequencing using a total internal reflection microscope to be improved.
摘要翻译：本发明的目的在于，在全内反射显微镜中，通过温度控制器控制样品溶液的温度并避免温度控制器的固有荧光，从而观察单分子荧光。本发明涉及在温度控制器的入射光和反射光通过的区域，以及采用具有比其他部分低的固有荧光的材料的结构，在包括棱镜的全内反射显微镜中提供开口，温度控制器。本发明能够抑制温度控制器的固有荧光，从而高精度地控制样品溶液温度，从而实现高灵敏度的荧光观察。例如，这又允许改进使用全内反射显微镜的单分子DNA测序的生产量。

2. 发明授权

US08389959B2 Fluorescence analyzing device and fluorescence analyzing method 有权
标题翻译：荧光分析装置及荧光分析方法
公开(公告)号：US08389959B2
公开(公告)日：2013-03-05
申请号：US13147147
申请日：2009-11-30
申请人： Satoshi Takahashi , Tsuyoshi Sonehara , Tomoyuki Sakai , Takanobu Haga , Hirokazu Kato , Nobutaka Kumazaki , Takuya Matsui
发明人： Satoshi Takahashi , Tsuyoshi Sonehara , Tomoyuki Sakai , Takanobu Haga , Hirokazu Kato , Nobutaka Kumazaki , Takuya Matsui
IPC分类号： G01N21/64
CPC分类号： G01N21/648 , G01J3/10 , G01J3/14 , G01J3/36 , G01J3/4406 , G01N21/6452
摘要： The present invention has an object to provide a method for efficiently detecting an image with a smaller number of pixels.The invention relates to fluorescence analysis which uses a substrate having a plurality of regions for being capable of immobilizing biologically-related molecules in positions of lattice points of a lattice structure, and which causes the fluorescence from a certain lattice point to be wavelength-dispersed in a direction other than the direction toward the adjacent closest lattice point. According to an embodiment, for example, the number of pixels of a two-dimensional sensor required for fluorescence analysis of the regions with the biologically-related molecules immobilized can be set to several hundred times to fifty times smaller than that in the conventional case without degrading the measurement accuracy. This can achieve the improvement of throughput, reduction in price, and/or improvement of the operability of an analyzing device.
摘要翻译：本发明的目的是提供一种用于有效检测具有较少像素数的图像的方法。本发明涉及使用具有多个区域的基板的荧光分析，其能够将生物相关分子固定在格子结构的格点的位置，并且使得来自特定格子点的荧光波长分散在除了朝向相邻最近格子点的方向之外的方向。根据一个实施例，例如，具有生物相关分子固定的区域的荧光分析所需的二维传感器的像素数量可以设定为比常规情况下少几百次到五十次，而没有降低测量精度。这可以实现分析装置的吞吐量的提高，价格的降低和/或改善可操作性。

3. 发明申请

US20110284768A1 FLUORESCENCE ANALYZING DEVICE AND FLUORESCENCE ANALYZING METHOD 有权
标题翻译：荧光分析装置和荧光分析方法
公开(公告)号：US20110284768A1
公开(公告)日：2011-11-24
申请号：US13147147
申请日：2009-11-30
申请人： Satoshi Takahashi , Tsuyoshi Sonehara , Tomoyuki Sakai , Takanobu Haga , Hirokazu Kato , Nobutaka Kumazaki , Takuya Matsui
发明人： Satoshi Takahashi , Tsuyoshi Sonehara , Tomoyuki Sakai , Takanobu Haga , Hirokazu Kato , Nobutaka Kumazaki , Takuya Matsui
IPC分类号： G01N21/64
CPC分类号： G01N21/648 , G01J3/10 , G01J3/14 , G01J3/36 , G01J3/4406 , G01N21/6452
摘要： The present invention has an object to provide a method for efficiently detecting an image with a smaller number of pixels.The invention relates to fluorescence analysis which uses a substrate having a plurality of regions for being capable of immobilizing biologically-related molecules in positions of lattice points of a lattice structure, and which causes the fluorescence from a certain lattice point to be wavelength-dispersed in a direction other than the direction toward the adjacent closest lattice point. According to an embodiment, for example, the number of pixels of a two-dimensional sensor required for fluorescence analysis of the regions with the biologically-related molecules immobilized can be set to several hundred times to fifty times smaller than that in the conventional case without degrading the measurement accuracy. This can achieve the improvement of throughput, reduction in price, and/or improvement of the operability of an analyzing device.
摘要翻译：本发明的目的是提供一种用于有效检测具有较少像素数的图像的方法。本发明涉及使用具有多个区域的基板的荧光分析，其能够将生物相关分子固定在格子结构的格点的位置，并且使得来自特定格子点的荧光波长分散在除了朝向相邻最近格子点的方向之外的方向。根据一个实施例，例如，具有生物相关分子固定的区域的荧光分析所需的二维传感器的像素数量可以设定为比常规情况下少几百次到五十次，而没有降低测量精度。这可以实现分析装置的吞吐量的提高，价格的降低和/或改善可操作性。

4. 发明申请

US20110284769A1 TOTAL INTERNAL REFLECTION MICROSCOPE APPARATUS AND METHOD FOR ANALYZING FLUORESCENT SAMPLE 有权
标题翻译：总内反射显微镜装置和分析荧光体样品的方法
公开(公告)号：US20110284769A1
公开(公告)日：2011-11-24
申请号：US13147386
申请日：2009-11-30
申请人： Takuya Matsui , Satoshi Takahashi , Takanobu Haga
发明人： Takuya Matsui , Satoshi Takahashi , Takanobu Haga
IPC分类号： G01N21/64 , G01N21/01
CPC分类号： G01N21/6458 , G01N21/648 , G01N2021/6432 , G02B21/16
摘要： An object of the present invention relates to observation of single molecule fluorescence while temperature of a sample solution is controlled by a temperature controller and intrinsic fluorescence of the temperature controller is avoided, in a total internal reflection microscope. The present invention relates to provision of an opening at areas of the temperature controller through which incident light and reflected light pass, and configuration adopting a material with intrinsic fluorescence lower than that of the other parts, in a total internal reflection microscope including a prism and the temperature controller. The present invention enables intrinsic fluorescence of the temperature controller to be suppressed, which allows highly sensitive fluorescence observation while controlling sample solution temperature with high precision. For instance, this in turn allows the throughput of single molecule DNA sequencing using a total internal reflection microscope to be improved.
摘要翻译：本发明的目的在于，在全内反射显微镜中，通过温度控制器控制样品溶液的温度并避免温度控制器的固有荧光，从而观察单分子荧光。本发明涉及在温度控制器的入射光和反射光通过的区域，以及采用具有比其他部分的荧光低的材料采取具有低于其它部分的固有荧光的材料的区域，在包含棱镜的全内反射显微镜中，温度控制器。本发明能够抑制温度控制器的固有荧光，从而高精度地控制样品溶液温度，从而实现高灵敏度的荧光观察。例如，这又允许改进使用全内反射显微镜的单分子DNA测序的生产量。

5. 发明授权

US08294122B2 Method for fluorescence analysis and fluorescence analyzer 有权
标题翻译：荧光分析方法和荧光分析仪
公开(公告)号：US08294122B2
公开(公告)日：2012-10-23
申请号：US12400321
申请日：2009-03-09
申请人： Takayuki Obara , Satoshi Takahashi , Akira Maekawa , Takuya Matsui , Nobutaka Kumazaki
发明人： Takayuki Obara , Satoshi Takahashi , Akira Maekawa , Takuya Matsui , Nobutaka Kumazaki
IPC分类号： G01N21/64
CPC分类号： G01N21/648 , G01N21/15 , G01N21/274 , G01N21/6452
摘要： The present invention aims at reducing background noise derived from a substance that is present in the vicinity of a target substance such as a DNA and protein and attached to the surface of a substrate without an effect on a fluorescent dye labeling the target substance. The substrate that has a probe and is capable of interacting with the target substance is irradiated with noise removing light such that an evanescent field is generated on the surface of the substrate. A target substance and a foreign particle, which are non-specifically stuck to the surface of the substrate, are decomposed by the evanescent field generated by the irradiation with the noise removing light. The evanescent field present near the surface of the substrate has almost no effect on the probe. It is possible to reduce the background noise derived from the substance that is present in the vicinity of the target substance and attached to the surface of the substrate and suppress effects on the probe and the target substance interacting with the probe.
摘要翻译：本发明旨在减少由存在于目标物质（例如DNA和蛋白质）附近的物质衍生的背景噪声，并附着于基材表面，而不影响标记目标物质的荧光染料。具有探针并且能够与目标物质相互作用的基板被消除噪声的光照射，使得在基板的表面上产生ev逝场。非特异性地粘附在基板表面上的目标物质和外来粒子被消除噪声的光照射产生的ev逝场分解。在衬底表面附近存在的消逝场对探针几乎没有影响。可以减少由存在于目标物质附近并附着于基板表面的物质导致的背景噪声，并抑制与探针相互作用的探针和目标物质的影响。

6. 发明申请

US20090242804A1 METHOD FOR FLUORESCENCE ANALYSIS AND FLUORESCENCE ANALYZER 有权
标题翻译：荧光分析和荧光分析仪的方法
公开(公告)号：US20090242804A1
公开(公告)日：2009-10-01
申请号：US12400321
申请日：2009-03-09
申请人： Takayuki OBARA , Satoshi Takahashi , Akira Maekawa , Takuya Matsui , Nobutaka Kumazaki
发明人： Takayuki OBARA , Satoshi Takahashi , Akira Maekawa , Takuya Matsui , Nobutaka Kumazaki
IPC分类号： G01N21/64
CPC分类号： G01N21/648 , G01N21/15 , G01N21/274 , G01N21/6452
摘要： The present invention aims at reducing background noise derived from a substance that is present in the vicinity of a target substance such as a DNA and protein and attached to the surface of a substrate without an effect on a fluorescent dye labeling the target substance. The substrate that has a probe and is capable of interacting with the target substance is irradiated with noise removing light such that an evanescent field is generated on the surface of the substrate. A target substance and a foreign particle, which are non-specifically stuck to the surface of the substrate, are decomposed by the evanescent field generated by the irradiation with the noise removing light. The evanescent field present near the surface of the substrate has almost no effect on the probe. It is possible to reduce the background noise derived from the substance that is present in the vicinity of the target substance and attached to the surface of the substrate and suppress effects on the probe and the target substance interacting with the probe.
摘要翻译：本发明旨在减少由存在于目标物质（例如DNA和蛋白质）附近的物质衍生的背景噪声，并附着于基材表面，而不影响标记目标物质的荧光染料。具有探针并且能够与目标物质相互作用的基板被消除噪声的光照射，使得在基板的表面上产生ev逝场。非特异性地粘附在基板表面上的目标物质和外来粒子被消除噪声的光照射产生的ev逝场分解。在衬底表面附近存在的消逝场对探针几乎没有影响。可以减少由存在于目标物质附近并附着于基板表面的物质导致的背景噪声，并抑制与探针相互作用的探针和目标物质的影响。

7. 发明授权

US09207235B2 Nucleic acid analyzer, reaction device for nucleic acid analysis and substrate of reaction device for nucleic acid analysis 有权
标题翻译：核酸分析仪，核酸分析反应装置和核酸分析反应装置底物
公开(公告)号：US09207235B2
公开(公告)日：2015-12-08
申请号：US13575564
申请日：2010-12-01
申请人： Yoshiaki Sugimura , Masatoshi Narahara , Kazumichi Imai , Toshiro Saito , Ryoji Inaba , Takuya Matsui
发明人： Yoshiaki Sugimura , Masatoshi Narahara , Kazumichi Imai , Toshiro Saito , Ryoji Inaba , Takuya Matsui
IPC分类号： B01L3/00 , G01N33/543 , G01N21/64 , G01N33/53 , G01N33/58 , G01N35/00
CPC分类号： G01N33/54313 , B01L3/502715 , B01L2200/0668 , B01L2300/0609 , B01L2300/0877 , B01L2300/0883 , G01N21/6452 , G01N21/648 , G01N33/5308 , G01N33/54373 , G01N33/582 , G01N35/00 , G01N2035/00564
摘要： Provided is a reaction device for nucleic acid analysis wherein microparticles, which carry a nucleic acid to be detected having been immobilized thereon, are aligned in a lattice form on a substrate according to the pixel size of a two-dimensional sensor. By this reaction device for nucleic acid analysis which is provided with a channel-forming reaction chamber on the substrate (101), the nucleic acid having been immobilized on the microparticles (103) on the substrate (101) is detected. The microparticles (103), which carry the nucleic acid to be detected having been immobilized thereon, are arranged by microstructures (102) aligned on the substrate (101).
摘要翻译：提供了用于核酸分析的反应装置，其中携带已被固定在其上的被检测核酸的微粒根据二维传感器的像素尺寸以格子形式排列在基板上。通过在基板（101）上设置有通道形成反应室的核酸分析用反应装置，检测固定在基板（101）上的微粒（103）上的核酸。携带已被固定在其上的被检测核酸的微粒（103）通过在基板（101）上排列的微结构（102）布置。

8. 发明授权

US07513214B2 Plasma processing method and apparatus 有权
标题翻译：等离子体处理方法和装置
公开(公告)号：US07513214B2
公开(公告)日：2009-04-07
申请号：US10920180
申请日：2004-08-18
申请人： Tomohiro Okumura , Hideo Haraguchi , Takuya Matsui , Izuru Matsuda , Akio Mitsuhashi
发明人： Tomohiro Okumura , Hideo Haraguchi , Takuya Matsui , Izuru Matsuda , Akio Mitsuhashi
IPC分类号： C23C16/00 , H05H1/46
CPC分类号： H01J37/32623 , C23C16/505
摘要： The interior of a vacuum chamber is maintained at a specified pressure by introducing a specified gas into the vacuum chamber having a plasma trap provided therein. Simultaneously, therewith, evacuation of the chamber is performed by a pump as an evacuating device, and a high-frequency power of 100 MHz is supplied to a counter electrode by counter-electrode use high-frequency power supply. Thus, uniform plasma is generated within the vacuum chamber, where plasma processing such as etching, deposition, and surface reforming can be carried out uniformly with a substrate placed on a substrate electrode.
摘要翻译：通过将特定的气体引入到其中设置有等离子体阱的真空室中，将真空室的内部保持在特定的压力。同时，通过作为排气装置的泵进行室的抽真空，并且通过对电极使用的高频电源将100MHz的高频功率提供给对电极。因此，在真空室内产生均匀的等离子体，其中等离子体处理如蚀刻，沉积和表面重整可以与放置在基板电极上的基板均匀地进行。

9. 发明授权

US08906671B2 Chilled reagent container and nucleic acid analyzer 有权
标题翻译：冷冻试剂容器和核酸分析仪
公开(公告)号：US08906671B2
公开(公告)日：2014-12-09
申请号：US13532308
申请日：2012-06-25
申请人： Motohiro Yamazaki , Ryoji Inaba , Shuhei Yamamoto , Takuya Matsui , Kohshi Maeda , Yuichiro Ota , Hiroyuki Higashino
发明人： Motohiro Yamazaki , Ryoji Inaba , Shuhei Yamamoto , Takuya Matsui , Kohshi Maeda , Yuichiro Ota , Hiroyuki Higashino
IPC分类号： C12M1/00 , C12M3/00 , C12M1/34 , G01N21/64 , G01N21/66 , B01L3/00
CPC分类号： B01L3/527 , B01L3/523 , B01L2200/0689 , B01L2200/141 , B01L2300/045 , B01L2300/1894
摘要： A chilled reagent container comprises a reagent vessel containing part for containing therein a plurality of reagent vessels, a container lid including a container lid hole through which the reagent vessels contained by the reagent vessel containing part are accessible, and a cooling block for cooling the reagent vessels contained by the reagent vessel containing part, wherein the container lid slides to be changeable between an opened situation wherein the reagent is accessible from an outside and a closed situation wherein the reagent is prevented from being accessed from the outside, wherein the chilled reagent container further comprises a reagent container packing including another hole through which the reagent vessels are accessible and arranged between the container lid and the reagent vessel containing part to be pressed against the container lid.
摘要翻译：冷冻试剂容器包括含有多个试剂容器的试剂容器的容纳部，容器盖，其具有可容纳由试剂容器容纳部容纳的试剂容器的容器盖孔，以及用于冷却试剂的冷却块所述试剂容器容纳部所容纳的容器，其中所述容器盖滑动，其中所述试剂可从外部进入的打开状态和所述试剂被阻止从外部进入的封闭状态之间变化，其中所述冷冻试剂容器还包括试剂容器填料，其包括另一孔，试剂容器可通过所述孔容纳在容器盖和待压靠在容器盖上的试剂容器容纳部分之间。

10. 发明申请

US20120329143A1 Chilled Reagent Container and Nucleic Acid Analyzer 有权
标题翻译：冷冻试剂容器和核酸分析仪
公开(公告)号：US20120329143A1
公开(公告)日：2012-12-27
申请号：US13532308
申请日：2012-06-25
申请人： Motohiro Yamazaki , Ryoji Inaba , Shuhei Yamamoto , Takuya Matsui , Kohshi Maeda , Yuichiro Ota , Hiroyuki Higashino
发明人： Motohiro Yamazaki , Ryoji Inaba , Shuhei Yamamoto , Takuya Matsui , Kohshi Maeda , Yuichiro Ota , Hiroyuki Higashino
IPC分类号： C12M1/34 , G01N21/64 , B01L3/00
CPC分类号： B01L3/527 , B01L3/523 , B01L2200/0689 , B01L2200/141 , B01L2300/045 , B01L2300/1894
摘要： A chilled reagent container comprises a reagent vessel containing part for containing therein a plurality of reagent vessels, a container lid including a container lid hole through which the reagent vessels contained by the reagent vessel containing part are accessible, anda cooling block for cooling the reagent vessels contained by the reagent vessel containing part, wherein the container lid slides to be changeable between an opened situation wherein the reagent is accessible from an outside and a closed situation wherein the reagent is prevented from being accessed from the outside, wherein the chilled reagent container further comprises a reagent container packing including another hole through which the reagent vessels are accessible and arranged between the container lid and the reagent vessel containing part to be pressed against the container lid.
摘要翻译：冷冻试剂容器包括含有多个试剂容器的试剂容器的容纳部，容器盖，其具有可容纳由试剂容器容纳部容纳的试剂容器的容器盖孔，以及用于冷却试剂的冷却块所述试剂容器容纳部所容纳的容器，其中所述容器盖滑动，其中所述试剂可从外部进入的打开状态和所述试剂被阻止从外部进入的封闭状态之间变化，其中所述冷冻试剂容器还包括试剂容器填料，其包括另一孔，试剂容器可通过所述孔容纳在容器盖和待压靠在容器盖上的试剂容器容纳部分之间。

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