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    • 2. 发明申请
    • PROTEIN-REFOLDING MATERIAL
    • 蛋白质重建材料
    • US20090005543A1
    • 2009-01-01
    • US12204496
    • 2008-09-04
    • Fujio MIZUKAMIYoshimichi KiyozumiTakuji IkedaAkiko KawaiTakako NagaseKengo SakaguchiHiroyuki Chiku
    • Fujio MIZUKAMIYoshimichi KiyozumiTakuji IkedaAkiko KawaiTakako NagaseKengo SakaguchiHiroyuki Chiku
    • C07K1/08C01B39/00B01J29/06
    • C07K1/1133C07K1/1136C07K14/245
    • The present invention provides a method for refolding protein produced, for example, by Escherichia coli, that is inactive due to an as yet unformed higher order structure, or protein deactivated due to a change in conformation for some reason. The invention comprises a method, refolding kit, refolding agent, and molding that activate a native function or activity inherent to a protein through treatment with zeolite beta of protein produced, for example, by Escherichia coli, that is inactive due to an as yet unformed higher order structure, or protein deactivated due to a change in conformation for some reason. The invention also comprises a method for producing an active protein that utilizes the same. As compared with conventional methods, the present invention can provide a novel method for activating protein function that is highly versatile and generalizable, that employs a simple and easy protocol, and that is inexpensive and enables repeated use of the function activator.
    • 本发明提供了一种重新折叠由例如大肠杆菌产生的蛋白质的方法,其由于尚未形成的高级结构而不活跃,或由于某种原因由于构象变化而失活的蛋白质。 本发明包括一种方法,重折叠试剂盒,重折叠剂和模制品,其通过用例如由大肠杆菌产生的蛋白质的β沸石处理而激活蛋白质固有的天然功能或活性,其由于尚未形成 由于某些原因由于构象变化而导致高阶结构或蛋白质失活。 本发明还包括一种制备利用其的活性蛋白质的方法。 与常规方法相比,本发明可以提供一种新颖的激活蛋白质功能的方法,该蛋白质功能具有高度通用性和可广泛性,采用简单易用的方案,价格便宜,能重复使用功能活化剂。
    • 3. 发明申请
    • Protein-refolding material
    • 蛋白质重折叠材料
    • US20060194279A1
    • 2006-08-31
    • US10563870
    • 2004-07-07
    • Fujio MizukamiYoshimichi KiyozumiTakuji IkedaAkiko KawaiTakako NagaseKengo SakaguchiHiroyuki Chiku
    • Fujio MizukamiYoshimichi KiyozumiTakuji IkedaAkiko KawaiTakako NagaseKengo SakaguchiHiroyuki Chiku
    • C12P21/06C07K14/47C07K14/195C12N9/00
    • C07K1/1133C07K1/1136C07K14/245
    • The present invention provides a method for refolding protein produced, for example, by Escherichia coli, that is inactive due to an as yet unformed higher order structure, or protein deactivated due to a change in conformation for some reason. The invention comprises a method, refolding kit, refolding agent, and molding that activate a native function or activity inherent to a protein through treatment with zeolite beta of protein produced, for example, by Escherichia coli, that is inactive due to an as yet unformed higher order structure, or protein deactivated due to a change in conformation for some reason. The invention also comprises a method for producing an active protein that utilizes the same. As compared with conventional methods, the present invention can provide a novel method for activating protein function that is highly versatile and generalizable, that employs a simple and easy protocol, and that is inexpensive and enables repeated use of the function activator.
    • 本发明提供了一种重新折叠由例如大肠杆菌产生的蛋白质的方法,其由于尚未形成的高级结构而不活跃,或由于某种原因由于构象变化而失活的蛋白质。 本发明包括一种方法,重折叠试剂盒,重折叠剂和模制品,其通过用例如由大肠杆菌产生的蛋白质的β沸石处理而激活蛋白质固有的天然功能或活性,其由于尚未形成 由于某些原因由于构象变化而导致高阶结构或蛋白质失活。 本发明还包括一种制备利用其的活性蛋白质的方法。 与常规方法相比,本发明可以提供一种新颖的激活蛋白质功能的方法,该蛋白质功能具有高度通用性和可广泛性,采用简单易用的方案,价格便宜,能重复使用功能活化剂。
    • 4. 发明授权
    • Method of manufacturing crystalline microporous material
    • 制造结晶微孔材料的方法
    • US06022519A
    • 2000-02-08
    • US322687
    • 1999-05-28
    • Shinichi ShimizuFujio MizukamiYoshimichi Kiyozumi
    • Shinichi ShimizuFujio MizukamiYoshimichi Kiyozumi
    • B01J29/035B01J29/072B01J29/40B01J37/02C01B37/02C01B39/02C01B39/04C01B39/26C01B39/38C01B39/46C01B33/021C01B39/36C01B39/40
    • B01J29/40B01J29/035B01J29/072B01J37/0236C01B37/02C01B39/02C01B39/04C01B39/265C01B39/38C01B39/46B01J2229/26B01J2229/36B01J2229/42Y10S423/22Y10S423/25Y10S423/29
    • A method of manufacturing crystalline microporous material suitable for manufacturing a variety of crystalline microporous material under mild reaction conditions and under safe working environmental conditions by using simple installation. The crystalline microporous materials obtained by the present invention may be used as adsorbent agent, catalyst or separating material. According to the characterizing features of the manufacturing method of the present invention, there are provided a method of using cation compound or amine as crystallizing modifier and using, in combination, raw material including canemite or silicon dioxide and a further method using raw material including silicon dioxide and aluminum salt. The method includes a step of mixing these components, a solid-liquid separating step for separating produced fine particles, and a crystallizing step for crystallizing the separated solid component. The invention achieves improvement in safety, economy and so on, in comparison with the coventionally practiced hydrothermal synthesis method which requires severe reaction conditions of high-temperature, high-pressure and strong alkaline. Further, the method allows freedom in adjustment of the pore diameter and allows a higher proportion of porous structure to be maintained in a product when the material is formed into a molded product.
    • 一种制造结晶微孔材料的方法,适用于在温和的反应条件下,在安全的工作环境条件下,通过简单的安装制造各种结晶微孔材料。 通过本发明获得的结晶微孔材料可以用作吸附剂,催化剂或分离材料。 根据本发明的制造方法的特征,提供了使用阳离子化合物或胺作为结晶改性剂并且组合使用包括二硅酸钙的原料的方法,以及使用包括硅的原料的另一种方法 二氧化物和铝盐。 该方法包括混合这些组分的步骤,用于分离生成的细颗粒的固液分离步骤和用于使分离的固体组分结晶的结晶步骤。 与需要高温,高压,强碱性苛刻反应条件的常规水热合成方法相比,本发明实现了安全性,经济性等方面的改进。 此外,该方法允许调节孔径的自由度,并且当材料形成成型产品时允许在产品中保持较高比例的多孔结构。
    • 5. 发明授权
    • Method of manufacturing crystalline microporous materials
    • 制造结晶微孔材料的方法
    • US06270743B1
    • 2001-08-07
    • US09469438
    • 1999-12-21
    • Shinichi ShimizuFujio MizukamiYoshimichi Kiyozumi
    • Shinichi ShimizuFujio MizukamiYoshimichi Kiyozumi
    • C01B3902
    • C01B39/02B01J29/035B01J29/072B01J29/40B01J37/0236B01J2229/26B01J2229/36B01J2229/42C01B37/02C01B39/04C01B39/265C01B39/38C01B39/46Y10S423/22Y10S423/25Y10S423/29
    • A method of manufacturing crystalline microporous material suitable for manufacturing a variety of crystalline microporous material under mild reaction conditions and under safe working environmental conditions by using simple installation. The crystalline microporous materials obtained by the present invention may be used as adsorbent agent, catalyst or separating material. According to the characterizing features of the manufacturing method of the present invention, there are provided a method of using cation compound or amine as crystallizing modifier and using, in combination, raw material including kanemite or silicon dioxide and a further method using raw material including silicon dioxide and aluminum salt. The method includes a step of mixing these components, a solid-liquid separating step for separating produced fine particles, and a crystallizing step for crystallizing the separated solid component. The invention achieves improvement in safety, economy and so on, in comparison with the coventionally practiced hydrothermal synthesis method which requires severe reaction conditions of high-temperature, high-pressure and strong alkaline. Further, the method allows freedom in adjustment of the pore diameter and allows a higher proportion of porous structure to be maintained in a product when the material is formed into a molded product.
    • 一种制造结晶微孔材料的方法,适用于在温和的反应条件下,在安全的工作环境条件下,通过简单的安装制造各种结晶微孔材料。 通过本发明获得的结晶微孔材料可以用作吸附剂,催化剂或分离材料。 根据本发明的制造方法的特征,提供了一种使用阳离子化合物或胺作为结晶改性剂的方法,并且组合使用包含卡米质或二氧化硅的原料,以及使用包含硅的原料的另一种方法 二氧化物和铝盐。 该方法包括混合这些组分的步骤,用于分离生成的细颗粒的固液分离步骤和用于使分离的固体组分结晶的结晶步骤。 与需要高温,高压,强碱性苛刻反应条件的常规水热合成方法相比,本发明实现了安全性,经济性等方面的改进。 此外,该方法允许调节孔径的自由度,并且当材料形成成型产品时允许在产品中保持较高比例的多孔结构。