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    • 1. 发明申请
    • Method of amplifying nucleic acid and apparatus therefor
    • 扩增核酸的方法及其设备
    • US20060147921A1
    • 2006-07-06
    • US10524263
    • 2002-11-13
    • Takayuki KandaSinichi FukuzonoSatoshi Takahashi
    • Takayuki KandaSinichi FukuzonoSatoshi Takahashi
    • C12Q1/68C12P19/34C07H21/04
    • C12Q1/6851B01L7/52C12Q1/6844C12Q2527/137
    • In nucleic acid amplification, the amount of a labeled or modified oligomer used is reduced to lower cost, and detection is enabled within the measurement range of a detection unit without requiring operations such as dilution. A nucleic acid measurement method of the invention comprises the steps of extracting a target nucleic acid, mixing an oligomer labeled with a luminescent substance or a modification group with another oligomer that has the same base sequence with that of the labeled oligomer and that is not labeled with a luminescent substance or a modification group, amplifying the target nucleic acid using the mixture of the labeled oligomer and the unlabeled oligomer, adding a luminescent substance to an oligomer labeled with a modification group and its amplification product, and measuring the amplified target nucleic acid using a photodetector.
    • 在核酸扩增中,使用的标记或修饰的低聚物的量降低到较低成本,并且在检测单元的测量范围内能够进行检测,而不需要诸如稀释的操作。 本发明的核酸测定方法包括以下步骤:提取靶核酸,将用发光物质或修饰基团标记的寡聚物与标记的寡聚体具有相同碱基序列的另一低聚物混合并且未标记 使用发光物质或修饰基团,使用标记的寡聚体和未标记的寡聚体的混合物来扩增靶核酸,向发现的修饰基团标记的寡聚物及其扩增产物中加入发光物质,并测定扩增的靶核酸 使用光电检测器。