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1. 发明申请

US20070298415A1 Method of Amplifying Nucleic Acid 审中-公开
标题翻译：核酸扩增方法
公开(公告)号：US20070298415A1
公开(公告)日：2007-12-27
申请号：US10582345
申请日：2004-12-06
申请人： Takashi Uemori , Osamu Takeda , Junko Yamamoto , Hiroyuki Mukai , Kiyozo Asada , Ikunoshin Kato
发明人： Takashi Uemori , Osamu Takeda , Junko Yamamoto , Hiroyuki Mukai , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C12P19/34 , C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2531/119 , C12Q2525/185 , C12Q2537/143 , C12Q2525/161
摘要： In the case where a template nucleic acid has sequences substantially the same as each other, a primer is designed in a region between these sequences so as to give a product in a ladder structure in which a target region is polymerized in a single sequence via the same sequences in the ICAN reaction. By using a chimeric oligonucleotide primer and a ladder-forming oligonucleotide primer containing a specific base sequence, an amplification product having a ladder structure can be positively formed and thus the sensitivity, amplification efficiency and reaction speed in the ICAN reaction can be elevated.
摘要翻译：在模板核酸具有彼此基本相同的序列的情况下，在这些序列之间的区域中设计引物，以便产生梯形结构中的产物，其中靶区域通过经由在ICAN反应中相同的序列。通过使用嵌合寡核苷酸引物和含有特定碱基序列的梯形寡核苷酸引物，可以积极地形成具有梯形结构的扩增产物，从而可以提高ICAN反应中的灵敏度，扩增效率和反应速度。

2. 发明授权

US06951722B2 Method for amplifying nucleic acid sequence 失效
公开(公告)号：US06951722B2
公开(公告)日：2005-10-04
申请号：US09935338
申请日：2001-08-23
申请人： Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
发明人： Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C07H21/04 , C12N9/22 , C12P19/34 , C12Q1/68
CPC分类号： C12N9/1252 , C12P19/34 , C12Q1/6853 , C12Q2531/119 , C12Q2525/121 , C12Q2521/301
摘要： A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.

3. 发明申请

US20050239100A1 Method for amplifying nucleic acid sequence 审中-公开
标题翻译：扩增核酸序列的方法
公开(公告)号：US20050239100A1
公开(公告)日：2005-10-27
申请号：US10973919
申请日：2004-10-27
申请人： Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
发明人： Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C07H21/04 , C12N9/22 , C12P19/34 , C12Q1/68
CPC分类号： C12N9/1252 , C12P19/34 , C12Q1/6853 , C12Q2531/119 , C12Q2525/121 , C12Q2521/301
摘要： A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.
摘要翻译：一种用于扩增核酸序列的方法和方法，其特征在于在嵌合寡核苷酸引物存在下进行DNA合成反应; 提供大量DNA扩增片段的方法; 通过将上述方法与另一种核酸序列扩增方法结合来扩增核酸序列的有效方法; 用于检测用于检测或定量微生物如病毒，细菌，真菌或酵母的核酸序列的方法; 以及通过上述方法原位检测DNA扩增片段的方法。

4. 发明申请

US20050123950A1 Method for amplifying nucleic acid sequence 审中-公开
公开(公告)号：US20050123950A1
公开(公告)日：2005-06-09
申请号：US10929759
申请日：2004-08-31
申请人： Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
发明人： Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C07H21/04 , C12N9/22 , C12P19/34 , C12Q1/68
CPC分类号： C12N9/1252 , C12P19/34 , C12Q1/6853 , C12Q2531/119 , C12Q2525/121 , C12Q2521/301
摘要： A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.

5. 发明申请

US20050059000A1 Method of stabilizing reagent for amplifying or detecting nucleic acid and storage method 审中-公开
标题翻译：稳定核酸扩增或检测试剂的方法及储存方法
公开(公告)号：US20050059000A1
公开(公告)日：2005-03-17
申请号：US10478633
申请日：2002-06-12
申请人： Hiroaki Sagawa , Takashi Uemori , Hiroyuki Mukai , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Kiyozo Asada , Ikunoshin Kato
发明人： Hiroaki Sagawa , Takashi Uemori , Hiroyuki Mukai , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q2521/327 , C12Q2525/121 , C12Q2527/137
摘要： A method of stabilizing a reaction reagent for highly sensitively and specifically amplifying a target nucleic acid in a sample with the use of a chimeric oligonucleotide primer and a method of storing the same over a long time; and a method of highly sensitively detecting a pathogenic microorganism and a virus.
摘要翻译：使用嵌合寡核苷酸引物对样品中的靶核酸进行高灵敏度和特异性扩增的反应试剂的稳定化方法及其长时间存储的方法; 以及高敏感性检测病原微生物和病毒的方法。

6. 发明申请

US20130295576A1 METHOD FOR MODIFYING NUCLEIC ACIDS 有权
标题翻译：修饰核酸的方法
公开(公告)号：US20130295576A1
公开(公告)日：2013-11-07
申请号：US13980970
申请日：2012-01-20
申请人： Junko Yamamoto , Keiko Kubo , Takashi Uemori , Hiroyuki Mukai , Kiyozo Asada
发明人： Junko Yamamoto , Keiko Kubo , Takashi Uemori , Hiroyuki Mukai , Kiyozo Asada
IPC分类号： C12Q1/68
CPC分类号： C12N15/01 , C12Q1/6848 , C12Q1/686 , C12Q1/689 , C12Q2523/101 , C12Q2525/117
摘要： The present invention pertains to: a method for modifying a nucleic acid contained in a sample, said method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, said method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.
摘要翻译：本发明涉及：用于修饰样品中所含核酸的方法，所述方法包括在酸性多糖和/或核苷酸存在下使样品与核酸改性剂接触的步骤; 以及选择性地检测来自样品中含有的活细胞的核酸的方法，所述方法包括以下步骤：（a）根据包含在所述样品中的核酸的方法修饰样品中的核酸的步骤样品，其包括在酸性多糖和/或核苷酸的存在下使样品与核酸改性剂接触的步骤; 和（b）在步骤（a）之后从样品中选择性地检测未修饰的核酸的步骤。本发明还涉及用于这些方法的试剂盒和组合物。

7. 发明授权

US09458498B2 Method for modifying nucleic acids 有权
标题翻译：核酸修饰方法
公开(公告)号：US09458498B2
公开(公告)日：2016-10-04
申请号：US13980970
申请日：2012-01-20
申请人： Junko Yamamoto , Keiko Kubo , Takashi Uemori , Hiroyuki Mukai , Kiyozo Asada
发明人： Junko Yamamoto , Keiko Kubo , Takashi Uemori , Hiroyuki Mukai , Kiyozo Asada
IPC分类号： C12Q1/68 , C12N15/01
CPC分类号： C12N15/01 , C12Q1/6848 , C12Q1/686 , C12Q1/689 , C12Q2523/101 , C12Q2525/117
摘要： The present invention pertains to: a method for modifying a nucleic acid contained in a sample, the method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, the method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.
摘要翻译：本发明涉及：用于修饰样品中所含的核酸的方法，所述方法包括在酸性多糖和/或核苷酸的存在下使样品与核酸改性剂接触的步骤; 以及选择性检测来自样品中含有的活细胞的核酸的方法，所述方法包括以下步骤：（a）根据包含在所述样品中的核酸的方法修饰样品中的核酸的步骤样品，其包括在酸性多糖和/或核苷酸的存在下使样品与核酸改性剂接触的步骤; 和（b）在步骤（a）之后从样品中选择性地检测未修饰的核酸的步骤。本发明还涉及用于这些方法的试剂盒和组合物。

8. 发明申请

US20130122503A1 METHOD FOR PRODUCING RNA-CONTAINING PROBE FOR DETECTING A TARGET NUCLEOTIDE 有权
标题翻译：用于生产用于检测目标核酸的含RNA探针的方法
公开(公告)号：US20130122503A1
公开(公告)日：2013-05-16
申请号：US13812242
申请日：2011-07-28
申请人： Junko Yamamoto , Tomoo Inden , Toshiharu Ohba , Tooru Suzuki , Hiroyuki Mukai , Kiyozo Asada
发明人： Junko Yamamoto , Tomoo Inden , Toshiharu Ohba , Tooru Suzuki , Hiroyuki Mukai , Kiyozo Asada
IPC分类号： G06F19/12 , C12Q1/68
CPC分类号： G06F19/12 , C12Q1/6816 , C12Q1/6876 , C12Q2527/107 , G06F19/20
摘要： An object of the present invention is to provide a simple and useful method for producing an RNA-containing probe for detecting a target nucleotide, a simple and useful method, device, and system for processing nucleotide sequence information, and a simple and useful method for detecting a target nucleotide. The present invention provides a method for processing nucleotide sequence information, the method comprising the step of generating partial nucleotide sequences which has 7 to 14 nucleotides and a Tm value of 25 to 40° C. and in which a target nucleotide or a nucleotide adjacent to the target nucleotide is located at a position between 3 and 5 nucleotides from the 3′ or 5′ end. The method according to the present invention is useful for simply and efficiently producing an RNA-containing probe for detecting a target nucleic acid, without the basis of researchers' experiences or guess, and are extremely useful not only in the field of genetic engineering, but also in the field of medical research.
摘要翻译：本发明的目的在于提供一种用于检测靶核苷酸的含RNA探针的简单且有用的方法，用于处理核苷酸序列信息的简单有用的方法，装置和系统，以及简单有用的方法检测靶核苷酸。本发明提供了一种处理核苷酸序列信息的方法，该方法包括产生具有7-14个核苷酸并且Tm值为25至40℃的部分核苷酸序列的步骤，其中靶核苷酸或邻近靶核苷酸位于3'或5'端3〜5个核苷酸的位置。根据本发明的方法可用于简单且有效地制备用于检测靶核酸的含RNA探针，而无需基于研究人员的经验或猜测，并且不仅在遗传工程领域中非常有用，而且也在医学研究领域。

9. 发明授权

US09336349B2 Method for producing RNA-containing probe for detecting a target nucleotide 有权
标题翻译：用于产生用于检测靶核苷酸的含RNA探针的方法
公开(公告)号：US09336349B2
公开(公告)日：2016-05-10
申请号：US13812242
申请日：2011-07-28
申请人： Junko Yamamoto , Tomoo Inden , Toshiharu Ohba , Tooru Suzuki , Hiroyuki Mukai , Kiyozo Asada
发明人： Junko Yamamoto , Tomoo Inden , Toshiharu Ohba , Tooru Suzuki , Hiroyuki Mukai , Kiyozo Asada
IPC分类号： C12Q1/68 , G06F19/12 , G06F19/20
CPC分类号： G06F19/12 , C12Q1/6816 , C12Q1/6876 , C12Q2527/107 , G06F19/20
摘要： An object of the present invention is to provide a simple and useful method for producing an RNA-containing probe for detecting a target nucleotide, a simple and useful method, device, and system for processing nucleotide sequence information, and a simple and useful method for detecting a target nucleotide. The present invention provides a method for processing nucleotide sequence information, the method comprising the step of generating partial nucleotide sequences which has 7 to 14 nucleotides and a Tm value of 25 to 40° C. and in which a target nucleotide or a nucleotide adjacent to the target nucleotide is located at a position between 3 and 5 nucleotides from the 3′ or 5′ end. The method according to the present invention is useful for simply and efficiently producing an RNA-containing probe for detecting a target nucleic acid, without the basis of researchers' experiences or guess, and are extremely useful not only in the field of genetic engineering, but also in the field of medical research.
摘要翻译：本发明的目的在于提供一种用于检测靶核苷酸的含RNA探针的简单且有用的方法，用于处理核苷酸序列信息的简单有用的方法，装置和系统，以及简单有用的方法检测靶核苷酸。本发明提供了一种处理核苷酸序列信息的方法，该方法包括产生具有7-14个核苷酸并且Tm值为25至40℃的部分核苷酸序列的步骤，其中靶核苷酸或邻近靶核苷酸位于3'或5'端3〜5个核苷酸的位置。根据本发明的方法可用于简单且有效地制备用于检测靶核酸的含RNA探针，而无需基于研究人员的经验或猜测，并且不仅在遗传工程领域中非常有用，而且也在医学研究领域。

10. 发明申请

US20050153326A1 Method for synthesizing cDNA 审中-公开
标题翻译： cDNA合成方法
公开(公告)号：US20050153326A1
公开(公告)日：2005-07-14
申请号：US10995005
申请日：2004-11-23
申请人： Junko Yamamoto , Kazue Miyake , Hiroyuki Mukai , Fumitsugu Hino , Ikunoshin Kato
发明人： Junko Yamamoto , Kazue Miyake , Hiroyuki Mukai , Fumitsugu Hino , Ikunoshin Kato
IPC分类号： C12N15/10 , C12Q1/68 , C12N9/22 , C12P19/34
CPC分类号： C12Q1/6844 , C12N15/10 , C12Q1/686 , C12Q2521/319 , C12Q2521/107
摘要： A method for synthesizing cDNA characterized by performing a reverse transcription reaction in the presence of an enzyme having a reverse transcriptional activity and another enzyme different from the former one which as a 3′-5′ exonuclease activity.
摘要翻译：一种合成cDNA的方法，其特征在于在具有逆转录活性的酶的存在下进行逆转录反应，另一种不同于前者的3'-5'核酸外切酶活性的酶。

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